This is a Validated Antibody Database (VAD) review about human huntingtin, based on 14 published articles (read how Labome selects the articles), using huntingtin antibody in all methods. It is aimed to help Labome visitors find the most suited huntingtin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
huntingtin synonym: HD; IT15; LOMARS

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5526)
  • western blot knockout validation; mouse; loading ...; fig 3c
Abcam huntingtin antibody (Abcam, EPR5526) was used in western blot knockout validation on mouse samples (fig 3c). Proc Natl Acad Sci U S A (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR5526)
  • western blot; mouse; 1:5000; loading ...; fig 5b
Abcam huntingtin antibody (Abcam, ab109115) was used in western blot on mouse samples at 1:5000 (fig 5b). PLoS Genet (2017) ncbi
domestic rabbit monoclonal (EPR5526)
  • western blot; mouse; 1:3000; loading ...; fig 3c
In order to discuss regulation of mutant huntingtin by transcription factor EB, Abcam huntingtin antibody (Abcam, EPR5526) was used in western blot on mouse samples at 1:3000 (fig 3c). J Huntingtons Dis (2016) ncbi
domestic rabbit monoclonal (EPR5526)
  • western blot; mouse; 1:3000; loading ...; fig 6a
In order to test if increased NUB1 protein levels reduces mutant huntingtin levels through a proteasome dependent mechanism, Abcam huntingtin antibody (Abcam, EPR5526) was used in western blot on mouse samples at 1:3000 (fig 6a). J Huntingtons Dis (2016) ncbi
domestic rabbit monoclonal (EPR5526)
  • western blot knockout validation; mouse; loading ...; fig 3c
Abcam huntingtin antibody (Abcam, EPR5526) was used in western blot knockout validation on mouse samples (fig 3c). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (EP867Y)
  • flow cytometry; mouse; fig s1
Abcam huntingtin antibody (Abcam, EP867Y) was used in flow cytometry on mouse samples (fig s1). Sci Rep (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3E10)
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 6a
  • immunocytochemistry; mouse; loading ...; fig 6m
Santa Cruz Biotechnology huntingtin antibody (Santa Cruz, sc-47757) was used in immunohistochemistry - free floating section on mouse samples at 1:500 (fig 6a) and in immunocytochemistry on mouse samples (fig 6m). Nat Neurosci (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D7F7)
  • western blot; mouse; loading ...; fig 4a
Cell Signaling Technology huntingtin antibody (Cell Signaling, 5656) was used in western blot on mouse samples (fig 4a). elife (2020) ncbi
domestic rabbit monoclonal (D7F7)
  • western blot; mouse; 1:1000; loading ...; fig ev1c
Cell Signaling Technology huntingtin antibody (Cell signaling, D7F7) was used in western blot on mouse samples at 1:1000 (fig ev1c). EMBO Mol Med (2020) ncbi
domestic rabbit monoclonal (D7F7)
  • western blot; human; 1:1000; loading ...; fig 1b
Cell Signaling Technology huntingtin antibody (Cell Signaling, D7F7) was used in western blot on human samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (MW7)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 7a
Developmental Studies Hybridoma Bank huntingtin antibody (DSHB, MW7) was used in immunohistochemistry on mouse samples at 1:1000 (fig 7a). Nat Commun (2020) ncbi
mouse monoclonal (MW8)
  • immunocytochemistry; human; 1:100; loading ...; fig 4i
Developmental Studies Hybridoma Bank huntingtin antibody (DSHB, MW8) was used in immunocytochemistry on human samples at 1:100 (fig 4i). Nat Neurosci (2018) ncbi
mouse monoclonal (MW8)
  • immunohistochemistry; mouse; 1:750; fig 4a
Developmental Studies Hybridoma Bank huntingtin antibody (DSHB, MW8) was used in immunohistochemistry on mouse samples at 1:750 (fig 4a). Sci Rep (2017) ncbi
mouse monoclonal (MW1)
  • western blot; human; 1:500
Developmental Studies Hybridoma Bank huntingtin antibody (Developmental Studies Hybridoma Bank, MW1) was used in western blot on human samples at 1:500. PLoS ONE (2014) ncbi
mouse monoclonal (MW7)
  • immunohistochemistry; human; 1:100
Developmental Studies Hybridoma Bank huntingtin antibody (Developmental Studies Hybridoma Bank, MW7) was used in immunohistochemistry on human samples at 1:100. Ann Neurol (2014) ncbi
Articles Reviewed
  1. Bruyère J, Abada Y, Vitet H, Fontaine G, Deloulme J, Ces A, et al. Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of huntingtin. elife. 2020;9: pubmed publisher
  2. Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
  3. Ehinger Y, Bruyère J, Panayotis N, Abada Y, Borloz E, Matagne V, et al. Huntingtin phosphorylation governs BDNF homeostasis and improves the phenotype of Mecp2 knockout mice. EMBO Mol Med. 2020;12:e10889 pubmed publisher
  4. Shen M, Wang F, Li M, Sah N, Stockton M, Tidei J, et al. Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice. Nat Neurosci. 2019;22:386-400 pubmed publisher
  5. Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
  6. Bragg R, Coffey S, Weston R, Ament S, Cantle J, Minnig S, et al. Motivational, proteostatic and transcriptional deficits precede synapse loss, gliosis and neurodegeneration in the B6.HttQ111/+ model of Huntington's disease. Sci Rep. 2017;7:41570 pubmed publisher
  7. Nakasuji T, Ogonuki N, Chiba T, Kato T, Shiozawa K, Yamatoya K, et al. Complementary Critical Functions of Zfy1 and Zfy2 in Mouse Spermatogenesis and Reproduction. PLoS Genet. 2017;13:e1006578 pubmed publisher
  8. Vodicka P, Chase K, Iuliano M, Tousley A, Valentine D, Sapp E, et al. Autophagy Activation by Transcription Factor EB (TFEB) in Striatum of HDQ175/Q7 Mice. J Huntingtons Dis. 2016;5:249-260 pubmed
  9. Vodicka P, Chase K, Iuliano M, Valentine D, Sapp E, Lu B, et al. Effects of Exogenous NUB1 Expression in the Striatum of HDQ175/Q7 Mice. J Huntingtons Dis. 2016;5:163-74 pubmed publisher
  10. Wang G, Liu X, Gaertig M, Li S, Li X. Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis. Proc Natl Acad Sci U S A. 2016;113:3359-64 pubmed publisher
  11. Lopes C, Aubert S, Bourgois Rocha F, Barnat M, Rego A, Déglon N, et al. Dominant-Negative Effects of Adult-Onset Huntingtin Mutations Alter the Division of Human Embryonic Stem Cells-Derived Neural Cells. PLoS ONE. 2016;11:e0148680 pubmed publisher
  12. Southwell A, Smith S, Davis T, Caron N, Villanueva E, Xie Y, et al. Ultrasensitive measurement of huntingtin protein in cerebrospinal fluid demonstrates increase with Huntington disease stage and decrease following brain huntingtin suppression. Sci Rep. 2015;5:12166 pubmed publisher
  13. Macdonald D, Tessari M, Boogaard I, Smith M, Pulli K, Szynol A, et al. Quantification assays for total and polyglutamine-expanded huntingtin proteins. PLoS ONE. 2014;9:e96854 pubmed publisher
  14. Cicchetti F, Lacroix S, Cisbani G, Vallières N, Saint Pierre M, St Amour I, et al. Mutant huntingtin is present in neuronal grafts in Huntington disease patients. Ann Neurol. 2014;76:31-42 pubmed publisher