This is a Validated Antibody Database (VAD) review about human prion protein, based on 21 published articles (read how Labome selects the articles), using prion protein antibody in all methods. It is aimed to help Labome visitors find the most suited prion protein antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
prion protein synonym: ASCR; AltPrP; CD230; CJD; GSS; KURU; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; p27-30

Knockout validation
Bertin Pharma
mouse monoclonal (Sha 31)
  • western blot knockout validation; mouse; 1:2000; fig 1
In order to study the cause of diverse proteome shifts in cell models that escape detection in brain tissue with prion protein deficiency, Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1). PLoS ONE (2016) ncbi
Bertin Pharma
mouse monoclonal (Sha 31)
  • western blot knockout validation; mouse; 1:5000; fig 2a
Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot knockout validation on mouse samples at 1:5000 (fig 2a). PLoS ONE (2014) ncbi
BioLegend
mouse monoclonal (6D11)
  • western blot; mouse; 1:5000; loading ...; fig 3
BioLegend prion protein antibody (Biolegend, 6D11) was used in western blot on mouse samples at 1:5000 (fig 3). PLoS ONE (2022) ncbi
mouse monoclonal (6D11)
  • immunohistochemistry; mouse; 1:200
BioLegend prion protein antibody (BioLegend, 808,001) was used in immunohistochemistry on mouse samples at 1:200. Acta Neuropathol Commun (2021) ncbi
mouse monoclonal (3F4)
  • immunohistochemistry; human; loading ...; fig 6c
  • western blot; human; 1:500; loading ...; fig 6b
BioLegend prion protein antibody (Signet Laboratories, 3F4) was used in immunohistochemistry on human samples (fig 6c) and in western blot on human samples at 1:500 (fig 6b). Exp Eye Res (2018) ncbi
mouse monoclonal (6D11)
  • immunocytochemistry; mouse; loading ...; fig 1d
  • western blot; mouse; 1:1000; loading ...; fig 1b
BioLegend prion protein antibody (Covance, 6D11) was used in immunocytochemistry on mouse samples (fig 1d) and in western blot on mouse samples at 1:1000 (fig 1b). J Virol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (6D11)
  • western blot; African green monkey; loading ...; fig 1
  • western blot; bovine; loading ...; fig 1
  • western blot; human; loading ...; fig 1
Santa Cruz Biotechnology prion protein antibody (Santa Cruz, 6D11) was used in western blot on African green monkey samples (fig 1), in western blot on bovine samples (fig 1) and in western blot on human samples (fig 1). Pathogens (2022) ncbi
mouse monoclonal (6D11)
  • western blot; mouse; fig 1a
Santa Cruz Biotechnology prion protein antibody (Santa Cruz Biotechnology, 6D11) was used in western blot on mouse samples (fig 1a). J Biol Chem (2019) ncbi
mouse monoclonal (6D11)
  • immunohistochemistry - paraffin section; mouse; fig 5
  • western blot; mouse; fig 3
Santa Cruz Biotechnology prion protein antibody (Santa Cruz, sc-58581) was used in immunohistochemistry - paraffin section on mouse samples (fig 5) and in western blot on mouse samples (fig 3). Int J Mol Med (2016) ncbi
mouse monoclonal (6D11)
  • immunohistochemistry - paraffin section; mouse; 1:100
In order to investigate the spread of neuronal pathology, Santa Cruz Biotechnology prion protein antibody (Santa Cruz, 6D11) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Neuropathol Appl Neurobiol (2015) ncbi
mouse monoclonal (6D11)
  • other; mouse; 1:50; loading ...; fig 2a
Santa Cruz Biotechnology prion protein antibody (Santa Cruz, sc-58581) was used in other on mouse samples at 1:50 (fig 2a). Cell Death Differ (2015) ncbi
mouse monoclonal (6D11)
  • immunocytochemistry; hamsters; 1:100
  • western blot; hamsters
Santa Cruz Biotechnology prion protein antibody (SantaCruz, sc-58581) was used in immunocytochemistry on hamsters samples at 1:100 and in western blot on hamsters samples . J Mol Neurosci (2015) ncbi
Abcam
mouse monoclonal (F89/160.1.5)
  • immunohistochemistry - paraffin section; domestic goat; 1:2000; loading ...; fig 4a-4e
Abcam prion protein antibody (Abcam, F89) was used in immunohistochemistry - paraffin section on domestic goat samples at 1:2000 (fig 4a-4e). Vet Res (2020) ncbi
mouse monoclonal (8H4)
  • immunohistochemistry - free floating section; mouse; 1:1000; loading ...; fig 1e
Abcam prion protein antibody (Abcam, ab61409) was used in immunohistochemistry - free floating section on mouse samples at 1:1000 (fig 1e). J Neurosci (2017) ncbi
domestic rabbit monoclonal (EP1802Y)
  • western blot; human; 1:500; loading ...; fig 2e
Abcam prion protein antibody (Abcam, ab52604) was used in western blot on human samples at 1:500 (fig 2e). Sci Rep (2017) ncbi
domestic rabbit monoclonal (EP1802Y)
  • western blot; human; 1:5000
Abcam prion protein antibody (Abcam, ab52604) was used in western blot on human samples at 1:5000. Biochim Biophys Acta (2014) ncbi
Invitrogen
mouse monoclonal (4D5)
  • flow cytometry; human; 1:50; fig 3a, 3b
Invitrogen prion protein antibody (eBioscience/Thermo, 12-9230-42) was used in flow cytometry on human samples at 1:50 (fig 3a, 3b). Stem Cells (2019) ncbi
mouse monoclonal (4D5)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, Invitrogen prion protein antibody (e- Bioscienc e, 12-9230-73) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Bertin Pharma
mouse monoclonal (Sha 31)
  • western blot; mouse; 1:2000; loading ...; fig 2c
In order to examine if prion protein and ZIP6 are critical during epithelial-to-mesenchymal transition, Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot on mouse samples at 1:2000 (fig 2c). Sci Rep (2017) ncbi
mouse monoclonal (Sha 31)
  • immunoprecipitation; mouse; fig s3
In order to investigate the effect of prion protein absence on copper and iron metabolism during development and adulthood, Bertin Pharma prion protein antibody (BertinPharma, A03213) was used in immunoprecipitation on mouse samples (fig s3). Front Neurosci (2016) ncbi
mouse monoclonal (Sha 31)
  • western blot knockout validation; mouse; 1:2000; fig 1
In order to study the cause of diverse proteome shifts in cell models that escape detection in brain tissue with prion protein deficiency, Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (Sha 31)
  • western blot; mouse; 1:2000; fig 1
Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot on mouse samples at 1:2000 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (Sha 31)
  • western blot knockout validation; mouse; 1:5000; fig 2a
Bertin Pharma prion protein antibody (Bertin Pharma, A03213) was used in western blot knockout validation on mouse samples at 1:5000 (fig 2a). PLoS ONE (2014) ncbi
Cayman Chemical
mouse monoclonal (SAF 32)
  • western blot; mouse; 1:200; loading ...; fig 1a
Cayman Chemical prion protein antibody (Cayman, 189720) was used in western blot on mouse samples at 1:200 (fig 1a). J Neurosci (2017) ncbi
Articles Reviewed
  1. Piccardo P, Cervenak J, Goldmann W, Stewart P, Pomeroy K, Gregori L, et al. Experimental Bovine Spongiform Encephalopathy in Squirrel Monkeys: The Same Complex Proteinopathy Appearing after Very Different Incubation Times. Pathogens. 2022;11: pubmed publisher
  2. Ward A, Jessop F, Faris R, Shoup D, Bosio C, Peterson K, et al. Lack of the immune adaptor molecule SARM1 accelerates disease in prion infected mice and is associated with increased mitochondrial respiration and decreased expression of NRF2. PLoS ONE. 2022;17:e0267720 pubmed publisher
  3. Pankiewicz J, Lizińczyk A, Franco L, Díaz J, Martá Ariza M, Sadowski M. Absence of Apolipoprotein E is associated with exacerbation of prion pathology and promotes microglial neurodegenerative phenotype. Acta Neuropathol Commun. 2021;9:157 pubmed publisher
  4. Salvesen Ø, Espenes A, Reiten M, Vuong T, Malachin G, Tran L, et al. Goats naturally devoid of PrPC are resistant to scrapie. Vet Res. 2020;51:1 pubmed publisher
  5. Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett P, et al. Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells. Stem Cells. 2019;37:1293-1306 pubmed publisher
  6. Silverman J, Christy D, Shyu C, Moon K, Fernando S, Gidden Z, et al. CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)G93A ALS mice originate from astrocytes and neurons and carry misfolded SOD1. J Biol Chem. 2019;294:3744-3759 pubmed publisher
  7. Ashok A, Karmakar S, Chandel R, Ravikumar R, Dalal S, Kong Q, et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission. Exp Eye Res. 2018;175:1-13 pubmed publisher
  8. Salazar S, Gallardo C, Kaufman A, Herber C, Haas L, Robinson S, et al. Conditional Deletion of Prnp Rescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease. J Neurosci. 2017;37:9207-9221 pubmed publisher
  9. Déry M, LeBlanc A. Luman contributes to brefeldin A-induced prion protein gene expression by interacting with the ERSE26 element. Sci Rep. 2017;7:42285 pubmed publisher
  10. Brethour D, Mehrabian M, Williams D, Wang X, Ghodrati F, Ehsani S, et al. A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition. Sci Rep. 2017;7:40313 pubmed publisher
  11. Marshall K, Hughson A, Vascellari S, Priola S, Sakudo A, Onodera T, et al. PrP Knockout Cells Expressing Transmembrane PrP Resist Prion Infection. J Virol. 2017;91: pubmed publisher
  12. Gasperini L, Meneghetti E, Legname G, Benetti F. In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. Front Neurosci. 2016;10:437 pubmed
  13. Mehrabian M, Brethour D, Williams D, Wang H, Arnould H, Schneider B, et al. Prion Protein Deficiency Causes Diverse Proteome Shifts in Cell Models That Escape Detection in Brain Tissue. PLoS ONE. 2016;11:e0156779 pubmed publisher
  14. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  15. Xiao K, Zhang B, Zhang X, Wang J, Chen C, Chen L, et al. Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c. Int J Mol Med. 2016;37:716-26 pubmed publisher
  16. Mehrabian M, Brethour D, Wang H, Xi Z, Rogaeva E, Schmitt Ulms G. The Prion Protein Controls Polysialylation of Neural Cell Adhesion Molecule 1 during Cellular Morphogenesis. PLoS ONE. 2015;10:e0133741 pubmed publisher
  17. Reis R, Hennessy E, Murray C, Griffin Ã, Cunningham C. At the centre of neuronal, synaptic and axonal pathology in murine prion disease: degeneration of neuroanatomically linked thalamic and brainstem nuclei. Neuropathol Appl Neurobiol. 2015;41:780-97 pubmed publisher
  18. Woo J, Zhao X, Khan H, Penn C, Wang X, Joly Amado A, et al. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell Death Differ. 2015;22:921-34 pubmed publisher
  19. Mehrabian M, Brethour D, MacIsaac S, Kim J, Gunawardana C, Wang H, et al. CRISPR-Cas9-based knockout of the prion protein and its effect on the proteome. PLoS ONE. 2014;9:e114594 pubmed publisher
  20. Wang J, Zhang J, Shi Q, Zhang B, Chen C, Chen L, et al. Scrapie infection in experimental rodents and SMB-S15 cells decreased the brain endogenous levels and activities of Sirt1. J Mol Neurosci. 2015;55:1022-30 pubmed publisher
  21. Feng B, Wang Z, Liu T, Jin R, Wang S, Wang W, et al. Methionine oxidation accelerates the aggregation and enhances the neurotoxicity of the D178N variant of the human prion protein. Biochim Biophys Acta. 2014;1842:2345-56 pubmed publisher