This is a Validated Antibody Database (VAD) review about human mesothelin, based on 10 published articles (read how Labome selects the articles), using mesothelin antibody in all methods. It is aimed to help Labome visitors find the most suited mesothelin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
mesothelin synonym: MPF; SMRP

Knockout validation
Invitrogen
domestic rabbit monoclonal (SP74)
  • immunohistochemistry knockout validation; human; 1:50; loading ...; fig 6a, 6b
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1b, 5b, 5c, 7a, 7b
Invitrogen mesothelin antibody (Thermo Fisher, SP74) was used in immunohistochemistry knockout validation on human samples at 1:50 (fig 6a, 6b) and in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1b, 5b, 5c, 7a, 7b). Int J Mol Sci (2022) ncbi
Invitrogen
domestic rabbit monoclonal (SP74)
  • immunocytochemistry knockout validation; human; 1:50; loading ...; fig 2e
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1e
Invitrogen mesothelin antibody (Thermo Fisher, MA5-16378) was used in immunocytochemistry knockout validation on human samples at 1:50 (fig 2e) and in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1e). Oncogenesis (2020) ncbi
Invitrogen
domestic rabbit monoclonal (SP74)
  • immunohistochemistry knockout validation; human; 1:50; loading ...; fig 6a, 6b
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1b, 5b, 5c, 7a, 7b
Invitrogen mesothelin antibody (Thermo Fisher, SP74) was used in immunohistochemistry knockout validation on human samples at 1:50 (fig 6a, 6b) and in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1b, 5b, 5c, 7a, 7b). Int J Mol Sci (2022) ncbi
domestic rabbit monoclonal (SP74)
  • immunocytochemistry knockout validation; human; 1:50; loading ...; fig 2e
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1e
Invitrogen mesothelin antibody (Thermo Fisher, MA5-16378) was used in immunocytochemistry knockout validation on human samples at 1:50 (fig 2e) and in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1e). Oncogenesis (2020) ncbi
mouse monoclonal (5B2)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3f
Invitrogen mesothelin antibody (Thermo Fisher, MS-1320-S0) was used in immunohistochemistry - paraffin section on human samples (fig 3f). JCI Insight (2018) ncbi
mouse monoclonal (5B2)
  • immunohistochemistry - paraffin section; human; 1:10; fig 2
In order to characterize the diagnostic value of mesothelin and IMP3 in differentiatiating pancreatic ductal adenocarcinoma from chronic pancreatitis, Invitrogen mesothelin antibody (Labvision, MA5-11918) was used in immunohistochemistry - paraffin section on human samples at 1:10 (fig 2). Pathol Res Pract (2016) ncbi
mouse monoclonal (5B2)
  • immunohistochemistry; human; 1:30; loading ...; fig 4b
In order to develop a new type of T-cell chimeric antigen receptor (T-CAR) immunotherapy for solid tumors., Invitrogen mesothelin antibody (Thermo Scientific, MS-1320) was used in immunohistochemistry on human samples at 1:30 (fig 4b). Cancer Immunol Res (2015) ncbi
Abcam
domestic rabbit monoclonal
  • immunohistochemistry; human; 1:200; loading ...; fig 2a
Abcam mesothelin antibody (Abcam, ab133489) was used in immunohistochemistry on human samples at 1:200 (fig 2a). Front Oncol (2022) ncbi
domestic rabbit monoclonal (SP74)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1
In order to investigate the efficacy of codelivering engineered T cells and STING agonists in cancer therapy, Abcam mesothelin antibody (Abcam, ab93620) was used in immunohistochemistry - paraffin section on human samples (fig 1). J Clin Invest (2017) ncbi
Rockland Immunochemicals
mouse monoclonal (MN-1)
  • western blot; human; fig 1c
Rockland Immunochemicals mesothelin antibody (Rockland Immunochemicals, 200-301-A88) was used in western blot on human samples (fig 1c). Mol Cancer Ther (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-1)
  • western blot; human; 1:250; loading ...; fig 3a
Santa Cruz Biotechnology mesothelin antibody (Santa Cruz, sc-271540) was used in western blot on human samples at 1:250 (fig 3a). PLoS ONE (2017) ncbi
Leica Biosystems
mouse monoclonal (5B2)
  • immunohistochemistry - paraffin section; human; 1:30
Leica Biosystems mesothelin antibody (Novocastra, 5B2) was used in immunohistochemistry - paraffin section on human samples at 1:30. Hum Pathol (2014) ncbi
Articles Reviewed
  1. Li Y, Tian W, Zhang H, Zhang Z, Zhao Q, Chang L, et al. MSLN Correlates With Immune Infiltration and Chemoresistance as a Prognostic Biomarker in Ovarian Cancer. Front Oncol. 2022;12:830570 pubmed publisher
  2. Nunes M, Pacheco F, Coelho R, Leit xe3 o D, Ricardo S, David L. Mesothelin Expression Is Not Associated with the Presence of Cancer Stem Cell Markers SOX2 and ALDH1 in Ovarian Cancer. Int J Mol Sci. 2022;23: pubmed publisher
  3. Coelho R, Ricardo S, Amaral A, Huang Y, Nunes M, Neves J, et al. Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation. Oncogenesis. 2020;9:61 pubmed publisher
  4. Watanabe K, Luo Y, Da T, Guedan S, Ruella M, Scholler J, et al. Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses. JCI Insight. 2018;3: pubmed publisher
  5. Smith T, Moffett H, Stephan S, Opel C, Dumigan A, Jiang X, et al. Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors. J Clin Invest. 2017;127:2176-2191 pubmed publisher
  6. De Santi C, Vencken S, Blake J, Haase B, Benes V, Gemignani F, et al. Identification of MiR-21-5p as a Functional Regulator of Mesothelin Expression Using MicroRNA Capture Affinity Coupled with Next Generation Sequencing. PLoS ONE. 2017;12:e0170999 pubmed publisher
  7. Awuah P, Bera T, Folivi M, Chertov O, Pastan I. Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins. Mol Cancer Ther. 2016;15:1648-55 pubmed publisher
  8. Ibrahim D, Abouhashem N. Diagnostic value of IMP3 and mesothelin in differentiating pancreatic ductal adenocarcinoma from chronic pancreatitis. Pathol Res Pract. 2016;212:288-93 pubmed publisher
  9. Wang E, Wang L, Tsai C, Bhoj V, Gershenson Z, Moon E, et al. Generation of Potent T-cell Immunotherapy for Cancer Using DAP12-Based, Multichain, Chimeric Immunoreceptors. Cancer Immunol Res. 2015;3:815-26 pubmed publisher
  10. Ordonez N, Sahin A. Diagnostic utility of immunohistochemistry in distinguishing between epithelioid pleural mesotheliomas and breast carcinomas: a comparative study. Hum Pathol. 2014;45:1529-40 pubmed publisher