This is a Validated Antibody Database (VAD) review about human prolactin receptor, based on 28 published articles (read how Labome selects the articles), using prolactin receptor antibody in all methods. It is aimed to help Labome visitors find the most suited prolactin receptor antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
prolactin receptor synonym: HPRL; MFAB; RI-PRLR; hPRLrI

Invitrogen
mouse monoclonal (1A2B1)
  • western blot; human; loading ...; fig 3a
In order to compare HER2 and prolactin receptor trafficking, Invitrogen prolactin receptor antibody (Life Technologies, 35-9200) was used in western blot on human samples (fig 3a). Mol Cancer Ther (2017) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry; human; loading ...; fig 1d
In order to assess the expression of prolactin by synovial macrophages and determine if prolactin alters macrophage activation, Invitrogen prolactin receptor antibody (Invitrogen, 1A2B1) was used in immunohistochemistry on human samples (fig 1d). Rheumatology (Oxford) (2016) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
In order to identify a prolactin receptor mutation in 95 cases of multiple breast fibroadenomas, Invitrogen prolactin receptor antibody (Invitrogen, 1A2B1) was used in immunohistochemistry - paraffin section on human samples (fig 1a). J Clin Endocrinol Metab (2016) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; loading ...; fig 1g
In order to explore the extracellular matrix features that control prolactin signals, Invitrogen prolactin receptor antibody (Invitrogen, 35-9200) was used in western blot on human samples (fig 1g). Oncotarget (2016) ncbi
mouse monoclonal (B6.2)
  • immunohistochemistry; human; 1:200; fig 4
  • western blot; human; 1:2000; fig 4
In order to analyze a case report involving pineal diffuse large B-cell lymphoma concomitant with pituitary prolactinoma and a possible correlation between two pathologies, Invitrogen prolactin receptor antibody (Thermo Fisher Scientific, MA5-11955) was used in immunohistochemistry on human samples at 1:200 (fig 4) and in western blot on human samples at 1:2000 (fig 4). Medicine (Baltimore) (2016) ncbi
mouse monoclonal (1A2B1)
  • flow cytometry; human; loading ...; fig 2d
In order to examine the relationship between prolactin and T regulatory cells in patients with systemic lupus erythematosus, Invitrogen prolactin receptor antibody (Invitrogen, 1A2B1) was used in flow cytometry on human samples (fig 2d). Medicine (Baltimore) (2016) ncbi
mouse monoclonal (1A2B1)
  • western blot; mouse; fig 1
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
  • immunocytochemistry; human; fig 3
In order to study lymphangioleiomyomatosis and the role of prolactin receptors, Invitrogen prolactin receptor antibody (Invitrogen, 1A2B1) was used in western blot on mouse samples (fig 1), in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4) and in immunocytochemistry on human samples (fig 3). PLoS ONE (2016) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry; human; 1:100; loading ...; fig 1a
In order to test if prolactin alters the secondary tumor bone microenvironment, Invitrogen prolactin receptor antibody (Life Technologies, 1A2B1) was used in immunohistochemistry on human samples at 1:100 (fig 1a). J Natl Cancer Inst (2016) ncbi
mouse monoclonal (U5)
  • immunocytochemistry; bovine; 1:50; fig 5
In order to study the age-related effects of prolactin and growth hormone on bovine oocytes, Invitrogen prolactin receptor antibody (Thermo Scientific, MA1-610) was used in immunocytochemistry on bovine samples at 1:50 (fig 5). Front Genet (2015) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; 1:1000; fig  3
Invitrogen prolactin receptor antibody (Zymed Laboratories, 1A2B1) was used in western blot on human samples at 1:1000 (fig  3). J Biol Chem (2015) ncbi
mouse monoclonal (1A2B1)
  • western blot; human
In order to use collagen matrices to investigate the effects of 17beta-estradiol activity and PRL/E2 interactions in breast cancer cell lines in vitro, Invitrogen prolactin receptor antibody (Invitrogen, 35-C9200) was used in western blot on human samples . PLoS ONE (2015) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; 1:1000
In order to study the role of prolactin and its receptors in breast cancer, Invitrogen prolactin receptor antibody (Zymed, 1A2B1) was used in western blot on human samples at 1:1000. Cancer Lett (2015) ncbi
mouse monoclonal (U5)
  • immunocytochemistry; bovine; 1:50
In order to characterize pathways of PRL action on bovine oocytes, Invitrogen prolactin receptor antibody (Thermo Scientific, MA1-610) was used in immunocytochemistry on bovine samples at 1:50. Theriogenology (2014) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; fig 4
In order to investigate the role of prolactin in breast cancer, Invitrogen prolactin receptor antibody (Zymed, 1A2B1) was used in western blot on human samples (fig 4). Breast Cancer Res Treat (2013) ncbi
mouse monoclonal (1A2B1)
  • western blot; human
In order to study the involvement of collagen matrices in tumorigenic prolactin signaling in breast cancer cells, Invitrogen prolactin receptor antibody (Invitrogen, 35-9200) was used in western blot on human samples . J Biol Chem (2013) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry; human; fig 6
In order to investigate the functions of prolactin in endothelial cells and its involvement in pathological angiogenesis, Invitrogen prolactin receptor antibody (Zymed, 1A2B1) was used in immunohistochemistry on human samples (fig 6). J Cell Mol Med (2012) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; 1:1000; fig 1
In order to investigate variably-spliced prolactin receptors, Invitrogen prolactin receptor antibody (Zymed, 35-9200) was used in western blot on human samples at 1:1000 (fig 1). Cancer Lett (2011) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry; human; 1:500; fig 2
In order to examine the rotation model of the human prolactin receptor, Invitrogen prolactin receptor antibody (Invitrogen, clone 1A2B1) was used in immunohistochemistry on human samples at 1:500 (fig 2). Biochemistry (2011) ncbi
mouse monoclonal (1A2B1)
  • immunohistochemistry - paraffin section; human; 1:500; fig 2
In order to investigate the localization of the prolactin receptor in atherosclerotic plaques, Invitrogen prolactin receptor antibody (Zymed, 1A2B1) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 2). J Endocrinol (2011) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; fig 17.2
In order to discuss how prolactin receptor variants were discovered as contributors of breast cancer, Invitrogen prolactin receptor antibody (Zymed, 35- 9200) was used in western blot on human samples (fig 17.2). Methods Enzymol (2010) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; fig 1
In order to study the interaction between prolactin and oestrogen in breast cancer cells, Invitrogen prolactin receptor antibody (Zymed, 1A2B1) was used in western blot on human samples (fig 1). Endocr Relat Cancer (2010) ncbi
mouse monoclonal (1A2B1)
  • western blot; human
In order to elucidate the mechanism of cross-talk between PRL and IGF-I in breast cancer cells, Invitrogen prolactin receptor antibody (Zymed Laboratories, 35-9200) was used in western blot on human samples . J Biol Chem (2010) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; fig 3
In order to study the mechanisms of PRL receptor proteolysis, Invitrogen prolactin receptor antibody (Invitrogen, 1A2B1) was used in western blot on human samples (fig 3). Mol Cell Biol (2008) ncbi
mouse monoclonal (1A2B1)
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 3
In order to examine the effects of S179D prolactin on endothelial cells, Invitrogen prolactin receptor antibody (Zymed, 35-9200) was used in immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig 3). Endocr Relat Cancer (2006) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; 1:2000; fig 5
In order to study endogenous prolactin, Invitrogen prolactin receptor antibody (Zymed, 35-9200) was used in western blot on human samples at 1:2000 (fig 5). J Steroid Biochem Mol Biol (2004) ncbi
mouse monoclonal (1A2B1)
  • western blot; human; 1:2000; fig 4
In order to test if a mimic of phosphorylated human prolactin, S179D-PRL, initiates signaling in mammary tumor cells alone and in combination with unmodified prolactin, Invitrogen prolactin receptor antibody (Zymed, 35-9200) was used in western blot on human samples at 1:2000 (fig 4). Endocrinology (2003) ncbi
Abcam
domestic rabbit monoclonal (EPR7184(2))
  • immunohistochemistry; rat; 1:100; loading ...; fig 3a
Abcam prolactin receptor antibody (Abcam, ab170935) was used in immunohistochemistry on rat samples at 1:100 (fig 3a). J Reprod Dev (2020) ncbi
mouse monoclonal (U5)
  • western blot; human; 1:1000
Abcam prolactin receptor antibody (Abcam, U5) was used in western blot on human samples at 1:1000. J Biol Chem (2015) ncbi
mouse monoclonal (U5)
  • immunohistochemistry - paraffin section; rat; 1:80; loading ...; fig 2b
Abcam prolactin receptor antibody (Abcam, ab2772) was used in immunohistochemistry - paraffin section on rat samples at 1:80 (fig 2b). Chin J Integr Med (2015) ncbi
Articles Reviewed
  1. Horiguchi K, Yoshida S, Tsukada T, Nakakura T, Fujiwara K, Hasegawa R, et al. Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells. J Reprod Dev. 2020;66:515-522 pubmed publisher
  2. Andreev J, Thambi N, Perez Bay A, Delfino F, Martin J, Kelly M, et al. Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs. Mol Cancer Ther. 2017;16:681-693 pubmed publisher
  3. Tang M, Reedquist K, Garcia S, Fernandez B, Codullo V, Vieira Sousa E, et al. The prolactin receptor is expressed in rheumatoid arthritis and psoriatic arthritis synovial tissue and contributes to macrophage activation. Rheumatology (Oxford). 2016;55:2248-2259 pubmed
  4. Chakhtoura Z, Laki F, Bernadet M, Cherifi I, Chiche A, Pigat N, et al. Gain-of-function Prolactin Receptor Variants Are Not Associated With Breast Cancer and Multiple Fibroadenoma Risk. J Clin Endocrinol Metab. 2016;101:4449-4460 pubmed
  5. Barcus C, Keely P, Eliceiri K, Schuler L. Prolactin signaling through focal adhesion complexes is amplified by stiff extracellular matrices in breast cancer cells. Oncotarget. 2016;7:48093-48106 pubmed publisher
  6. Kim Y, Kim H, Yang D, Jung S, Noh M, Lee J, et al. Pineal Diffuse Large B-Cell Lymphoma Concomitant With Pituitary Prolactinoma: Possible Correlation Between 2 Distinguished Pathologies: A Case Report. Medicine (Baltimore). 2016;95:e2923 pubmed publisher
  7. Legorreta Haquet M, Chávez Rueda K, Chávez Sánchez L, Cervera Castillo H, Zenteno Galindo E, Barile Fabris L, et al. Function of Treg Cells Decreased in Patients With Systemic Lupus Erythematosus Due To the Effect of Prolactin. Medicine (Baltimore). 2016;95:e2384 pubmed publisher
  8. Alkharusi A, Lesma E, Ancona S, Chiaramonte E, Nystrom T, Gorio A, et al. Role of Prolactin Receptors in Lymphangioleiomyomatosis. PLoS ONE. 2016;11:e0146653 pubmed publisher
  9. Sutherland A, Forsyth A, Cong Y, Grant L, Juan T, Lee J, et al. The Role of Prolactin in Bone Metastasis and Breast Cancer Cell-Mediated Osteoclast Differentiation. J Natl Cancer Inst. 2016;108: pubmed publisher
  10. Lebedeva I, Singina G, Lopukhov A, Shedova E, Zinovieva N. Prolactin and growth hormone affect metaphase-II chromosomes in aging oocytes via cumulus cells using similar signaling pathways. Front Genet. 2015;6:274 pubmed publisher
  11. Zhang C, Nygaard M, Haxholm G, Boutillon F, Bernadet M, Hoos S, et al. A Residue Quartet in the Extracellular Domain of the Prolactin Receptor Selectively Controls Mitogen-activated Protein Kinase Signaling. J Biol Chem. 2015;290:11890-904 pubmed publisher
  12. Barcus C, Holt E, Keely P, Eliceiri K, Schuler L. Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells. PLoS ONE. 2015;10:e0116891 pubmed publisher
  13. Huang K, Tan D, Chen K, Walker A. Blockade of estrogen-stimulated proliferation by a constitutively-active prolactin receptor having lower expression in invasive ductal carcinoma. Cancer Lett. 2015;358:152-60 pubmed publisher
  14. Lebedeva I, Singina G, Volkova N, Vejlsted M, Zinovieva N, Schmidt M. Prolactin affects bovine oocytes through direct and cumulus-mediated pathways. Theriogenology. 2014;82:1154-64 pubmed publisher
  15. Xiong F, Gui J, Yang W, Li J, Huang G. Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction. Chin J Integr Med. 2015;21:58-66 pubmed publisher
  16. Nitze L, Galsgaard E, Din N, Lund V, Rasmussen B, Berchtold M, et al. Reevaluation of the proposed autocrine proliferative function of prolactin in breast cancer. Breast Cancer Res Treat. 2013;142:31-44 pubmed publisher
  17. Barcus C, Keely P, Eliceiri K, Schuler L. Stiff collagen matrices increase tumorigenic prolactin signaling in breast cancer cells. J Biol Chem. 2013;288:12722-32 pubmed publisher
  18. Reuwer A, Nowak Sliwinska P, Mans L, van der Loos C, von der Thüsen J, Twickler M, et al. Functional consequences of prolactin signalling in endothelial cells: a potential link with angiogenesis in pathophysiology?. J Cell Mol Med. 2012;16:2035-48 pubmed publisher
  19. Tan D, Chen K, Khoo T, Walker A. Prolactin increases survival and migration of ovarian cancer cells: importance of prolactin receptor type and therapeutic potential of S179D and G129R receptor antagonists. Cancer Lett. 2011;310:101-8 pubmed publisher
  20. Liu W, Brooks C. Functional impact of manipulation on the relative orientation of human prolactin receptor domains. Biochemistry. 2011;50:5333-44 pubmed publisher
  21. Reuwer A, van Eijk M, Houttuijn Bloemendaal F, van der Loos C, Claessen N, Teeling P, et al. The prolactin receptor is expressed in macrophages within human carotid atherosclerotic plaques: a role for prolactin in atherogenesis?. J Endocrinol. 2011;208:107-17 pubmed publisher
  22. Goffin V, Bogorad R, Touraine P. Identification of gain-of-function variants of the human prolactin receptor. Methods Enzymol. 2010;484:329-55 pubmed publisher
  23. Rasmussen L, Frederiksen K, Din N, Galsgaard E, Christensen L, Berchtold M, et al. Prolactin and oestrogen synergistically regulate gene expression and proliferation of breast cancer cells. Endocr Relat Cancer. 2010;17:809-22 pubmed publisher
  24. Carver K, Piazza T, Schuler L. Prolactin enhances insulin-like growth factor I receptor phosphorylation by decreasing its association with the tyrosine phosphatase SHP-2 in MCF-7 breast cancer cells. J Biol Chem. 2010;285:8003-12 pubmed publisher
  25. Varghese B, Barriere H, Carbone C, Banerjee A, Swaminathan G, Plotnikov A, et al. Polyubiquitination of prolactin receptor stimulates its internalization, postinternalization sorting, and degradation via the lysosomal pathway. Mol Cell Biol. 2008;28:5275-87 pubmed publisher
  26. Ueda E, Ozerdem U, Chen Y, Yao M, Huang K, Sun H, et al. A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone. Endocr Relat Cancer. 2006;13:95-111 pubmed
  27. Gutzman J, Miller K, Schuler L. Endogenous human prolactin and not exogenous human prolactin induces estrogen receptor alpha and prolactin receptor expression and increases estrogen responsiveness in breast cancer cells. J Steroid Biochem Mol Biol. 2004;88:69-77 pubmed
  28. Schroeder M, Brockman J, Walker A, Schuler L. Inhibition of prolactin (PRL)-induced proliferative signals in breast cancer cells by a molecular mimic of phosphorylated PRL, S179D-PRL. Endocrinology. 2003;144:5300-7 pubmed