This is a Validated Antibody Database (VAD) review about human perlecan, based on 26 published articles (read how Labome selects the articles), using perlecan antibody in all methods. It is aimed to help Labome visitors find the most suited perlecan antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
perlecan synonym: HSPG; PLC; PRCAN; SJA; SJS; SJS1

Invitrogen
brown rat monoclonal (A7L6)
  • immunohistochemistry; dogs; 1:1000; loading ...; fig s6a
Invitrogen perlecan antibody (Thermofisher, A7L6) was used in immunohistochemistry on dogs samples at 1:1000 (fig s6a). Science (2018) ncbi
mouse monoclonal (7B5)
  • immunohistochemistry - frozen section; human; 1:100; fig 4a
In order to characterize the effects of mild skin massage on cell behavior, Invitrogen perlecan antibody (Invitrogen, 13-4400) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 4a). PLoS ONE (2017) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 3a
In order to show that a defined cosmetic composition results in the production of sulfated glycosaminoglycans and may have anti-ageing benefits, Invitrogen perlecan antibody (Thermo Fisher, MA1-06821) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3a). Clin Cosmet Investig Dermatol (2016) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry; mouse; 1:100; fig S1G
In order to reveal that platelet-derived growth factor alpha and beta are coexpressed in the craniofacial mesenchyme of mid-gestation mouse embryos, Invitrogen perlecan antibody (Thermo Fisher Scientific, A7L6) was used in immunohistochemistry on mouse samples at 1:100 (fig S1G). Genes Dev (2016) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry; mouse; fig 2
In order to study a gamma-sarcoglycan-null mouse for a reduction in muscle pathology with collagen V1 deficiency, but no improvement in muscle function, Invitrogen perlecan antibody (Thermo Fisher Scientific, RT-794) was used in immunohistochemistry on mouse samples (fig 2). Hum Mol Genet (2016) ncbi
mouse monoclonal (A71)
  • immunocytochemistry; domestic rabbit; 1:150; fig 4
In order to determine the expression of corneal epithelial basement membrane components in different corneal stromal cell types, Invitrogen perlecan antibody (Thermo Scientific, CSI 001-71-02) was used in immunocytochemistry on domestic rabbit samples at 1:150 (fig 4). Mol Vis (2015) ncbi
mouse monoclonal (7B5)
  • western blot; human; 1:500; fig 6
In order to study the role of normal and tumor-associated fibroblasts in the pathogenesis of cervical cancer, Invitrogen perlecan antibody (Zymed, 13-4400) was used in western blot on human samples at 1:500 (fig 6). BMC Cancer (2015) ncbi
mouse monoclonal (7B5)
  • immunohistochemistry - frozen section; human
In order to characterize nerve hyperexcitability and neuromuscular junction remodeling in Schwartz-Jampel syndrome, Invitrogen perlecan antibody (Life Technologies, 7B5) was used in immunohistochemistry - frozen section on human samples . Neuromuscul Disord (2013) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry - frozen section; mouse; fig 3
In order to test if direct binding of anti-DNA antibody to the glomerular basement membrane important in experimental lupus nephritis, Invitrogen perlecan antibody (Thermo Fisher Scientific, A7L6) was used in immunohistochemistry - frozen section on mouse samples (fig 3). Kidney Int (2012) ncbi
mouse monoclonal (7B5)
In order to study heterogeneously glycosylated recombinant human perlecan domain 1, Invitrogen perlecan antibody (Invitrogen, 13-4400) was used . BMC Biotechnol (2010) ncbi
mouse monoclonal (7B5)
  • immunohistochemistry - frozen section; human; 1:10; fig 10
  • ELISA; human; 2 ug/ml; fig 1
In order to study peroxynitrite-induced damage within human atherosclerotic lesions, Invitrogen perlecan antibody (Zymed, 7B5) was used in immunohistochemistry - frozen section on human samples at 1:10 (fig 10) and in ELISA on human samples at 2 ug/ml (fig 1). Free Radic Biol Med (2010) ncbi
mouse monoclonal (7B5)
  • immunohistochemistry - paraffin section; human; 1:100; tbl 2
In order to compare the expression profiles of primary and metastatic brain tumors, Invitrogen perlecan antibody (Zymed, 13-4400) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 2). J Neurooncol (2010) ncbi
mouse monoclonal (7B5)
  • immunocytochemistry; human; 1:500; fig 5
In order to determine if pregnancy alters proteoglycan production in cervical fibroblasts, Invitrogen perlecan antibody (Zymed, 13-4400) was used in immunocytochemistry on human samples at 1:500 (fig 5). Acta Obstet Gynecol Scand (2008) ncbi
mouse monoclonal (7B5)
  • immunohistochemistry; human; fig 4
  • western blot; human; fig 6
In order to study the localization and function of perlecan in human fetal, postnatal, and mature hyaline cartilages, Invitrogen perlecan antibody (Zymed, 7B5) was used in immunohistochemistry on human samples (fig 4) and in western blot on human samples (fig 6). J Biol Chem (2006) ncbi
Santa Cruz Biotechnology
brown rat monoclonal (A7L6)
  • immunocytochemistry; human; 1:100; loading ...
Santa Cruz Biotechnology perlecan antibody (Santa, sc-33707) was used in immunocytochemistry on human samples at 1:100. elife (2019) ncbi
mouse monoclonal (E-6)
  • western blot; domestic rabbit; 1:1000; loading ...; fig 2b
Santa Cruz Biotechnology perlecan antibody (Santa Cruz Biotechnology, sc377219) was used in western blot on domestic rabbit samples at 1:1000 (fig 2b). Invest Ophthalmol Vis Sci (2018) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry; bovine; 1:100
Santa Cruz Biotechnology perlecan antibody (Santa Cruz Biotechnology, sc33707) was used in immunohistochemistry on bovine samples at 1:100. Osteoarthritis Cartilage (2015) ncbi
Abcam
mouse monoclonal
  • western blot; mouse; 1:500; fig 9a
Abcam perlecan antibody (Abcam, ab26265) was used in western blot on mouse samples at 1:500 (fig 9a). Development (2018) ncbi
mouse monoclonal (A74)
  • immunocytochemistry; human; 1:100; fig 4c
In order to develop a co-culture system to study endothelial cell and smooth muscle cell interactions, Abcam perlecan antibody (Abcam, ab23418) was used in immunocytochemistry on human samples at 1:100 (fig 4c). Tissue Eng Part C Methods (2017) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:200; fig s5
Abcam perlecan antibody (Abcam, 26265) was used in immunocytochemistry on human samples at 1:200 (fig s5). Sci Rep (2016) ncbi
mouse monoclonal
  • immunohistochemistry; human
Abcam perlecan antibody (abcam, ab26265) was used in immunohistochemistry on human samples . Biomaterials (2015) ncbi
EMD Millipore
brown rat monoclonal (A7L6)
  • immunohistochemistry; mouse; loading ...; fig 7e
EMD Millipore perlecan antibody (EMD Millipore, MAB1948P) was used in immunohistochemistry on mouse samples (fig 7e). J Clin Invest (2017) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry - frozen section; mouse; fig 7
EMD Millipore perlecan antibody (Millipore, MAB1948P) was used in immunohistochemistry - frozen section on mouse samples (fig 7). Skelet Muscle (2016) ncbi
brown rat monoclonal (A7L6)
  • western blot; human; 1:500; fig 3
EMD Millipore perlecan antibody (Millipore, MAB1948) was used in western blot on human samples at 1:500 (fig 3). PLoS ONE (2015) ncbi
brown rat monoclonal (A7L6)
  • western blot; brown rat; loading ...; fig 9d
EMD Millipore perlecan antibody (EMD Millipore, MAB1948P) was used in western blot on brown rat samples (fig 9d). J Cell Biol (2015) ncbi
brown rat monoclonal (A7L6)
  • immunohistochemistry - paraffin section; mouse; 1:50
EMD Millipore perlecan antibody (Millipore, MAB1948P) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. J Histochem Cytochem (2013) ncbi
Articles Reviewed
  1. Vögtle T, Sharma S, Mori J, Nagy Z, Semeniak D, Scandola C, et al. Heparan sulfates are critical regulators of the inhibitory megakaryocyte-platelet receptor G6b-B. elife. 2019;8: pubmed publisher
  2. Saikia P, Thangavadivel S, Medeiros C, Lassance L, de Oliveira R, Wilson S. IL-1 and TGF-β Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells. Invest Ophthalmol Vis Sci. 2018;59:5589-5598 pubmed publisher
  3. Amoasii L, Hildyard J, Li H, Sanchez Ortiz E, Mireault A, Caballero D, et al. Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy. Science. 2018;362:86-91 pubmed publisher
  4. Bird I, Kim S, Schweppe D, Caetano Lopes J, Robling A, Charles J, et al. The skeletal phenotype of achondrogenesis type 1A is caused exclusively by cartilage defects. Development. 2018;145: pubmed publisher
  5. Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  6. Caberlotto E, Ruiz L, Miller Z, Poletti M, Tadlock L. Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles. PLoS ONE. 2017;12:e0172624 pubmed publisher
  7. Chajra H, Auriol D, Joly F, Pagnon A, Rodrigues M, Allart S, et al. Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties. Clin Cosmet Investig Dermatol. 2016;9:461-472 pubmed
  8. Ganesan M, Finsterwalder R, Leb H, Resch U, Neumüller K, de Martin R, et al. Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells. Tissue Eng Part C Methods. 2017;23:38-49 pubmed publisher
  9. Fantauzzo K, Soriano P. PDGFR? regulates craniofacial development through homodimers and functional heterodimers with PDGFR?. Genes Dev. 2016;30:2443-2458 pubmed
  10. Foltz S, Luan J, Call J, Patel A, Peissig K, Fortunato M, et al. Four-week rapamycin treatment improves muscular dystrophy in a fukutin-deficient mouse model of dystroglycanopathy. Skelet Muscle. 2016;6:20 pubmed publisher
  11. de Greef J, Hamlyn R, Jensen B, O Campo Landa R, Levy J, Kobuke K, et al. Collagen VI deficiency reduces muscle pathology, but does not improve muscle function, in the γ-sarcoglycan-null mouse. Hum Mol Genet. 2016;25:1357-69 pubmed publisher
  12. Lee M, Goralczyk A, Kriszt R, Ang X, Badowski C, Li Y, et al. ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs. Sci Rep. 2016;6:21173 pubmed publisher
  13. Santhanam A, Torricelli A, Wu J, Marino G, Wilson S. Differential expression of epithelial basement membrane components nidogens and perlecan in corneal stromal cells in vitro. Mol Vis. 2015;21:1318-27 pubmed
  14. Reine T, Vuong T, Rutkovskiy A, Meen A, Vaage J, Jenssen T, et al. Serglycin in Quiescent and Proliferating Primary Endothelial Cells. PLoS ONE. 2015;10:e0145584 pubmed publisher
  15. Blocki A, Beyer S, Dewavrin J, Goralczyk A, Wang Y, Peh P, et al. Microcapsules engineered to support mesenchymal stem cell (MSC) survival and proliferation enable long-term retention of MSCs in infarcted myocardium. Biomaterials. 2015;53:12-24 pubmed publisher
  16. Fullár A, Dudás J, Oláh L, Hollósi P, Papp Z, Sobel G, et al. Remodeling of extracellular matrix by normal and tumor-associated fibroblasts promotes cervical cancer progression. BMC Cancer. 2015;15:256 pubmed publisher
  17. Colombelli C, Palmisano M, Eshed Eisenbach Y, Zambroni D, Pavoni E, Ferri C, et al. Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol. 2015;208:313-29 pubmed publisher
  18. Neu C, Novak T, Gilliland K, Marshall P, Calve S. Optical clearing in collagen- and proteoglycan-rich osteochondral tissues. Osteoarthritis Cartilage. 2015;23:405-13 pubmed publisher
  19. Bauché S, Boerio D, Davoine C, Bernard V, Stum M, Bureau C, et al. Peripheral nerve hyperexcitability with preterminal nerve and neuromuscular junction remodeling is a hallmark of Schwartz-Jampel syndrome. Neuromuscul Disord. 2013;23:998-1009 pubmed publisher
  20. Reine T, Grøndahl F, Jenssen T, Hadler Olsen E, Prydz K, Kolset S. Reduced sulfation of chondroitin sulfate but not heparan sulfate in kidneys of diabetic db/db mice. J Histochem Cytochem. 2013;61:606-16 pubmed publisher
  21. Krishnan M, Wang C, Marion T. Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice. Kidney Int. 2012;82:184-92 pubmed publisher
  22. Ellis A, Pan W, Yang G, Jones K, Chuang C, Whitelock J, et al. Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1. BMC Biotechnol. 2010;10:66 pubmed publisher
  23. Kennett E, Rees M, Malle E, Hammer A, Whitelock J, Davies M. Peroxynitrite modifies the structure and function of the extracellular matrix proteoglycan perlecan by reaction with both the protein core and the heparan sulfate chains. Free Radic Biol Med. 2010;49:282-93 pubmed publisher
  24. Liu Y, Carson Walter E, Cooper A, Winans B, Johnson M, Walter K. Vascular gene expression patterns are conserved in primary and metastatic brain tumors. J Neurooncol. 2010;99:13-24 pubmed publisher
  25. Akerud A, Dubicke A, Sennstrom M, Ekman Ordeberg G, Malmstrom A. Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery. Acta Obstet Gynecol Scand. 2008;87:1220-8 pubmed publisher
  26. Melrose J, Roughley P, Knox S, Smith S, Lord M, Whitelock J. The structure, location, and function of perlecan, a prominent pericellular proteoglycan of fetal, postnatal, and mature hyaline cartilages. J Biol Chem. 2006;281:36905-14 pubmed