This is a Validated Antibody Database (VAD) review about mouse Aire, based on 7 published articles (read how Labome selects the articles), using Aire antibody in all methods. It is aimed to help Labome visitors find the most suited Aire antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Invitrogen
rat monoclonal (5H12)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3s2a
  • flow cytometry; mouse; 1:200; loading ...; fig 3c
Invitrogen Aire antibody (eBioscience, 53-5934-82) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 3s2a) and in flow cytometry on mouse samples at 1:200 (fig 3c). elife (2022) ncbi
rat monoclonal (5H12)
  • flow cytometry; mouse; loading ...; fig 2c
Invitrogen Aire antibody (eBioscience, 5H12) was used in flow cytometry on mouse samples (fig 2c). elife (2020) ncbi
rat monoclonal (5H12)
  • immunohistochemistry; mouse; loading ...; fig 6g
In order to explore the contribution of epithelial cells to systemic sclerosis pathogenesis, Invitrogen Aire antibody (eBioscience, 14-5934) was used in immunohistochemistry on mouse samples (fig 6g). J Exp Med (2017) ncbi
rat monoclonal (5H12)
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 1d
In order to explore how AIRE regulates medullary thymic epithelial cell development, Invitrogen Aire antibody (eBioscience, 5H12) was used in immunohistochemistry - frozen section on mouse samples at 1:300 (fig 1d). J Exp Med (2016) ncbi
rat monoclonal (5H12)
  • immunocytochemistry; mouse; fig 1
In order to assess mediation by androgen control of autoimmune regulator due to sex bias in CNS autoimmune disease, Invitrogen Aire antibody (eBioscience, 5H12) was used in immunocytochemistry on mouse samples (fig 1). Nat Commun (2016) ncbi
rat monoclonal (5H12)
  • flow cytometry; mouse; fig 6a
Invitrogen Aire antibody (eBioscience, 5H12) was used in flow cytometry on mouse samples (fig 6a). Sci Rep (2015) ncbi
rat monoclonal (5H12)
  • immunohistochemistry - frozen section; mouse; fig 4a
  • flow cytometry; mouse
  • immunocytochemistry; mouse; fig 1d
Invitrogen Aire antibody (ebioscience, 14-5934-82) was used in immunohistochemistry - frozen section on mouse samples (fig 4a), in flow cytometry on mouse samples and in immunocytochemistry on mouse samples (fig 1d). Nat Immunol (2014) ncbi
Articles Reviewed
  1. Lopes N, Boucherit N, Santamaria J, Provin N, Charaix J, Ferrier P, et al. Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages. elife. 2022;11: pubmed publisher
  2. Wells K, Miller C, Gschwind A, Wei W, Phipps J, Anderson M, et al. Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells. elife. 2020;9: pubmed publisher
  3. Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
  4. Akiyama N, Takizawa N, Miyauchi M, Yanai H, Tateishi R, Shinzawa M, et al. Identification of embryonic precursor cells that differentiate into thymic epithelial cells expressing autoimmune regulator. J Exp Med. 2016;213:1441-58 pubmed publisher
  5. Zhu M, Bakhru P, Conley B, Nelson J, Free M, Martin A, et al. Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator. Nat Commun. 2016;7:11350 pubmed publisher
  6. Sun L, Sun C, Liang Z, Li H, Chen L, Luo H, et al. FSP1(+) fibroblast subpopulation is essential for the maintenance and regeneration of medullary thymic epithelial cells. Sci Rep. 2015;5:14871 pubmed publisher
  7. Waterfield M, Khan I, Cortez J, Fan U, Metzger T, Greer A, et al. The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance. Nat Immunol. 2014;15:258-65 pubmed publisher