This is a Validated Antibody Database (VAD) review about human nephrin, based on 21 published articles (read how Labome selects the articles), using nephrin antibody in all methods. It is aimed to help Labome visitors find the most suited nephrin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
nephrin synonym: CNF; NPHN; nephrin

Knockout validation
Progen
guinea-pigs polyclonal
  • western blot knockout validation; mouse; fig s1
  • immunohistochemistry; mouse; fig s1
In order to determine the genetic factors that regulate slit diaphragm architecture, Progen nephrin antibody (Progen, gp-NP2) was used in western blot knockout validation on mouse samples (fig s1) and in immunohistochemistry on mouse samples (fig s1). JCI Insight (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR20993)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3b
Abcam nephrin antibody (Abcam, ab216341) was used in immunohistochemistry on mouse samples at 1:200 (fig 3b). Int J Mol Med (2021) ncbi
domestic rabbit monoclonal (EPR20993)
  • western blot; mouse; 1:1000; loading ...; fig 1f
Abcam nephrin antibody (Abcam, ab216341) was used in western blot on mouse samples at 1:1000 (fig 1f). BMC Nephrol (2021) ncbi
domestic rabbit monoclonal (EPR20993)
  • western blot; rat; 1:1000; loading ...; fig 1e
Abcam nephrin antibody (Abcam, ab216341) was used in western blot on rat samples at 1:1000 (fig 1e). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (EPR20993)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3a, 3b
  • western blot; mouse; loading ...; fig 3c, 5h
Abcam nephrin antibody (Abcam, ab216341) was used in immunohistochemistry - paraffin section on mouse samples (fig 3a, 3b) and in western blot on mouse samples (fig 3c, 5h). J Cell Mol Med (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; fig 3
Abcam nephrin antibody (Abcam, ab183099) was used in immunohistochemistry - paraffin section on rat samples (fig 3). Evid Based Complement Alternat Med (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-12)
  • immunocytochemistry; mouse; loading ...; fig 2b
  • immunohistochemistry; rat; 1:50; loading ...; fig 3
Santa Cruz Biotechnology nephrin antibody (Santa Cruz Biotech, sc-377246) was used in immunocytochemistry on mouse samples (fig 2b) and in immunohistochemistry on rat samples at 1:50 (fig 3). Evid Based Complement Alternat Med (2020) ncbi
mouse monoclonal (B-12)
  • western blot; mouse; 1:500; fig 1D
Santa Cruz Biotechnology nephrin antibody (Santa Cruz, sc-377246) was used in western blot on mouse samples at 1:500 (fig 1D). PLoS ONE (2016) ncbi
mouse monoclonal (B-12)
  • western blot; rat
Santa Cruz Biotechnology nephrin antibody (Santa Cruz Biotechnology, sc-377246) was used in western blot on rat samples . Redox Biol (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3f
Invitrogen nephrin antibody (Thermo Fisher Scientific, PA5-91907) was used in western blot on mouse samples (fig 3f). JCI Insight (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig 3a
Invitrogen nephrin antibody (Thermo Fisher Scientific, PA5?\20330) was used in western blot on mouse samples at 1:2000 (fig 3a). EMBO Mol Med (2019) ncbi
Enzo Life Sciences
domestic rabbit polyclonal (Y17-R)
  • immunocytochemistry; human; loading ...; fig 4b
In order to investigate whether kidney podocytes exhibit stiffness sensitivity, Enzo Life Sciences nephrin antibody (Enzo, 810-016-R100) was used in immunocytochemistry on human samples (fig 4b). Sci Rep (2017) ncbi
R&D Systems
domestic goat polyclonal
  • immunocytochemistry; mouse; 1:50; loading ...; fig 1a
R&D Systems nephrin antibody (R&D, AF3159) was used in immunocytochemistry on mouse samples at 1:50 (fig 1a). Nat Commun (2021) ncbi
Progen
guinea-pigs polyclonal
  • immunocytochemistry; mouse; loading ...; fig 1b
Progen nephrin antibody (PROGEN, GP-N2) was used in immunocytochemistry on mouse samples (fig 1b). Cells (2021) ncbi
guinea-pigs polyclonal
  • immunocytochemistry; human; loading ...; fig 1b
  • immunohistochemistry; human; loading ...; fig 1e
Progen nephrin antibody (Progen, GP-N2) was used in immunocytochemistry on human samples (fig 1b) and in immunohistochemistry on human samples (fig 1e). Cell Stem Cell (2019) ncbi
guinea-pigs polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2f
  • immunocytochemistry; mouse; loading ...; fig 2f
Progen nephrin antibody (Progene, Gp-N2) was used in immunohistochemistry - frozen section on mouse samples (fig 2f) and in immunocytochemistry on mouse samples (fig 2f). Dev Cell (2018) ncbi
guinea-pigs polyclonal
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 1f
Progen nephrin antibody (Progen, GP-N2) was used in immunohistochemistry - frozen section on mouse samples at 1:300 (fig 1f). PLoS ONE (2018) ncbi
guinea-pigs polyclonal
  • immunohistochemistry; human; loading ...; fig 1b
Progen nephrin antibody (Progen, GP-N2) was used in immunohistochemistry on human samples (fig 1b). J Clin Invest (2018) ncbi
guinea-pigs polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1g
In order to determine the function of thymosin in the kidneys, Progen nephrin antibody (Progen, GP-N2) was used in immunohistochemistry - paraffin section on mouse samples (fig 1g). Kidney Int (2016) ncbi
guinea-pigs polyclonal
  • western blot knockout validation; mouse; fig s1
  • immunohistochemistry; mouse; fig s1
In order to determine the genetic factors that regulate slit diaphragm architecture, Progen nephrin antibody (Progen, gp-NP2) was used in western blot knockout validation on mouse samples (fig s1) and in immunohistochemistry on mouse samples (fig s1). JCI Insight (2016) ncbi
guinea-pigs polyclonal
  • immunocytochemistry; mouse; 1:100; tbl 1
In order to report a protocol to obtain and utilize a three-dimensional podocyte-endothelial co-culture to measure albumin permeability, Progen nephrin antibody (Progen, GP-N2) was used in immunocytochemistry on mouse samples at 1:100 (tbl 1). Eur J Pharm Sci (2016) ncbi
guinea-pigs polyclonal
  • immunohistochemistry - paraffin section; human; fig 3
In order to generate and characterize podocytes derived from iPS cells, Progen nephrin antibody (Progen Biotechnik, GP-N2) was used in immunohistochemistry - paraffin section on human samples (fig 3). J Am Soc Nephrol (2016) ncbi
Articles Reviewed
  1. Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  2. Ren J, Xu Y, Lu X, Wang L, Ide S, Hall G, et al. Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration. JCI Insight. 2021;6: pubmed publisher
  3. Zhao J, Chen J, Li Y, Xia L, Wu Y. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48: pubmed publisher
  4. Maier J, Rogg M, Helmstädter M, Sammarco A, Walz G, Werner M, et al. A Novel Model for Nephrotic Syndrome Reveals Associated Dysbiosis of the Gut Microbiome and Extramedullary Hematopoiesis. Cells. 2021;10: pubmed publisher
  5. Xu X, Huang X, Zhang L, Huang X, Qin Z, Hua F. Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway. BMC Nephrol. 2021;22:218 pubmed publisher
  6. Sha S, Shen X, Cao Y, Qu L. Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer's disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway. Aging (Albany NY). 2021;13:15285-15306 pubmed publisher
  7. Wang T, Gao Y, Yue R, Wang X, Shi Y, Xu J, et al. Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTOR/NF-κB/NLRP3 Axis. Evid Based Complement Alternat Med. 2020;2020:2735714 pubmed publisher
  8. Low J, Li P, Chew E, Zhou B, Suzuki K, Zhang T, et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell. 2019;25:373-387.e9 pubmed publisher
  9. Huang X, Xue H, Ma J, Zhang Y, Zhang J, Liu Y, et al. Salidroside ameliorates Adriamycin nephropathy in mice by inhibiting β-catenin activity. J Cell Mol Med. 2019;23:4443-4453 pubmed publisher
  10. Lin C, Hsu Y, Huang Y, Shih Y, Wang C, Chiang W, et al. A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction. EMBO Mol Med. 2019;11: pubmed publisher
  11. Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  12. Yasuda Yamahara M, Rogg M, Yamahara K, Maier J, Huber T, Schell C. AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes. PLoS ONE. 2018;13:e0200487 pubmed publisher
  13. Maeda K, Otomo K, Yoshida N, Abu Asab M, Ichinose K, Nishino T, et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J Clin Invest. 2018;128:3445-3459 pubmed publisher
  14. Hu M, Azeloglu E, Ron A, Tran Ba K, Calizo R, Tavassoly I, et al. A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep. 2017;7:43934 pubmed publisher
  15. Vasilopoulou E, Kolatsi Joannou M, Lindenmeyer M, White K, Robson M, Cohen C, et al. Loss of endogenous thymosin β4 accelerates glomerular disease. Kidney Int. 2016;90:1056-1070 pubmed publisher
  16. Grahammer F, Wigge C, Schell C, Kretz O, Patrakka J, Schneider S, et al. A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes. JCI Insight. 2016;1: pubmed publisher
  17. Wei M, He W, Lu X, Ni L, Yang Y, Chen L, et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-?/Smad Signaling. Evid Based Complement Alternat Med. 2016;2016:5031890 pubmed publisher
  18. Zea A, Stewart T, Ascani J, Tate D, Finkel Jimenez B, Wilk A, et al. Activation of the IL-2 Receptor in Podocytes: A Potential Mechanism for Podocyte Injury in Idiopathic Nephrotic Syndrome?. PLoS ONE. 2016;11:e0157907 pubmed publisher
  19. Li M, Corbelli A, Watanabe S, Armelloni S, Ikehata M, Parazzi V, et al. Three-dimensional podocyte-endothelial cell co-cultures: Assembly, validation, and application to drug testing and intercellular signaling studies. Eur J Pharm Sci. 2016;86:1-12 pubmed publisher
  20. Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, Sakuma T, et al. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation. J Am Soc Nephrol. 2016;27:1778-91 pubmed publisher
  21. Ndisang J, Tiwari S. Mechanisms by which heme oxygenase rescue renal dysfunction in obesity. Redox Biol. 2014;2:1029-37 pubmed publisher