This is a Validated Antibody Database (VAD) review about mouse Atxn3, based on 18 published articles (read how Labome selects the articles), using Atxn3 antibody in all methods. It is aimed to help Labome visitors find the most suited Atxn3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atxn3 synonym: 2210008M02Rik; AI463012; AI647473; ATX3; MJD1; Mjd; Sca3; ataxin-3; ataxin-3; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; machado-Joseph disease protein 1 homolog

Knockout validation
EMD Millipore
mouse monoclonal (1H9)
  • western blot knockout validation; mouse; loading ...; fig 2A
  • western blot; human; loading ...; fig S2A
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot knockout validation on mouse samples (fig 2A) and in western blot on human samples (fig S2A). Nucleic Acids Res (2017) ncbi
EMD Millipore
mouse monoclonal (1H9)
  • western blot knockout validation; mouse; 1:20,000; fig 2
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot knockout validation on mouse samples at 1:20,000 (fig 2). Cell Stress Chaperones (2012) ncbi
Proteintech Group
rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig 1d
Proteintech Group Atxn3 antibody (Proteintech, 13505?C1-AP) was used in proximity ligation assay on human samples (fig 1d). PLoS Genet (2015) ncbi
EMD Millipore
mouse monoclonal (1H9)
  • western blot; mouse; 1:1000; loading ...; fig 4b
EMD Millipore Atxn3 antibody (Merck Millipore, 1H9) was used in western blot on mouse samples at 1:1000 (fig 4b). Proc Natl Acad Sci U S A (2018) ncbi
mouse monoclonal (1H9)
  • western blot; mouse; loading ...; fig 1a
  • immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 4b
In order to research the effect of polyglutamine tracts on the autophagy process, EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on mouse samples (fig 1a), in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 4b). Nature (2017) ncbi
mouse monoclonal (1H9)
  • western blot knockout validation; mouse; loading ...; fig 2A
  • western blot; human; loading ...; fig S2A
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot knockout validation on mouse samples (fig 2A) and in western blot on human samples (fig S2A). Nucleic Acids Res (2017) ncbi
mouse monoclonal (1H9)
  • immunocytochemistry; human; 1:1000; loading ...; tbl s4
  • western blot; human; 1:1000; loading ...; tbl s4
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in immunocytochemistry on human samples at 1:1000 (tbl s4) and in western blot on human samples at 1:1000 (tbl s4). Stem Cell Res (2016) ncbi
mouse monoclonal (1H9)
  • western blot; human; 1:1000; loading ...; tbl 2
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on human samples at 1:1000 (tbl 2). Stem Cell Res (2016) ncbi
mouse monoclonal (1H9)
  • western blot; human; 1:1000; loading ...; fig 1f
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on human samples at 1:1000 (fig 1f). Stem Cell Res (2016) ncbi
mouse monoclonal (1H9)
  • western blot; human; 1:1000; loading ...; fig 2d
EMD Millipore Atxn3 antibody (Millipore, 1H9) was used in western blot on human samples at 1:1000 (fig 2d). J Cell Biol (2016) ncbi
mouse monoclonal (1H9)
  • immunohistochemistry - paraffin section; human; 1:200
  • immunoprecipitation; human
  • western blot; human; 1:1000
  • immunohistochemistry - free floating section; mouse; 1:1000
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in immunohistochemistry - paraffin section on human samples at 1:200, in immunoprecipitation on human samples , in western blot on human samples at 1:1000 and in immunohistochemistry - free floating section on mouse samples at 1:1000. Neurobiol Dis (2015) ncbi
mouse monoclonal (1H9)
  • western blot; African green monkey; 1:10,000; loading ...; fig 1
EMD Millipore Atxn3 antibody (Merck Millipore, MAB5360) was used in western blot on African green monkey samples at 1:10,000 (fig 1). Biochim Biophys Acta (2015) ncbi
mouse monoclonal (1H9)
  • western blot; mouse; 1:500; fig 2
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on mouse samples at 1:500 (fig 2). elife (2015) ncbi
mouse monoclonal (1H9)
  • western blot; human; loading ...; fig 3a
EMD Millipore Atxn3 antibody (Millipore, Mab5360) was used in western blot on human samples (fig 3a). PLoS Genet (2015) ncbi
mouse monoclonal (1H9)
  • immunocytochemistry; rat; 1:300
  • western blot; human; 1:1000
EMD Millipore Atxn3 antibody (Millipore Corporation, MAB5360) was used in immunocytochemistry on rat samples at 1:300 and in western blot on human samples at 1:1000. J Neurochem (2014) ncbi
mouse monoclonal (1H9)
  • western blot; human
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on human samples . Biochemistry (2013) ncbi
mouse monoclonal (1H9)
  • western blot; human
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
mouse monoclonal (1H9)
  • western blot; human
EMD Millipore Atxn3 antibody (Chemicon Millipore, MAB5360) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
mouse monoclonal (1H9)
  • immunohistochemistry; mouse; 1:4000
  • western blot; mouse; 1:2500
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in immunohistochemistry on mouse samples at 1:4000 and in western blot on mouse samples at 1:2500. PLoS ONE (2013) ncbi
mouse monoclonal (1H9)
  • immunohistochemistry; mouse; 1:1000
  • western blot; human; 1:4000
EMD Millipore Atxn3 antibody (Millipore, MAB 5360) was used in immunohistochemistry on mouse samples at 1:1000 and in western blot on human samples at 1:4000. Hum Mol Genet (2013) ncbi
mouse monoclonal (1H9)
  • western blot knockout validation; mouse; 1:20,000; fig 2
EMD Millipore Atxn3 antibody (Millipore, MAB5360) was used in western blot knockout validation on mouse samples at 1:20,000 (fig 2). Cell Stress Chaperones (2012) ncbi
Articles Reviewed
  1. Sowa A, Martin E, Martins I, Schmidt J, Depping R, Weber J, et al. Karyopherin ?-3 is a key protein in the pathogenesis of spinocerebellar ataxia type 3 controlling the nuclear localization of ataxin-3. Proc Natl Acad Sci U S A. 2018;115:E2624-E2633 pubmed publisher
  2. Ashkenazi A, Bento C, Ricketts T, Vicinanza M, Siddiqi F, Pavel M, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017;545:108-111 pubmed publisher
  3. Tu Y, Liu H, Zhu X, Shen H, Ma X, Wang F, et al. Ataxin-3 promotes genome integrity by stabilizing Chk1. Nucleic Acids Res. 2017;45:4532-4549 pubmed publisher
  4. Hansen S, Stummann T, Borland H, Hasholt L, Tumer Z, Nielsen J, et al. Induced pluripotent stem cell - derived neurons for the study of spinocerebellar ataxia type 3. Stem Cell Res. 2016;17:306-317 pubmed publisher
  5. Hansen S, Borland H, Hasholt L, Tumer Z, Nielsen J, Rasmussen M, et al. Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.B11. Stem Cell Res. 2016;16:589-92 pubmed publisher
  6. Hansen S, Borland H, Hasholt L, Tumer Z, Nielsen J, Rasmussen M, et al. Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11. Stem Cell Res. 2016;16:553-6 pubmed publisher
  7. Matos C, Nóbrega C, Louros S, Almeida B, Ferreiro E, Valero J, et al. Ataxin-3 phosphorylation decreases neuronal defects in spinocerebellar ataxia type 3 models. J Cell Biol. 2016;212:465-80 pubmed publisher
  8. Zeng L, Wang B, Merillat S, N Minakawa E, Perkins M, Ramani B, et al. Differential recruitment of UBQLN2 to nuclear inclusions in the polyglutamine diseases HD and SCA3. Neurobiol Dis. 2015;82:281-288 pubmed publisher
  9. Almeida B, Abreu I, Matos C, Fraga J, Fernandes S, Macedo M, et al. SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97. Biochim Biophys Acta. 2015;1852:1950-9 pubmed publisher
  10. Yao Y, Cui X, Al Ramahi I, Sun X, Li B, Hou J, et al. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. elife. 2015;4: pubmed publisher
  11. Gao R, Liu Y, Silva Fernandes A, Fang X, Paulucci Holthauzen A, Chatterjee A, et al. Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3. PLoS Genet. 2015;11:e1004834 pubmed publisher
  12. Liman J, Deeg S, Voigt A, Vo feldt H, Dohm C, Karch A, et al. CDK5 protects from caspase-induced Ataxin-3 cleavage and neurodegeneration. J Neurochem. 2014;129:1013-23 pubmed publisher
  13. Aiba Y, Hu J, Liu J, Xiang Q, Martinez C, Corey D. Allele-selective inhibition of expression of huntingtin and ataxin-3 by RNA duplexes containing unlocked nucleic acid substitutions. Biochemistry. 2013;52:9329-38 pubmed publisher
  14. Liu J, Yu D, Aiba Y, Pendergraff H, Swayze E, Lima W, et al. ss-siRNAs allele selectively inhibit ataxin-3 expression: multiple mechanisms for an alternative gene silencing strategy. Nucleic Acids Res. 2013;41:9570-83 pubmed publisher
  15. Liu J, Pendergraff H, Narayanannair K, Lackey J, Kuchimanchi S, Rajeev K, et al. RNA duplexes with abasic substitutions are potent and allele-selective inhibitors of huntingtin and ataxin-3 expression. Nucleic Acids Res. 2013;41:8788-801 pubmed publisher
  16. Nguyen H, Hübener J, Weber J, Grueninger S, Riess O, Weiss A. Cerebellar soluble mutant ataxin-3 level decreases during disease progression in Spinocerebellar Ataxia Type 3 mice. PLoS ONE. 2013;8:e62043 pubmed publisher
  17. Hübener J, Weber J, Richter C, Honold L, Weiss A, Murad F, et al. Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3). Hum Mol Genet. 2013;22:508-18 pubmed publisher
  18. Reina C, Nabet B, Young P, Pittman R. Basal and stress-induced Hsp70 are modulated by ataxin-3. Cell Stress Chaperones. 2012;17:729-42 pubmed publisher