This is a Validated Antibody Database (VAD) review about human optineurin, based on 13 published articles (read how Labome selects the articles), using optineurin antibody in all methods. It is aimed to help Labome visitors find the most suited optineurin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
optineurin synonym: ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; TFIIIA-INTP

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:5000; fig 5
  • western blot; human; 1:5000; fig 5
Abcam optineurin antibody (Abcam, ab23666) was used in western blot knockout validation on mouse samples at 1:5000 (fig 5) and in western blot on human samples at 1:5000 (fig 5). BMC Biol (2016) ncbi
Cayman Chemical
polyclonal
  • western blot knockout validation; mouse; 1:5000; fig 5
  • western blot knockout validation; human; 1:4000; fig 5
  • immunocytochemistry; human; 1:500; fig s5
Cayman Chemical optineurin antibody (Cayman Chemical, 100000) was used in western blot knockout validation on mouse samples at 1:5000 (fig 5), in western blot knockout validation on human samples at 1:4000 (fig 5) and in immunocytochemistry on human samples at 1:500 (fig s5). BMC Biol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-2)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 8e
Santa Cruz Biotechnology optineurin antibody (Santa Cruz, sc-166576) was used in immunohistochemistry on mouse samples at 1:200 (fig 8e). Cell Death Dis (2018) ncbi
mouse monoclonal (C-2)
  • western blot; human; loading ...; fig 6a
Santa Cruz Biotechnology optineurin antibody (Santa Cruz, sc-166576) was used in western blot on human samples (fig 6a). Cell Death Differ (2018) ncbi
mouse monoclonal (C-2)
  • western blot; human; 1:1000; fig s15
Santa Cruz Biotechnology optineurin antibody (Santa Cruz, sc-166576) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
mouse monoclonal (C-2)
  • western blot; mouse; 1:2000; fig 5
  • immunoprecipitation; human; fig s7
  • immunocytochemistry; human; 1:500; fig s5
  • western blot; human; 1:2000; fig 5
Santa Cruz Biotechnology optineurin antibody (Santa Cruz, sc-166576) was used in western blot on mouse samples at 1:2000 (fig 5), in immunoprecipitation on human samples (fig s7), in immunocytochemistry on human samples at 1:500 (fig s5) and in western blot on human samples at 1:2000 (fig 5). BMC Biol (2016) ncbi
mouse monoclonal (C-2)
  • western blot; mouse
Santa Cruz Biotechnology optineurin antibody (Santa Cruz Biotechnology, sc-166576) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
Proteintech Group
domestic rabbit polyclonal
  • immunohistochemistry; human; loading ...; fig 1s4e
Proteintech Group optineurin antibody (Proteintech, 10837-1-AP) was used in immunohistochemistry on human samples (fig 1s4e). elife (2019) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6d
Proteintech Group optineurin antibody (Proteintech, 10837-1-AP) was used in immunoprecipitation on human samples (fig 6d). Oncogene (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig s1a
In order to elucidate the mechanisms by which Rab-coupling protein contributes to metastasis, Proteintech Group optineurin antibody (Proteintech, 10837-1-AP) was used in western blot on human samples at 1:500 (fig s1a). Nat Commun (2017) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1d
Abcam optineurin antibody (Abcam, ab23666) was used in western blot on mouse samples at 1:1000 (fig 1d). J Biol Chem (2018) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:5000; fig 5
  • western blot; human; 1:5000; fig 5
Abcam optineurin antibody (Abcam, ab23666) was used in western blot knockout validation on mouse samples at 1:5000 (fig 5) and in western blot on human samples at 1:5000 (fig 5). BMC Biol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; tbl 4
In order to elucidate the function of the alpha2-linker of myosin VI, Abcam optineurin antibody (AbCam, ab-23666) was used in western blot on human samples at 1:1000 (tbl 4). Nat Struct Mol Biol (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 1h
In order to report that the interaction of the Atg12-Atg5-Atg16 complex and Atg8 with Atg19 is mutually exclusive, MilliporeSigma optineurin antibody (Sigma, HPA003279) was used in western blot on human samples at 1:500 (fig 1h). elife (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D2L8S)
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology optineurin antibody (Cell Signaling, 58981) was used in western blot on human samples (fig 3c). Front Cell Neurosci (2020) ncbi
domestic rabbit monoclonal (D2L8S)
  • western blot; human; loading ...; fig 2f
Cell Signaling Technology optineurin antibody (Cell Signaling, 58981) was used in western blot on human samples (fig 2f). Sci Adv (2019) ncbi
Cayman Chemical
polyclonal
  • western blot knockout validation; mouse; 1:5000; fig 5
  • western blot knockout validation; human; 1:4000; fig 5
  • immunocytochemistry; human; 1:500; fig s5
Cayman Chemical optineurin antibody (Cayman Chemical, 100000) was used in western blot knockout validation on mouse samples at 1:5000 (fig 5), in western blot knockout validation on human samples at 1:4000 (fig 5) and in immunocytochemistry on human samples at 1:500 (fig s5). BMC Biol (2016) ncbi
Articles Reviewed
  1. Huang Z, Zhao J, Wang W, Zhou J, Zhang J. Depletion of LncRNA NEAT1 Rescues Mitochondrial Dysfunction Through NEDD4L-Dependent PINK1 Degradation in Animal Models of Alzheimer's Disease. Front Cell Neurosci. 2020;14:28 pubmed publisher
  2. Yang M, Chen P, Liu J, Zhu S, Kroemer G, Klionsky D, et al. Clockophagy is a novel selective autophagy process favoring ferroptosis. Sci Adv. 2019;5:eaaw2238 pubmed publisher
  3. Zhang P, Fan B, Yang P, Temirov J, Messing J, Kim H, et al. Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. elife. 2019;8: pubmed publisher
  4. Wang D, Xu Q, Yuan Q, Jia M, Niu H, Liu X, et al. Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene. 2019;38:3458-3474 pubmed publisher
  5. Chen K, Dai H, Yuan J, Chen J, Lin L, Zhang W, et al. Optineurin-mediated mitophagy protects renal tubular epithelial cells against accelerated senescence in diabetic nephropathy. Cell Death Dis. 2018;9:105 pubmed publisher
  6. Goiran T, Duplan E, Rouland L, El Manaa W, Lauritzen I, Dunys J, et al. Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation. Cell Death Differ. 2018;25:873-884 pubmed publisher
  7. Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
  8. Gundry C, Marco S, Rainero E, Miller B, Dornier E, Mitchell L, et al. Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion. Nat Commun. 2017;8:14646 pubmed publisher
  9. Fracchiolla D, Sawa Makarska J, Zens B, Ruiter A, Zaffagnini G, Brezovich A, et al. Mechanism of cargo-directed Atg8 conjugation during selective autophagy. elife. 2016;5: pubmed publisher
  10. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  11. Pourcelot M, Zemirli N, Silva da Costa L, Loyant R, Garcin D, Vitour D, et al. The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing. BMC Biol. 2016;14:69 pubmed publisher
  12. Wollscheid H, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, et al. Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain. Nat Struct Mol Biol. 2016;23:300-308 pubmed publisher
  13. Sharma A, Huard C, Vernochet C, Ziemek D, Knowlton K, Tyminski E, et al. Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6. PLoS ONE. 2014;9:e92608 pubmed publisher