This is a Validated Antibody Database (VAD) review about human calsequestrin, based on 33 published articles (read how Labome selects the articles), using calsequestrin antibody in all methods. It is aimed to help Labome visitors find the most suited calsequestrin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
calsequestrin synonym: CASQ; PDIB1; VMCQA; calsequestrin-1; calmitin; calmitine; calsequestrin 1 (fast-twitch, skeletal muscle); calsequestrin, skeletal muscle isoform

Invitrogen
mouse monoclonal (VIIID12)
  • western blot; mouse; loading ...; fig 5a
In order to report the effects of short- and long-term high-fat diets on fast-twitch skeletal muscles, Invitrogen calsequestrin antibody (Thermo Scientific, MA3-913) was used in western blot on mouse samples (fig 5a). Physiol Rep (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:400; fig 3c
  • western blot; mouse; 1:1000; loading ...; fig s2f
In order to elucidate the mechanism by which the I4895T mutation in the type 1 ryanodine receptor/Ca(2+) release channel results in disease, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in immunohistochemistry on mouse samples at 1:400 (fig 3c) and in western blot on mouse samples at 1:1000 (fig s2f). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:2500; loading ...; fig 1b
In order to determine the role of calcium regulation in sporadic inclusion body myositis, Invitrogen calsequestrin antibody (Thermo Fischer, PA1-913) was used in western blot on human samples at 1:2500 (fig 1b). Acta Neuropathol Commun (2017) ncbi
mouse monoclonal (VIIID12)
  • western blot; mouse; loading ...; fig 2f
In order to report the physiological role of sarcolipin upregulation in muscle myopathy, Invitrogen calsequestrin antibody (Pierce Antibodies, MA3-913) was used in western blot on mouse samples (fig 2f). PLoS ONE (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 4a
In order to test if the TRPM4 channel contributes to physiological cardiac hypertrophy, Invitrogen calsequestrin antibody (Fisher Scientific, PA1-913) was used in western blot on mouse samples (fig 4a). J Muscle Res Cell Motil (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 10
In order to study mouse models of dilated cardiomyopathy to study calcium signaling, Invitrogen calsequestrin antibody (Affinity BioReagents, pA-913) was used in western blot on mouse samples (fig 10). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5a
In order to examine the effects of sildenafil on skeletal muscle, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on mouse samples at 1:1000 (fig 5a). Exp Mol Med (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
In order to discuss how mutations in sarcomere genes may result in altered calcium signaling and heart defects, Invitrogen calsequestrin antibody (Thermo Pierce, PA1-913) was used in western blot on human samples at 1:1000 (fig 1a). Circulation (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 1b
In order to examine the effects of marijuana on CB1R activation in skeletal muscle, Invitrogen calsequestrin antibody (Thermo Fisher, PA1-913) was used in western blot on mouse samples at 1:500 (fig 1b). J Physiol (2016) ncbi
mouse monoclonal (VIIID12)
  • western blot; mouse; 1:2000; fig 3
In order to study affects of acute minimization of brown adipose tissue function by increased reliance on muscle based thermogenesis, Invitrogen calsequestrin antibody (Thermo Fisher Scientific, MA3-913) was used in western blot on mouse samples at 1:2000 (fig 3). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to study prevention of phospholemman (PLM) inhibition of the sodium-calcium exchanger 1 (NCX1) via development of high affinity peptides, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on human samples (fig 1). Biochem J (2016) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 7a
In order to study the role of angiotensin receptor-associated protein Atrap on cardiac Ca2+-ATPase SERCA2a, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on mouse samples (fig 7a). Cardiovasc Res (2016) ncbi
rabbit polyclonal
  • western blot; rat; fig 11
In order to research how protein phosphatase 1c associates with cardiac sodium calcium exchanger 1, Invitrogen calsequestrin antibody (Thermo Fisher, PA1-913) was used in western blot on rat samples (fig 11). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3
In order to maintain cardiac function through a heterozygous deletion of sarcolipin, Invitrogen calsequestrin antibody (Affinity BioReagents, PA1-913) was used in western blot on mouse samples (fig 3). Am J Physiol Heart Circ Physiol (2016) ncbi
mouse monoclonal (VIIID12)
  • western blot; rat; 1:3000; fig 3
In order to study the effects of caloric restriction skeletal muscle contraction, thyroid hormone metabolism, and fiber composition upon refeeding, Invitrogen calsequestrin antibody (Thermo Fisher Scientific, MA3-913) was used in western blot on rat samples at 1:3000 (fig 3). Front Physiol (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000; fig 3
In order to study the effects of caloric restriction skeletal muscle contraction, thyroid hormone metabolism, and fiber composition upon refeeding, Invitrogen calsequestrin antibody (Thermo Fisher Scientific, PA1-913) was used in western blot on rat samples at 1:2000 (fig 3). Front Physiol (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:2500; fig 2
In order to determine Sox9 expression in eleven different types of canine skin neoplasms, Invitrogen calsequestrin antibody (Fisher Scientific, PA1-913) was used in western blot on rat samples at 1:2500 (fig 2). Eur J Histochem (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:2500; fig 2
In order to test if p11 regulates the serotonin receptor 4 pathway in the heart, Invitrogen calsequestrin antibody (Fisher Scientific, PA1-913) was used in western blot on rat samples at 1:2500 (fig 2). Cell Calcium (2015) ncbi
rabbit polyclonal
  • western blot; rat
In order to examine calcium-related proteins in the intrinsic laryngeal muscles, cricothyroid, and tibialis anterior muscles from male Sprague-Dawley rats, Invitrogen calsequestrin antibody (Affinity Bioreagents, PA1-913) was used in western blot on rat samples . Physiol Rep (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4
In order to compare the transduction efficiency of recombinant adeno-associated vectors, adenoviruses, and lentiviral vectors in human embryonic stem cells, human induced pluripotent stem cells, and the derived cardiomyocytes, Invitrogen calsequestrin antibody (ThermoScientific, PA1-913) was used in western blot on human samples at 1:1000 (fig 4). Mol Ther Methods Clin Dev (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 1c
In order to study the role of SERCA1b during skeletal muscle differentiation, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on mouse samples at 1:1000 (fig 1c). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to investigate Mitsugumin 56 in skeletal muscle maturation using knockout mice, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on mouse samples . FEBS Lett (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to determine the effect of perhexiline treatment on the cardiac metabolome in wild type and Mybpc3-targeted knock-in mice, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on mouse samples . Mol Biosyst (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study the metabolic responses during chronic pressure overloads in mouse hearts expressing ssTnI, Invitrogen calsequestrin antibody (Pierce Thermo Scientific, PA1-913) was used in western blot on mouse samples . Circ Heart Fail (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
  • western blot; mouse; 1:1000
In order to study the loss of thd miR-106b-25 cluster in the pathogenesis of atrial fibrillation, Invitrogen calsequestrin antibody (Thermo Fisher Scientific, PA1-913) was used in western blot on human samples at 1:1000 and in western blot on mouse samples at 1:1000. Circ Arrhythm Electrophysiol (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to determine the relationship between calpain and NCX1 in the heart, Invitrogen calsequestrin antibody (Thermo Scientific, PA1-913) was used in western blot on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (VIIID12)
  • immunohistochemistry; mouse; 1:300
In order to study a missense mutation in the calsequestrin-1 gene discovered in a group of patients with a myopathy, Invitrogen calsequestrin antibody (Thermo Scientific, MA3-913) was used in immunohistochemistry on mouse samples at 1:300. Hum Mutat (2014) ncbi
mouse monoclonal (VIIID12)
  • western blot; human
In order to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure, Invitrogen calsequestrin antibody (Pierce, VIIID12) was used in western blot on human samples . J Card Fail (2012) ncbi
mouse monoclonal (VIIID12)
  • western blot; rabbit; 1:2500; fig 8
In order to characterize the interaction between junctophilin 1 and the RyR1 channel complex, Invitrogen calsequestrin antibody (Affinity BioReagents, MA3-913) was used in western blot on rabbit samples at 1:2500 (fig 8). J Biol Chem (2007) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-10)
  • immunoprecipitation; human; 1:1000; loading ...; fig 1b
Santa Cruz Biotechnology calsequestrin antibody (Santa Cruz Biotechnology, SC-137080) was used in immunoprecipitation on human samples at 1:1000 (fig 1b). Sci Rep (2015) ncbi
mouse monoclonal (D-10)
  • western blot; pig
Santa Cruz Biotechnology calsequestrin antibody (Santa Cruz Biotechnologies, D-10) was used in western blot on pig samples . Int J Legal Med (2016) ncbi
MilliporeSigma
rabbit polyclonal
  • western blot; mouse; 1:3000; fig 2
In order to analyze slow twitch skeletal muscles and the role of the JP45-calsequestrin complex on calcium entry, MilliporeSigma calsequestrin antibody (Sigma, C0743) was used in western blot on mouse samples at 1:3000 (fig 2). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to assess the function and characterization of extraocular muscles and obicularis oculi, MilliporeSigma calsequestrin antibody (Sigma, C-0743) was used in western blot on human samples (fig 1). J Gen Physiol (2016) ncbi
rabbit polyclonal
  • western blot; human
In order to study the role of RyR in regulating calcium levels in extraocular muscles, MilliporeSigma calsequestrin antibody (Sigma, C-0743) was used in western blot on human samples . Biochem J (2015) ncbi
Articles Reviewed
  1. Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
  2. Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
  3. Amici D, Pinal Fernández I, Mázala D, Lloyd T, Corse A, Christopher Stine L, et al. Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis. Acta Neuropathol Commun. 2017;5:24 pubmed publisher
  4. Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  5. Gueffier M, Zintz J, Lambert K, Finan A, Aimond F, Chakouri N, et al. The TRPM4 channel is functionally important for the beneficial cardiac remodeling induced by endurance training. J Muscle Res Cell Motil. 2017;38:3-16 pubmed publisher
  6. Ramratnam M, Salama G, Sharma R, Wang D, Smith S, Banerjee S, et al. Gene-Targeted Mice with the Human Troponin T R141W Mutation Develop Dilated Cardiomyopathy with Calcium Desensitization. PLoS ONE. 2016;11:e0167681 pubmed publisher
  7. Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
  8. Helms A, Alvarado F, Yob J, Tang V, Pagani F, Russell M, et al. Genotype-Dependent and -Independent Calcium Signaling Dysregulation in Human Hypertrophic Cardiomyopathy. Circulation. 2016;134:1738-1748 pubmed
  9. Oláh T, Bodnár D, Tóth A, Vincze J, Fodor J, Reischl B, et al. Cannabinoid signalling inhibits sarcoplasmic Ca2+ release and regulates excitation-contraction coupling in mammalian skeletal muscle. J Physiol. 2016;594:7381-7398 pubmed publisher
  10. Bal N, Maurya S, Singh S, Wehrens X, Periasamy M. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem. 2016;291:17247-57 pubmed publisher
  11. Wanichawan P, Hodne K, Hafver T, Lunde M, Martinsen M, Louch W, et al. Development of a high-affinity peptide that prevents phospholemman (PLM) inhibition of the sodium/calcium exchanger 1 (NCX1). Biochem J. 2016;473:2413-23 pubmed publisher
  12. Mosca B, Eckhardt J, Bergamelli L, Treves S, Bongianino R, De Negri M, et al. Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles. J Biol Chem. 2016;291:14555-65 pubmed publisher
  13. Sekulic Jablanovic M, Ullrich N, Goldblum D, Palmowski Wolfe A, Zorzato F, Treves S. Functional characterization of orbicularis oculi and extraocular muscles. J Gen Physiol. 2016;147:395-406 pubmed publisher
  14. Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach K, Grill A, et al. The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a. Cardiovasc Res. 2016;110:359-70 pubmed publisher
  15. Hafver T, Hodne K, Wanichawan P, Aronsen J, Dalhus B, Lunde P, et al. Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman. J Biol Chem. 2016;291:4561-79 pubmed publisher
  16. Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, et al. Heterozygous deletion of sarcolipin maintains normal cardiac function. Am J Physiol Heart Circ Physiol. 2016;310:H92-103 pubmed publisher
  17. de Andrade P, Neff L, Strosova M, Arsenijevic D, Patthey Vuadens O, Scapozza L, et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol. 2015;6:254 pubmed publisher
  18. Fantinato E, Milani L, Sironi G. Sox9 expression in canine epithelial skin tumors. Eur J Histochem. 2015;59:2514 pubmed publisher
  19. Meschin P, Demion M, Cazorla O, Finan A, Thireau J, Richard S, et al. p11 modulates calcium handling through 5-HTâ‚„R pathway in rat ventricular cardiomyocytes. Cell Calcium. 2015;58:549-57 pubmed publisher
  20. Ferretti R, Marques M, Khurana T, Santo Neto H. Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles. Physiol Rep. 2015;3: pubmed publisher
  21. Wang L, Zhang L, Li S, Zheng Y, Yan X, Chen M, et al. Retrograde regulation of STIM1-Orai1 interaction and store-operated Ca2+ entry by calsequestrin. Sci Rep. 2015;5:11349 pubmed publisher
  22. Rapti K, Stillitano F, Karakikes I, Nonnenmacher M, Weber T, Hulot J, et al. Effectiveness of gene delivery systems for pluripotent and differentiated cells. Mol Ther Methods Clin Dev. 2015;2:14067 pubmed publisher
  23. Foditsch E, Saenger A, Monticelli F. Skeletal muscle proteins: a new approach to delimitate the time since death. Int J Legal Med. 2016;130:433-40 pubmed publisher
  24. Tóth A, Fodor J, Vincze J, Oláh T, Juhász T, Zákány R, et al. The Effect of SERCA1b Silencing on the Differentiation and Calcium Homeostasis of C2C12 Skeletal Muscle Cells. PLoS ONE. 2015;10:e0123583 pubmed publisher
  25. Van B, Nishi M, Komazaki S, Ichimura A, Kakizawa S, Nakanaga K, et al. Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation. FEBS Lett. 2015;589:1095-104 pubmed publisher
  26. Gehmlich K, Dodd M, Allwood J, Kelly M, Bellahcene M, Lad H, et al. Changes in the cardiac metabolome caused by perhexiline treatment in a mouse model of hypertrophic cardiomyopathy. Mol Biosyst. 2015;11:564-73 pubmed publisher
  27. Carley A, Taglieri D, Bi J, Solaro R, Lewandowski E. Metabolic efficiency promotes protection from pressure overload in hearts expressing slow skeletal troponin I. Circ Heart Fail. 2015;8:119-27 pubmed publisher
  28. Chiang D, Kongchan N, Beavers D, Alsina K, Voigt N, Neilson J, et al. Loss of microRNA-106b-25 cluster promotes atrial fibrillation by enhancing ryanodine receptor type-2 expression and calcium release. Circ Arrhythm Electrophysiol. 2014;7:1214-22 pubmed publisher
  29. Sekulic Jablanovic M, Palmowski Wolfe A, Zorzato F, Treves S. Characterization of excitation-contraction coupling components in human extraocular muscles. Biochem J. 2015;466:29-36 pubmed publisher
  30. Wanichawan P, Hafver T, Hodne K, Aronsen J, Lunde I, Dalhus B, et al. Molecular basis of calpain cleavage and inactivation of the sodium-calcium exchanger 1 in heart failure. J Biol Chem. 2014;289:33984-98 pubmed publisher
  31. Rossi D, Vezzani B, Galli L, Paolini C, Toniolo L, Pierantozzi E, et al. A mutation in the CASQ1 gene causes a vacuolar myopathy with accumulation of sarcoplasmic reticulum protein aggregates. Hum Mutat. 2014;35:1163-70 pubmed publisher
  32. Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher
  33. Phimister A, Lango J, Lee E, Ernst Russell M, Takeshima H, Ma J, et al. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem. 2007;282:8667-77 pubmed