This is a Validated Antibody Database (VAD) review about human phospholamban, based on 26 published articles (read how Labome selects the articles), using phospholamban antibody in all methods. It is aimed to help Labome visitors find the most suited phospholamban antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
phospholamban synonym: CMD1P; CMH18; PLB

Invitrogen
mouse monoclonal (2D12)
  • western blot; mouse; loading ...; fig 4i
Invitrogen phospholamban antibody (Thermo Fisher Scientific, 2D12) was used in western blot on mouse samples (fig 4i). Sci Adv (2022) ncbi
domestic rabbit polyclonal
  • western blot; African green monkey; 1:1000; loading ...; fig s11
Invitrogen phospholamban antibody (Thermo Fisher, PA5-82945) was used in western blot on African green monkey samples at 1:1000 (fig s11). Commun Biol (2021) ncbi
mouse monoclonal (2D12)
  • western blot; domestic rabbit; 1:2000; loading ...; fig 5
In order to report the effects of heart failure on the electrical activity of sinoatrial nodes with and without pharmacological interventions, Invitrogen phospholamban antibody (Thermo Fisher, MA3-922) was used in western blot on domestic rabbit samples at 1:2000 (fig 5). Exp Ther Med (2017) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; loading ...; fig 1b
In order to report the physiological role of sarcolipin upregulation in muscle myopathy, Invitrogen phospholamban antibody (Pierce Antibodies, 2D12) was used in western blot on mouse samples (fig 1b). PLoS ONE (2017) ncbi
mouse monoclonal (2D12)
  • western blot; rat; fig 8b
In order to analyze cardiomyocyte function in dependence of TRPC4 splice variant expression., Invitrogen phospholamban antibody (Millipore, MA3-922) was used in western blot on rat samples (fig 8b). PLoS ONE (2016) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:1000; loading ...; fig 2c
In order to evaluate the impacts of high fat diet on the insulin-adrenergic receptor signal network in hearts, Invitrogen phospholamban antibody (Affinity Bioreagent, MA3-922) was used in western blot on mouse samples at 1:1000 (fig 2c). J Physiol (2017) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:2000; fig 1
In order to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban, Invitrogen phospholamban antibody (Pierce Antibodies, 2D12) was used in western blot on mouse samples at 1:2000 (fig 1). Brain Behav (2016) ncbi
mouse monoclonal (2D12)
  • western blot; rat; 1:5000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, Invitrogen phospholamban antibody (Thermo Fisher Scientific, MA3-922) was used in western blot on rat samples at 1:5000 (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:5000; loading ...; fig s9a
In order to elucidate how DWORF activates the SERCA pump, Invitrogen phospholamban antibody (Pierce, 2D12) was used in western blot on mouse samples at 1:5000 (fig s9a). Science (2016) ncbi
domestic goat polyclonal
  • western blot; human; fig 2
In order to assess the effect of intensive training in combination with marked reduction in training volume on phospholemman expression and phosphorylation at rest and during exercise, Invitrogen phospholamban antibody (Pierce-ThermoScientific, PA5-19351) was used in western blot on human samples (fig 2). Am J Physiol Regul Integr Comp Physiol (2016) ncbi
mouse monoclonal (2D12)
  • western blot; human
  • western blot; mouse
In order to study SERCA dysfunction in mice overexpressing phospholamban, Invitrogen phospholamban antibody (Pierce Antibodies, 2D12) was used in western blot on human samples and in western blot on mouse samples . Dis Model Mech (2015) ncbi
mouse monoclonal (2D12)
  • western blot; rat; fig 5
In order to determine the relationship between ACE and ACE2 activity, left ventricular function, and gender in spontaneously hypertensive rats, Invitrogen phospholamban antibody (Affinity BioReagents, MA3-922) was used in western blot on rat samples (fig 5). PLoS ONE (2015) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:20,000
In order to study the relationship between the E2F pathway, beta2-adrenergic/PKA/PDE4D, and ERK/c-Src axis during the pathological hypertrophic growth response, Invitrogen phospholamban antibody (Affinity Bioreagents, MA3-922) was used in western blot on mouse samples at 1:20,000. J Mol Cell Cardiol (2015) ncbi
mouse monoclonal (2D12)
  • western blot; domestic rabbit
In order to study the effect of progesterone on SERCA2a regulation in isolated cardiomyocytes, Invitrogen phospholamban antibody (Thermo Scientific, MA3-922) was used in western blot on domestic rabbit samples . Am J Physiol Cell Physiol (2014) ncbi
mouse monoclonal (2D12)
  • western blot; rat; 1:5000; fig 5
In order to learn that imbalance between myocardial phospholamban dephosphorylation and phosphorylation is not caused by obesity, Invitrogen phospholamban antibody (Thermo Scientific, MA3-922) was used in western blot on rat samples at 1:5000 (fig 5). Arq Bras Cardiol (2014) ncbi
mouse monoclonal (2D12)
  • western blot; mouse
In order to describe a protocol to determine the affinity of Ca(2+) to the Ca(2+) pump in crude homogenates of mouse heart., Invitrogen phospholamban antibody (Affinity BioReagents, MA3-922) was used in western blot on mouse samples . Cold Spring Harb Protoc (2014) ncbi
mouse monoclonal (2D12)
  • immunohistochemistry - frozen section; human; 1:250; fig 4
  • immunoprecipitation; human; fig 6
  • western blot; human; 1:2000; fig 3
In order to determine if sarcolipin and phospholamban regulate SERCA1a, SERCA2a, or both in skeletal muscle, Invitrogen phospholamban antibody (Pierce, 2D12) was used in immunohistochemistry - frozen section on human samples at 1:250 (fig 4), in immunoprecipitation on human samples (fig 6) and in western blot on human samples at 1:2000 (fig 3). PLoS ONE (2013) ncbi
mouse monoclonal (2D12)
  • western blot; human
In order to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure, Invitrogen phospholamban antibody (Pierce, 2D12) was used in western blot on human samples . J Card Fail (2012) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig 1e
Novus Biologicals phospholamban antibody (Novus Biologicals, NBP2-19807) was used in immunocytochemistry on human samples at 1:100 (fig 1e). EMBO Mol Med (2021) ncbi
Badrilla
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3d
Badrilla phospholamban antibody (Badrilla, A010-13) was used in western blot on human samples (fig 3d). Front Pharmacol (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3d
Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on human samples (fig 3d). Front Pharmacol (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:5000; loading ...; fig 5a
Badrilla phospholamban antibody (Badrilla, A010-13) was used in western blot on mouse samples at 1:5000 (fig 5a). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:5000; loading ...; fig 5a
Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on mouse samples at 1:5000 (fig 5a). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; domestic rabbit; 1:5000; loading ...; fig 5
In order to report the effects of heart failure on the electrical activity of sinoatrial nodes with and without pharmacological interventions, Badrilla phospholamban antibody (Badrilla, A010-13) was used in western blot on domestic rabbit samples at 1:5000 (fig 5). Exp Ther Med (2017) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:5000; loading ...; fig 2c
Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on rat samples at 1:5000 (fig 2c). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 6c
In order to test if BGP-15 ameliorate established disease in a mouse model of Duchenne muscular dystrophy, Badrilla phospholamban antibody (Badrilla, AO10-13) was used in western blot on mouse samples (fig 6c). Am J Pathol (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:5000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, Badrilla phospholamban antibody (Badrilla, A010-13) was used in western blot on rat samples at 1:5000 (fig s1). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:5000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on rat samples at 1:5000 (fig s1). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 7d
In order to study the role of angiotensin receptor-associated protein Atrap on cardiac Ca2+-ATPase SERCA2a, Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on mouse samples (fig 7d). Cardiovasc Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 3
In order to maintain cardiac function through a heterozygous deletion of sarcolipin, Badrilla phospholamban antibody (Badrilla, A010-12) was used in western blot on mouse samples (fig 3). Am J Physiol Heart Circ Physiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 3
In order to maintain cardiac function through a heterozygous deletion of sarcolipin, Badrilla phospholamban antibody (Badrilla, A010-13) was used in western blot on mouse samples (fig 3). Am J Physiol Heart Circ Physiol (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig s2h-j
Cell Signaling Technology phospholamban antibody (Cell Signaling, 8496) was used in western blot on mouse samples at 1:2000 (fig s2h-j). Nature (2020) ncbi
Articles Reviewed
  1. Contreras W, Wieseh xf6 fer C, Schreier D, Leinung N, Peche P, Wennemuth G, et al. C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly. Sci Adv. 2022;8:eabo6049 pubmed publisher
  2. Neumann J, Grobe J, Weisgut J, Schwelberger H, Fogel W, Marusakova M, et al. Histamine can be Formed and Degraded in the Human and Mouse Heart. Front Pharmacol. 2021;12:582916 pubmed publisher
  3. Cuello F, Knaust A, Saleem U, Loos M, Raabe J, Mosqueira D, et al. Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation. EMBO Mol Med. 2021;13:e13074 pubmed publisher
  4. Liu M, Li N, Qu C, Gao Y, Wu L, Hu L. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021;4:188 pubmed publisher
  5. Liu G, Papa A, Katchman A, Zakharov S, Roybal D, Hennessey J, et al. Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics. Nature. 2020;577:695-700 pubmed publisher
  6. Dai W, Laforest B, Tyan L, Shen K, Nadadur R, Alvarado F, et al. A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice. elife. 2019;8: pubmed publisher
  7. Chang S, Chuang H, Chen Y, Kao Y, Lin Y, Yeh Y, et al. Heart failure modulates electropharmacological characteristics of sinoatrial nodes. Exp Ther Med. 2017;13:771-779 pubmed publisher
  8. Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  9. Kirschmer N, Bandleon S, von Ehrlich Treuenstätt V, Hartmann S, Schaaf A, Lamprecht A, et al. TRPC4? and TRPC4? Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation. PLoS ONE. 2016;11:e0168446 pubmed publisher
  10. Fu Q, Hu Y, Wang Q, Liu Y, Li N, Xu B, et al. High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β2 -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts. J Physiol. 2017;595:1973-1986 pubmed publisher
  11. Scotcher J, Prysyazhna O, Boguslavskyi A, Kistamás K, Hadgraft N, Martin E, et al. Disulfide-activated protein kinase G I? regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. Nat Commun. 2016;7:13187 pubmed publisher
  12. Kennedy T, Swiderski K, Murphy K, Gehrig S, Curl C, Chandramouli C, et al. BGP-15 Improves Aspects of the Dystrophic Pathology in mdx and dko Mice with Differing Efficacies in Heart and Skeletal Muscle. Am J Pathol. 2016;186:3246-3260 pubmed publisher
  13. Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
  14. Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  15. Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach K, Grill A, et al. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a. Cardiovasc Res. 2016;110:359-70 pubmed publisher
  16. Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  17. Thomassen M, Gunnarsson T, Christensen P, Pavlovic D, Shattock M, Bangsbo J. Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. Am J Physiol Regul Integr Comp Physiol. 2016;310:R659-69 pubmed publisher
  18. Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, et al. Heterozygous deletion of sarcolipin maintains normal cardiac function. Am J Physiol Heart Circ Physiol. 2016;310:H92-103 pubmed publisher
  19. Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
  20. Dalpiaz P, Lamas A, Caliman I, Ribeiro R, Abreu G, Moyses M, et al. Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats. PLoS ONE. 2015;10:e0127515 pubmed publisher
  21. Major J, Salih M, Tuana B. Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium. J Mol Cell Cardiol. 2015;84:179-90 pubmed publisher
  22. Moshal K, Zhang Z, Roder K, Kim T, Cooper L, Patedakis Litvinov B, et al. Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes. Am J Physiol Cell Physiol. 2014;307:C1050-7 pubmed publisher
  23. Freire P, Alves C, Deus A, Leopoldo A, Leopoldo A, Silva D, et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol. 2014;103:41-50 pubmed
  24. Holemans T, Vandecaetsbeek I, Wuytack F, Vangheluwe P. Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart. Cold Spring Harb Protoc. 2014;2014:876-86 pubmed publisher
  25. Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
  26. Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher