This is a Validated Antibody Database (VAD) review about mouse Bcar1, based on 17 published articles (read how Labome selects the articles), using Bcar1 antibody in all methods. It is aimed to help Labome visitors find the most suited Bcar1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Bcar1 synonym: AI385681; Cas; Crkas

Knockout validation
BD Biosciences
mouse monoclonal (21/p130[Cas)
  • western blot knockout validation; mouse; loading ...; fig 2a
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s3b
  • immunocytochemistry; mouse; loading ...; fig 1d
  • immunocytochemistry; human; loading ...; fig s5a
BD Biosciences Bcar1 antibody (BD, 610271) was used in western blot knockout validation on mouse samples (fig 2a), in immunohistochemistry - paraffin section on mouse samples (fig s3b), in immunocytochemistry on mouse samples (fig 1d) and in immunocytochemistry on human samples (fig s5a). Sci Adv (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (35B.1A4)
  • immunoprecipitation; human; 1:500; loading ...; fig 4b
Santa Cruz Biotechnology Bcar1 antibody (Santa cruz, sc-20029) was used in immunoprecipitation on human samples at 1:500 (fig 4b). Int J Biol Sci (2021) ncbi
mouse monoclonal (35B.1A4)
  • immunoprecipitation; human; fig 2s2
In order to analyze Src homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases by time-resolved multimodal analysis, Santa Cruz Biotechnology Bcar1 antibody (Santa Cruz Biotechnology, sc-20029) was used in immunoprecipitation on human samples (fig 2s2). elife (2016) ncbi
mouse monoclonal (35B.1A4)
  • immunocytochemistry; human; fig 6
  • western blot; human; fig 3
Santa Cruz Biotechnology Bcar1 antibody (Santa Cruz, sc-20029) was used in immunocytochemistry on human samples (fig 6) and in western blot on human samples (fig 3). Cancer Lett (2015) ncbi
Abcam
mouse monoclonal (M144)
  • immunohistochemistry - paraffin section; human; 1:150; fig 2
Abcam Bcar1 antibody (Abcam, ab31831) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 2). Mol Med Rep (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6b, 6c
Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4011) was used in western blot on mouse samples at 1:1000 (fig 6b, 6c). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s9b
In order to examine whether p140Cap protein levels in ERBB2-amplified breast cancer patients correlate with survival, Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4011) was used in western blot on human samples at 1:1000 (fig s9b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s1
In order to elucidate initiation of directional invasion via the action of Rap1 GTPase as a tension sensor by applied stretch, Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4015) was used in western blot on human samples at 1:1000 (fig s1). J Cell Sci (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig 8
In order to elucidate how spindle orientation occurs through a ligand-independent intgrin beta 1 mechanosensory complex, Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4015) was used in immunocytochemistry on human samples at 1:50 (fig 8). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4015) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, Cell Signaling Technology Bcar1 antibody (Cell Signaling, 4011S) was used in western blot on mouse samples at 1:1000 (fig 5). elife (2016) ncbi
BD Biosciences
mouse monoclonal (21/p130[Cas)
  • western blot; human; loading ...; fig 2b
BD Biosciences Bcar1 antibody (BD Pharmigen, 610271) was used in western blot on human samples (fig 2b). elife (2020) ncbi
mouse monoclonal (21/p130[Cas)
  • western blot knockout validation; mouse; loading ...; fig 2a
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s3b
  • immunocytochemistry; mouse; loading ...; fig 1d
  • immunocytochemistry; human; loading ...; fig s5a
BD Biosciences Bcar1 antibody (BD, 610271) was used in western blot knockout validation on mouse samples (fig 2a), in immunohistochemistry - paraffin section on mouse samples (fig s3b), in immunocytochemistry on mouse samples (fig 1d) and in immunocytochemistry on human samples (fig s5a). Sci Adv (2019) ncbi
mouse monoclonal (21/p130[Cas)
  • immunocytochemistry; human; loading ...; fig 5f
  • western blot; human; loading ...; fig 5a
BD Biosciences Bcar1 antibody (BD Bioscience, 610271) was used in immunocytochemistry on human samples (fig 5f) and in western blot on human samples (fig 5a). PLoS Pathog (2016) ncbi
mouse monoclonal (21/p130[Cas)
  • western blot; human; loading ...; fig 2a
BD Biosciences Bcar1 antibody (BD Biosciences, 21) was used in western blot on human samples (fig 2a). Oncogene (2017) ncbi
mouse monoclonal (21/p130[Cas)
  • western blot; human; 1:1000; fig 5
In order to determine how modulation of FAK and Src adhesion signalling occurs without the need for adhesion complex composition, BD Biosciences Bcar1 antibody (BD Biosciences, 610272) was used in western blot on human samples at 1:1000 (fig 5). J Cell Biol (2016) ncbi
monoclonal
  • western blot; human; fig 4
In order to characterize an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications defined by PLEKHA7, BD Biosciences Bcar1 antibody (BD Transduction Laboratories, p27820) was used in western blot on human samples (fig 4). Cell Cycle (2016) ncbi
monoclonal
  • western blot; mouse; 1:1000; fig 5
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, BD Biosciences Bcar1 antibody (Transduction Labs, P27820) was used in western blot on mouse samples at 1:1000 (fig 5). elife (2016) ncbi
mouse monoclonal (21/p130[Cas)
  • western blot; human
BD Biosciences Bcar1 antibody (BD Signal Transduction Laboratories, 610271) was used in western blot on human samples . Cell Microbiol (2014) ncbi
Articles Reviewed
  1. Mao C, Jiang S, Wang X, Tao S, Jiang B, Mao C, et al. BCAR1 plays critical roles in the formation and immunoevasion of invasive circulating tumor cells in lung adenocarcinoma. Int J Biol Sci. 2021;17:2461-2475 pubmed publisher
  2. Shaaya M, Fauser J, Zhurikhina A, Conage Pough J, Huyot V, Brennan M, et al. Light-regulated allosteric switch enables temporal and subcellular control of enzyme activity. elife. 2020;9: pubmed publisher
  3. Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
  4. Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, et al. Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity. Sci Adv. 2019;5:eaau7802 pubmed publisher
  5. Grasso S, Chapelle J, Salemme V, Aramu S, Russo I, Vitale N, et al. The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nat Commun. 2017;8:14797 pubmed publisher
  6. Kumar B, Dutta D, Iqbal J, Ansari M, Roy A, Chikoti L, et al. ESCRT-I Protein Tsg101 Plays a Role in the Post-macropinocytic Trafficking and Infection of Endothelial Cells by Kaposi's Sarcoma-Associated Herpesvirus. PLoS Pathog. 2016;12:e1005960 pubmed publisher
  7. Frank S, Köllmann C, van Lidth de Jeude J, Thiagarajah J, Engelholm L, Frödin M, et al. The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion. Oncogene. 2017;36:1816-1828 pubmed publisher
  8. Freeman S, Christian S, Austin P, Iu I, Graves M, Huang L, et al. Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor. J Cell Sci. 2017;130:152-163 pubmed publisher
  9. Jadwin J, Oh D, Curran T, Ogiue Ikeda M, Jia L, White F, et al. Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinases. elife. 2016;5:e11835 pubmed publisher
  10. Petridou N, Skourides P. A ligand-independent integrin β1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
  11. West J, Carrier E, Bloodworth N, Schroer A, Chen P, Ryzhova L, et al. Serotonin 2B Receptor Antagonism Prevents Heritable Pulmonary Arterial Hypertension. PLoS ONE. 2016;11:e0148657 pubmed publisher
  12. Horton E, Humphries J, Stutchbury B, Jacquemet G, Ballestrem C, Barry S, et al. Modulation of FAK and Src adhesion signaling occurs independently of adhesion complex composition. J Cell Biol. 2016;212:349-64 pubmed publisher
  13. Kourtidis A, Anastasiadis P. PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications. Cell Cycle. 2016;15:498-505 pubmed publisher
  14. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  15. Yuan L, Liu X. Platelets are associated with xenograft tumor growth and the clinical malignancy of ovarian cancer through an angiogenesis-dependent mechanism. Mol Med Rep. 2015;11:2449-58 pubmed publisher
  16. Ward J, Ha J, Jayaraman M, Dhanasekaran D. LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix. Cancer Lett. 2015;356:382-91 pubmed publisher
  17. Eucker T, Samuelson D, Hunzicker Dunn M, Konkel M. The focal complex of epithelial cells provides a signalling platform for interleukin-8 induction in response to bacterial pathogens. Cell Microbiol. 2014;16:1441-55 pubmed publisher