This is a Validated Antibody Database (VAD) review about human tryptase, based on 25 published articles (read how Labome selects the articles), using tryptase antibody in all methods. It is aimed to help Labome visitors find the most suited tryptase antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
tryptase synonym: TPS1; TPS2; TPSB1; TPSB2; Tryptase-2

Invitrogen
mouse monoclonal (AA1)
  • ELISA; human; tbl 1
In order to review how mast cells contribute to multiple myeloma, Invitrogen tryptase antibody (Thermo Scientific, AA1) was used in ELISA on human samples (tbl 1). Int Arch Allergy Immunol (2015) ncbi
mouse monoclonal (AA1)
  • immunocytochemistry; human
In order to develop a human mast cell culture system and study the effect of Macrophage Migration Inhibitory Factor supplementation, Invitrogen tryptase antibody (Thermo Scientific Pierce Antibodies, MA5-11711) was used in immunocytochemistry on human samples . PLoS ONE (2015) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry; human
In order to develop methods to study the role of mast cells in cancer, Invitrogen tryptase antibody (Thermo Scientifi, MS-1216) was used in immunohistochemistry on human samples . Methods Mol Biol (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-9)
  • immunohistochemistry - frozen section; human; 1:1000; fig 4
Santa Cruz Biotechnology tryptase antibody (Santa Cruz, Sc271095) was used in immunohistochemistry - frozen section on human samples at 1:1000 (fig 4). Neurogastroenterol Motil (2016) ncbi
mouse monoclonal (G3)
  • immunohistochemistry; human
Santa Cruz Biotechnology tryptase antibody (Santa Cruz Biotech, sc-33676) was used in immunohistochemistry on human samples . Mol Oncol (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR9522)
  • immunohistochemistry; mouse; 1:300; loading ...; fig 1
Abcam tryptase antibody (Abcam, ab151757) was used in immunohistochemistry on mouse samples at 1:300 (fig 1). PLoS ONE (2017) ncbi
MilliporeSigma
mouse monoclonal (G3)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3c
MilliporeSigma tryptase antibody (Sigma, 342M-17) was used in immunohistochemistry - paraffin section on human samples (fig 3c). Sci Adv (2019) ncbi
Dako
mouse monoclonal (AA1)
  • immunohistochemistry; human; loading ...; fig 1c
Dako tryptase antibody (Dako, 7052) was used in immunohistochemistry on human samples (fig 1c). Oncogene (2017) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; fig s1
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - paraffin section on human samples (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; tbl 1
In order to investigate IL-17 production in oral squamous cell carcinoma, Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). J Oral Pathol Med (2017) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; tbl 1
In order to measure tryptase, MMP-9, and MMP-13 expression in periapical lesions and correlate expression with the type of lesion, intensity of the inflammatory infiltrate, and thickness of the epithelial lining, Dako tryptase antibody (Dako, AA1) was used in immunohistochemistry - paraffin section on human samples at 1:200 (tbl 1). Int Endod J (2017) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - frozen section; human; fig 4
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - frozen section on human samples (fig 4). Br J Dermatol (2016) ncbi
mouse monoclonal (AA1)
  • western blot; human
Dako tryptase antibody (Dako, M7052) was used in western blot on human samples . Br J Dermatol (2015) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; 1:3000
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - paraffin section on human samples at 1:3000. Clin Gastroenterol Hepatol (2014) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry; zebrafish
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry on zebrafish samples . Br J Haematol (2014) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry; human
Dako tryptase antibody (DakoCytomation, AA1) was used in immunohistochemistry on human samples . J Allergy Clin Immunol (2014) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry; human; 1:200; loading ...; fig 6a
In order to discuss features of interstitial cell populations in the lamina propria of the bladder, Dako tryptase antibody (Dako, AA1) was used in immunohistochemistry on human samples at 1:200 (fig 6a). J Urol (2014) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; tbl 1
In order to study the effect of innate lymphoid cells on B cells, Dako tryptase antibody (Dako, AA1) was used in immunohistochemistry - paraffin section on human samples (tbl 1). Nat Immunol (2014) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; human; 1:1000
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - paraffin section on human samples at 1:1000. Am J Respir Crit Care Med (2012) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - paraffin section; cat
  • immunocytochemistry; cat
Dako tryptase antibody (Dako, AA1) was used in immunohistochemistry - paraffin section on cat samples and in immunocytochemistry on cat samples . Can Vet J (2011) ncbi
mouse monoclonal (AA1)
  • immunohistochemistry - frozen section; rat; 1:100
Dako tryptase antibody (Dako, M7052) was used in immunohistochemistry - frozen section on rat samples at 1:100. J Comp Neurol (2008) ncbi
EMD Millipore
mouse monoclonal (G3)
  • flow cytometry; human; fig 3
EMD Millipore tryptase antibody (Chemicon, MAB1222) was used in flow cytometry on human samples (fig 3). PLoS ONE (2016) ncbi
mouse monoclonal (G3)
  • immunohistochemistry - frozen section; human; 1:1000; loading ...; tbl 1
In order to examine immune cells and chemokines present in human second-trimester fetal skin, EMD Millipore tryptase antibody (Millipore, G3) was used in immunohistochemistry - frozen section on human samples at 1:1000 (tbl 1). Wound Repair Regen (2016) ncbi
mouse monoclonal (G3)
  • immunocytochemistry; human
EMD Millipore tryptase antibody (Chemicon International, G3) was used in immunocytochemistry on human samples . FASEB J (2014) ncbi
mouse monoclonal (G3)
  • immunohistochemistry - paraffin section; human; 1:12000
EMD Millipore tryptase antibody (Chemicon, G3) was used in immunohistochemistry - paraffin section on human samples at 1:12000. Clin Exp Allergy (2014) ncbi
Leica Biosystems
  • immunohistochemistry - paraffin section; human; 1:300; fig 1e
In order to measure neuropeptide Y, NPY receptor 1, and NPY receptor 2 expression in infantile haemangiomas, Leica Biosystems tryptase antibody (Leica, NCL-MCTRYP-428) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 1e). Acta Paediatr (2017) ncbi
Articles Reviewed
  1. Izadi D, Layton T, Williams L, McCann F, Cabrita M, Espirito Santo A, et al. Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis. Sci Adv. 2019;5:eaay0370 pubmed publisher
  2. Ramirez GarciaLuna J, Chan D, Samberg R, Abou Rjeili M, Wong T, Li A, et al. Defective bone repair in mast cell-deficient Cpa3Cre/+ mice. PLoS ONE. 2017;12:e0174396 pubmed publisher
  3. Chen Y, Li C, Xie H, Fan Y, Yang Z, Ma J, et al. Infiltrating mast cells promote renal cell carcinoma angiogenesis by modulating PI3K→︀AKT→︀GSK3β→︀AM signaling. Oncogene. 2017;36:2879-2888 pubmed publisher
  4. Tan E, Blackwell M, Dunne J, Marsh R, Tan S, Itinteang T. Neuropeptide Y receptor 1 is expressed by B and T lymphocytes and mast cells in infantile haemangiomas. Acta Paediatr. 2017;106:292-297 pubmed publisher
  5. Kirshenbaum A, Cruse G, Desai A, Bandara G, Leerkes M, Lee C, et al. Immunophenotypic and Ultrastructural Analysis of Mast Cells in Hermansky-Pudlak Syndrome Type-1: A Possible Connection to Pulmonary Fibrosis. PLoS ONE. 2016;11:e0159177 pubmed publisher
  6. Avadhani A, Parachuru V, Milne T, Seymour G, Rich A. Multiple cells express interleukin 17 in oral squamous cell carcinoma. J Oral Pathol Med. 2017;46:39-45 pubmed publisher
  7. Yu Y, Daly D, Adam I, Kitsanta P, Hill C, Wild J, et al. Interplay between mast cells, enterochromaffin cells, and sensory signaling in the aging human bowel. Neurogastroenterol Motil. 2016;28:1465-79 pubmed publisher
  8. Andrade A, Santos E, Carmo A, Freitas R, Galvão H. Analysis of tryptase-positive mast cells and immunoexpression of MMP-9 and MMP-13 in periapical lesions. Int Endod J. 2017;50:446-454 pubmed publisher
  9. Vyzoukaki R, Tsirakis G, Pappa C, Devetzoglou M, Tzardi M, Alexandrakis M. The Impact of Mast Cell Density on the Progression of Bone Disease in Multiple Myeloma Patients. Int Arch Allergy Immunol. 2015;168:263-8 pubmed publisher
  10. Walraven M, Talhout W, Beelen R, van Egmond M, Ulrich M. Healthy human second-trimester fetal skin is deficient in leukocytes and associated homing chemokines. Wound Repair Regen. 2016;24:533-41 pubmed publisher
  11. Yan K, Huang Q, Fang X, Zhang Z, Han L, Gadaldi K, et al. IgE and FcεRI are highly expressed on innate cells in psoriasis. Br J Dermatol. 2016;175:122-33 pubmed publisher
  12. Wang F, Calderone K, Smith N, Do T, Helfrich Y, Johnson T, et al. Marked disruption and aberrant regulation of elastic fibres in early striae gravidarum. Br J Dermatol. 2015;173:1420-30 pubmed publisher
  13. Ningyan G, Xu Y, Hongfei S, Jingjing C, Min C. The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production. PLoS ONE. 2015;10:e0122482 pubmed publisher
  14. Blatner N, Tsai F, Khazaie K. Methods for the study of mast cells in cancer. Methods Mol Biol. 2015;1220:443-60 pubmed publisher
  15. Dellon E, Speck O, Woodward K, Covey S, Rusin S, Gebhart J, et al. Markers of eosinophilic inflammation for diagnosis of eosinophilic esophagitis and proton pump inhibitor-responsive esophageal eosinophilia: a prospective study. Clin Gastroenterol Hepatol. 2014;12:2015-22 pubmed publisher
  16. Balci T, Prykhozhij S, Teh E, Da as S, McBride E, Liwski R, et al. A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis. Br J Haematol. 2014;167:48-61 pubmed publisher
  17. Fujisawa D, Kashiwakura J, Kita H, Kikukawa Y, Fujitani Y, Sasaki Sakamoto T, et al. Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. J Allergy Clin Immunol. 2014;134:622-633.e9 pubmed publisher
  18. Gevaert T, Vanstreels E, Daelemans D, Franken J, Van Der Aa F, Roskams T, et al. Identification of different phenotypes of interstitial cells in the upper and deep lamina propria of the human bladder dome. J Urol. 2014;192:1555-63 pubmed publisher
  19. Hoermann G, Blatt K, Greiner G, Putz E, Berger A, Herrmann H, et al. CD52 is a molecular target in advanced systemic mastocytosis. FASEB J. 2014;28:3540-51 pubmed publisher
  20. Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-364 pubmed publisher
  21. Shikhagaie M, Andersson C, Mori M, Kortekaas Krohn I, Bergqvist A, Dahl R, et al. Mapping of TLR5 and TLR7 in central and distal human airways and identification of reduced TLR expression in severe asthma. Clin Exp Allergy. 2014;44:184-96 pubmed publisher
  22. Polajeva J, Bergström T, Edqvist P, Lundequist A, Sjösten A, Nilsson G, et al. Glioma-derived macrophage migration inhibitory factor (MIF) promotes mast cell recruitment in a STAT5-dependent manner. Mol Oncol. 2014;8:50-8 pubmed publisher
  23. Bhattacharya S, Go D, Krenitsky D, Huyck H, Solleti S, Lunger V, et al. Genome-wide transcriptional profiling reveals connective tissue mast cell accumulation in bronchopulmonary dysplasia. Am J Respir Crit Care Med. 2012;186:349-58 pubmed publisher
  24. Gilroy C, Forzán M, Drew A, Vernau W. Eosinophilia in a cat with acute leukemia. Can Vet J. 2011;52:1004-8 pubmed
  25. Lennerz J, Rühle V, Ceppa E, Neuhuber W, Bunnett N, Grady E, et al. Calcitonin receptor-like receptor (CLR), receptor activity-modifying protein 1 (RAMP1), and calcitonin gene-related peptide (CGRP) immunoreactivity in the rat trigeminovascular system: differences between peripheral and central CGRP receptor distribu. J Comp Neurol. 2008;507:1277-99 pubmed publisher