This is a Validated Antibody Database (VAD) review about human furin, based on 11 published articles (read how Labome selects the articles), using furin antibody in all methods. It is aimed to help Labome visitors find the most suited furin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
furin synonym: FUR; PACE; PCSK3; SPC1; furin; FES upstream region; FURIN/ADAMTS1 fusion; dibasic processing enzyme; furin, membrane associated receptor protein; paired basic amino acid residue-cleaving enzyme; proprotein convertase subtilisin/kexin type 3

Abcam
rabbit polyclonal
  • western blot; human; 1:1500
In order to investigate the localization and binding partners of the tyrosyl protein sulfotransferases, TPST1 and TPST2, Abcam furin antibody (abcam, ab3467) was used in western blot on human samples at 1:1500. J Mol Biol (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human
Abcam furin antibody (Abcam, ab3467) was used in immunocytochemistry on human samples and in western blot on human samples . Placenta (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human
Abcam furin antibody (Abcam, ab3467) was used in immunocytochemistry on human samples and in western blot on human samples . Exp Cell Res (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Abcam furin antibody (Abcam, ab3467) was used in immunocytochemistry on human samples . Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam furin antibody (Abcam, ab3467) was used in western blot on mouse samples . Cell Death Dis (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:600
Abcam furin antibody (Abcam, ab3467) was used in immunohistochemistry on rat samples at 1:600. PLoS ONE (2013) ncbi
R&D Systems
mouse monoclonal (222712)
  • western blot; hamsters; 0.1 ug/ml; fig s4
In order to evaluate a novel approach for generating bispecific monovalent antibodies, R&D Systems furin antibody (R& D System, MAB15032-SP) was used in western blot on hamsters samples at 0.1 ug/ml (fig s4). MAbs (2017) ncbi
goat polyclonal
  • flow cytometry; human; fig 6
R&D Systems furin antibody (R&D Systems, AF1503) was used in flow cytometry on human samples (fig 6). J Biol Chem (2015) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 9c
  • western blot; rat; 1:500; fig 10
  • western blot; African green monkey; 1:500; loading ...; fig 7c
In order to use antibodies to differentiate subtypes of microglia, Invitrogen furin antibody (Affinity BioReagents, PA1-062) was used in western blot on human samples at 1:500 (fig 9c), in western blot on rat samples at 1:500 (fig 10) and in western blot on African green monkey samples at 1:500 (fig 7c). Glia (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 2
In order to examine the osteoblast to osteocyte transition in regards to posttranslational processing, Invitrogen furin antibody (Affinity Bioreagents, PA1-062) was used in immunocytochemistry on human samples at 1:100 (fig 2). Bone (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:50
  • western blot; human; 1:1000
In order to study endogenous activin A in human granulosa cells, Invitrogen furin antibody (Thermo Scientific, PA1-062) was used in immunocytochemistry on human samples at 1:50 and in western blot on human samples at 1:1000. J Clin Endocrinol Metab (2015) ncbi
Articles Reviewed
  1. Dimasi N, Fleming R, SACHSENMEIER K, Bezabeh B, Hay C, Wu J, et al. Guiding bispecific monovalent antibody formation through proteolysis of IgG1 single-chain. MAbs. 2017;9:438-454 pubmed publisher
  2. Kawahara K, Hirata H, Ohbuchi K, Nishi K, Maeda A, Kuniyasu A, et al. The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin-like protease(s) in a subpopulation of microglia in neonatal rat brain. Glia. 2016;64:1938-61 pubmed publisher
  3. Yamamoto H, Ramos Molina B, Lick A, Prideaux M, Albornoz V, Bonewald L, et al. Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition. Bone. 2016;84:120-130 pubmed publisher
  4. Huygens C, Liénart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, et al. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor β1 (TGF-β1) Production in Human Regulatory T Cells. J Biol Chem. 2015;290:20105-16 pubmed publisher
  5. Hartmann Fatu C, Trusch F, Moll C, Michin I, Hassinen A, Kellokumpu S, et al. Heterodimers of tyrosylprotein sulfotransferases suggest existence of a higher organization level of transferases in the membrane of the trans-Golgi apparatus. J Mol Biol. 2015;427:1404-12 pubmed publisher
  6. Chang H, Cheng J, Klausen C, Leung P. Recombinant BMP4 and BMP7 increase activin A production by up-regulating inhibin βA subunit and furin expression in human granulosa-lutein cells. J Clin Endocrinol Metab. 2015;100:E375-86 pubmed publisher
  7. Zhou Z, Wang R, Yang X, Lu X, Zhang Q, Wang Y, et al. The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization. Placenta. 2014;35:907-18 pubmed publisher
  8. Sadeqzadeh E, de Bock C, Wojtalewicz N, Holt J, Smith N, Dun M, et al. Furin processing dictates ectodomain shedding of human FAT1 cadherin. Exp Cell Res. 2014;323:41-55 pubmed publisher
  9. Burgert T, Schmidt V, Caglayan S, Lin F, Füchtbauer A, Füchtbauer E, et al. SORLA-dependent and -independent functions for PACS1 in control of amyloidogenic processes. Mol Cell Biol. 2013;33:4308-20 pubmed publisher
  10. Zhou Z, Zhang Q, Lu X, Wang R, Wang Y, Zhu C, et al. The proprotein convertase furin is required for trophoblast syncytialization. Cell Death Dis. 2013;4:e593 pubmed publisher
  11. Yang X, Wang Q, Gao Z, Zhou Z, Peng S, Chang W, et al. Proprotein convertase furin regulates apoptosis and proliferation of granulosa cells in the rat ovary. PLoS ONE. 2013;8:e50479 pubmed publisher