This is a Validated Antibody Database (VAD) review about human nucleolin, based on 43 published articles (read how Labome selects the articles), using nucleolin antibody in all methods. It is aimed to help Labome visitors find the most suited nucleolin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
nucleolin synonym: C23; Nsr1

Santa Cruz Biotechnology
mouse monoclonal (MS-3)
  • immunocytochemistry; human; 1:100; loading ...; fig 2h
Santa Cruz Biotechnology nucleolin antibody (SantaCruz, sc-8031) was used in immunocytochemistry on human samples at 1:100 (fig 2h). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (MS-3)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology nucleolin antibody (SantaCruz, sc-8031) was used in western blot on human samples (fig 3a). Open Biol (2016) ncbi
mouse monoclonal (D-6)
  • western blot; human; 1:1000; loading ...; fig 3a
In order to investigate the DNA/nucleosome and histone chaperone binding activities of H1.X, Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, SC-17826) was used in western blot on human samples at 1:1000 (fig 3a). Mol Cell Biol (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; loading ...; fig 1a
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, SC-8031) was used in western blot on human samples (fig 1a). Oncotarget (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 4
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (D-6)
  • immunocytochemistry; human; 200 ng/ml; fig s2a
In order to test if NOL1activates transcription of cyclin D1, Santa Cruz Biotechnology nucleolin antibody (santa cruz, sc-17826) was used in immunocytochemistry on human samples at 200 ng/ml (fig s2a). J Cell Sci (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 3
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 3). IUBMB Life (2016) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig S5
Santa Cruz Biotechnology nucleolin antibody (Santacruz, sc-8031) was used in immunoprecipitation on human samples (fig S5). Oncotarget (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 2
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 2). Oncogene (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in western blot on human samples . Neurobiol Aging (2015) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-515312) was used in western blot on human samples (fig 2a). Toxicol Lett (2015) ncbi
mouse monoclonal (MS-3)
  • western blot; human; 1:1000
In order to study the role of Tn expression and post-translationally modifications in pancreatic ductal adenocarcinoma, Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in western blot on human samples at 1:1000. Mol Cancer (2015) ncbi
mouse monoclonal (MS-3)
  • western blot; human; 1:1000; fig 3
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc8031) was used in western blot on human samples at 1:1000 (fig 3). J Mol Biol (2015) ncbi
mouse monoclonal (H-6)
  • immunocytochemistry; mouse; 1:200; fig s1
In order to report that Tat-activating regulatory DNA-binding protein-43 regulates the synthesis of the interleukin-6 and -10 splicing activating compartment, Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-55486) was used in immunocytochemistry on mouse samples at 1:200 (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (H-6)
  • western blot; rat; 1:500
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-55486) was used in western blot on rat samples at 1:500. Cell Death Dis (2014) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
In order to characterize a potential treatment for glioblastoma by interfering with Erb1, Ras and nucleolin interactions, Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig 1). Oncotarget (2014) ncbi
mouse monoclonal (D-6)
  • western blot; human; 1:1000; fig 4
In order to report that Koelreuteria formosana ethanolic extract inhibits the invasion and migration of renal cell carcinoma cells, Santa Cruz Biotechnology nucleolin antibody (Santa Cruz Biotechnology, sc-17826) was used in western blot on human samples at 1:1000 (fig 4). Mol Med Rep (2014) ncbi
mouse monoclonal (MS-3)
  • immunocytochemistry; mouse
Santa Cruz Biotechnology nucleolin antibody (Santa Cruz, sc-8031) was used in immunocytochemistry on mouse samples . Stem Cells Dev (2014) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig s1c
Santa Cruz Biotechnology nucleolin antibody (Santa, sc-8031) was used in immunoprecipitation on human samples (fig s1c). Nucleic Acids Res (2014) ncbi
Abcam
domestic rabbit monoclonal
  • western blot; mouse; loading ...; fig 7i
Abcam nucleolin antibody (Abcam, ab129200) was used in western blot on mouse samples (fig 7i). J Cell Biol (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:300; loading ...; fig 3c
Abcam nucleolin antibody (Abcam, ab22758) was used in immunocytochemistry on human samples at 1:300 (fig 3c). PLoS Genet (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7d
Abcam nucleolin antibody (Abcam, ab22758) was used in western blot on mouse samples at 1:1000 (fig 7d). J Biol Chem (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4b
Abcam nucleolin antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig 4a) and in western blot on human samples (fig 4b). J Am Chem Soc (2019) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6a
Abcam nucleolin antibody (Abcam, ab22758) was used in immunoprecipitation on human samples (fig 6a). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1f
Abcam nucleolin antibody (Abcam, ab70493) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 1f). Dis Model Mech (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
In order to report the interactome of all dipeptide repeat proteins, Abcam nucleolin antibody (Abcam, ab22758) was used in western blot on human samples (fig 1c). Cell (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 1
In order to study how PARylation regulates Top1 nuclear dynamics, Abcam nucleolin antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig 1). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 6a
  • western blot; human; 1:100; fig 7a
In order to examine the composition of mitochondrial proteins in cases of fatal familial insomnia, Abcam nucleolin antibody (Abcam, Ab22758) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 6a) and in western blot on human samples at 1:100 (fig 7a). Brain Pathol (2017) ncbi
Invitrogen
mouse monoclonal (ZN004)
  • western blot; human; fig 4b
In order to propose that WRN interacts with HDACs to facilitate activity of stalled replication forks under conditions of replication stress, Invitrogen nucleolin antibody (Life Technologies, 396400) was used in western blot on human samples (fig 4b). J Biol Chem (2016) ncbi
mouse monoclonal (ZN004)
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 2
In order to characterize viral replication compartments isolated from adenovirus-infected cells for biochemical, morphological, and functional properties, Invitrogen nucleolin antibody (Invitrogen, ZN004) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 2). J Virol (2016) ncbi
mouse monoclonal (4E2)
  • immunocytochemistry; human; 1:1000; loading ...; fig 3g
In order to propose that DGCR8 helps to recruit the exosome complex to RNAs and induce their degradation, Invitrogen nucleolin antibody (Thermo Scientific, MA1-20800) was used in immunocytochemistry on human samples at 1:1000 (fig 3g). Mol Cell (2015) ncbi
mouse monoclonal (ZN004)
  • immunocytochemistry; human; 1:100
In order to study allorecognition using Botryllus schlosseri, Invitrogen nucleolin antibody (Life Technologies, 39-6400) was used in immunocytochemistry on human samples at 1:100. Immunogenetics (2015) ncbi
mouse monoclonal (ZN004)
  • immunocytochemistry; human; 1:100; fig 2
In order to examine the localization of nucleophosmin in thyroid tumors, Invitrogen nucleolin antibody (Zymed Laboratories, Clone ZN004) was used in immunocytochemistry on human samples at 1:100 (fig 2). Endocr Pathol (2011) ncbi
mouse monoclonal (ZN004)
  • immunocytochemistry; human; 1:100
In order to describe a case of a therapy-responder acute myeloid leukemia patient bearing two novel NPM1 mutations, Invitrogen nucleolin antibody (Zymed, ZN004) was used in immunocytochemistry on human samples at 1:100. Hematol Oncol (2010) ncbi
mouse monoclonal (ZN004)
  • immunocytochemistry; human
  • western blot; human
In order to identify APE1 partners, Invitrogen nucleolin antibody (Zymed Laboratories, Invitrogen, ZN004) was used in immunocytochemistry on human samples and in western blot on human samples . Mol Cell Biol (2009) ncbi
Enzo Life Sciences
mouse monoclonal (4E2)
  • immunocytochemistry; human
Enzo Life Sciences nucleolin antibody (Enzo Life Sciences, KAM-CP100) was used in immunocytochemistry on human samples . J Virol (2015) ncbi
mouse monoclonal (4E2)
  • western blot; human
Enzo Life Sciences nucleolin antibody (Stressgen, KAM-CP100) was used in western blot on human samples . FEBS Lett (2013) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • western blot; human; 1:4000; fig 4c
Novus Biologicals nucleolin antibody (Novus, NB600-241) was used in western blot on human samples at 1:4000 (fig 4c). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4C7O)
  • immunoprecipitation; mouse; loading ...; fig 4f
Cell Signaling Technology nucleolin antibody (Cell Signaling, 14574) was used in immunoprecipitation on mouse samples (fig 4f). Sci Rep (2020) ncbi
domestic rabbit monoclonal (D4C7O)
  • immunocytochemistry; human; loading ...; fig 1c
Cell Signaling Technology nucleolin antibody (Cell Signaling Technology, 14574) was used in immunocytochemistry on human samples (fig 1c). Cell Death Discov (2020) ncbi
domestic rabbit monoclonal (D4C7O)
  • western blot; mouse; loading ...; fig 5c
Cell Signaling Technology nucleolin antibody (Cell Signaling, 14574S) was used in western blot on mouse samples (fig 5c). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (D4C7O)
  • immunocytochemistry; human; 1:500; loading ...; fig 2b
In order to study the anti-cancer activity and cytotoxicity of acrolein, Cell Signaling Technology nucleolin antibody (Cell Signaling, 14574) was used in immunocytochemistry on human samples at 1:500 (fig 2b). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D4C7O)
  • immunocytochemistry; human; 1:100; fig 4
In order to study key functions in nucleolar localisation and polyphosphoinositide interaction by a polybasic motif in ErbB3-binding-protein 1 (EBP1), Cell Signaling Technology nucleolin antibody (Cell Signaling, 14574) was used in immunocytochemistry on human samples at 1:100 (fig 4). Biochem J (2016) ncbi
Articles Reviewed
  1. Song L, Tian X, Schekman R. Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1. J Cell Biol. 2021;220: pubmed publisher
  2. Park K, Ryoo J, Jeong H, Kim M, Lee S, Hwang S, et al. Aicardi-Goutières syndrome-associated gene SAMHD1 preserves genome integrity by preventing R-loop formation at transcription-replication conflict regions. PLoS Genet. 2021;17:e1009523 pubmed publisher
  3. Soliman S, Stark A, Gardner M, Harshman S, Breece C, Amari F, et al. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. Sci Rep. 2020;10:7138 pubmed publisher
  4. Saini N, Sarin A. Nucleolar localization of the Notch4 intracellular domain underpins its regulation of the cellular response to genotoxic stressors. Cell Death Discov. 2020;6:7 pubmed publisher
  5. Brody M, Vanhoutte D, Bakshi C, Liu R, Correll R, Sargent M, et al. Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis. J Biol Chem. 2019;294:8918-8929 pubmed publisher
  6. Wang F, Zhou S, Qi D, Xiang S, Wong E, Wang X, et al. Nucleolin Is a Functional Binding Protein for Salinomycin in Neuroblastoma Stem Cells. J Am Chem Soc. 2019;141:3613-3622 pubmed publisher
  7. Castanotto D, Zhang X, Alluin J, Zhang X, Rüger J, Armstrong B, et al. A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus. Proc Natl Acad Sci U S A. 2018;115:E5756-E5765 pubmed publisher
  8. Li S, Mi L, Yu L, Yu Q, Liu T, Wang G, et al. Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis. Proc Natl Acad Sci U S A. 2017;114:E7111-E7120 pubmed publisher
  9. Evsyukov V, Domanskyi A, Bierhoff H, Gispert S, Mustafa R, Schlaudraff F, et al. Genetic mutations linked to Parkinson's disease differentially control nucleolar activity in pre-symptomatic mouse models. Dis Model Mech. 2017;10:633-643 pubmed publisher
  10. Coccia M, Rossi A, Riccio A, Trotta E, Santoro M. Human NF-κB repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance. Proc Natl Acad Sci U S A. 2017;114:1045-1050 pubmed publisher
  11. Samson A, Ho B, Au A, Schoenwaelder S, Smyth M, Bottomley S, et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol. 2016;6: pubmed
  12. Lee K, Zhang P, Kim H, Mitrea D, Sarkar M, Freibaum B, et al. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell. 2016;167:774-788.e17 pubmed publisher
  13. Wang H, Chen T, Weng C, Yang C, Tang M. Acrolein preferentially damages nucleolus eliciting ribosomal stress and apoptosis in human cancer cells. Oncotarget. 2016;7:80450-80464 pubmed publisher
  14. Kehrli K, Phelps M, Lazarchuk P, Chen E, Monnat R, Sidorova J. Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest. J Biol Chem. 2016;291:24487-24503 pubmed
  15. Okuwaki M, Abe M, Hisaoka M, Nagata K. Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X. Mol Cell Biol. 2016;36:2681-2696 pubmed publisher
  16. Das S, Rehman I, Ghosh A, Sengupta S, Majumdar P, Jana B, et al. Poly(ADP-ribose) polymers regulate DNA topoisomerase I (Top1) nuclear dynamics and camptothecin sensitivity in living cells. Nucleic Acids Res. 2016;44:8363-75 pubmed publisher
  17. Robijns J, Molenberghs F, Sieprath T, Corne T, Verschuuren M, De Vos W. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016;6:30325 pubmed publisher
  18. Kim Y, Lee M, Kim H, Kim J. Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. Oncotarget. 2016;7:80350-80362 pubmed publisher
  19. Terrier O, Carron C, de Chassey B, Dubois J, Traversier A, Julien T, et al. Nucleolin interacts with influenza A nucleoprotein and contributes to viral ribonucleoprotein complexes nuclear trafficking and efficient influenza viral replication. Sci Rep. 2016;6:29006 pubmed publisher
  20. Frau Méndez M, Fernández Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Río J, et al. Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. Brain Pathol. 2017;27:95-106 pubmed publisher
  21. Karlsson T, Altankhuyag A, Dobrovolska O, Turcu D, Lewis A. A polybasic motif in ErbB3-binding protein 1 (EBP1) has key functions in nucleolar localization and polyphosphoinositide interaction. Biochem J. 2016;473:2033-47 pubmed publisher
  22. Hong J, Lee J, Chung I. Telomerase activates transcription of cyclin D1 gene through an interaction with NOL1. J Cell Sci. 2016;129:1566-79 pubmed publisher
  23. Bober J, Olsnes S, Kostas M, Bogacz M, Zakrzewska M, Otlewski J. Identification of new FGF1 binding partners-Implications for its intracellular function. IUBMB Life. 2016;68:242-51 pubmed publisher
  24. Hidalgo P, Anzures L, Hernández Mendoza A, Guerrero A, Wood C, Valdés M, et al. Morphological, Biochemical, and Functional Study of Viral Replication Compartments Isolated from Adenovirus-Infected Cells. J Virol. 2016;90:3411-27 pubmed publisher
  25. Macias S, Cordiner R, Gautier P, Plass M, Cáceres J. DGCR8 Acts as an Adaptor for the Exosome Complex to Degrade Double-Stranded Structured RNAs. Mol Cell. 2015;60:873-85 pubmed publisher
  26. Nogales V, Reinhold W, Varma S, Martinez Cardus A, Moutinho C, Moran S, et al. Epigenetic inactivation of the putative DNA/RNA helicase SLFN11 in human cancer confers resistance to platinum drugs. Oncotarget. 2016;7:3084-97 pubmed publisher
  27. Felli N, Errico M, Pedini F, Petrini M, Puglisi R, Bellenghi M, et al. AP2α controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene. 2016;35:3016-26 pubmed publisher
  28. Taketa D, Nydam M, Langenbacher A, Rodriguez D, Sanders E, De Tomaso A. Molecular evolution and in vitro characterization of Botryllus histocompatibility factor. Immunogenetics. 2015;67:605-23 pubmed publisher
  29. Su W, Hsu S, Lee Y, Jeng K, Lai M. A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription. J Virol. 2015;89:11245-55 pubmed publisher
  30. Li M, Pehar M, Liu Y, Bhattacharyya A, Zhang S, O Riordan K, et al. The amyloid precursor protein (APP) intracellular domain regulates translation of p44, a short isoform of p53, through an IRES-dependent mechanism. Neurobiol Aging. 2015;36:2725-36 pubmed publisher
  31. Chiou Y, Liou S, Wong R, Chen C, Lee H. Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR-mutated lung cancer via nickel-induced microRNA-21 expression. Toxicol Lett. 2015;237:46-54 pubmed publisher
  32. Hofmann B, Schlüter L, Lange P, Mercanoglu B, Ewald F, Fölster A, et al. COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. Mol Cancer. 2015;14:109 pubmed publisher
  33. Szerlong H, Herman J, Krause C, DeLuca J, Skoultchi A, Winger Q, et al. Proteomic characterization of the nucleolar linker histone H1 interaction network. J Mol Biol. 2015;427:2056-71 pubmed publisher
  34. Lee S, Lee T, Lee E, Kang S, Park A, Kim S, et al. Identification of a subnuclear body involved in sequence-specific cytokine RNA processing. Nat Commun. 2015;6:5791 pubmed publisher
  35. Aries A, Whitcomb J, Shao W, Komati H, Saleh M, Nemer M. Caspase-1 cleavage of transcription factor GATA4 and regulation of cardiac cell fate. Cell Death Dis. 2014;5:e1566 pubmed publisher
  36. Goldshmit Y, Trangle S, Kloog Y, Pinkas Kramarski R. Interfering with the interaction between ErbB1, nucleolin and Ras as a potential treatment for glioblastoma. Oncotarget. 2014;5:8602-13 pubmed
  37. Lin C, Chen P, Hsu L, Kuo D, Chu S, Hsieh Y. Inhibition of the invasion and migration of renal carcinoma 786‑o‑si3 cells in vitro and in vivo by Koelreuteria formosana extract. Mol Med Rep. 2014;10:3334-42 pubmed publisher
  38. Lührig S, Siamishi I, Tesmer Wolf M, Zechner U, Engel W, Nolte J. Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells. Stem Cells Dev. 2014;23:2862-74 pubmed publisher
  39. Ullius A, Lüscher Firzlaff J, Costa I, Walsemann G, Forst A, Gusmao E, et al. The interaction of MYC with the trithorax protein ASH2L promotes gene transcription by regulating H3K27 modification. Nucleic Acids Res. 2014;42:6901-20 pubmed publisher
  40. Das S, Cong R, Shandilya J, Senapati P, Moindrot B, Monier K, et al. Characterization of nucleolin K88 acetylation defines a new pool of nucleolin colocalizing with pre-mRNA splicing factors. FEBS Lett. 2013;587:417-24 pubmed publisher
  41. Pianta A, Puppin C, Passon N, Franzoni A, Romanello M, Tell G, et al. Nucleophosmin delocalization in thyroid tumour cells. Endocr Pathol. 2011;22:18-23 pubmed publisher
  42. Pianta A, Fabbro D, Damiani D, Tiribelli M, Fanin R, Franzoni A, et al. Two novel NPM1 mutations in a therapy-responder AML patient. Hematol Oncol. 2010;28:151-5 pubmed publisher
  43. Vascotto C, Fantini D, Romanello M, Cesaratto L, Deganuto M, Leonardi A, et al. APE1/Ref-1 interacts with NPM1 within nucleoli and plays a role in the rRNA quality control process. Mol Cell Biol. 2009;29:1834-54 pubmed publisher