This is a Validated Antibody Database (VAD) review about human tie-2, based on 30 published articles (read how Labome selects the articles), using tie-2 antibody in all methods. It is aimed to help Labome visitors find the most suited tie-2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
tie-2 synonym: CD202B; GLC3E; TIE-2; TIE2; VMCM; VMCM1; angiopoietin-1 receptor; TEK tyrosine kinase, endothelial; endothelial tyrosine kinase; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2

Knockout validation
EMD Millipore
mouse monoclonal (Ab33)
  • western blot; mouse; fig 1b
  • western blot knockout validation; human; fig 1b
In order to use a co-delivery system of adenovirus encoding EGFP-tagged Cas9 and lentivirus encoding a single guide RNA in primary human lung microvascular endothelial cells to disrupt Tie2 gene and protein expression, EMD Millipore tie-2 antibody (Millipore, 05-584) was used in western blot on mouse samples (fig 1b) and in western blot knockout validation on human samples (fig 1b). Sci Rep (2017) ncbi
R&D Systems
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
R&D Systems tie-2 antibody (R&D Systems, AF2720) was used in immunohistochemistry - frozen section on mouse samples (fig 1a). J Clin Invest (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2c
  • western blot; human; loading ...; fig 2d
R&D Systems tie-2 antibody (R&D Systems, AF2720) was used in immunocytochemistry on human samples (fig 2c) and in western blot on human samples (fig 2d). Proc Natl Acad Sci U S A (2017) ncbi
goat polyclonal
  • immunoprecipitation; human; loading ...; fig 2d
  • western blot; human; loading ...; fig 2d
R&D Systems tie-2 antibody (R&D Systems, AF313) was used in immunoprecipitation on human samples (fig 2d) and in western blot on human samples (fig 2d). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5f
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 2h
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, R&D Systems tie-2 antibody (R&D, AF2720) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5f) and in immunohistochemistry - frozen section on human samples at 1:100 (fig 2h). Nat Med (2016) ncbi
mouse monoclonal (83715)
  • flow cytometry; human; loading ...; fig 2b
In order to determine the frequency and function of intermediate monocytes and TIE2-expressing monocytes during chemotherapy and anti-angiogenic cancer treatment, R&D Systems tie-2 antibody (R&D Systems, FAB3131P) was used in flow cytometry on human samples (fig 2b). Oncoimmunology (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:50; fig 9
  • western blot; mouse; 1:400; fig 8
In order to determine the rescue of VE-PTP-dependent restoration of Tie2 signaling from angiopoietin-2-induced blood-brain barrier compromise and increased stroke size, R&D Systems tie-2 antibody (R&D Systems, AF2720) was used in immunohistochemistry - free floating section on mouse samples at 1:50 (fig 9) and in western blot on mouse samples at 1:400 (fig 8). Acta Neuropathol (2016) ncbi
mouse monoclonal (83715)
  • flow cytometry; human; loading ...
R&D Systems tie-2 antibody (R&D Systems, FAB3131P) was used in flow cytometry on human samples . Clin Transl Immunology (2016) ncbi
mouse monoclonal (83715)
  • flow cytometry; human; loading ...; fig s1a
In order to study the role of distinct RNA transcriptome patterns in angiogenesis in Tie2-expressing monocytes, R&D Systems tie-2 antibody (R&D Systems, FAB3131A) was used in flow cytometry on human samples (fig s1a). Gene (2016) ncbi
mouse monoclonal (83715)
  • flow cytometry; human; fig 1
R&D Systems tie-2 antibody (R&D systems, FAB3131A) was used in flow cytometry on human samples (fig 1). PLoS ONE (2015) ncbi
goat polyclonal
  • immunocytochemistry; human; 1:100; fig s1
  • western blot; human; 1:4000; fig s1
R&D Systems tie-2 antibody (R&D systems, AF313) was used in immunocytochemistry on human samples at 1:100 (fig s1) and in western blot on human samples at 1:4000 (fig s1). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
R&D Systems tie-2 antibody (R&D Systems, AF2720) was used in western blot on rat samples at 1:1000. PLoS ONE (2014) ncbi
BioLegend
mouse monoclonal (33.1 (Ab33))
  • mass cytometry; human; fig s8
BioLegend tie-2 antibody (BioLegend, 334202) was used in mass cytometry on human samples (fig s8). Nature (2017) ncbi
mouse monoclonal (33.1 (Ab33))
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BioLegend tie-2 antibody (BioLegen d, 334206) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (33.1 (Ab33))
  • flow cytometry; mouse; fig 4
BioLegend tie-2 antibody (BioLegend, 334208) was used in flow cytometry on mouse samples (fig 4). Dev Biol (2015) ncbi
mouse monoclonal (33.1 (Ab33))
BioLegend tie-2 antibody (BioLegend, Ab33) was used . Nephrol Dial Transplant (2015) ncbi
Abcam
mouse monoclonal (Cl. 16)
  • western blot; human; loading ...; fig 4a
Abcam tie-2 antibody (Abcam, ab24859) was used in western blot on human samples (fig 4a). Lab Invest (2017) ncbi
mouse monoclonal (Cl. 16)
  • flow cytometry; mouse; fig s2b
  • immunocytochemistry; mouse; 1:500; fig st1
In order to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy, Abcam tie-2 antibody (Abcam, 24859) was used in flow cytometry on mouse samples (fig s2b) and in immunocytochemistry on mouse samples at 1:500 (fig st1). Circ Res (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig s1c
In order to study the role of distinct RNA transcriptome patterns in angiogenesis in Tie2-expressing monocytes, Abcam tie-2 antibody (Abcam, ab137786) was used in western blot on human samples (fig s1c). Gene (2016) ncbi
mouse monoclonal (Cl. 16)
  • western blot; human
Abcam tie-2 antibody (Abcam, ab24859) was used in western blot on human samples . Mol Syndromol (2013) ncbi
Novus Biologicals
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 7
Novus Biologicals tie-2 antibody (Novus Biologicals, NBP 1-69753) was used in immunohistochemistry on mouse samples at 1:200 (fig 7). Am J Transl Res (2016) ncbi
Cell Signaling Technology
mouse monoclonal (Ab33)
  • western blot; human; loading ...; fig s7b
Cell Signaling Technology tie-2 antibody (Cell Signaling, AB33) was used in western blot on human samples (fig s7b). Proc Natl Acad Sci U S A (2018) ncbi
mouse monoclonal (Ab33)
  • immunohistochemistry - paraffin section; human; fig 1I
Cell Signaling Technology tie-2 antibody (Cell Signaling, 4224) was used in immunohistochemistry - paraffin section on human samples (fig 1I). J Hematol Oncol (2017) ncbi
mouse monoclonal (Ab33)
  • immunohistochemistry - frozen section; human; 1:1000; loading ...; tbl 1
  • western blot; human; 1:1000; fig s4e
  • western blot; mouse; 1:1000; loading ...; fig 5i
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, Cell Signaling Technology tie-2 antibody (Cell Signaling, 4224) was used in immunohistochemistry - frozen section on human samples at 1:1000 (tbl 1), in western blot on human samples at 1:1000 (fig s4e) and in western blot on mouse samples at 1:1000 (fig 5i). Nat Med (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig s4h
  • western blot; mouse; 1:1000; loading ...; fig 5i
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, Cell Signaling Technology tie-2 antibody (Cell Signaling, 4221) was used in western blot on human samples at 1:1000 (fig s4h) and in western blot on mouse samples at 1:1000 (fig 5i). Nat Med (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5f
  • immunohistochemistry - frozen section; human; 1:1000; loading ...; fig 2h
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, Cell Signaling Technology tie-2 antibody (Cell Signaling, 4226) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 5f) and in immunohistochemistry - frozen section on human samples at 1:1000 (fig 2h). Nat Med (2016) ncbi
mouse monoclonal (Ab33)
  • western blot; human; fig s3e
Cell Signaling Technology tie-2 antibody (Cell Signaling, 4224) was used in western blot on human samples (fig s3e). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology tie-2 antibody (Cell Signaling, 4221) was used in western blot on human samples (fig 4). Endocr Relat Cancer (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3a
Cell Signaling Technology tie-2 antibody (Cell Signaling, 4221) was used in western blot on human samples (fig 3a). PLoS ONE (2016) ncbi
rabbit monoclonal (D9D10)
  • western blot; human; fig 3a
Cell Signaling Technology tie-2 antibody (Cell Signaling, 7403) was used in western blot on human samples (fig 3a). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology tie-2 antibody (cell signaling, 4226s) was used in western blot on human samples (fig 5a). Cancer Res (2016) ncbi
mouse monoclonal (Ab33)
  • western blot; human; fig 7
Cell Signaling Technology tie-2 antibody (Cell Signaling Technology, #4224) was used in western blot on human samples (fig 7). Am J Physiol Heart Circ Physiol (2015) ncbi
EMD Millipore
mouse monoclonal (Ab33)
  • western blot; mouse; fig 1b
  • western blot knockout validation; human; fig 1b
In order to use a co-delivery system of adenovirus encoding EGFP-tagged Cas9 and lentivirus encoding a single guide RNA in primary human lung microvascular endothelial cells to disrupt Tie2 gene and protein expression, EMD Millipore tie-2 antibody (Millipore, 05-584) was used in western blot on mouse samples (fig 1b) and in western blot knockout validation on human samples (fig 1b). Sci Rep (2017) ncbi
mouse monoclonal (Ab33)
  • western blot; rat; 1:1000; loading ...; fig 6a
In order to elucidate how miR-21-5p alleviates traumatic brain injury in rats, EMD Millipore tie-2 antibody (Millipore, 05-584) was used in western blot on rat samples at 1:1000 (fig 6a). Brain Res (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
EMD Millipore tie-2 antibody (Millipore, ABS219) was used in western blot on mouse samples at 1:1000 (fig 3). Matrix Biol (2015) ncbi
mouse monoclonal (Ab33)
  • western blot; mouse
EMD Millipore tie-2 antibody (Upstate Biotechnology, 05-584) was used in western blot on mouse samples . Mol Ther (2014) ncbi
BD Biosciences
mouse monoclonal (33)
  • immunohistochemistry; mouse
BD Biosciences tie-2 antibody (BD Pharmingen, 557039) was used in immunohistochemistry on mouse samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. Souma T, Thomson B, Heinen S, Carota I, Yamaguchi S, Onay T, et al. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proc Natl Acad Sci U S A. 2018;115:1298-1303 pubmed publisher
  2. Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  3. Leppänen V, Saharinen P, Alitalo K. Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization. Proc Natl Acad Sci U S A. 2017;114:4376-4381 pubmed publisher
  4. Wang K, Liu W, Song Y, Wu X, Zhang Y, Li S, et al. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration. Lab Invest. 2017;97:971-982 pubmed publisher
  5. Lu X, Horner J, Paul E, Shang X, Troncoso P, Deng P, et al. Effective combinatorial immunotherapy for castration-resistant prostate cancer. Nature. 2017;543:728-732 pubmed publisher
  6. Gong H, Liu M, Klomp J, Merrill B, Rehman J, Malik A. Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells. Sci Rep. 2017;7:42127 pubmed publisher
  7. Gravina G, Mancini A, Marampon F, Colapietro A, Delle Monache S, Sferra R, et al. The brain-penetrating CXCR4 antagonist, PRX177561, increases the antitumor effects of bevacizumab and sunitinib in preclinical models of human glioblastoma. J Hematol Oncol. 2017;10:5 pubmed publisher
  8. Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher
  9. Schauer D, Starlinger P, Alidzanovic L, Zajc P, Maier T, Feldman A, et al. Chemotherapy of colorectal liver metastases induces a rapid rise in intermediate blood monocytes which predicts treatment response. Oncoimmunology. 2016;5:e1160185 pubmed publisher
  10. Ge X, Huang S, Gao H, Han Z, Chen F, Zhang S, et al. miR-21-5p alleviates leakage of injured brain microvascular endothelial barrier in vitro through suppressing inflammation and apoptosis. Brain Res. 2016;1650:31-40 pubmed publisher
  11. Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
  12. Han H, Kim B, Lee J, Kang S, Kim J, Cho N. Angiopoietin-2 promotes ER+ breast cancer cell survival in bone marrow niche. Endocr Relat Cancer. 2016;23:609-23 pubmed publisher
  13. Sun L, Zhang H, Bi L, Shi Y, Xing C, Tang L, et al. Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice. Am J Transl Res. 2016;8:2011-21 pubmed
  14. Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis E, et al. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells. PLoS ONE. 2016;11:e0156994 pubmed publisher
  15. Bachegowda L, Morrone K, Winski S, Mantzaris I, Bartenstein M, Ramachandra N, et al. Pexmetinib: A Novel Dual Inhibitor of Tie2 and p38 MAPK with Efficacy in Preclinical Models of Myelodysplastic Syndromes and Acute Myeloid Leukemia. Cancer Res. 2016;76:4841-4849 pubmed publisher
  16. Lombardi R, Chen S, Ruggiero A, Gurha P, Czernuszewicz G, Willerson J, et al. Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene. Circ Res. 2016;119:41-54 pubmed publisher
  17. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  18. Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
  19. Ohradanova Repic A, Machacek C, Fischer M, Stockinger H. Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology. 2016;5:e55 pubmed publisher
  20. Wang X, Dai Z, Wu X, Wang K, Wang X. Distinct RNA transcriptome patterns are potentially associated with angiogenesis in Tie2-expressing monocytes. Gene. 2016;580:1-7 pubmed publisher
  21. He Y, Wang C, Yu Y, Qian J, Song K, Sun Q, et al. Tie2-Expressing Monocytes Are Associated with Identification and Prognoses of Hepatitis B Virus Related Hepatocellular Carcinoma after Resection. PLoS ONE. 2015;10:e0143657 pubmed publisher
  22. Dokun A, Chen L, Okutsu M, Farber C, Hazarika S, Jones W, et al. ADAM12: a genetic modifier of preclinical peripheral arterial disease. Am J Physiol Heart Circ Physiol. 2015;309:H790-803 pubmed publisher
  23. Agarwal S, Loder S, Brownley C, Eboda O, Peterson J, Hayano S, et al. BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints. Dev Biol. 2015;400:202-9 pubmed publisher
  24. Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin β1 activation. Nat Commun. 2015;6:5962 pubmed publisher
  25. Besschetnova T, Ichimura T, Katebi N, St Croix B, Bonventre J, Olsen B. Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix Biol. 2015;42:56-73 pubmed publisher
  26. Rogacev K, Zawada A, Hundsdorfer J, Achenbach M, Held G, Fliser D, et al. Immunosuppression and monocyte subsets. Nephrol Dial Transplant. 2015;30:143-53 pubmed publisher
  27. Kawamura K, Takahashi T, Kanazawa M, Igarashi H, Nakada T, Nishizawa M, et al. Effects of angiopoietin-1 on hemorrhagic transformation and cerebral edema after tissue plasminogen activator treatment for ischemic stroke in rats. PLoS ONE. 2014;9:e98639 pubmed publisher
  28. Forgèt M, Voorhees J, Cole S, Dakhlallah D, Patterson I, Gross A, et al. Macrophage colony-stimulating factor augments Tie2-expressing monocyte differentiation, angiogenic function, and recruitment in a mouse model of breast cancer. PLoS ONE. 2014;9:e98623 pubmed publisher
  29. Fehring V, Schaeper U, Ahrens K, Santel A, Keil O, Eisermann M, et al. Delivery of therapeutic siRNA to the lung endothelium via novel Lipoplex formulation DACC. Mol Ther. 2014;22:811-20 pubmed publisher
  30. Soblet J, Limaye N, Uebelhoer M, Boon L, Vikkula M. Variable Somatic TIE2 Mutations in Half of Sporadic Venous Malformations. Mol Syndromol. 2013;4:179-83 pubmed publisher