This is a Validated Antibody Database (VAD) review about mouse Afp, based on 31 published articles (read how Labome selects the articles), using Afp antibody in all methods. It is aimed to help Labome visitors find the most suited Afp antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Afp synonym: alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-foetoprotein

R&D Systems
mouse monoclonal (189502)
  • immunocytochemistry; human; fig 3f
R&D Systems Afp antibody (R&D Systems, MAB1368) was used in immunocytochemistry on human samples (fig 3f). Biol Open (2017) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; human; 1:250; loading ...; fig s1t
In order to show that a degenerative phenotype exhibiting mutant pendrin aggregates and increased susceptibility to cellular stresses in cochlear epithelial cells induced from patient-derived induced pluripotent stem cells, R&D Systems Afp antibody (R&D systems, MAB1368) was used in immunocytochemistry on human samples at 1:250 (fig s1t). Cell Rep (2017) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; human; loading ...; fig s1g
In order to demonstrate that iPS-derived cardiomyocytes with a heterozygous GATA4-G296S missense mutation show impaired contractility, calcium handling, and metabolic activity, R&D Systems Afp antibody (R&D, MAB1368) was used in immunocytochemistry on human samples (fig s1g). Cell (2016) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; mouse; 2500 ng/ml
In order to study how polycomb alters transcription and lineage priming, R&D Systems Afp antibody (R&D, MAB1368) was used in immunocytochemistry on mouse samples at 2500 ng/ml. elife (2016) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; mouse; 1:50; fig 1
R&D Systems Afp antibody (R&D Systems, MAB1368) was used in immunocytochemistry on mouse samples at 1:50 (fig 1). Stem Cell Reports (2015) ncbi
mouse monoclonal (189502)
  • immunohistochemistry; cow; 1:100; fig 6
In order to analyze PiggyBac transposition of doxycycline-inducible transcription factors due to generation of niave bovine induced pluripotent stem cells, R&D Systems Afp antibody (R&D Systems, MAB1368) was used in immunohistochemistry on cow samples at 1:100 (fig 6). PLoS ONE (2015) ncbi
mouse monoclonal (189502)
  • immunohistochemistry; human; 1:100; fig 3
R&D Systems Afp antibody (R&D, MAB1368) was used in immunohistochemistry on human samples at 1:100 (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (189502)
  • flow cytometry; mouse; 1:200
R&D Systems Afp antibody (R&D, MAB1368) was used in flow cytometry on mouse samples at 1:200. Springerplus (2014) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; human; 1:200
R&D Systems Afp antibody (R & D Systems, MAB1368) was used in immunocytochemistry on human samples at 1:200. Acta Naturae (2014) ncbi
mouse monoclonal (189502)
  • immunocytochemistry; mouse; fig 2
R&D Systems Afp antibody (R&D Systems, MAB1368) was used in immunocytochemistry on mouse samples (fig 2). PLoS ONE (2013) ncbi
Abcam
mouse monoclonal (AFP-01)
  • immunocytochemistry; human; 1:100; loading ...; fig 1g
In order to report the first model system to investigate the pathomechanism underlying GTP cyclohydrolase 1 mutations, Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples at 1:100 (fig 1g). Stem Cell Res (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 5f
  • western blot; mouse; 1:2000; loading ...; fig s4d
Abcam Afp antibody (Abcam, ab46799) was used in western blot on human samples at 1:2000 (fig 5f) and in western blot on mouse samples at 1:2000 (fig s4d). Cell Rep (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4d
Abcam Afp antibody (Abcam, 46799) was used in western blot on mouse samples at 1:1000 (fig 4d). Nat Commun (2016) ncbi
mouse monoclonal (AFP-01)
  • immunocytochemistry; human; 1:100; loading ...; fig 1f
In order to generate and characterize tyrosine hydroxylase-expressing iPSCs, Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples at 1:100 (fig 1f). Stem Cell Res (2016) ncbi
mouse monoclonal (AFP-01)
  • immunocytochemistry; human; 1:200; fig s2
Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples at 1:200 (fig s2). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig s5e
Abcam Afp antibody (Abcam, ab46799) was used in immunocytochemistry on mouse samples at 1:100 (fig s5e). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3b
Abcam Afp antibody (Abcam, ab46799) was used in immunohistochemistry - paraffin section on mouse samples (fig 3b). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 1n-o
  • western blot; mouse; loading ...; fig 1p
Abcam Afp antibody (Abcam, ab46799) was used in immunohistochemistry on mouse samples (fig 1n-o) and in western blot on mouse samples (fig 1p). Oncotarget (2016) ncbi
mouse monoclonal (AFP-01)
  • immunocytochemistry; human; 5 ug/ml; fig s3c
Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples at 5 ug/ml (fig s3c). Cell Death Dis (2015) ncbi
mouse monoclonal (AFP-01)
  • immunocytochemistry; human; fig 5a
Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples (fig 5a). Sci Rep (2014) ncbi
mouse monoclonal (AFP-01)
  • immunocytochemistry; human
Abcam Afp antibody (Abcam, ab3980) was used in immunocytochemistry on human samples . PLoS ONE (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C3)
  • western blot; human; loading ...; fig 3d
Santa Cruz Biotechnology Afp antibody (Santa, sc-8399) was used in western blot on human samples (fig 3d). Carcinogenesis (2017) ncbi
mouse monoclonal (C3)
  • immunocytochemistry; human; fig 4
Santa Cruz Biotechnology Afp antibody (Santa Cruz, sc-8399) was used in immunocytochemistry on human samples (fig 4). Stem Cells Int (2016) ncbi
mouse monoclonal (C3)
  • immunocytochemistry; human; 1:200; fig s8
Santa Cruz Biotechnology Afp antibody (Santa Cruz, sc-8399) was used in immunocytochemistry on human samples at 1:200 (fig s8). Nat Commun (2016) ncbi
mouse monoclonal (C3)
  • immunohistochemistry - paraffin section; human; fig 1
Santa Cruz Biotechnology Afp antibody (Santa Cruz, sc-8399) was used in immunohistochemistry - paraffin section on human samples (fig 1). Oncotarget (2015) ncbi
mouse monoclonal (C3)
  • immunocytochemistry; human; 1:100
In order to study two pluripotent stem cell populations in regards to a neural precurson cell population, Santa Cruz Biotechnology Afp antibody (Santa Cruz, sc-8399) was used in immunocytochemistry on human samples at 1:100. Stem Cell Res (2014) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1h
Proteintech Group Afp antibody (Proteintech, 14550-1-AP) was used in immunohistochemistry - paraffin section on mouse samples (fig 1h). Stem Cell Reports (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
In order to elucidate the origin of hepatocellular carcinoma, Proteintech Group Afp antibody (Proteintech, 14550-1-AP) was used in immunohistochemistry - paraffin section on mouse samples . J Clin Invest (2015) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; fig 7
In order to examine how the elasticity of the matrix affects the pluripotency of hPSCs, Invitrogen Afp antibody (Life Technology, PA5-21004) was used in immunocytochemistry on human samples (fig 7). Sci Rep (2015) ncbi
Cell Signaling Technology
mouse monoclonal (3H8)
  • immunocytochemistry; human; 1:200
In order to study age-related macular degeneration pathogenesis using stem cells differentiation into retinal pigment epithelial cells using T cells, Cell Signaling Technology Afp antibody (Cell signaling, 3903) was used in immunocytochemistry on human samples at 1:200. Front Aging Neurosci (2014) ncbi
mouse monoclonal (3H8)
  • immunocytochemistry; human
Cell Signaling Technology Afp antibody (Cell Signaling, 3903S) was used in immunocytochemistry on human samples . Biomaterials (2014) ncbi
Articles Reviewed
  1. Valanejad L, Lewis K, Wright M, Jiang Y, D Souza A, Karns R, et al. FXR-Gankyrin axis is involved in development of pediatric liver cancer. Carcinogenesis. 2017;38:738-747 pubmed publisher
  2. Jung Klawitter S, Ebersold J, Göhring G, Blau N, Opladen T. Generation of an iPSC line from a patient with GTP cyclohydrolase 1 (GCH1) deficiency: HDMC0061i-GCH1. Stem Cell Res. 2017;20:38-41 pubmed publisher
  3. Li G, Ji T, Chen J, Fu Y, Hou L, Feng Y, et al. CRL4DCAF8 Ubiquitin Ligase Targets Histone H3K79 and Promotes H3K9 Methylation in the Liver. Cell Rep. 2017;18:1499-1511 pubmed publisher
  4. Bharathan S, Manian K, Aalam S, Palani D, Deshpande P, Pratheesh M, et al. Systematic evaluation of markers used for the identification of human induced pluripotent stem cells. Biol Open. 2017;6:100-108 pubmed publisher
  5. Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, et al. Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 2017;18:68-81 pubmed publisher
  6. Jerić I, Maurer G, Cavallo A, Raguz J, Desideri E, Tarkowski B, et al. A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis. Nat Commun. 2016;7:13781 pubmed publisher
  7. Ang Y, Rivas R, Ribeiro A, Srivas R, Rivera J, Stone N, et al. Disease Model of GATA4 Mutation Reveals Transcription Factor Cooperativity in Human Cardiogenesis. Cell. 2016;167:1734-1749.e22 pubmed publisher
  8. Jung Klawitter S, Blau N, Sebe A, Ebersold J, Göhring G, Opladen T. Generation of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC. Stem Cell Res. 2016;17:580-583 pubmed publisher
  9. Okashita N, Suwa Y, Nishimura O, Sakashita N, Kadota M, Nagamatsu G, et al. PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency. Stem Cell Reports. 2016;7:1072-1086 pubmed publisher
  10. Illingworth R, Hölzenspies J, Roske F, Bickmore W, Brickman J. Polycomb enables primitive endoderm lineage priming in embryonic stem cells. elife. 2016;5: pubmed publisher
  11. Ahmadian Baghbaderani B, Tian X, Scotty Cadet J, Shah K, Walde A, Tran H, et al. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells. PLoS ONE. 2016;11:e0161229 pubmed publisher
  12. El Kehdy H, Pourcher G, Zhang W, Hamidouche Z, Goulinet Mainot S, Sokal E, et al. Hepatocytic Differentiation Potential of Human Fetal Liver Mesenchymal Stem Cells: In Vitro and In Vivo Evaluation. Stem Cells Int. 2016;2016:6323486 pubmed publisher
  13. Prieto J, León M, Ponsoda X, Sendra R, Bort R, Ferrer Lorente R, et al. Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming. Nat Commun. 2016;7:11124 pubmed publisher
  14. Ambade A, Satishchandran A, Szabo G. Alcoholic hepatitis accelerates early hepatobiliary cancer by increasing stemness and miR-122-mediated HIF-1α activation. Sci Rep. 2016;6:21340 pubmed publisher
  15. Wang Y, Cui R, Zhang X, Qiao Y, Liu X, Chang Y, et al. SIRT1 increases YAP- and MKK3-dependent p38 phosphorylation in mouse liver and human hepatocellular carcinoma. Oncotarget. 2016;7:11284-98 pubmed publisher
  16. Guye P, Ebrahimkhani M, Kipniss N, Velazquez J, Schoenfeld E, Kiani S, et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun. 2016;7:10243 pubmed publisher
  17. Higuchi A, Kao S, Ling Q, Chen Y, Li H, Alarfaj A, et al. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep. 2015;5:18136 pubmed publisher
  18. Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli A, Gambacurta A. Differentiation of human neuroblastoma cells toward the osteogenic lineage by mTOR inhibitor. Cell Death Dis. 2015;6:e1974 pubmed publisher
  19. Mu X, Español Suñer R, Mederacke I, Affò S, Manco R, Sempoux C, et al. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment. J Clin Invest. 2015;125:3891-903 pubmed publisher
  20. Neri T, Muggeo S, Paulis M, Caldana M, Crisafulli L, Strina D, et al. Targeted Gene Correction in Osteopetrotic-Induced Pluripotent Stem Cells for the Generation of Functional Osteoclasts. Stem Cell Reports. 2015;5:558-68 pubmed publisher
  21. Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, et al. Generation of Naïve Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors. PLoS ONE. 2015;10:e0135403 pubmed publisher
  22. Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, et al. Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes. PLoS ONE. 2015;10:e0129855 pubmed publisher
  23. Zhu M, Guo J, Li W, Lu Y, Fu S, Xie X, et al. Hepatitis B virus X protein induces expression of alpha-fetoprotein and activates PI3K/mTOR signaling pathway in liver cells. Oncotarget. 2015;6:12196-208 pubmed
  24. Pettinato G, Wen X, Zhang N. Formation of well-defined embryoid bodies from dissociated human induced pluripotent stem cells using microfabricated cell-repellent microwell arrays. Sci Rep. 2014;4:7402 pubmed publisher
  25. Pettinato G, Vanden Berg Foels W, Zhang N, Wen X. ROCK inhibitor is not required for embryoid body formation from singularized human embryonic stem cells. PLoS ONE. 2014;9:e100742 pubmed publisher
  26. Chang Y, Chang W, Hung K, Yang D, Cheng Y, Liao Y, et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci. 2014;6:191 pubmed publisher
  27. Petrakova O, Terskikh V, Chernioglo E, Ashapkin V, Bragin E, Shtratnikova V, et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus. 2014;3:183 pubmed publisher
  28. Muchkaeva I, Dashinimaev E, Artyuhov A, Myagkova E, Vorotelyak E, Yegorov Y, et al. Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells. Acta Naturae. 2014;6:45-53 pubmed
  29. Pryzhkova M, Aria I, Cheng Q, Harris G, Zan X, Gharib M, et al. Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate. Biomaterials. 2014;35:5098-109 pubmed publisher
  30. Mallon B, Hamilton R, Kozhich O, Johnson K, Fann Y, Rao M, et al. Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin. Stem Cell Res. 2014;12:376-86 pubmed publisher
  31. Okumura N, Akutsu H, Sugawara T, Miura T, Takezawa Y, Hosoda A, et al. ?-catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells. PLoS ONE. 2013;8:e63265 pubmed publisher