This is a Validated Antibody Database (VAD) review about human dsg3, based on 28 published articles (read how Labome selects the articles), using dsg3 antibody in all methods. It is aimed to help Labome visitors find the most suited dsg3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
dsg3 synonym: CDHF6; PVA; desmoglein-3; 130 kDa pemphigus vulgaris antigen; cadherin family member 6

Invitrogen
mouse monoclonal (5G11)
  • flow cytometry; human; loading ...; fig 2c
In order to study the function of desmoglein-2 and generate knockout mice, Invitrogen dsg3 antibody (Life Technologies, 5G11) was used in flow cytometry on human samples (fig 2c). Angiogenesis (2016) ncbi
mouse monoclonal (3G133)
  • immunohistochemistry - paraffin section; human; 1:40
Invitrogen dsg3 antibody (Life technologies, 3G133) was used in immunohistochemistry - paraffin section on human samples at 1:40. J Oral Pathol Med (2015) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; mouse
  • western blot; mouse
In order to study the role of desmogleins 2 and 3 in the regulation of keratinocyte adhesion, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . J Biol Chem (2014) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; human
In order to study the role of actin-binding protein adducin in desmosomal cohesion in keratinocytes, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in immunocytochemistry on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
In order to examine desmoglein 3 expression and subcellular localization in esophageal squamous cell carcinoma, Invitrogen dsg3 antibody (Invitrogen, CLONE 5G11) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1). Acta Histochem (2014) ncbi
mouse monoclonal (5G11)
  • western blot; human
In order to investigate the role of membrane rafts during desmosome assembly and disassembly and cell adhesion, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human
In order to study the role of human eccrine sweat gland cells during epidermal regeneration, Invitrogen dsg3 antibody (Zymed, 5G11) was used in immunohistochemistry on human samples . J Invest Dermatol (2014) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; mouse
In order to study the role of DSG3 in cancer cell growth and invasion and its mechanism, Invitrogen dsg3 antibody (Zymed Laboratories Inc, 32-6300) was used in immunohistochemistry - paraffin section on mouse samples . PLoS ONE (2013) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 3
In order to identify MK2-dependent and -independent mechanisms of blister formation in a mouse model of pemphigus vulgaris, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 3). J Invest Dermatol (2014) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human; fig 1
In order to assess the use of desmoglein 3 as a predictive biomarker for lymph node metastasis in head and neck squamous cell carcinoma, Invitrogen dsg3 antibody (Invitrogen, 32-6300) was used in immunohistochemistry on human samples (fig 1). Oral Oncol (2013) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human; 1:40
In order to examine the contribution of the desmosomal compartment to gastroesophageal reflux disease, Invitrogen dsg3 antibody (Zymed, 326300) was used in immunohistochemistry on human samples at 1:40. Hum Pathol (2012) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human; 1:40; fig 1
In order to examine the role of desmosomal proteins in gastro-oesophageal reflux disease, Invitrogen dsg3 antibody (Zymed, 326300) was used in immunohistochemistry on human samples at 1:40 (fig 1). Histopathology (2012) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; human; fig 3
  • immunohistochemistry; mouse; fig 2
In order to investigate the mechanisms by which autoantibodies contribute to pemphigus vulgaris, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in immunocytochemistry on human samples (fig 3) and in immunohistochemistry on mouse samples (fig 2). J Biol Chem (2011) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; human; fig 3
  • immunohistochemistry; human; fig 1
  • western blot; human; fig 2
In order to report that desmocollin 3 is an autoantigen in pemphigus vulgaris, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in immunocytochemistry on human samples (fig 3), in immunohistochemistry on human samples (fig 1) and in western blot on human samples (fig 2). Am J Pathol (2010) ncbi
mouse monoclonal (5G11)
  • immunocytochemistry; human; 1:100
In order to investigate the role of desmosomal adhesion in the intestinal epithelial barrier function, Invitrogen dsg3 antibody (Zymed, 5G11) was used in immunocytochemistry on human samples at 1:100. Am J Physiol Gastrointest Liver Physiol (2010) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human; 1:350
In order to study the role of DNA methyltransferase-1 in maintaining progenitor function in somatic self-renewing tissue, Invitrogen dsg3 antibody (Zymed, 32-6300) was used in immunohistochemistry - paraffin section on human samples at 1:350. Nature (2010) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human; 1:25; fig 1
In order to compare infant limbus and adult limbal epithelial crypt, Invitrogen dsg3 antibody (Zymed, 32-6300) was used in immunohistochemistry on human samples at 1:25 (fig 1). Exp Eye Res (2009) ncbi
mouse monoclonal (5G11)
  • western blot; human; fig 5
In order to investigate the effect of pathogenic pemphigus vulgaris antibodies on Dsg3 during desmosome assembly, Invitrogen dsg3 antibody (Invitrogen, 5G11) was used in western blot on human samples (fig 5). J Invest Dermatol (2009) ncbi
mouse monoclonal (5G11)
  • immunoprecipitation; human; fig 2
In order to test if p120ctn associates with Dsg1 and Dsg3 in keratinocytes, Invitrogen dsg3 antibody (Zymed, 5G11) was used in immunoprecipitation on human samples (fig 2). J Dermatol (2008) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - frozen section; human
In order to investigate basement membrane components in the limbal epithelial stem cell compartment by immunohistochemistry, Invitrogen dsg3 antibody (Zymed Laboratories, 5G11) was used in immunohistochemistry - frozen section on human samples . Exp Eye Res (2007) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry; human; fig 9
In order to identify DSG3 as a therapeutic biomarker for human head and neck cancer, Invitrogen dsg3 antibody (Zymed, 32-6300) was used in immunohistochemistry on human samples (fig 9). Oncogene (2007) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human; 1:100; tbl 1
In order to develop a living skin-equivalent from de-epithelialized amnion membrane, Invitrogen dsg3 antibody (Zymed, 5G11) was used in immunohistochemistry - paraffin section on human samples at 1:100 (tbl 1). Cell Tissue Res (2006) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human; 1:30
In order to identify biomarkers for head and neck squamous cell carcinoma, Invitrogen dsg3 antibody (Zymed, 32-6300) was used in immunohistochemistry - paraffin section on human samples at 1:30. Cancer Cell (2004) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human; tbl 1
In order to discuss the role of desmosomal proteins to cancer, Invitrogen dsg3 antibody (Zymed, 5G11) was used in immunohistochemistry - paraffin section on human samples (tbl 1). J Cutan Pathol (2003) ncbi
Bio-Rad
mouse monoclonal (5G11)
  • immunocytochemistry; human; loading ...; fig s71d
  • western blot; human; 1:5000; loading ...; fig s71i
Bio-Rad dsg3 antibody (Bio-Rad, MCA2273T) was used in immunocytochemistry on human samples (fig s71d) and in western blot on human samples at 1:5000 (fig s71i). elife (2019) ncbi
mouse monoclonal (5G11)
  • immunohistochemistry - paraffin section; human
Bio-Rad dsg3 antibody (AbD Serotec, MCA2273T) was used in immunohistochemistry - paraffin section on human samples . J Cutan Pathol (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3G133)
  • western blot; scFv; fig 2
  • flow cytometry; human; fig 2
  • western blot; human; fig 2
In order to investigate the mechanisms of cell cycle regulation by the small isoform of JADE1, Santa Cruz Biotechnology dsg3 antibody (Santa Cruz Biotechnology, sc-59776) was used in western blot on scFv samples (fig 2), in flow cytometry on human samples (fig 2) and in western blot on human samples (fig 2). Cell Cycle (2015) ncbi
mouse monoclonal (5H10)
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
In order to study the role of DSG3 in cancer cell growth and invasion and its mechanism, Santa Cruz Biotechnology dsg3 antibody (Santa Cruz Biotech, 5H10) was used in immunoprecipitation on human samples , in immunocytochemistry on human samples and in western blot on human samples . PLoS ONE (2013) ncbi
Abcam
rabbit monoclonal (EPR14101)
  • immunohistochemistry; human; 1:100; fig 3
Abcam dsg3 antibody (Abcam, ab183743) was used in immunohistochemistry on human samples at 1:100 (fig 3). J Thorac Dis (2015) ncbi
Articles Reviewed
  1. Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
  2. Ebert L, Tan L, Johan M, Min K, Cockshell M, Parham K, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis. Angiogenesis. 2016;19:463-86 pubmed publisher
  3. Zhan C, Yan L, Wang L, Sun Y, Wang X, Lin Z, et al. Identification of immunohistochemical markers for distinguishing lung adenocarcinoma from squamous cell carcinoma. J Thorac Dis. 2015;7:1398-405 pubmed publisher
  4. Siriwardana N, Meyer R, Panchenko M. The novel function of JADE1S in cytokinesis of epithelial cells. Cell Cycle. 2015;14:2821-34 pubmed publisher
  5. Abé T, Maruyama S, Babkair H, Yamazaki M, Cheng J, Saku T. Simultaneous immunolocalization of desmoglein 3 and IgG4 in oral pemphigus vulgaris: IgG4 predominant autoantibodies in its pathogenesis. J Oral Pathol Med. 2015;44:850-6 pubmed publisher
  6. O Shea C, Fitzpatrick J, Koch P. Desmosomal defects in acantholytic squamous cell carcinomas. J Cutan Pathol. 2014;41:873-9 pubmed publisher
  7. Hartlieb E, Rötzer V, Radeva M, Spindler V, Waschke J. Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes. J Biol Chem. 2014;289:17043-53 pubmed publisher
  8. Rötzer V, Breit A, Waschke J, Spindler V. Adducin is required for desmosomal cohesion in keratinocytes. J Biol Chem. 2014;289:14925-40 pubmed publisher
  9. Fang W, Chen B, Xu X, Liao L, Wu Z, Wu J, et al. Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma. Acta Histochem. 2014;116:803-9 pubmed publisher
  10. Stahley S, Saito M, Faundez V, Koval M, Mattheyses A, Kowalczyk A. Desmosome assembly and disassembly are membrane raft-dependent. PLoS ONE. 2014;9:e87809 pubmed publisher
  11. Pontiggia L, Biedermann T, Böttcher Haberzeth S, Oliveira C, Braziulis E, Klar A, et al. De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells. J Invest Dermatol. 2014;134:1735-1742 pubmed publisher
  12. Chen Y, Lee L, Chao Y, Chang J, Lu Y, Li H, et al. DSG3 facilitates cancer cell growth and invasion through the DSG3-plakoglobin-TCF/LEF-Myc/cyclin D1/MMP signaling pathway. PLoS ONE. 2013;8:e64088 pubmed publisher
  13. Mao X, Li H, Sano Y, Gaestel M, Mo Park J, Payne A. MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris. J Invest Dermatol. 2014;134:68-76 pubmed publisher
  14. Patel V, Martin D, Malhotra R, Marsh C, Doçi C, Veenstra T, et al. DSG3 as a biomarker for the ultrasensitive detection of occult lymph node metastasis in oral cancer using nanostructured immunoarrays. Oral Oncol. 2013;49:93-101 pubmed publisher
  15. Wex T, Kuester D, Monkemuller K, Stahr A, Fry L, Kandulski A, et al. Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection. Hum Pathol. 2012;43:1745-54 pubmed publisher
  16. Wex T, Monkemuller K, Stahr A, Kuester D, Fry L, Völkel S, et al. Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa. Histopathology. 2012;60:405-15 pubmed publisher
  17. Mao X, Sano Y, Park J, Payne A. p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris. J Biol Chem. 2011;286:1283-91 pubmed publisher
  18. Mao X, Nagler A, Farber S, Choi E, Jackson L, Leiferman K, et al. Autoimmunity to desmocollin 3 in pemphigus vulgaris. Am J Pathol. 2010;177:2724-30 pubmed publisher
  19. Schlegel N, Meir M, Heupel W, Holthöfer B, Leube R, Waschke J. Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity. Am J Physiol Gastrointest Liver Physiol. 2010;298:G774-83 pubmed publisher
  20. Sen G, Reuter J, Webster D, Zhu L, Khavari P. DNMT1 maintains progenitor function in self-renewing somatic tissue. Nature. 2010;463:563-7 pubmed publisher
  21. Yeung A, Tint N, Kulkarni B, Mohammed I, Suleman H, Hopkinson A, et al. Infant limbus: an immunohistological study. Exp Eye Res. 2009;88:1161-4 pubmed publisher
  22. Mao X, Choi E, Payne A. Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies. J Invest Dermatol. 2009;129:908-18 pubmed publisher
  23. Kanno M, Aoyama Y, Isa Y, Yamamoto Y, Kitajima Y. P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells. J Dermatol. 2008;35:317-24 pubmed publisher
  24. Schlötzer Schrehardt U, Dietrich T, Saito K, Sorokin L, Sasaki T, Paulsson M, et al. Characterization of extracellular matrix components in the limbal epithelial stem cell compartment. Exp Eye Res. 2007;85:845-60 pubmed
  25. Chen Y, Chang J, Lee L, Wang H, Liao C, Chiu C, et al. DSG3 is overexpressed in head neck cancer and is a potential molecular target for inhibition of oncogenesis. Oncogene. 2007;26:467-76 pubmed
  26. Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, et al. New skin-equivalent model from de-epithelialized amnion membrane. Cell Tissue Res. 2006;326:69-77 pubmed
  27. Chung C, Parker J, Karaca G, Wu J, Funkhouser W, Moore D, et al. Molecular classification of head and neck squamous cell carcinomas using patterns of gene expression. Cancer Cell. 2004;5:489-500 pubmed
  28. Kurzen H, Münzing I, Hartschuh W. Expression of desmosomal proteins in squamous cell carcinomas of the skin. J Cutan Pathol. 2003;30:621-30 pubmed