This is a Validated Antibody Database (VAD) review about human integrin alpha5, based on 59 published articles (read how Labome selects the articles), using integrin alpha5 antibody in all methods. It is aimed to help Labome visitors find the most suited integrin alpha5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
integrin alpha5 synonym: CD49e; FNRA; VLA-5; VLA5A

Knockout validation
BD Biosciences
mouse monoclonal (1/CD49e)
  • western blot; Chinese hamsters; fig 2
  • western blot knockout validation; human; fig 6
BD Biosciences integrin alpha5 antibody (BD Biosciences;, 610634) was used in western blot on Chinese hamsters samples (fig 2) and in western blot knockout validation on human samples (fig 6). Sci Rep (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-9)
  • western blot; human; 1:2000; loading ...; fig 6a
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz, sc-376199) was used in western blot on human samples at 1:2000 (fig 6a). iScience (2022) ncbi
mouse monoclonal (C-9)
  • immunohistochemistry; human; 1:100; loading ...; fig s6-1a
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-376199) was used in immunohistochemistry on human samples at 1:100 (fig s6-1a). elife (2021) ncbi
mouse monoclonal (C-9)
  • immunohistochemistry; human; 1:100; loading ...; fig s6-1a
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-376199) was used in immunohistochemistry on human samples at 1:100 (fig s6-1a). elife (2021) ncbi
mouse monoclonal (C-9)
  • western blot; human; 1:500; loading ...
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, SC-376199) was used in western blot on human samples at 1:500. Commun Biol (2021) ncbi
mouse monoclonal (C-9)
  • western blot; human; 1:200; loading ...; fig 2b
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-376199) was used in western blot on human samples at 1:200 (fig 2b). elife (2020) ncbi
mouse monoclonal (A-11)
  • western blot; human; 1:200; loading ...; fig 5a
Santa Cruz Biotechnology integrin alpha5 antibody (SantaCruz, sc-166665) was used in western blot on human samples at 1:200 (fig 5a). Oncotarget (2016) ncbi
mouse monoclonal (A-11)
  • western blot; human
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-166665) was used in western blot on human samples . Colloids Surf B Biointerfaces (2015) ncbi
mouse monoclonal (1)
  • western blot; human; 1:200
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-136224) was used in western blot on human samples at 1:200. Oncotarget (2015) ncbi
mouse monoclonal (JBS5)
  • blocking or activating experiments; human; 2 ug 1:25
Santa Cruz Biotechnology integrin alpha5 antibody (Santa Cruz Biotechnology, sc-59762) was used in blocking or activating experiments on human samples at 2 ug 1:25. Biomacromolecules (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR7854)
  • immunohistochemistry; human; 1:100; loading ...; fig 3e
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in immunohistochemistry on human samples at 1:100 (fig 3e). Cell Biosci (2021) ncbi
domestic rabbit monoclonal (EPR7854)
  • western blot; human; 1:1000; loading ...; fig 8e
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in western blot on human samples at 1:1000 (fig 8e). J Cell Biol (2021) ncbi
domestic rabbit monoclonal
  • immunohistochemistry; mouse; loading ...
Abcam integrin alpha5 antibody (abcam, ab221606) was used in immunohistochemistry on mouse samples . Nat Commun (2021) ncbi
domestic rabbit monoclonal (EPR7854)
  • western blot; bovine; loading ...; fig s2b
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in western blot on bovine samples (fig s2b). JCI Insight (2020) ncbi
domestic rabbit monoclonal (EPR7854)
  • immunohistochemistry; human; 1:100; loading ...; fig 4g
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in immunohistochemistry on human samples at 1:100 (fig 4g). Dev Cell (2020) ncbi
domestic rabbit monoclonal (EPR7854)
  • immunohistochemistry - paraffin section; human; loading ...; fig 6, t2
Abcam integrin alpha5 antibody (Abcam, EPR7854) was used in immunohistochemistry - paraffin section on human samples (fig 6, t2). Cancers (Basel) (2019) ncbi
domestic rabbit monoclonal (EPR7854)
  • immunohistochemistry; human; loading ...; fig 2g
  • western blot; human; loading ...; fig 2i
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in immunohistochemistry on human samples (fig 2g) and in western blot on human samples (fig 2i). J Exp Med (2019) ncbi
domestic rabbit monoclonal (EPR7854)
  • western blot; human; 1:1000; loading ...; fig 3a
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in western blot on human samples at 1:1000 (fig 3a). J Clin Invest (2019) ncbi
domestic rabbit monoclonal (EPR7854)
  • immunocytochemistry; human; loading ...; fig s5a
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in immunocytochemistry on human samples (fig s5a). Mol Biol Cell (2017) ncbi
domestic rabbit monoclonal (EPR7854)
  • immunocytochemistry; human; loading ...; fig s2d
Abcam integrin alpha5 antibody (Abcam, ab150361) was used in immunocytochemistry on human samples (fig s2d). J Cell Biol (2016) ncbi
BioLegend
mouse monoclonal (NKI-SAM-1)
  • flow cytometry; human
BioLegend integrin alpha5 antibody (BioLegend, 328011) was used in flow cytometry on human samples . Front Immunol (2022) ncbi
mouse monoclonal (NKI-SAM-1)
  • flow cytometry; human; fig 6a
In order to investigate the protective effect of single domain camelid antibodies against botulinum neurotoxin, BioLegend integrin alpha5 antibody (biolegend, 328012) was used in flow cytometry on human samples (fig 6a). Nat Commun (2017) ncbi
mouse monoclonal (NKI-SAM-1)
  • flow cytometry; human; fig 4
In order to determine modulation of presentation of cell surfaced calreticulin by alpha-integrin function and expression, BioLegend integrin alpha5 antibody (Biolegend, NKI-SAM-1) was used in flow cytometry on human samples (fig 4). Cell Death Dis (2016) ncbi
mouse monoclonal (NKI-SAM-1)
  • flow cytometry; human; fig 2
BioLegend integrin alpha5 antibody (Biolegend, 328008) was used in flow cytometry on human samples (fig 2). Methods Mol Biol (2016) ncbi
mouse monoclonal (NKI-SAM-1)
  • flow cytometry; human; fig 3
In order to study the role of alpha-4 integrin in the mechanism by which CD82 modulates cell adhesion, BioLegend integrin alpha5 antibody (BioLegend, NKI-SAM-1) was used in flow cytometry on human samples (fig 3). Mol Biol Cell (2014) ncbi
Invitrogen
mouse monoclonal (eBioSAM-1 (SAM-1, SAM1))
  • flow cytometry; human; 1:50; fig 3a, 3b
Invitrogen integrin alpha5 antibody (eBioscience/Thermo, 53-0496-42) was used in flow cytometry on human samples at 1:50 (fig 3a, 3b). Stem Cells (2019) ncbi
mouse monoclonal (P1D6)
  • immunocytochemistry; human; loading ...; fig s2a
In order to describe the crosstalk of alphavbeta6-integrin and androgen receptor in prostate cancer., Invitrogen integrin alpha5 antibody (Life Technologies, P1D6) was used in immunocytochemistry on human samples (fig s2a). Cancer Res (2016) ncbi
mouse monoclonal (10F6)
  • immunohistochemistry; human; fig 6
  • western blot; human; fig 3
In order to investigate how integrins regulate adipose tissue stem cell proliferation and differentiation, Invitrogen integrin alpha5 antibody (Thermo Scientific, MA5-15568) was used in immunohistochemistry on human samples (fig 6) and in western blot on human samples (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (eBioSAM-1 (SAM-1, SAM1))
  • flow cytometry; mouse; 1:300; fig 6
  • western blot; mouse; 20 ug/ml; fig 6
Invitrogen integrin alpha5 antibody (eBioscience, NKI-SAM-1) was used in flow cytometry on mouse samples at 1:300 (fig 6) and in western blot on mouse samples at 20 ug/ml (fig 6). Nat Commun (2016) ncbi
Novus Biologicals
mouse monoclonal (SNAKA51)
  • immunocytochemistry; human; loading ...; fig 2c
Novus Biologicals integrin alpha5 antibody (Novus, NBP250146) was used in immunocytochemistry on human samples (fig 2c). Curr Biol (2020) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2c
Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in western blot on human samples at 1:1000 (fig 2c). Oncogenesis (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4c
Cell Signaling Technology integrin alpha5 antibody (Cell Signaling Technology, 4705) was used in western blot on human samples at 1:1000 (fig 4c). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s5b
In order to research the promotional effect of endoglin on small muscle cell migration and vascular maturation, Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in western blot on human samples at 1:1000 (fig s5b). Arterioscler Thromb Vasc Biol (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s2a
In order to measure the adhesion of alphaV-class and/or alpha5beta1 integrins expressing fibroblasts initiating attachment to fibronectin, Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in western blot on mouse samples at 1:1000 (fig s2a). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig 3b
In order to find that mice with a dysfunctional fibronectin-synergy motif suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury, Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in western blot on mouse samples at 1:2000 (fig 3b). elife (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 2
In order to study the dependence on host integrins, membrane raft recruitment, and Src-family kinase activation for histoplasma capsulatum-induced cytokine secretion in lung epithelial cells, Cell Signaling Technology integrin alpha5 antibody (Cell signaling, 4705) was used in western blot on human samples at 1:1000 (fig 2). Front Microbiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 2s2
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in western blot on mouse samples at 1:1000 (fig 2s2). elife (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:10; fig 5
  • western blot; human; 1:1000; fig 1a
In order to determine immune responses to Paracoccidioides brasiliensis, Cell Signaling Technology integrin alpha5 antibody (Cell Signaling, 4705) was used in immunocytochemistry on human samples at 1:10 (fig 5) and in western blot on human samples at 1:1000 (fig 1a). Microbes Infect (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 2a
Cell Signaling Technology integrin alpha5 antibody (Cell Signaling Technology, 4705) was used in western blot on human samples (fig 2a). Oncogene (2016) ncbi
Beckman Coulter
mouse monoclonal (SAM1)
  • flow cytometry; African green monkey; loading ...; fig 1a
In order to assess the role of host membrane trafficking to latent Epstein-Barr virus infection of B cells, Beckman Coulter integrin alpha5 antibody (Beckman Coulter, SAM1) was used in flow cytometry on African green monkey samples (fig 1a). J Gen Virol (2016) ncbi
mouse monoclonal (SAM1)
  • other; human; 200 ug/ml; fig 1
Beckman Coulter integrin alpha5 antibody (Beckman Coulter, IM0771) was used in other on human samples at 200 ug/ml (fig 1). J Extracell Vesicles (2016) ncbi
BD Biosciences
mouse monoclonal (VC5)
BD Biosciences integrin alpha5 antibody (BD Pharmingen, VC5) was used . Angiogenesis (2021) ncbi
mouse monoclonal (1/CD49e)
  • immunocytochemistry; human; loading ...; fig 2b
BD Biosciences integrin alpha5 antibody (BD, 610633) was used in immunocytochemistry on human samples (fig 2b). Curr Biol (2020) ncbi
mouse monoclonal (VC5)
BD Biosciences integrin alpha5 antibody (BD, 555651) was used . Curr Biol (2020) ncbi
mouse monoclonal (1/CD49e)
  • western blot; human; 1:1000; loading ...; fig 1a
In order to elucidate the mechanisms by which Rab-coupling protein contributes to metastasis, BD Biosciences integrin alpha5 antibody (BD Transduction Labs, 610633) was used in western blot on human samples at 1:1000 (fig 1a). Nat Commun (2017) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human; 1:50; loading ...; tbl 1
BD Biosciences integrin alpha5 antibody (Becton, 555617) was used in flow cytometry on human samples at 1:50 (tbl 1). Sci Rep (2017) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human; loading ...; fig 2d
BD Biosciences integrin alpha5 antibody (BD, IIA1) was used in flow cytometry on human samples (fig 2d). Int J Mol Sci (2017) ncbi
mouse monoclonal (VC5)
  • flow cytometry; human; fig 2a
In order to see if tetraspanin 8 influences cell-matrix anchorage and regulates downstream molecular pathways leading to aggressive behavior of tumor progression in melanoma, BD Biosciences integrin alpha5 antibody (BD Pharmingen, VC5) was used in flow cytometry on human samples (fig 2a). Oncotarget (2017) ncbi
mouse monoclonal (1/CD49e)
  • western blot; Chinese hamsters; fig 2
  • western blot knockout validation; human; fig 6
BD Biosciences integrin alpha5 antibody (BD Biosciences;, 610634) was used in western blot on Chinese hamsters samples (fig 2) and in western blot knockout validation on human samples (fig 6). Sci Rep (2016) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human; loading ...; fig 5c
In order to investigate the effect of oral keratinocyte cell cycle on Porphyromonas gingivalis infection, BD Biosciences integrin alpha5 antibody (BD Pharmingen, IIA1) was used in flow cytometry on human samples (fig 5c). Infect Immun (2016) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences integrin alpha5 antibody (BD, 555617) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (1/CD49e)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences integrin alpha5 antibody (BD, 1) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human
In order to identify the cell surface markers in synovial mesenchymal stem cells, BD Biosciences integrin alpha5 antibody (BD Pharmingen, 555617) was used in flow cytometry on human samples . Cytometry A (2015) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human
BD Biosciences integrin alpha5 antibody (BD Biosciences, IIA1) was used in flow cytometry on human samples . Neoplasia (2014) ncbi
mouse monoclonal (1/CD49e)
  • western blot; human
BD Biosciences integrin alpha5 antibody (BD, 610633) was used in western blot on human samples . BMC Cancer (2013) ncbi
mouse monoclonal (IIA1)
  • flow cytometry; human; 1:100
In order to evaluate a cell culture system for long-term passaging of human pluripotent stem cells, BD Biosciences integrin alpha5 antibody (BD Biosciences, 555617) was used in flow cytometry on human samples at 1:100. J Neurosci Res (2013) ncbi
mouse monoclonal (VC5)
  • flow cytometry; human
In order to show that BRAG2 acts at clathrin-coated pits to promote integrin internalization by activating Arf5, BD Biosciences integrin alpha5 antibody (BD Pharmigen, 555651) was used in flow cytometry on human samples . J Biol Chem (2012) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (P1D6)
  • blocking or activating experiments; human; loading ...; fig 7c
Developmental Studies Hybridoma Bank integrin alpha5 antibody (Developmental Studies Hybridoma Bank, P1D6) was used in blocking or activating experiments on human samples (fig 7c). JCI Insight (2021) ncbi
mouse monoclonal (P1D6)
  • blocking or activating experiments; human; 10 ug/ml; loading ...; fig 6c
Developmental Studies Hybridoma Bank integrin alpha5 antibody (Merck, P1D6) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 6c). Oncogene (2021) ncbi
mouse monoclonal (P1D6)
  • immunoprecipitation; African green monkey; loading ...; fig 3d
  • immunoprecipitation; human; loading ...; fig 3b
In order to investigate TGF-beta-mediated fibrillogenesis, Developmental Studies Hybridoma Bank integrin alpha5 antibody (DSHB, P1D6) was used in immunoprecipitation on African green monkey samples (fig 3d) and in immunoprecipitation on human samples (fig 3b). Mol Biol Cell (2017) ncbi
mouse monoclonal (P1D6)
  • blocking or activating experiments; human; loading ...; fig 6C
In order to investigate the crosstalk among MenaINV, RTKs, and integrins, Developmental Studies Hybridoma Bank integrin alpha5 antibody (DSHB, P1D6) was used in blocking or activating experiments on human samples (fig 6C). Mol Biol Cell (2016) ncbi
mouse monoclonal (PB1)
  • blocking or activating experiments; hamsters; fig 4
  • flow cytometry; hamsters; 1:50; fig 4
Developmental Studies Hybridoma Bank integrin alpha5 antibody (Developmental Studies Hybridoma Bank, PB1) was used in blocking or activating experiments on hamsters samples (fig 4) and in flow cytometry on hamsters samples at 1:50 (fig 4). Nat Commun (2015) ncbi
Articles Reviewed
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  39. Maza P, Suzuki E. Histoplasma capsulatum-Induced Cytokine Secretion in Lung Epithelial Cells Is Dependent on Host Integrins, Src-Family Kinase Activation, and Membrane Raft Recruitment. Front Microbiol. 2016;7:580 pubmed publisher
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