This is a Validated Antibody Database (VAD) review about human coilin, based on 15 published articles (read how Labome selects the articles), using coilin antibody in all methods. It is aimed to help Labome visitors find the most suited coilin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
coilin synonym: CLN80; p80-coilin; coilin; coilin p80; p80

Knockout validation
Abcam
mouse monoclonal (IH10)
  • immunocytochemistry knockout validation; human; 1:250; fig 4
  • western blot knockout validation; human; 1:250; fig 4
Abcam coilin antibody (Abcam, IH10) was used in immunocytochemistry knockout validation on human samples at 1:250 (fig 4) and in western blot knockout validation on human samples at 1:250 (fig 4). elife (2016) ncbi
Abcam
mouse monoclonal (IH10)
  • immunocytochemistry; human; 1:400; loading ...; fig s5e
Abcam coilin antibody (Abcam, ab87913) was used in immunocytochemistry on human samples at 1:400 (fig s5e). Nature (2018) ncbi
mouse monoclonal (IH10)
  • immunocytochemistry knockout validation; human; 1:250; fig 4
  • western blot knockout validation; human; 1:250; fig 4
Abcam coilin antibody (Abcam, IH10) was used in immunocytochemistry knockout validation on human samples at 1:250 (fig 4) and in western blot knockout validation on human samples at 1:250 (fig 4). elife (2016) ncbi
mouse monoclonal (IH10)
  • immunocytochemistry; human; loading ...; fig 5e
Abcam coilin antibody (Abcam, ab87913) was used in immunocytochemistry on human samples (fig 5e). Nucleic Acids Res (2016) ncbi
mouse monoclonal (IH10)
  • immunocytochemistry; mouse; fig 3
Abcam coilin antibody (Abcam, ab87913) was used in immunocytochemistry on mouse samples (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (IH10)
  • immunocytochemistry; human
Abcam coilin antibody (Abcam, ab87913) was used in immunocytochemistry on human samples . Mol Cell Biol (2014) ncbi
mouse monoclonal (IH10)
  • immunocytochemistry; human; 1:200
Abcam coilin antibody (Abcam, Ab87913) was used in immunocytochemistry on human samples at 1:200. PLoS ONE (2013) ncbi
mouse monoclonal (IH10)
  • western blot; human
Abcam coilin antibody (Abcam, ab87913) was used in western blot on human samples . FEBS Lett (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (Pdelta)
  • immunoprecipitation; human; 1:200; fig 7
  • immunocytochemistry; human; 1:50; fig 2
In order to elucidate how VRK1 regulates Cajal bodies stability and dynamics, Santa Cruz Biotechnology coilin antibody (Santa Cruz, sc-56298) was used in immunoprecipitation on human samples at 1:200 (fig 7) and in immunocytochemistry on human samples at 1:50 (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (F-7)
  • other; human; fig 2
In order to characterize capabilities and potentials of extracellular vesicle (EV) array, Santa Cruz Biotechnology coilin antibody (Santa Cruz Biotechnology, sc-55594) was used in other on human samples (fig 2). J Extracell Vesicles (2015) ncbi
mouse monoclonal (F-7)
  • immunocytochemistry; human; 1:1000; loading ...; fig S1A
Santa Cruz Biotechnology coilin antibody (Santa Cruz, F-7) was used in immunocytochemistry on human samples at 1:1000 (fig S1A). Nucleic Acids Res (2015) ncbi
mouse monoclonal (Pdelta)
  • western blot; human; loading ...; fig 2e
Santa Cruz Biotechnology coilin antibody (Santa Cruz, sc-56298) was used in western blot on human samples (fig 2e). Sci Rep (2014) ncbi
BD Biosciences
mouse monoclonal (56/Coilin)
  • immunocytochemistry; human
In order to investigate the cellular localization and function of negative transcription elongation factor , BD Biosciences coilin antibody (BD Biosciences, 612074) was used in immunocytochemistry on human samples . Exp Cell Res (2009) ncbi
mouse monoclonal (56/Coilin)
  • immunocytochemistry; human; loading ...; fig 5
In order to examine the contribution of Gemin8 to the composition and function of the survival motor neuron protein complex, BD Biosciences coilin antibody (BD, 56) was used in immunocytochemistry on human samples (fig 5). J Biol Chem (2006) ncbi
Sigma-Aldrich
mouse monoclonal (Pdelta)
  • immunocytochemistry; human; fig 1
Sigma-Aldrich coilin antibody (Sigma, C-1862) was used in immunocytochemistry on human samples (fig 1). Nucleic Acids Res (2015) ncbi
mouse monoclonal (Pdelta)
  • immunohistochemistry - paraffin section; human; fig 1
In order to study the role of loss of spliceosome integrity in the development of amyotrophic lateral sclerosis and spinal muscular atrophy, Sigma-Aldrich coilin antibody (Sigma, C1862) was used in immunohistochemistry - paraffin section on human samples (fig 1). EMBO Mol Med (2013) ncbi
Articles Reviewed
  1. Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed publisher
  2. Vogan J, Zhang X, Youmans D, Regalado S, Johnson J, Hockemeyer D, et al. Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies. elife. 2016;5: pubmed publisher
  3. Zhang Y, Wu Y, Mao P, Li F, Han X, Zhang Y, et al. Cold-inducible RNA-binding protein CIRP/hnRNP A18 regulates telomerase activity in a temperature-dependent manner. Nucleic Acids Res. 2016;44:761-75 pubmed publisher
  4. Golov A, Gavrilov A, Razin S. The Role of Crowding Forces in Juxtaposing β-Globin Gene Domain Remote Regulatory Elements in Mouse Erythroid Cells. PLoS ONE. 2015;10:e0139855 pubmed publisher
  5. Cantarero L, Sanz García M, Vinograd Byk H, Renbaum P, Levy Lahad E, Lazo P. VRK1 regulates Cajal body dynamics and protects coilin from proteasomal degradation in cell cycle. Sci Rep. 2015;5:10543 pubmed publisher
  6. Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
  7. Yu Y, Chi B, Xia W, Gangopadhyay J, Yamazaki T, Winkelbauer Hurt M, et al. U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish. Nucleic Acids Res. 2015;43:3208-18 pubmed publisher
  8. Schertzer M, Jouravleva K, Perderiset M, Dingli F, Loew D, Le Guen T, et al. Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Res. 2015;43:1834-47 pubmed publisher
  9. Shaiken T, Opekun A. Dissecting the cell to nucleus, perinucleus and cytosol. Sci Rep. 2014;4:4923 pubmed publisher
  10. Maida Y, Yasukawa M, Okamoto N, Ohka S, Kinoshita K, Totoki Y, et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 2014;34:1576-93 pubmed publisher
  11. Morchikh M, Naughtin M, Di Nunzio F, Xavier J, Charneau P, Jacob Y, et al. TOX4 and NOVA1 proteins are partners of the LEDGF PWWP domain and affect HIV-1 replication. PLoS ONE. 2013;8:e81217 pubmed publisher
  12. Das S, Cong R, Shandilya J, Senapati P, Moindrot B, Monier K, et al. Characterization of nucleolin K88 acetylation defines a new pool of nucleolin colocalizing with pre-mRNA splicing factors. FEBS Lett. 2013;587:417-24 pubmed publisher
  13. Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
  14. Yung T, Narita T, Komori T, Yamaguchi Y, Handa H. Cellular dynamics of the negative transcription elongation factor NELF. Exp Cell Res. 2009;315:1693-705 pubmed publisher
  15. Carissimi C, Saieva L, Gabanella F, Pellizzoni L. Gemin8 is required for the architecture and function of the survival motor neuron complex. J Biol Chem. 2006;281:37009-16 pubmed