This is a Validated Antibody Database (VAD) review about human topoisomerase I, based on 18 published articles (read how Labome selects the articles), using topoisomerase I antibody in all methods. It is aimed to help Labome visitors find the most suited topoisomerase I antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
topoisomerase I synonym: TOPI

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5375)
  • western blot knockout validation; human; loading ...; fig s5f
  • immunocytochemistry; mouse; fig 4d
Abcam topoisomerase I antibody (Abcam, ab109374) was used in western blot knockout validation on human samples (fig s5f) and in immunocytochemistry on mouse samples (fig 4d). Mol Cancer (2021) ncbi
BD Biosciences
mouse monoclonal (C-21)
  • immunohistochemistry; human; fig 3
  • western blot; human; fig 3
  • immunohistochemistry knockout validation; mouse; fig 3
  • western blot knockout validation; mouse; fig 3
In order to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion, BD Biosciences topoisomerase I antibody (BD Pharmingen, 556597) was used in immunohistochemistry on human samples (fig 3), in western blot on human samples (fig 3), in immunohistochemistry knockout validation on mouse samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Nucleic Acids Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-21)
  • western blot; human; loading ...; fig 4c
Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, sc-32736) was used in western blot on human samples (fig 4c). Sci Rep (2020) ncbi
mouse monoclonal (C-21)
  • western blot; human; fig 1a
Santa Cruz Biotechnology topoisomerase I antibody (SantaCruz, sc-32736) was used in western blot on human samples (fig 1a). Cell Death Dis (2018) ncbi
mouse monoclonal (C-21)
  • western blot; human; loading ...; fig s2a
Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, SC-32736) was used in western blot on human samples (fig s2a). Nucleic Acids Res (2017) ncbi
mouse monoclonal (C-21)
  • western blot; human; fig 1
In order to study how PARylation regulates Top1 nuclear dynamics, Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, C21) was used in western blot on human samples (fig 1). Nucleic Acids Res (2016) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 4a
In order to measure Dicer protein levels in various types of breast cancer samples, Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, C-21) was used in western blot on human samples (fig 4a). Hum Pathol (2016) ncbi
mouse monoclonal (C-21)
  • western blot; human; loading ...; fig 4a
In order to measure Dicer protein levels in various types of breast cancer samples, Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, C-21) was used in western blot on human samples (fig 4a). Hum Pathol (2016) ncbi
mouse monoclonal (H-5)
  • western blot; mouse; 1:250; fig s1
Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, sc-271285) was used in western blot on mouse samples at 1:250 (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (C-21)
  • flow cytometry; human; fig 4
  • western blot; human; fig 4
Santa Cruz Biotechnology topoisomerase I antibody (Santa Cruz, sc-32736) was used in flow cytometry on human samples (fig 4) and in western blot on human samples (fig 4). Mol Med Rep (2015) ncbi
Abcam
domestic rabbit monoclonal (EPR5375)
  • western blot knockout validation; human; loading ...; fig s5f
  • immunocytochemistry; mouse; fig 4d
Abcam topoisomerase I antibody (Abcam, ab109374) was used in western blot knockout validation on human samples (fig s5f) and in immunocytochemistry on mouse samples (fig 4d). Mol Cancer (2021) ncbi
domestic rabbit monoclonal (EPR5375)
  • western blot; human; 1:5000; loading ...; fig s6c
Abcam topoisomerase I antibody (Abcam, ab109374) was used in western blot on human samples at 1:5000 (fig s6c). Nature (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Abcam topoisomerase I antibody (Abcam, ab3825) was used in western blot on human samples (fig 1b). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:400; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Abcam topoisomerase I antibody (Abcam, ab85038) was used in western blot on human samples at 1:400 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR5375)
  • immunoprecipitation; human; fig 4
Abcam topoisomerase I antibody (Abcam, ab109374) was used in immunoprecipitation on human samples (fig 4). Cell Rep (2016) ncbi
domestic rabbit monoclonal (EPR5375)
  • western blot; human; fig 6
Abcam topoisomerase I antibody (Abcam, ab109374) was used in western blot on human samples (fig 6). Nucleic Acids Res (2016) ncbi
BD Biosciences
mouse monoclonal (C-21)
  • western blot; human; fig 4
BD Biosciences topoisomerase I antibody (BD Pharmingen, 556597) was used in western blot on human samples (fig 4). Cell Rep (2016) ncbi
mouse monoclonal (C-21)
  • western blot; human; fig 2
BD Biosciences topoisomerase I antibody (BD Pharmingen, C-21) was used in western blot on human samples (fig 2). Biochem J (2016) ncbi
mouse monoclonal (C-21)
  • western blot; human; 1:1000; loading ...; fig 2
In order to present the role of bromodomain and hedgehog pathway on small cell lung cancer, BD Biosciences topoisomerase I antibody (BD Pharmingen, 556597) was used in western blot on human samples at 1:1000 (fig 2). Cancer Lett (2016) ncbi
mouse monoclonal (C-21)
  • western blot; mouse
BD Biosciences topoisomerase I antibody (BD Biosciences, 556597) was used in western blot on mouse samples . Nat Struct Mol Biol (2015) ncbi
mouse monoclonal (C-21)
  • western blot; human; 1:2000
In order to evaluate the therapeutic potential of oligonucleotide F10 against against acute lymphoblastic leukemia, BD Biosciences topoisomerase I antibody (BD Pharmingen, 556597) was used in western blot on human samples at 1:2000. Oncotarget (2014) ncbi
mouse monoclonal (C-21)
  • immunohistochemistry; human; fig 3
  • western blot; human; fig 3
  • immunohistochemistry knockout validation; mouse; fig 3
  • western blot knockout validation; mouse; fig 3
In order to study the roles of Top2-alpha and -beta in mitochondria and the effect on mitochondrial DNA supercoiling of Top1mt deletion, BD Biosciences topoisomerase I antibody (BD Pharmingen, 556597) was used in immunohistochemistry on human samples (fig 3), in western blot on human samples (fig 3), in immunohistochemistry knockout validation on mouse samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Nucleic Acids Res (2014) ncbi
Articles Reviewed
  1. Gu Y, Wang Y, He L, Zhang J, Zhu X, Liu N, et al. Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Mol Cancer. 2021;20:132 pubmed publisher
  2. Oo J, Irmer B, Günther S, Warwick T, Palfi K, Izquierdo Ponce J, et al. ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting. Sci Rep. 2020;10:19079 pubmed publisher
  3. Zimmermann M, Murina O, Reijns M, Agathanggelou A, Challis R, Tarnauskaitė Ž, et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature. 2018;559:285-289 pubmed publisher
  4. Makhov P, Naito S, Haifler M, Kutikov A, Boumber Y, Uzzo R, et al. The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma. Cell Death Dis. 2018;9:374 pubmed publisher
  5. Meisenberg C, Ashour M, El Shafie L, Liao C, Hodgson A, Pilborough A, et al. Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Nucleic Acids Res. 2017;45:1159-1176 pubmed publisher
  6. Ahmad M, Shen W, Li W, Xue Y, Zou S, Xu D, et al. Topoisomerase 3? is the major topoisomerase for mRNAs and linked to neurodevelopment and mental dysfunction. Nucleic Acids Res. 2017;45:2704-2713 pubmed publisher
  7. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  8. Das S, Rehman I, Ghosh A, Sengupta S, Majumdar P, Jana B, et al. Poly(ADP-ribose) polymers regulate DNA topoisomerase I (Top1) nuclear dynamics and camptothecin sensitivity in living cells. Nucleic Acids Res. 2016;44:8363-75 pubmed publisher
  9. Spoelstra N, Cittelly D, Christenson J, Gordon M, Elias A, Jedlicka P, et al. Dicer expression in estrogen receptor-positive versus triple-negative breast cancer: an antibody comparison. Hum Pathol. 2016;56:40-51 pubmed publisher
  10. Mabb A, Simon J, KING I, Lee H, An L, Philpot B, et al. Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PLoS ONE. 2016;11:e0156439 pubmed publisher
  11. Ribeyre C, Zellweger R, Chauvin M, Bec N, Larroque C, Lopes M, et al. Nascent DNA Proteomics Reveals a Chromatin Remodeler Required for Topoisomerase I Loading at Replication Forks. Cell Rep. 2016;15:300-9 pubmed publisher
  12. Vincent A, Berthel E, Dacheux E, Magnard C, Venezia N. BRCA1 affects protein phosphatase 6 signalling through its interaction with ANKRD28. Biochem J. 2016;473:949-60 pubmed publisher
  13. Kaur G, Reinhart R, Monks A, Evans D, Morris J, Polley E, et al. Bromodomain and hedgehog pathway targets in small cell lung cancer. Cancer Lett. 2016;371:225-39 pubmed publisher
  14. Cristini A, Park J, Capranico G, Legube G, Favre G, Sordet O. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2016;44:1161-78 pubmed publisher
  15. Hartlerode A, Morgan M, Wu Y, Buis J, Ferguson D. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat Struct Mol Biol. 2015;22:736-43 pubmed publisher
  16. Liu Y, Ban L, Su X, Gao S, Liu J, Cui X. Effects of cinobufacini injection on cell proliferation and the expression of topoisomerases in human HepG-2 hepatocellular carcinoma cells. Mol Med Rep. 2015;12:1598-604 pubmed publisher
  17. Pardee T, Stadelman K, Jennings Gee J, Caudell D, Gmeiner W. The poison oligonucleotide F10 is highly effective against acute lymphoblastic leukemia while sparing normal hematopoietic cells. Oncotarget. 2014;5:4170-9 pubmed
  18. Zhang H, Zhang Y, Yasukawa T, Dalla Rosa I, Khiati S, Pommier Y. Increased negative supercoiling of mtDNA in TOP1mt knockout mice and presence of topoisomerases II? and II? in vertebrate mitochondria. Nucleic Acids Res. 2014;42:7259-67 pubmed publisher