This is a Validated Antibody Database (VAD) review about mouse Anpep, based on 20 published articles (read how Labome selects the articles), using Anpep antibody in all methods. It is aimed to help Labome visitors find the most suited Anpep antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Anpep synonym: AP-M; AP-N; Apn; Cd13; P150

Knockout validation
Abcam
domestic rabbit monoclonal (EPR4058)
  • immunohistochemistry knockout validation; pigs ; loading ...; fig 1f
  • western blot knockout validation; pigs ; 1:300; loading ...; fig 1g
Abcam Anpep antibody (Abcam, ab108310) was used in immunohistochemistry knockout validation on pigs samples (fig 1f) and in western blot knockout validation on pigs samples at 1:300 (fig 1g). elife (2020) ncbi
Abcam
domestic rabbit monoclonal (EPR4058)
  • immunocytochemistry; human; 1:100; fig 1h
Abcam Anpep antibody (abcam, ab108310) was used in immunocytochemistry on human samples at 1:100 (fig 1h). Stem Cell Res Ther (2021) ncbi
domestic rabbit monoclonal (EPR4058)
  • western blot; human; 1:500; loading ...; fig 6e
Abcam Anpep antibody (Abcam, ab108310) was used in western blot on human samples at 1:500 (fig 6e). Adv Sci (Weinh) (2020) ncbi
domestic rabbit monoclonal (EPR4058)
  • immunohistochemistry knockout validation; pigs ; loading ...; fig 1f
  • western blot knockout validation; pigs ; 1:300; loading ...; fig 1g
Abcam Anpep antibody (Abcam, ab108310) was used in immunohistochemistry knockout validation on pigs samples (fig 1f) and in western blot knockout validation on pigs samples at 1:300 (fig 1g). elife (2020) ncbi
Bio-Rad
rat monoclonal (R3-63)
  • immunohistochemistry; mouse; 1:100; loading ...; fig s2d
Bio-Rad Anpep antibody (AbD Serotec, MCA2183GA) was used in immunohistochemistry on mouse samples at 1:100 (fig s2d). Nat Commun (2019) ncbi
rat monoclonal (R3-63)
  • immunohistochemistry - free floating section; mouse; 1:300; loading ...; fig 2a
In order to describe a role for sphingosine 1-phosphate receptor-1 in regulating blood brain barrier permeability, Bio-Rad Anpep antibody (Serotec, MCA2183EL) was used in immunohistochemistry - free floating section on mouse samples at 1:300 (fig 2a). Proc Natl Acad Sci U S A (2017) ncbi
rat monoclonal (R3-63)
  • immunohistochemistry - frozen section; mouse; 1:100; fig S1
Bio-Rad Anpep antibody (AbD Serotec, MCA2183GA) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig S1). Nat Commun (2016) ncbi
rat monoclonal (R3-63)
  • immunohistochemistry; mouse; 1:400; fig 5
Bio-Rad Anpep antibody (AbD Serotec, MCA2183GA) was used in immunohistochemistry on mouse samples at 1:400 (fig 5). EMBO Mol Med (2016) ncbi
rat monoclonal (R3-63)
  • immunohistochemistry - free floating section; mouse; fig 3
In order to characterize the rearrangement in the cerebroventricular malformation and cerebrovascular tree due to a tPA deficiency in mice, Bio-Rad Anpep antibody (AbD Serotec, MCA2183) was used in immunohistochemistry - free floating section on mouse samples (fig 3). Front Cell Neurosci (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (12)
  • immunohistochemistry - paraffin section; human; loading ...; fig 6
Santa Cruz Biotechnology Anpep antibody (Santa Cruz Biotechnology Inc, sc-136484) was used in immunohistochemistry - paraffin section on human samples (fig 6). Oncogene (2018) ncbi
mouse monoclonal (WM15)
  • flow cytometry; mouse; 1:20; loading ...; fig 6c
Santa Cruz Biotechnology Anpep antibody (Santa Cruz, sc-51522) was used in flow cytometry on mouse samples at 1:20 (fig 6c). J Cell Mol Med (2014) ncbi
mouse monoclonal (3D8)
  • immunohistochemistry; mouse
Santa Cruz Biotechnology Anpep antibody (Santa Cruz Biotechnology, sc-13536) was used in immunohistochemistry on mouse samples . Am J Pathol (2014) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - free floating section; mouse; 1:200; loading ...; fig 4b, 5b
R&D Systems Anpep antibody (R&D systems, AF2335) was used in immunohistochemistry - free floating section on mouse samples at 1:200 (fig 4b, 5b). Nat Commun (2022) ncbi
domestic goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4a
R&D Systems Anpep antibody (R&D System, AF2335) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 4a). Proc Natl Acad Sci U S A (2022) ncbi
domestic goat polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1g, s2a
R&D Systems Anpep antibody (R&D Systems, AF2335) was used in immunohistochemistry on mouse samples at 1:100 (fig 1g, s2a). J Exp Med (2021) ncbi
BD Biosciences
rat monoclonal (R3-242)
  • immunohistochemistry; mouse; 1:200; loading ...
BD Biosciences Anpep antibody (BD Pharmingen, 558744) was used in immunohistochemistry on mouse samples at 1:200. Nat Commun (2020) ncbi
rat monoclonal (R3-242)
  • flow cytometry; mouse; 1:100; loading ...; fig s6c
BD Biosciences Anpep antibody (BD, 558745) was used in flow cytometry on mouse samples at 1:100 (fig s6c). Nat Commun (2019) ncbi
rat monoclonal (R3-242)
  • flow cytometry; human; fig 3
BD Biosciences Anpep antibody (BD Biosciences, 558744) was used in flow cytometry on human samples (fig 3). Stem Cell Rev (2015) ncbi
rat monoclonal (R3-242)
  • flow cytometry; mouse
BD Biosciences Anpep antibody (BD, R3-242) was used in flow cytometry on mouse samples . Nature (2015) ncbi
rat monoclonal (R3-242)
  • immunohistochemistry; mouse; fig 3
In order to study the blood-brain barrier function in germ free mice, BD Biosciences Anpep antibody (BD Pharmingen, 558744) was used in immunohistochemistry on mouse samples (fig 3). Sci Transl Med (2014) ncbi
rat monoclonal (R3-242)
  • immunohistochemistry - frozen section; mouse; 1:100
In order to identify mechanisms governing the establishment of a functional blood brain barrier, BD Biosciences Anpep antibody (BD Pharmingen, 558744) was used in immunohistochemistry - frozen section on mouse samples at 1:100. Nature (2014) ncbi
rat monoclonal (R3-242)
  • immunohistochemistry; mouse; 1:200
In order to identify neurogenic capacity in adult murine hypothalamus, BD Biosciences Anpep antibody (BD Pharminogen, 558744) was used in immunohistochemistry on mouse samples at 1:200. PLoS ONE (2013) ncbi
Articles Reviewed
  1. O Shea T, Ao Y, Wang S, Wollenberg A, Kim J, Ramos Espinoza R, et al. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice. Nat Commun. 2022;13:5702 pubmed publisher
  2. Zhao Q, Dai W, Chen H, Jacobs R, Zlokovic B, Lund B, et al. Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation. Proc Natl Acad Sci U S A. 2022;119:e2113310119 pubmed publisher
  3. Yokomizo R, Fujiki Y, Kishigami H, Kishi H, Kiyono T, Nakayama S, et al. Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder. Stem Cell Res Ther. 2021;12:130 pubmed publisher
  4. Nikolakopoulou A, Wang Y, Ma Q, Sagare A, Montagne A, Huuskonen M, et al. Endothelial LRP1 protects against neurodegeneration by blocking cyclophilin A. J Exp Med. 2021;218: pubmed publisher
  5. Wu A, Sung Y, Chen Y, Chou S, Guo V, Chien J, et al. Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism. Adv Sci (Weinh). 2020;7:2001467 pubmed publisher
  6. Xu K, Zhou Y, Mu Y, Liu Z, Hou S, Xiong Y, et al. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance. elife. 2020;9: pubmed publisher
  7. Kang L, Yu H, Yang X, Zhu Y, Bai X, Wang R, et al. Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke. Nat Commun. 2020;11:2488 pubmed publisher
  8. Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  9. Seidi K, Jahanban Esfahlan R, Monhemi H, Zare P, Minofar B, Daei Farshchi Adli A, et al. NGR (Asn-Gly-Arg)-targeted delivery of coagulase to tumor vasculature arrests cancer cell growth. Oncogene. 2018;37:3967-3980 pubmed publisher
  10. Yanagida K, Liu C, Faraco G, Galvani S, Smith H, Burg N, et al. Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1. Proc Natl Acad Sci U S A. 2017;114:4531-4536 pubmed publisher
  11. Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
  12. Körbelin J, Dogbevia G, Michelfelder S, Ridder D, Hunger A, Wenzel J, et al. A brain microvasculature endothelial cell-specific viral vector with the potential to treat neurovascular and neurological diseases. EMBO Mol Med. 2016;8:609-25 pubmed publisher
  13. Stefanitsch C, Lawrence A, Olverling A, Nilsson I, Fredriksson L. tPA Deficiency in Mice Leads to Rearrangement in the Cerebrovascular Tree and Cerebroventricular Malformations. Front Cell Neurosci. 2015;9:456 pubmed publisher
  14. Zhou H, Martínez H, Sun B, Li A, Zimmer M, Katsanis N, et al. Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. Stem Cell Rev. 2015;11:652-65 pubmed publisher
  15. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  16. Braniste V, Al Asmakh M, Kowal C, Anuar F, Abbaspour A, Tóth M, et al. The gut microbiota influences blood-brain barrier permeability in mice. Sci Transl Med. 2014;6:263ra158 pubmed publisher
  17. Ben Zvi A, Lacoste B, Kur E, Andreone B, Mayshar Y, Yan H, et al. Mfsd2a is critical for the formation and function of the blood-brain barrier. Nature. 2014;509:507-11 pubmed publisher
  18. Schuster A, Klotz M, Schwab T, Di Liddo R, Bertalot T, Schrenk S, et al. Maintenance of the enteric stem cell niche by bacterial lipopolysaccharides? Evidence and perspectives. J Cell Mol Med. 2014;18:1429-43 pubmed publisher
  19. Li J, Tan H, Wang X, Li Y, Samuelson L, Li X, et al. Circulating fibrocytes stabilize blood vessels during angiogenesis in a paracrine manner. Am J Pathol. 2014;184:556-71 pubmed publisher
  20. Robins S, Trudel E, Rotondi O, Liu X, Djogo T, Kryzskaya D, et al. Evidence for NG2-glia derived, adult-born functional neurons in the hypothalamus. PLoS ONE. 2013;8:e78236 pubmed publisher