This is a Validated Antibody Database (VAD) review about human tyrosinase, based on 29 published articles (read how Labome selects the articles), using tyrosinase antibody in all methods. It is aimed to help Labome visitors find the most suited tyrosinase antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
tyrosinase synonym: ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3

Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 8
  • western blot; mouse; 1:500; loading ...; fig 5a
Abcam tyrosinase antibody (Abcam, ab180753) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 8) and in western blot on mouse samples at 1:500 (fig 5a). Clin Cosmet Investig Dermatol (2021) ncbi
monoclonal
  • immunocytochemistry; human; loading ...; fig 3g
Abcam tyrosinase antibody (Abcam, ab738) was used in immunocytochemistry on human samples (fig 3g). Stem Cell Res Ther (2021) ncbi
domestic rabbit monoclonal (EPR10141)
  • western blot; human; loading ...; fig 4h
Abcam tyrosinase antibody (Abcam, ab170905) was used in western blot on human samples (fig 4h). Aging (Albany NY) (2019) ncbi
mouse monoclonal (HMB45 + M2-7C10 + M2-9E3 + T311)
  • immunohistochemistry - paraffin section; human; loading ...; fig 5a
Abcam tyrosinase antibody (Abcam, ab733) was used in immunohistochemistry - paraffin section on human samples (fig 5a). J Clin Invest (2018) ncbi
mouse monoclonal (PNL2)
  • immunohistochemistry - paraffin section; rat; 1:25; loading ...; fig st11
In order to outline the protocols for antibodies used for immunohistochemical studies, Abcam tyrosinase antibody (Abcam, ab12502) was used in immunohistochemistry - paraffin section on rat samples at 1:25 (fig st11). J Toxicol Pathol (2017) ncbi
mouse monoclonal (SPM360)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 8e
  • western blot; mouse; loading ...; fig 7a
In order to use stable isotope labeling with amino acids to measure the proteome in dihydrotestosterone- and 17beta-estradiol-treated human proximal tubular epithelial cells, Abcam tyrosinase antibody (Abcam, ab54447) was used in immunohistochemistry on mouse samples at 1:1000 (fig 8e) and in western blot on mouse samples (fig 7a). Mol Cell Proteomics (2017) ncbi
monoclonal
  • western blot; human; fig 4
In order to research pediatric large congenital melanocytic nevi to show melanocytes affect nodal expression and signaling in melanoma cells, Abcam tyrosinase antibody (Abcam, ab738) was used in western blot on human samples (fig 4). Int J Mol Sci (2016) ncbi
mouse monoclonal (HMB45 + M2-7C10 + M2-9E3 + T311)
  • immunohistochemistry - paraffin section; human; loading ...; fig 2
Abcam tyrosinase antibody (Abcam, ab733) was used in immunohistochemistry - paraffin section on human samples (fig 2). J Immunother Cancer (2015) ncbi
monoclonal
  • western blot; mouse; fig 4
In order to study engineered anti-melanoma vaccines that modulate cytokine priming and silence PD-L1 simultaneously by use of ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy, Abcam tyrosinase antibody (Abcam, ab738) was used in western blot on mouse samples (fig 4). Oncoimmunology (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (T311)
  • western blot; human; 1:200; loading ...
In order to study the function of the RAB6 secretory pathway melanosome formation and maturation, Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz Biotechnology, sc-20035) was used in western blot on human samples at 1:200. Nat Commun (2017) ncbi
mouse monoclonal (T311)
  • western blot; human; 1:1000; loading ...; fig 6b
In order to identify conditions in which low-passage ARPE-19 cells express genes specific to native human RPE cells, Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz, SC-20035) was used in western blot on human samples at 1:1000 (fig 6b). Mol Vis (2017) ncbi
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 2c
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz, T311) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2c). Nat Commun (2016) ncbi
mouse monoclonal (T311)
  • western blot; human; fig 1
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz, sc-20035) was used in western blot on human samples (fig 1). Biomed Rep (2016) ncbi
mouse monoclonal (T311)
  • western blot; human; 1:1000; fig 3c
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz, 20035) was used in western blot on human samples at 1:1000 (fig 3c). PLoS ONE (2015) ncbi
mouse monoclonal (0.N.596)
  • western blot; human; fig 3
  • western blot; dogs; fig 3
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz Biotechnology, sc-73243) was used in western blot on human samples (fig 3) and in western blot on dogs samples (fig 3). Vet Comp Oncol (2016) ncbi
mouse monoclonal (T311)
  • western blot; human
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz, SC-20035) was used in western blot on human samples . Mol Vis (2014) ncbi
mouse monoclonal (T311)
  • western blot; human
Santa Cruz Biotechnology tyrosinase antibody (Santa Cruz Biotechnology, sc-20035) was used in western blot on human samples . PLoS ONE (2012) ncbi
Invitrogen
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1b
  • western blot; human; 1:2000; loading ...; fig 8a
Invitrogen tyrosinase antibody (Thermo Fisher, MS-800-P1) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1b) and in western blot on human samples at 1:2000 (fig 8a). Nat Commun (2019) ncbi
mouse monoclonal (T311)
  • flow cytometry; human; fig 1d
In order to identify NFATc2 as a regulator of human melanoma dedifferentiation, Invitrogen tyrosinase antibody (Life Technologies, MA5-14177) was used in flow cytometry on human samples (fig 1d). Oncogene (2016) ncbi
mouse monoclonal (T311)
  • western blot; human
In order to identify antigens recognized by autoreactive CD4 T lymphocytes isolated from a Vogt-Koyanagi-Harada patient who did not express HLA-DRB1*04:05, Invitrogen tyrosinase antibody (Thermo Scientific, MS800P1) was used in western blot on human samples . Mol Vis (2014) ncbi
mouse monoclonal (T311)
  • immunocytochemistry; mouse; 1:50
  • western blot; mouse; 1:1000
Invitrogen tyrosinase antibody (Invitrogen, 35-600) was used in immunocytochemistry on mouse samples at 1:50 and in western blot on mouse samples at 1:1000. Arch Dermatol Res (2014) ncbi
mouse monoclonal (T311)
  • flow cytometry; human; 1:50; fig 2
In order to develop a quantitative assay to assess tumor-infiltrating lymphocytes purity, Invitrogen tyrosinase antibody (Invitrogen, 35-6000) was used in flow cytometry on human samples at 1:50 (fig 2). Cytometry A (2012) ncbi
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; tbl 4
In order to investigate T--cell effector function in relation to tumor-escape mechanisms, Invitrogen tyrosinase antibody (Zymed, clone T311) was used in immunohistochemistry - paraffin section on human samples (tbl 4). Clin Cancer Res (2011) ncbi
mouse monoclonal (T311)
  • western blot; human; 1:500; fig 2
In order to examine the subproteome of mitochondria isolated from human donor retinal pigment epithelium graded with the Minnesota Grading System, Invitrogen tyrosinase antibody (Zymed, 35-6000) was used in western blot on human samples at 1:500 (fig 2). Invest Ophthalmol Vis Sci (2008) ncbi
Cell Marque
mouse monoclonal (T311)
  • flow cytometry; human; loading ...; fig s3b
Cell Marque tyrosinase antibody (Cell Marque, T311) was used in flow cytometry on human samples (fig s3b). Cancer Immunol Immunother (2020) ncbi
Dako
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 4d
Dako tyrosinase antibody (Dako, T311) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4d). Nat Commun (2018) ncbi
Cell Signaling Technology
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; fig 1c
In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Cell Signaling Technology tyrosinase antibody (CST, 9319) was used in immunohistochemistry - paraffin section on human samples (fig 1c). Cell Rep (2016) ncbi
Leica Biosystems
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; loading ...; fig s1
Leica Biosystems tyrosinase antibody (Leica Biosystems, T311) was used in immunohistochemistry - paraffin section on human samples (fig s1). J Dermatol Sci (2017) ncbi
mouse monoclonal (T311)
  • immunohistochemistry - paraffin section; human; 1:20; loading ...; fig 2a
In order to identify immunogenic subset of metastatic uveal melanoma, Leica Biosystems tyrosinase antibody (Novocastra Division, NCL-TYROS) was used in immunohistochemistry - paraffin section on human samples at 1:20 (fig 2a). Clin Cancer Res (2016) ncbi
Articles Reviewed
  1. Zhai X, Gong M, Peng Y, Yang D. Effects of UV Induced-Photoaging on the Hair Follicle Cycle of C57BL6/J Mice. Clin Cosmet Investig Dermatol. 2021;14:527-539 pubmed publisher
  2. Surendran H, Nandakumar S, Reddy K V, Stoddard J, Mohan K V, Upadhyay P, et al. Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration. Stem Cell Res Ther. 2021;12:70 pubmed publisher
  3. Boudewijns S, Bloemendal M, de Haas N, Westdorp H, Bol K, Schreibelt G, et al. Autologous monocyte-derived DC vaccination combined with cisplatin in stage III and IV melanoma patients: a prospective, randomized phase 2 trial. Cancer Immunol Immunother. 2020;69:477-488 pubmed publisher
  4. Tang L, Li J, Fu W, Wu W, Xu J. Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo. Aging (Albany NY). 2019;11:11829-11843 pubmed publisher
  5. Carballo Carbajal I, Laguna A, Romero Gimenez J, Cuadros T, Bove J, Martinez Vicente M, et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat Commun. 2019;10:973 pubmed publisher
  6. Zhu B, Chen S, Wang H, Yin C, Han C, Peng C, et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun. 2018;9:259 pubmed publisher
  7. Lin H, Wei S, Hurt E, Green M, Zhao L, Vatan L, et al. Host expression of PD-L1 determines efficacy of PD-L1 pathway blockade-mediated tumor regression. J Clin Invest. 2018;128:805-815 pubmed publisher
  8. Patwardhan A, Bardin S, Miserey Lenkei S, Larue L, Goud B, Raposo G, et al. Routing of the RAB6 secretory pathway towards the lysosome related organelle of melanocytes. Nat Commun. 2017;8:15835 pubmed publisher
  9. Samuel W, Jaworski C, Postnikova O, Kutty R, Duncan T, Tan L, et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis. 2017;23:60-89 pubmed
  10. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  11. Clotet S, Soler M, Riera M, Pascual J, Fang F, Zhou J, et al. Stable Isotope Labeling with Amino Acids (SILAC)-Based Proteomics of Primary Human Kidney Cells Reveals a Novel Link between Male Sex Hormones and Impaired Energy Metabolism in Diabetic Kidney Disease. Mol Cell Proteomics. 2017;16:368-385 pubmed publisher
  12. Bassani Sternberg M, Bräunlein E, Klar R, Engleitner T, Sinitcyn P, Audehm S, et al. Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nat Commun. 2016;7:13404 pubmed publisher
  13. Kaji T, Yamasaki O, Takata M, Otsuka M, Hamada T, Morizane S, et al. Comparative study on driver mutations in primary and metastatic melanomas at a single Japanese institute: A clue for intra- and inter-tumor heterogeneity. J Dermatol Sci. 2017;85:51-57 pubmed publisher
  14. Wang J, Pei Y, Xu H, Li L, Wang Y, Liu G, et al. Effects of bavachin and its regulation of melanin synthesis in A375 cells. Biomed Rep. 2016;5:87-92 pubmed
  15. Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  16. Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
  17. Rothermel L, Sabesan A, Stephens D, Chandran S, Paria B, Srivastava A, et al. Identification of an Immunogenic Subset of Metastatic Uveal Melanoma. Clin Cancer Res. 2016;22:2237-49 pubmed publisher
  18. Feng Z, Puri S, Moudgil T, Wood W, Hoyt C, Wang C, et al. Multispectral imaging of formalin-fixed tissue predicts ability to generate tumor-infiltrating lymphocytes from melanoma. J Immunother Cancer. 2015;3:47 pubmed publisher
  19. Perotti V, Baldassari P, Molla A, Vegetti C, Bersani I, Maurichi A, et al. NFATc2 is an intrinsic regulator of melanoma dedifferentiation. Oncogene. 2016;35:2862-72 pubmed publisher
  20. Liechtenstein T, Perez Janices N, Blanco Luquin I, Goyvaerts C, Schwarze J, Dufait I, et al. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy. Oncoimmunology. 2014;3:e945378 pubmed
  21. Arts N, Cané S, Hennequart M, Lamy J, Bommer G, Van den Eynde B, et al. microRNA-155, induced by interleukin-1ß, represses the expression of microphthalmia-associated transcription factor (MITF-M) in melanoma cells. PLoS ONE. 2015;10:e0122517 pubmed publisher
  22. Noguchi S, Kumazaki M, Mori T, Baba K, Okuda M, Mizuno T, et al. Analysis of microRNA-203 function in CREB/MITF/RAB27a pathway: comparison between canine and human melanoma cells. Vet Comp Oncol. 2016;14:384-394 pubmed publisher
  23. Abad S, Wieërs G, Colau D, Wildmann C, Delair E, Dhote R, et al. Absence of recognition of common melanocytic antigens by T cells isolated from the cerebrospinal fluid of a Vogt-Koyanagi-Harada patient. Mol Vis. 2014;20:956-69 pubmed
  24. Poliakov E, Strunnikova N, Jiang J, Martinez B, Parikh T, Lakkaraju A, et al. Multiple A2E treatments lead to melanization of rod outer segment-challenged ARPE-19 cells. Mol Vis. 2014;20:285-300 pubmed
  25. Nakajima H, Nagata T, Koga S, Imokawa G. Reduced glutathione disrupts the intracellular trafficking of tyrosinase and tyrosinase-related protein-1 but not dopachrome tautomerase and Pmel17 to melanosomes, which results in the attenuation of melanization. Arch Dermatol Res. 2014;306:37-49 pubmed publisher
  26. Marin M, Ghenea S, Spiridon L, Chiritoiu G, Petrescu A, Petrescu S. Tyrosinase degradation is prevented when EDEM1 lacks the intrinsically disordered region. PLoS ONE. 2012;7:e42998 pubmed publisher
  27. Richards J, Treisman J, Garlie N, Hanson J, Oaks M. Flow cytometry assessment of residual melanoma cells in tumor-infiltrating lymphocyte cultures. Cytometry A. 2012;81:374-81 pubmed publisher
  28. Tjin E, Konijnenberg D, Krebbers G, Mallo H, Drijfhout J, Franken K, et al. T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy. Clin Cancer Res. 2011;17:5736-47 pubmed publisher
  29. Nordgaard C, Karunadharma P, Feng X, Olsen T, Ferrington D. Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration. Invest Ophthalmol Vis Sci. 2008;49:2848-55 pubmed publisher