This is a Validated Antibody Database (VAD) review about human separase, based on 5 published articles (read how Labome selects the articles), using separase antibody in all methods. It is aimed to help Labome visitors find the most suited separase antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
separase synonym: ESP1; SEPA; separin; caspase-like protein ESPL1; extra spindle pole bodies 1, separase; extra spindle pole bodies homolog 1; extra spindle poles like 1; extra spindle poles-like 1 protein; separin, cysteine protease

Abcam
mouse monoclonal (XJ11-1B12)
  • western blot; human; 1:500; fig 2
In order to assess the regulation of PLK1 and PCNT cleavage and mitotic exit via centriole separation, Abcam separase antibody (Abcam, ab16170) was used in western blot on human samples at 1:500 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (XJ11-1B12)
  • western blot; human; 1:500
In order to study the role of the pericentriolar material disassembly in centriole separation during mitotic exit, Abcam separase antibody (Abcam, ab16170) was used in western blot on human samples at 1:500. PLoS ONE (2015) ncbi
mouse monoclonal (XJ11-1B12)
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, Abcam separase antibody (Abcam, ab16170) was used in western blot on human samples . Biol Open (2015) ncbi
mouse monoclonal (XJ11-1B12)
  • western blot; human; 1:500
In order to study the role of separase at centrosomes, Abcam separase antibody (Abcam, ab16170) was used in western blot on human samples at 1:500. PLoS Genet (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500
In order to study the role of separase at centrosomes, Abcam separase antibody (Abcam, ab3762) was used in immunocytochemistry on human samples at 1:500. PLoS Genet (2014) ncbi
mouse monoclonal (XJ11-1B12)
  • western blot; human
In order to characterize securin isoforms and their role in separase regulation, Abcam separase antibody (Abcam, ab16170) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
Articles Reviewed
  1. Kim J, Lee K, Rhee K. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun. 2015;6:10076 pubmed publisher
  2. Seo M, Jang W, Rhee K. Integrity of the Pericentriolar Material Is Essential for Maintaining Centriole Association during M Phase. PLoS ONE. 2015;10:e0138905 pubmed publisher
  3. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  4. Agircan F, Schiebel E. Sensors at centrosomes reveal determinants of local separase activity. PLoS Genet. 2014;10:e1004672 pubmed publisher
  5. Han X, Poon R. Critical differences between isoforms of securin reveal mechanisms of separase regulation. Mol Cell Biol. 2013;33:3400-15 pubmed publisher