This is a Validated Antibody Database (VAD) review about human matriptase, based on 4 published articles (read how Labome selects the articles), using matriptase antibody in all methods. It is aimed to help Labome visitors find the most suited matriptase antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
matriptase synonym: ARCI11; HAI; MT-SP1; MTSP1; PRSS14; SNC19; TADG15; TMPRSS14; suppressor of tumorigenicity 14 protein; membrane-type serine protease 1; prostamin; serine protease 14; serine protease TADG-15; suppression of tumorigenicity 14 (colon carcinoma); suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); tumor associated differentially expressed gene 15 protein

R&D Systems
sheep polyclonal
  • immunocytochemistry; human; loading ...; fig 2b
  • western blot; human; loading ...; fig 6c
In order to test if HAI-2, matriptase, EpCAM, and claudin-7 are functionally linked in the intestine, R&D Systems matriptase antibody (R&D Systems, AF3946) was used in immunocytochemistry on human samples (fig 2b) and in western blot on human samples (fig 6c). J Clin Invest (2017) ncbi
sheep polyclonal
  • western blot; human
  • immunohistochemistry; mouse
  • western blot; mouse
In order to determine the effect of ablating HAI-1 and HAI-2 on endogenous matriptase expression, subcellular localization, and activation in polarized intestinal epithelial cells, R&D Systems matriptase antibody (R&D Systems, AF3946) was used in western blot on human samples , in immunohistochemistry on mouse samples and in western blot on mouse samples . J Biol Chem (2014) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; human; 1:500; fig 1
  • western blot; human; 1:1000; fig 2
Abcam matriptase antibody (abcam, ab28266) was used in immunocytochemistry on human samples at 1:500 (fig 1) and in western blot on human samples at 1:1000 (fig 2). Mol Med Rep (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 1e
In order to elucidate the contribution of SPRY2 to colon cancer, Bethyl matriptase antibody (Bethyl, A300-221A) was used in western blot on human samples (fig 1e). Oncogene (2016) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2b
  • western blot; human; loading ...; fig 6c
In order to test if HAI-2, matriptase, EpCAM, and claudin-7 are functionally linked in the intestine, EMD Millipore matriptase antibody (EMD Biosciences, IM1014) was used in immunocytochemistry on human samples (fig 2b) and in western blot on human samples (fig 6c). J Clin Invest (2017) ncbi
Articles Reviewed
  1. Wu C, Feng X, Lu M, Morimura S, Udey M. Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis. J Clin Invest. 2017;127:623-634 pubmed publisher
  2. Sun P, Jiang Z, Chen X, Xue L, Mao X, Ruan G, et al. Decreasing the ratio of matriptase/HAI?1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep. 2016;14:1465-74 pubmed publisher
  3. Barbáchano A, Fernández Barral A, Pereira F, Segura M, Ordóñez Morán P, Carrillo de Santa Pau E, et al. SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150. Oncogene. 2016;35:2991-3003 pubmed publisher
  4. Friis S, Sales K, Schafer J, Vogel L, Kataoka H, Bugge T. The protease inhibitor HAI-2, but not HAI-1, regulates matriptase activation and shedding through prostasin. J Biol Chem. 2014;289:22319-32 pubmed publisher