This is a Validated Antibody Database (VAD) review about mouse Acss2, based on 11 published articles (read how Labome selects the articles), using Acss2 antibody in all methods. It is aimed to help Labome visitors find the most suited Acss2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Acss2 synonym: 1110017C11Rik; ACAS; ACS; Acas1; Acas2; AceCS1; Acs1; aceCS

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D19C6)
  • immunohistochemistry - paraffin section; human; fig s6d
  • western blot; human; loading ...; fig 3a
  • western blot knockout validation; mouse; fig 4b
Cell Signaling Technology Acss2 antibody (Cell Signaling, 3658) was used in immunohistochemistry - paraffin section on human samples (fig s6d), in western blot on human samples (fig 3a) and in western blot knockout validation on mouse samples (fig 4b). Cancer Res (2021) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 6c
Abcam Acss2 antibody (Abcam, ab66038) was used in western blot on mouse samples (fig 6c). Science (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6a
  • western blot; mouse; loading ...; fig 6b
Abcam Acss2 antibody (Abcam, 66038) was used in immunohistochemistry - paraffin section on mouse samples (fig 6a) and in western blot on mouse samples (fig 6b). Genes Dev (2019) ncbi
domestic rabbit monoclonal (EPR8500)
  • immunohistochemistry; human; 1:50; fig 6e
  • western blot; human; fig 6a
Abcam Acss2 antibody (Abcam, ab133664) was used in immunohistochemistry on human samples at 1:50 (fig 6e) and in western blot on human samples (fig 6a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR8500)
  • immunocytochemistry; human; 1:150
Abcam Acss2 antibody (Epitomics, #6516?C1) was used in immunocytochemistry on human samples at 1:150. J Cell Physiol (2015) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5a
Invitrogen Acss2 antibody (Thermo Scientific, PA5-26612) was used in western blot on mouse samples (fig 5a). Cell Rep (2019) ncbi
domestic rabbit monoclonal (T.407.4)
  • western blot; mouse; loading ...; fig 1e
In order to investigate the involvement of acetyl-CoA synthetase in histone acetylation and hippocampal memory, Invitrogen Acss2 antibody (Thermo, MA5-145810) was used in western blot on mouse samples (fig 1e). Nature (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D19C6)
  • western blot; human; loading ...; fig 5f
Cell Signaling Technology Acss2 antibody (CST, 3658) was used in western blot on human samples (fig 5f). Cell Rep (2022) ncbi
domestic rabbit monoclonal (D19C6)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 9d
  • western blot; mouse; 1:1000; loading ...; fig 9c
Cell Signaling Technology Acss2 antibody (Cell Signaling, 3658) was used in immunohistochemistry - paraffin section on mouse samples (fig 9d) and in western blot on mouse samples at 1:1000 (fig 9c). J Biol Chem (2021) ncbi
domestic rabbit monoclonal (D19C6)
  • immunohistochemistry - paraffin section; human; fig s6d
  • western blot; human; loading ...; fig 3a
  • western blot knockout validation; mouse; fig 4b
Cell Signaling Technology Acss2 antibody (Cell Signaling, 3658) was used in immunohistochemistry - paraffin section on human samples (fig s6d), in western blot on human samples (fig 3a) and in western blot knockout validation on mouse samples (fig 4b). Cancer Res (2021) ncbi
domestic rabbit monoclonal (D19C6)
  • western blot; mouse; loading ...; fig 3i, s4f
Cell Signaling Technology Acss2 antibody (Cell Signaling, 3658) was used in western blot on mouse samples (fig 3i, s4f). Science (2019) ncbi
domestic rabbit monoclonal (D19C6)
  • ChIP-Seq; mouse; loading ...; fig 3a
  • western blot; mouse; loading ...; fig 1e
In order to investigate the involvement of acetyl-CoA synthetase in histone acetylation and hippocampal memory, Cell Signaling Technology Acss2 antibody (Cell Signaling, 3658) was used in ChIP-Seq on mouse samples (fig 3a) and in western blot on mouse samples (fig 1e). Nature (2017) ncbi
domestic rabbit monoclonal (D19C6)
  • immunohistochemistry - paraffin section; mouse
  • immunohistochemistry; mouse; 1:150; fig s3
In order to study stress erythropoiesis regulated by an acetate switch, Cell Signaling Technology Acss2 antibody (Cell Signaling Technology, 3658) was used in immunohistochemistry - paraffin section on mouse samples and in immunohistochemistry on mouse samples at 1:150 (fig s3). Nat Med (2014) ncbi
Articles Reviewed
  1. Pillai S, Mahmud I, Mahar R, Griffith C, Langsen M, Nguyen J, et al. Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy. Cell Rep. 2022;39:110796 pubmed publisher
  2. Nagati J, Kobeissy P, Nguyen M, Xu M, Garcia T, Comerford S, et al. Mammalian acetate-dependent acetyl CoA synthetase 2 contains multiple protein destabilization and masking elements. J Biol Chem. 2021;:101037 pubmed publisher
  3. Miller K, Pniewski K, Perry C, Papp S, Shaffer J, Velasco Silva J, et al. Targeting ACSS2 with a Transition-State Mimetic Inhibits Triple-Negative Breast Cancer Growth. Cancer Res. 2021;81:1252-1264 pubmed publisher
  4. Leone R, Zhao L, Englert J, Sun I, Oh M, Sun I, et al. Glutamine blockade induces divergent metabolic programs to overcome tumor immune evasion. Science. 2019;366:1013-1021 pubmed publisher
  5. Qiu J, Villa M, Sanin D, Buck M, O Sullivan D, Ching R, et al. Acetate Promotes T Cell Effector Function during Glucose Restriction. Cell Rep. 2019;27:2063-2074.e5 pubmed publisher
  6. Vodnala S, Eil R, Kishton R, Sukumar M, Yamamoto T, Ha N, et al. T cell stemness and dysfunction in tumors are triggered by a common mechanism. Science. 2019;363: pubmed publisher
  7. Shah A, Melhuish T, Fox T, Frierson H, Wotton D. TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth. Genes Dev. 2019;33:388-402 pubmed publisher
  8. Mews P, Donahue G, Drake A, Luczak V, Abel T, Berger S. Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Nature. 2017;546:381-386 pubmed publisher
  9. Gao X, Lin S, Ren F, Li J, Chen J, Yao C, et al. Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia. Nat Commun. 2016;7:11960 pubmed publisher
  10. Long P, Tighe S, Driscoll H, Fortner K, Viapiano M, Jaworski D. Acetate supplementation as a means of inducing glioblastoma stem-like cell growth arrest. J Cell Physiol. 2015;230:1929-43 pubmed publisher
  11. Xu M, Nagati J, Xie J, Li J, Walters H, Moon Y, et al. An acetate switch regulates stress erythropoiesis. Nat Med. 2014;20:1018-26 pubmed publisher