This is a Validated Antibody Database (VAD) review about human uPAR, based on 13 published articles (read how Labome selects the articles), using uPAR antibody in all methods. It is aimed to help Labome visitors find the most suited uPAR antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
uPAR synonym: CD87; U-PAR; UPAR; URKR

Abcam
domestic rabbit monoclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1f, 4a, s1d
Abcam uPAR antibody (Abcam, ab218106) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1f, 4a, s1d). Sci Adv (2022) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1e
Abcam uPAR antibody (Abcam, ab103791) was used in western blot on mouse samples (fig 1e). Cell Mol Life Sci (2018) ncbi
mouse monoclonal (R-4)
  • western blot; human; 1:1500; fig 4
Abcam uPAR antibody (Abcam, ab82220) was used in western blot on human samples at 1:1500 (fig 4). Mol Med Rep (2015) ncbi
Invitrogen
mouse monoclonal (VIM5)
  • flow cytometry; human; 1:200; loading ...; fig 7f
  • flow cytometry; mouse; 1:200; loading ...; fig 3c
In order to find that coagulation factor XII modulates immune responses, Invitrogen uPAR antibody (eBioscience, VIM5) was used in flow cytometry on human samples at 1:200 (fig 7f) and in flow cytometry on mouse samples at 1:200 (fig 3c). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100
In order to study relapsed small cell lung cancer by using patient-derived xenografts faithfully replicated clinical outcome in a phase II co-clinical trial of aresenic trioxide, Invitrogen uPAR antibody (Invitrogen, PA5-15478) was used in immunohistochemistry on human samples at 1:100. J Transl Med (2016) ncbi
BioLegend
mouse monoclonal (VIM5)
  • flow cytometry; human; 1:100; loading ...
BioLegend uPAR antibody (BioLegend, VIM5) was used in flow cytometry on human samples at 1:100. Nature (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (10G7)
  • western blot; human; loading ...; fig 1d
In order to determine the effects of resveratrol treatment on the senescence-associated secretory phenotype, Santa Cruz Biotechnology uPAR antibody (Santa Cruz, sc-13522) was used in western blot on human samples (fig 1d). J Gerontol A Biol Sci Med Sci (2017) ncbi
R&D Systems
domestic goat polyclonal
  • blocking or activating experiments; human; loading ...; fig s4c
  • immunoprecipitation; human; loading ...; fig s4a
  • western blot; human; loading ...; fig 2a
R&D Systems uPAR antibody (R&D Systems, AF807) was used in blocking or activating experiments on human samples (fig s4c), in immunoprecipitation on human samples (fig s4a) and in western blot on human samples (fig 2a). Cell Death Dis (2016) ncbi
Dako
mouse monoclonal (R4)
  • immunohistochemistry - paraffin section; dogs; 1:25; loading ...; fig 1a, 1g-r
Dako uPAR antibody (Dako, M7294) was used in immunohistochemistry - paraffin section on dogs samples at 1:25 (fig 1a, 1g-r). Onco Targets Ther (2017) ncbi
mouse monoclonal (R4)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1b
Dako uPAR antibody (Dako, R4) was used in immunohistochemistry - paraffin section on human samples (fig 1b). Cell Signal (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D7X2N)
  • western blot; human; loading ...; fig 8b
Cell Signaling Technology uPAR antibody (Cell Signaling, 12713S) was used in western blot on human samples (fig 8b). Int J Mol Med (2016) ncbi
BD Biosciences
mouse monoclonal (VIM5)
  • other; human; fig s1
In order to investigate the mechanisms of toxicity by the negative allosteric modulators of metabotropic glutamate receptor 5, BD Biosciences uPAR antibody (BD/Pharm, 555767) was used in other on human samples (fig s1). Cell Chem Biol (2017) ncbi
mouse monoclonal (VIM5)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences uPAR antibody (BD, 555768) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Articles Reviewed
  1. Qin L, Wang L, Zhang J, Zhou H, Yang Z, Wang Y, et al. Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer. Sci Adv. 2022;8:eabn3774 pubmed publisher
  2. Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, et al. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration. Nature. 2018;562:605-609 pubmed publisher
  3. Shang X, Shen C, Liu J, Tang L, Zhang H, Wang Y, et al. Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding. Cell Mol Life Sci. 2018;75:4371-4384 pubmed publisher
  4. Shah F, Stepan A, O Mahony A, Velichko S, Folias A, Houle C, et al. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators. Cell Chem Biol. 2017;24:858-869.e5 pubmed publisher
  5. Rossmeisl J, Hall Manning K, Robertson J, King J, Davalos R, Debinski W, et al. Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors. Onco Targets Ther. 2017;10:2077-2085 pubmed publisher
  6. Menicacci B, Laurenzana A, Chillà A, Margheri F, Peppicelli S, Tanganelli E, et al. Chronic Resveratrol Treatment Inhibits MRC5 Fibroblast SASP-Related Protumoral Effects on Melanoma Cells. J Gerontol A Biol Sci Med Sci. 2017;72:1187-1195 pubmed publisher
  7. Hau A, Leivo M, Gilder A, Hu J, Gonias S, Hansel D. mTORC2 activation is regulated by the urokinase receptor (uPAR) in bladder cancer. Cell Signal. 2017;29:96-106 pubmed publisher
  8. Narayanaswamy P, Tkachuk S, Haller H, Dumler I, Kiyan Y. CHK1 and RAD51 activation after DNA damage is regulated via urokinase receptor/TLR4 signaling. Cell Death Dis. 2016;7:e2383 pubmed publisher
  9. Li Y, Jiang D, Zhang Q, Liu X, Cai Z. Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4. Int J Mol Med. 2016;38:803-11 pubmed publisher
  10. Göbel K, Pankratz S, Asaridou C, Herrmann A, Bittner S, Merker M, et al. Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells. Nat Commun. 2016;7:11626 pubmed publisher
  11. Owonikoko T, Zhang G, Kim H, Stinson R, Bechara R, Zhang C, et al. Patient-derived xenografts faithfully replicated clinical outcome in a phase II co-clinical trial of arsenic trioxide in relapsed small cell lung cancer. J Transl Med. 2016;14:111 pubmed publisher
  12. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  13. Huang W, Li L, Tian X, Yan J, Yang X, Wang X, et al. Astragalus and Paeoniae Radix Rubra extract (APE) inhibits hepatic stellate cell activation by modulating transforming growth factor-β/Smad pathway. Mol Med Rep. 2015;11:2569-77 pubmed publisher