This is a Validated Antibody Database (VAD) review about human emerin, based on 14 published articles (read how Labome selects the articles), using emerin antibody in all methods. It is aimed to help Labome visitors find the most suited emerin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
emerin synonym: EDMD; LEMD5; STA

Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 2a
Abcam emerin antibody (Abcam, ab40688) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 2a). Mol Cell Biochem (2021) ncbi
domestic rabbit monoclonal (EPR11071)
  • western blot; human; loading ...; fig 3b
Abcam emerin antibody (Abcam, ab156871) was used in western blot on human samples (fig 3b). Science (2020) ncbi
domestic rabbit monoclonal (EPR11071)
  • western blot; human; loading ...; fig 3b
Abcam emerin antibody (Abcam, ab156871) was used in western blot on human samples (fig 3b). Cell (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-12)
  • immunocytochemistry; human; loading ...; fig 5a
  • western blot; human; 1:3000; loading ...; fig 6a
Santa Cruz Biotechnology emerin antibody (Santa Cruz, sc-25284) was used in immunocytochemistry on human samples (fig 5a) and in western blot on human samples at 1:3000 (fig 6a). Cells (2020) ncbi
mouse monoclonal (H-12)
  • western blot; human; 1:500
In order to describe a method to investigate protein interactions in the nuclear envelope, Santa Cruz Biotechnology emerin antibody (Santa Cruz Biotechnology, sc-25284) was used in western blot on human samples at 1:500. Biochim Biophys Acta (2014) ncbi
Novus Biologicals
mouse monoclonal (CL0201)
  • immunohistochemistry; human; 1:500; loading ...; fig 4b, 4d
Novus Biologicals emerin antibody (Novus Biologicals, CL0201) was used in immunohistochemistry on human samples at 1:500 (fig 4b, 4d). Oncol Rep (2019) ncbi
domestic rabbit polyclonal (OTI7A4)
  • western blot; hamsters; loading ...; fig s19a
In order to propose that local stresses applied to integrins propagate from the tensed actin cytoskeleton to the LINC complex and then through lamina-chromatin interactions to directly stretch chromatin and upregulate transcription, Novus Biologicals emerin antibody (Novus biological, NBP1-87692) was used in western blot on hamsters samples (fig s19a). Nat Mater (2016) ncbi
ImmuQuest
mouse monoclonal (JOL2)
  • immunohistochemistry; human; fig 1
ImmuQuest emerin antibody (Immuquest, Jol2) was used in immunohistochemistry on human samples (fig 1). Aging Cell (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s1c
Cell Signaling Technology emerin antibody (Cell Signaling, 2659S) was used in western blot on human samples at 1:1000 (fig s1c). J Cell Biol (2019) ncbi
Leica Biosystems
mouse monoclonal (4G5)
  • western blot; human; 1:300; loading ...; fig 4b
In order to determine the cause of early-onset myopathy with internalized nuclei and myofibrillar disorganization, Leica Biosystems emerin antibody (Novocastra, NCL) was used in western blot on human samples at 1:300 (fig 4b). Am J Hum Genet (2016) ncbi
mouse monoclonal (4G5)
  • immunocytochemistry; human; 1:500; fig 2
  • western blot; human; 1:500; fig 9
In order to test if progerin elicits spatiotemporal deviations in mitotic processes in Hutchinson-Gilford progeria syndrome fibroblasts, Leica Biosystems emerin antibody (Leica Biosystems, NCL-Emerin) was used in immunocytochemistry on human samples at 1:500 (fig 2) and in western blot on human samples at 1:500 (fig 9). Oncotarget (2016) ncbi
mouse monoclonal (4G5)
  • immunocytochemistry; mouse; 1:50; fig s2d
In order to study modulation of Nkx2.5 expression by participating in transcription factor complexes that interact with nkx2.5 after desmin enters the nucleus of cardiac stem cells, Leica Biosystems emerin antibody (Novocastra, NCL-Emerin) was used in immunocytochemistry on mouse samples at 1:50 (fig s2d). Biol Open (2016) ncbi
mouse monoclonal (4G5)
  • western blot; mouse; 1:5000
In order to characterize mice harboring a disrupted allele for the Lem2 gene, Leica Biosystems emerin antibody (Novocastra, NCL-EMERIN) was used in western blot on mouse samples at 1:5000. PLoS ONE (2015) ncbi
mouse monoclonal (4G5)
  • immunohistochemistry; mouse; 1:200
In order to investigate the role of B-type lamins in the maintenance of nuclear integrity of keratinocytes, Leica Biosystems emerin antibody (Novocastra, NCL-EMERIN) was used in immunohistochemistry on mouse samples at 1:200. Mol Cell Biol (2014) ncbi
Articles Reviewed
  1. Volkening K, Farhan S, Kao J, Leystra Lantz C, Ang L, McIntyre A, et al. Evidence of synergism among three genetic variants in a patient with LMNA-related lipodystrophy and amyotrophic lateral sclerosis leading to a remarkable nuclear phenotype. Mol Cell Biochem. 2021;476:2633-2650 pubmed publisher
  2. Svobodová Kovaříková A, Bartova E, Kovarik A, Lukasova E. Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in γ-Irradiation-Induced Senescent Cells. Cells. 2020;9: pubmed publisher
  3. Topalian S, Taube J, Pardoll D. Neoadjuvant checkpoint blockade for cancer immunotherapy. Science. 2020;367: pubmed publisher
  4. Halfmann C, Sears R, Katiyar A, Busselman B, Aman L, Zhang Q, et al. Repair of nuclear ruptures requires barrier-to-autointegration factor. J Cell Biol. 2019;: pubmed publisher
  5. Kobayashi S, Saio M, Fukuda T, Kimura K, Hirato J, Oyama T. Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncol Rep. 2019;41:133-142 pubmed publisher
  6. Mirza A, McKellar S, Urman N, Brown A, Hollmig T, Aasi S, et al. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription. Cell. 2019;176:198-212.e15 pubmed publisher
  7. O Grady G, Best H, Sztal T, Schartner V, Sanjuan Vazquez M, Donkervoort S, et al. Variants in the Oxidoreductase PYROXD1 Cause Early-Onset Myopathy with Internalized Nuclei and Myofibrillar Disorganization. Am J Hum Genet. 2016;99:1086-1105 pubmed publisher
  8. Tajik A, Zhang Y, Wei F, Sun J, Jia Q, Zhou W, et al. Transcription upregulation via force-induced direct stretching of chromatin. Nat Mater. 2016;15:1287-1296 pubmed publisher
  9. Eisch V, Lu X, Gabriel D, Djabali K. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget. 2016;7:24700-18 pubmed publisher
  10. Fuchs C, Gawlas S, Heher P, Nikouli S, Paar H, Ivankovic M, et al. Desmin enters the nucleus of cardiac stem cells and modulates Nkx2.5 expression by participating in transcription factor complexes that interact with the nkx2.5 gene. Biol Open. 2016;5:140-53 pubmed publisher
  11. Tapia O, Fong L, Huber M, Young S, Gerace L. Nuclear envelope protein Lem2 is required for mouse development and regulates MAP and AKT kinases. PLoS ONE. 2015;10:e0116196 pubmed publisher
  12. Gibbs Seymour I, Markiewicz E, Bekker Jensen S, Mailand N, Hutchison C. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage. Aging Cell. 2015;14:162-9 pubmed publisher
  13. Jung H, Tatar A, Tu Y, Nobumori C, Yang S, Goulbourne C, et al. An absence of nuclear lamins in keratinocytes leads to ichthyosis, defective epidermal barrier function, and intrusion of nuclear membranes and endoplasmic reticulum into the nuclear chromatin. Mol Cell Biol. 2014;34:4534-44 pubmed publisher
  14. Jafferali M, Vijayaraghavan B, Figueroa R, Crafoord E, Gudise S, Larsson V, et al. MCLIP, an effective method to detect interactions of transmembrane proteins of the nuclear envelope in live cells. Biochim Biophys Acta. 2014;1838:2399-403 pubmed publisher