This is a Validated Antibody Database (VAD) review about human zyxin, based on 12 published articles (read how Labome selects the articles), using zyxin antibody in all methods. It is aimed to help Labome visitors find the most suited zyxin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
zyxin synonym: ESP-2; HED-2

Knockout validation
Abcam
domestic rabbit monoclonal (EPR4302)
  • western blot knockout validation; mouse; loading ...; fig 7a
  • immunohistochemistry; human; loading ...; fig 3e
  • western blot; human; loading ...; fig 1c
In order to evaluate the mechanisms of Weibel-Palade body exocytosis upon vascular injury, Abcam zyxin antibody (Abcam, ab109316) was used in western blot knockout validation on mouse samples (fig 7a), in immunohistochemistry on human samples (fig 3e) and in western blot on human samples (fig 1c). Nat Commun (2017) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig 4s2
Abcam zyxin antibody (Abcam, ab71842) was used in immunocytochemistry on human samples at 1:100 (fig 4s2). elife (2020) ncbi
domestic rabbit monoclonal (EPR4302)
  • western blot knockout validation; mouse; loading ...; fig 7a
  • immunohistochemistry; human; loading ...; fig 3e
  • western blot; human; loading ...; fig 1c
In order to evaluate the mechanisms of Weibel-Palade body exocytosis upon vascular injury, Abcam zyxin antibody (Abcam, ab109316) was used in western blot knockout validation on mouse samples (fig 7a), in immunohistochemistry on human samples (fig 3e) and in western blot on human samples (fig 1c). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...
In order to test if prolactin phosphorylates NEK3 to promote migration of breast cancer cells, Abcam zyxin antibody (Abcam, ab71842) was used in western blot on human samples at 1:1000. J Biol Chem (2016) ncbi
domestic rabbit monoclonal (EPR4302)
  • immunohistochemistry - paraffin section; human; 1:150; fig 7
  • immunoprecipitation; human; fig 4
  • immunocytochemistry; human; 1:200; fig 4
  • immunohistochemistry; human; 1:150; fig s4
  • western blot; human; 1:10,000; fig 1
Abcam zyxin antibody (Epitomics, 3586-1) was used in immunohistochemistry - paraffin section on human samples at 1:150 (fig 7), in immunoprecipitation on human samples (fig 4), in immunocytochemistry on human samples at 1:200 (fig 4), in immunohistochemistry on human samples at 1:150 (fig s4) and in western blot on human samples at 1:10,000 (fig 1). Nat Commun (2016) ncbi
  • immunocytochemistry; human; 1:200
  • western blot; human
In order to study the effect of Imatinib on VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells, Abcam zyxin antibody (Abcam, ab58210) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples . Biochim Biophys Acta (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • immunocytochemistry; human; 1:25; loading ...; fig 3a
Santa Cruz Biotechnology zyxin antibody (Santa Cruz Biotech, sc-293448) was used in immunocytochemistry on human samples at 1:25 (fig 3a). Am J Respir Cell Mol Biol (2019) ncbi
Synaptic Systems
mouse monoclonal (164D4)
  • immunocytochemistry; human; loading ...
In order to study the activation of alphavbeta3 integrin by ligand binding, Synaptic Systems zyxin antibody (Synaptic Systems, 307011) was used in immunocytochemistry on human samples . Cell Adh Migr (2016) ncbi
mouse monoclonal (164D4)
  • immunoprecipitation; human; fig s2
Synaptic Systems zyxin antibody (SYSY, 307011) was used in immunoprecipitation on human samples (fig s2). EMBO J (2015) ncbi
mouse monoclonal (164D4)
  • immunocytochemistry; mouse; 1:1000
In order to evaluate polyelectrolyte films for the study of myoblast cell adhesion and spatial organization, Synaptic Systems zyxin antibody (Synaptic System, 307011) was used in immunocytochemistry on mouse samples at 1:1000. Adv Funct Mater (2013) ncbi
Abnova
mouse monoclonal (2C10-4A7)
  • western blot; human; 1:500; fig s5
Abnova zyxin antibody (Abnova, H00007791-M01) was used in western blot on human samples at 1:500 (fig s5). J Cell Biol (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology zyxin antibody (Cell signaling, 3553) was used in western blot on human samples (fig 1). J Biol Chem (2016) ncbi
BD Biosciences
mouse monoclonal (21/Zyxin)
  • immunocytochemistry; human
In order to study the mechanism of the activation of focal adhesion protein vinculin by mechanical stress, BD Biosciences zyxin antibody (BD, 610521) was used in immunocytochemistry on human samples . Cancer Res (2014) ncbi
Articles Reviewed
  1. Butt B, Owen D, Jeffries C, Ivanova L, Hill C, Houghton J, et al. Insights into herpesvirus assembly from the structure of the pUL7:pUL51 complex. elife. 2020;9: pubmed publisher
  2. Gerlach B, Tubbesing K, Liao G, Rezey A, Wang R, Barroso M, et al. Phosphorylation of GMFγ by c-Abl Coordinates Lamellipodial and Focal Adhesion Dynamics to Regulate Airway Smooth Muscle Cell Migration. Am J Respir Cell Mol Biol. 2019;: pubmed publisher
  3. Han X, Li P, Yang Z, Huang X, Wei G, Sun Y, et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun. 2017;8:14639 pubmed publisher
  4. Harrington K, Clevenger C. Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem. 2016;291:21388-21406 pubmed
  5. Chen X, Stauffer S, Chen Y, Dong J. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. J Biol Chem. 2016;291:14761-72 pubmed publisher
  6. Ma B, Cheng H, Gao R, Mu C, Chen L, Wu S, et al. Zyxin-Siah2-Lats2 axis mediates cooperation between Hippo and TGF-β signalling pathways. Nat Commun. 2016;7:11123 pubmed publisher
  7. Schaufler V, Czichos Medda H, Hirschfeld Warnecken V, Neubauer S, Rechenmacher F, Medda R, et al. Selective binding and lateral clustering of ?5?1 and ?v?3 integrins: Unraveling the spatial requirements for cell spreading and focal adhesion assembly. Cell Adh Migr. 2016;10:505-515 pubmed
  8. Kenific C, Stehbens S, Goldsmith J, Leidal A, Faure N, Ye J, et al. NBR1 enables autophagy-dependent focal adhesion turnover. J Cell Biol. 2016;212:577-90 pubmed publisher
  9. Gill M, Turner R, Stevenson P, Way M. KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction. EMBO J. 2015;34:448-65 pubmed publisher
  10. Bernusso V, Machado Neto J, Pericole F, Vieira K, Duarte A, Traina F, et al. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. Biochim Biophys Acta. 2015;1853:388-95 pubmed publisher
  11. Rubashkin M, Cassereau L, Bainer R, DuFort C, Yui Y, Ou G, et al. Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate. Cancer Res. 2014;74:4597-611 pubmed publisher
  12. Monge C, Saha N, Boudou T, P zos V squez C, Dulong V, Glinel K, et al. Rigidity-patterned polyelectrolyte films to control myoblast cell adhesion and spatial organization. Adv Funct Mater. 2013;23:3432-3442 pubmed publisher