This is a Validated Antibody Database (VAD) review about mouse Atp2a1, based on 24 published articles (read how Labome selects the articles), using Atp2a1 antibody in all methods. It is aimed to help Labome visitors find the most suited Atp2a1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atp2a1 synonym: SERCA1; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SR Ca(2+)-ATPase 1; calcium pump 1; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; endoplasmic reticulum class 1/2 Ca(2+) ATPase

Invitrogen
mouse monoclonal (IIH11)
  • western blot; mouse; loading ...; fig 5a
In order to report the effects of short- and long-term high-fat diets on fast-twitch skeletal muscles, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-911) was used in western blot on mouse samples (fig 5a). Physiol Rep (2017) ncbi
mouse monoclonal (IIH11)
  • western blot; mouse; 1:1000; loading ...; fig s2f
In order to elucidate the mechanism by which the I4895T mutation in the type 1 ryanodine receptor/Ca(2+) release channel results in disease, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-911) was used in western blot on mouse samples at 1:1000 (fig s2f). Nat Commun (2017) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; loading ...; fig 2e
In order to assess the role of redox stress in PH-induced diaphragm weakness, Invitrogen Atp2a1 antibody (Thermo, MA3-911) was used in western blot on rat samples (fig 2e). PLoS ONE (2017) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; 1:5000; loading ...; fig 4a
In order to report the effects of eccentric contraction on calpain-dependent proteolysis of calcium ion-regulatory proteins and force production in fast-twitch skeletal muscles, Invitrogen Atp2a1 antibody (ThermoFisher Scientific, MA-911) was used in western blot on rat samples at 1:5000 (fig 4a). J Appl Physiol (1985) (2017) ncbi
mouse monoclonal (VE121G9)
  • western blot; mouse; 1:1000; fig 5a
In order to examine the effects of sildenafil on skeletal muscle, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on mouse samples at 1:1000 (fig 5a). Exp Mol Med (2016) ncbi
mouse monoclonal (VE121G9)
  • western blot; human; loading ...; fig 4
In order to assess the effect of increased and maintained frequency of speed endurance training on performance and muscular adaptations, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on human samples (fig 4). J Appl Physiol (1985) (2017) ncbi
mouse monoclonal (VE121G9)
  • western blot; mouse; fig 1
  • proximity ligation assay; human; 1:500; fig 5
  • immunohistochemistry - frozen section; human; 1:500; fig 3
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 2
In order to investigate the deficiency that affects SERCA expression and function in the skeletal muscle by Calpain 3, Invitrogen Atp2a1 antibody (Affinity BioReagents, MA3-912) was used in western blot on mouse samples (fig 1), in proximity ligation assay on human samples at 1:500 (fig 5), in immunohistochemistry - frozen section on human samples at 1:500 (fig 3), in immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig 2). Expert Rev Mol Med (2016) ncbi
mouse monoclonal (VE121G9)
  • western blot; zebrafish ; fig 2
In order to analyze Danio rerio for calsequestrins in cardiac and skeletal muscle, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on zebrafish samples (fig 2). J Muscle Res Cell Motil (2016) ncbi
mouse monoclonal (VE121G9)
  • western blot; human; fig 1
In order to assess how match performance parameters in trained footballers relate to skeletal muscle parameters, sprint ability, and intermittent exercise performance, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on human samples (fig 1). Eur J Appl Physiol (2016) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; 1:2000; fig 3
In order to study the effects of caloric restriction skeletal muscle contraction, thyroid hormone metabolism, and fiber composition upon refeeding, Invitrogen Atp2a1 antibody (Thermo Fisher Scientific, MA3-911) was used in western blot on rat samples at 1:2000 (fig 3). Front Physiol (2015) ncbi
mouse monoclonal (IIH11)
  • western blot; rabbit
In order to test if sAnk1 and SERCA1 interact, Invitrogen Atp2a1 antibody (Thermo Scientific, IIH11) was used in western blot on rabbit samples . J Biol Chem (2015) ncbi
mouse monoclonal (VE121G9)
  • immunohistochemistry - frozen section; human; 1:2500; fig 2
  • western blot; human; 1:2500; fig 1b
In order to investigate Sarco/endoplasmic reticulum Ca(2+)-ATPase 1 splice variants, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in immunohistochemistry - frozen section on human samples at 1:2500 (fig 2) and in western blot on human samples at 1:2500 (fig 1b). Biochim Biophys Acta (2015) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; fig 1
In order to explore the mechanisms of arthritis-induced muscle dysfunction in rats with adjuvant-induced arthritis, Invitrogen Atp2a1 antibody (Thermo Fisher Scientific, MA3-911) was used in western blot on rat samples (fig 1). Skelet Muscle (2015) ncbi
mouse monoclonal (VE121G9)
  • western blot; human; fig 3g
In order to study mechanisms by which chronic beta2-adrenergic stimulation enhances muscle force and power output during maximal cycle ergometer exercise in young men, Invitrogen Atp2a1 antibody (Thermo Fisher Scientific,, MA3-912) was used in western blot on human samples (fig 3g). J Appl Physiol (1985) (2015) ncbi
mouse monoclonal (VE121G9)
  • western blot; human; 1:10,000; fig 3
In order to report the importance of Reelin in protecting the brain against Abeta-induced synaptic dysfunction and memory impairment, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on human samples at 1:10,000 (fig 3). Sci Signal (2015) ncbi
mouse monoclonal (VE121G9)
  • western blot; human; 1:10,000; fig 3
In order to study the expression of oxidative enzymes in fast-twitch muscle fibers after various exercise regimens, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on human samples at 1:10,000 (fig 3). Physiol Rep (2015) ncbi
mouse monoclonal (IIH11)
  • western blot; rat
In order to examine calcium-related proteins in the intrinsic laryngeal muscles, cricothyroid, and tibialis anterior muscles from male Sprague-Dawley rats, Invitrogen Atp2a1 antibody (Affinity Bioreagents, MA3-911) was used in western blot on rat samples . Physiol Rep (2015) ncbi
mouse monoclonal (VE121G9)
  • western blot; mouse; 1:1000; fig 1b
In order to study the role of SERCA1b during skeletal muscle differentiation, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-912) was used in western blot on mouse samples at 1:1000 (fig 1b). PLoS ONE (2015) ncbi
mouse monoclonal (IIH11)
  • western blot; mouse
In order to investigate Mitsugumin 56 in skeletal muscle maturation using knockout mice, Invitrogen Atp2a1 antibody (Thermo Scientific, MA3-911) was used in western blot on mouse samples . FEBS Lett (2015) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; 1:1000; fig 2
In order to study acute in vivo contraction in rat hindlimb and the higher level of PLIN5 and not PLIN3 in isolated skeletal muscle mitochondria, Invitrogen Atp2a1 antibody (Thermoscientific, MA3-911) was used in western blot on rat samples at 1:1000 (fig 2). Physiol Rep (2014) ncbi
mouse monoclonal (VE121G9)
  • immunohistochemistry - frozen section; human; fig 3
In order to analyze paraspinal muscles in neuromuscularly healthy subjects, Invitrogen Atp2a1 antibody (Thermo Scientific, VE121G9) was used in immunohistochemistry - frozen section on human samples (fig 3). Muscle Nerve (2015) ncbi
mouse monoclonal (VE121G9)
  • western blot; human
In order to study the effects of speed endurance training and heavy resistance training on running performance improvement, Invitrogen Atp2a1 antibody (Thermo Fischer Scientific, MA3-912) was used in western blot on human samples . J Appl Physiol (1985) (2014) ncbi
mouse monoclonal (IIH11)
  • western blot; rat; 1:2500
In order to examine the effects of spinal cord transection on sarco(endo)plasmic reticulum calcium ATPase pump isoform protein levels in the slow rat soleus, Invitrogen Atp2a1 antibody (Affinity Bioreagents, IIH11) was used in western blot on rat samples at 1:2500. Biochim Biophys Acta (2007) ncbi
Cell Signaling Technology
rabbit monoclonal (D54G12)
  • western blot; human; 1:1000; loading ...; fig 1b
In order to determine the role of calcium regulation in sporadic inclusion body myositis, Cell Signaling Technology Atp2a1 antibody (Cell Signaling, 12293s) was used in western blot on human samples at 1:1000 (fig 1b). Acta Neuropathol Commun (2017) ncbi
Articles Reviewed
  1. Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
  2. Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  3. Amici D, Pinal Fernández I, Mázala D, Lloyd T, Corse A, Christopher Stine L, et al. Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis. Acta Neuropathol Commun. 2017;5:24 pubmed publisher
  4. Himori K, Abe M, Tatebayashi D, Lee J, Westerblad H, Lanner J, et al. Superoxide dismutase/catalase mimetic EUK-134 prevents diaphragm muscle weakness in monocrotalin-induced pulmonary hypertension. PLoS ONE. 2017;12:e0169146 pubmed publisher
  5. Kanzaki K, Watanabe D, Kuratani M, Yamada T, Matsunaga S, Wada M. Role of calpain in eccentric contraction-induced proteolysis of Ca2+-regulatory proteins and force depression in rat fast-twitch skeletal muscle. J Appl Physiol (1985). 2017;122:396-405 pubmed publisher
  6. Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
  7. Skovgaard C, Almquist N, Bangsbo J. Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners. J Appl Physiol (1985). 2017;122:48-59 pubmed publisher
  8. Toral Ojeda I, Aldanondo G, Lasa Elgarresta J, Lasa Fernández H, Fernandez Torron R, Lopez de Munain A, et al. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert Rev Mol Med. 2016;18:e7 pubmed publisher
  9. Furlan S, Mosole S, Murgia M, Nagaraj N, Argenton F, Volpe P, et al. Calsequestrins in skeletal and cardiac muscle from adult Danio rerio. J Muscle Res Cell Motil. 2016;37:27-39 pubmed publisher
  10. Mohr M, Thomassen M, Girard O, Racinais S, Nybo L. Muscle variables of importance for physiological performance in competitive football. Eur J Appl Physiol. 2016;116:251-62 pubmed publisher
  11. de Andrade P, Neff L, Strosova M, Arsenijevic D, Patthey Vuadens O, Scapozza L, et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol. 2015;6:254 pubmed publisher
  12. Desmond P, Muriel J, Markwardt M, Rizzo M, Bloch R. Identification of Small Ankyrin 1 as a Novel Sarco(endo)plasmic Reticulum Ca2+-ATPase 1 (SERCA1) Regulatory Protein in Skeletal Muscle. J Biol Chem. 2015;290:27854-67 pubmed publisher
  13. Zhao Y, Ogawa H, Yonekura S, Mitsuhashi H, Mitsuhashi S, Nishino I, et al. Functional analysis of SERCA1b, a highly expressed SERCA1 variant in myotonic dystrophy type 1 muscle. Biochim Biophys Acta. 2015;1852:2042-7 pubmed publisher
  14. Yamada T, Abe M, Lee J, Tatebayashi D, Himori K, Kanzaki K, et al. Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment. Skelet Muscle. 2015;5:20 pubmed publisher
  15. Hostrup M, Kalsen A, Onslev J, Jessen S, Haase C, Habib S, et al. Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men. J Appl Physiol (1985). 2015;119:475-86 pubmed publisher
  16. Lane Donovan C, Philips G, Wasser C, Durakoglugil M, Masiulis I, Upadhaya A, et al. Reelin protects against amyloid β toxicity in vivo. Sci Signal. 2015;8:ra67 pubmed publisher
  17. Christensen P, Gunnarsson T, Thomassen M, Wilkerson D, Nielsen J, Bangsbo J. Unchanged content of oxidative enzymes in fast-twitch muscle fibers and V˙O2 kinetics after intensified training in trained cyclists. Physiol Rep. 2015;3: pubmed publisher
  18. Ferretti R, Marques M, Khurana T, Santo Neto H. Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles. Physiol Rep. 2015;3: pubmed publisher
  19. Tóth A, Fodor J, Vincze J, Oláh T, Juhász T, Zákány R, et al. The Effect of SERCA1b Silencing on the Differentiation and Calcium Homeostasis of C2C12 Skeletal Muscle Cells. PLoS ONE. 2015;10:e0123583 pubmed publisher
  20. Van B, Nishi M, Komazaki S, Ichimura A, Kakizawa S, Nakanaga K, et al. Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. FEBS Lett. 2015;589:1095-104 pubmed publisher
  21. Ramos S, Macpherson R, Turnbull P, Bott K, LeBlanc P, Ward W, et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol Rep. 2014;2: pubmed publisher
  22. Zimmermann C, Kalepu R, Ponfick M, Reichel H, Cakir B, Zierz S, et al. Histological characterization and biochemical analysis of paraspinal muscles in neuromuscularly healthy subjects. Muscle Nerve. 2015;52:45-54 pubmed publisher
  23. Skovgaard C, Christensen P, Larsen S, Andersen T, Thomassen M, Bangsbo J. Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners. J Appl Physiol (1985). 2014;117:1097-109 pubmed publisher
  24. Talmadge R, Paalani M. Sarco(endo)plasmic reticulum calcium pump isoforms in paralyzed rat slow muscle. Biochim Biophys Acta. 2007;1770:1187-93 pubmed