This is a Validated Antibody Database (VAD) review about human aquaporin 4, based on 20 published articles (read how Labome selects the articles), using aquaporin 4 antibody in all methods. It is aimed to help Labome visitors find the most suited aquaporin 4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
aquaporin 4 synonym: MIWC; WCH4

Knockout validation
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1e
MilliporeSigma aquaporin 4 antibody (MilliporeSigma, A5971) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1e). J Clin Invest (2020) ncbi
Abcam
mouse monoclonal (43191)
  • immunohistochemistry - frozen section; domestic rabbit; loading ...; fig 5a
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in immunohistochemistry - frozen section on domestic rabbit samples (fig 5a). Front Cell Neurosci (2021) ncbi
mouse monoclonal (43191)
  • immunohistochemistry - paraffin section; mouse; 1:60; loading ...; fig 5di
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in immunohistochemistry - paraffin section on mouse samples at 1:60 (fig 5di). Brain (2020) ncbi
mouse monoclonal (43191)
  • ELISA; rat; 1:500; loading ...
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in ELISA on rat samples at 1:500. Cell (2020) ncbi
domestic rabbit monoclonal (EPR7040)
  • immunohistochemistry - frozen section; rat; 1:400; loading ...; fig 2c
  • ELISA; human; 1:500; loading ...; fig 1
Abcam aquaporin 4 antibody (Abcam, ab128906) was used in immunohistochemistry - frozen section on rat samples at 1:400 (fig 2c) and in ELISA on human samples at 1:500 (fig 1). Cell (2020) ncbi
domestic rabbit monoclonal (EPR7040)
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 2a
Abcam aquaporin 4 antibody (Abcam, ab128906) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 2a). Exp Ther Med (2020) ncbi
domestic rabbit monoclonal (EPR7040)
  • ELISA; human; fig 4
Abcam aquaporin 4 antibody (Abcam, ab128906) was used in ELISA on human samples (fig 4). Eur J Neurosci (2017) ncbi
mouse monoclonal (43191)
  • ELISA; human; fig 3
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in ELISA on human samples (fig 3). Eur J Neurosci (2017) ncbi
mouse monoclonal (43191)
  • immunohistochemistry; rat; 1:100; loading ...; fig 5g
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in immunohistochemistry on rat samples at 1:100 (fig 5g). PLoS ONE (2017) ncbi
mouse monoclonal (43191)
  • immunocytochemistry; rat; fig 5d
  • immunohistochemistry; rat; 1:100; fig 2a
  • western blot; rat; 1:500; fig 6a
Abcam aquaporin 4 antibody (Abcam, ab9512) was used in immunocytochemistry on rat samples (fig 5d), in immunohistochemistry on rat samples at 1:100 (fig 2a) and in western blot on rat samples at 1:500 (fig 6a). J Inflamm (Lond) (2017) ncbi
mouse monoclonal (43191)
  • immunohistochemistry - paraffin section; rat; 1:100
In order to determine the expression and role of aquaporins in spinal cord, Abcam aquaporin 4 antibody (Abcam, ab9512) was used in immunohistochemistry - paraffin section on rat samples at 1:100. J Histochem Cytochem (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (4/18)
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 2o
Santa Cruz Biotechnology aquaporin 4 antibody (Santa Cruz, sc-32739) was used in immunohistochemistry - frozen section on mouse samples at 1:250 (fig 2o). elife (2019) ncbi
Alomone Labs
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:200
In order to assess using recombinant adeno-associated virus rhesus-10 as a gene therapy vector in the central nervous system, Alomone Labs aquaporin 4 antibody (Alomone lab, AQP-004) was used in immunohistochemistry - free floating section on mouse samples at 1:200. Mol Neurobiol (2016) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 7a, 7b, 7c, 7d
Novus Biologicals aquaporin 4 antibody (Novus, NBP1?C87679) was used in immunohistochemistry on rat samples at 1:500 (fig 7a, 7b, 7c, 7d). Sci Rep (2020) ncbi
StressMarq Biosciences
domestic rabbit polyclonal
  • western blot; mouse; fig 1
StressMarq Biosciences aquaporin 4 antibody (StressMarq, SPC-505D) was used in western blot on mouse samples (fig 1). PLoS ONE (2016) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 1b
MilliporeSigma aquaporin 4 antibody (Sigma, SAB5200112) was used in immunohistochemistry - frozen section on rat samples at 1:50 (fig 1b). J Neuroinflammation (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1e
MilliporeSigma aquaporin 4 antibody (MilliporeSigma, A5971) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1e). J Clin Invest (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 1c
MilliporeSigma aquaporin 4 antibody (Sigma-Aldrich, A5971) was used in immunohistochemistry on mouse samples (fig 2a) and in western blot on mouse samples at 1:1000 (fig 1c). Mol Brain (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 3c
MilliporeSigma aquaporin 4 antibody (Sigma Aldrich, A5971) was used in immunohistochemistry - frozen section on mouse samples at 1:400 (fig 3c). Brain Struct Funct (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2d
MilliporeSigma aquaporin 4 antibody (Sigma, HPA014784) was used in immunohistochemistry on mouse samples at 1:200 (fig 2d). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; fig 2c
MilliporeSigma aquaporin 4 antibody (Sigma-Aldrich, A5971) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2c). J Neuroimmunol (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig s5a
In order to identify an astrocyte subtype induced by activated microglia, MilliporeSigma aquaporin 4 antibody (Sigma, HPA014784) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig s5a). Nature (2017) ncbi
domestic rabbit polyclonal
  • other; mouse; 1:400; loading ...; fig 2a
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 3a
  • western blot; mouse; 1:5000; loading ...; fig 5c
In order to elucidate how alpha-syntrophin governs aquaporin-4 levels in the brain, MilliporeSigma aquaporin 4 antibody (Sigma, A5971) was used in other on mouse samples at 1:400 (fig 2a), in immunohistochemistry - frozen section on mouse samples at 1:400 (fig 3a) and in western blot on mouse samples at 1:5000 (fig 5c). Brain Struct Funct (2017) ncbi
Articles Reviewed
  1. Ishikura T, Kinoshita M, Shimizu M, Yasumizu Y, Motooka D, Okuzaki D, et al. Anti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats. J Neuroinflammation. 2021;18:181 pubmed publisher
  2. Purohit D, Finkel D, Malfa A, Liao Y, Ivanova L, Kleinman G, et al. Human Cord Blood Derived Unrestricted Somatic Stem Cells Restore Aquaporin Channel Expression, Reduce Inflammation and Inhibit the Development of Hydrocephalus After Experimentally Induced Perinatal Intraventricular Hemorrhage. Front Cell Neurosci. 2021;15:633185 pubmed publisher
  3. Harrison I, Ismail O, Machhada A, Colgan N, Ohene Y, Nahavandi P, et al. Impaired glymphatic function and clearance of tau in an Alzheimer's disease model. Brain. 2020;143:2576-2593 pubmed publisher
  4. Chen T, Lennon V, Liu Y, Bosco D, Li Y, Yi M, et al. Astrocyte-microglia interaction drives evolving neuromyelitis optica lesion. J Clin Invest. 2020;130:4025-4038 pubmed publisher
  5. Kitchen P, Salman M, Halsey A, Clarke Bland C, MacDonald J, Ishida H, et al. Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell. 2020;181:784-799.e19 pubmed publisher
  6. Katoozi S, Rao S, Skauli N, Froehner S, Ottersen O, Adams M, et al. Functional specialization of retinal Müller cell endfeet depends on an interplay between two syntrophin isoforms. Mol Brain. 2020;13:40 pubmed publisher
  7. Guan Y, Li L, Chen J, Lu H. Effect of AQP4-RNAi in treating traumatic brain edema: Multi-modal MRI and histopathological changes of early stage edema in a rat model. Exp Ther Med. 2020;19:2029-2036 pubmed publisher
  8. Hoddevik E, Rao S, Zahl S, Boldt H, Ottersen O, Amiry Moghaddam M. Organisation of extracellular matrix proteins laminin and agrin in pericapillary basal laminae in mouse brain. Brain Struct Funct. 2020;225:805-816 pubmed publisher
  9. Koundal S, Elkin R, Nadeem S, Xue Y, Constantinou S, Sanggaard S, et al. Optimal Mass Transport with Lagrangian Workflow Reveals Advective and Diffusion Driven Solute Transport in the Glymphatic System. Sci Rep. 2020;10:1990 pubmed publisher
  10. Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  11. Norwood J, Zhang Q, CARD D, Craine A, Ryan T, Drew P. Anatomical basis and physiological role of cerebrospinal fluid transport through the murine cribriform plate. elife. 2019;8: pubmed publisher
  12. Salman M, Kitchen P, Woodroofe M, Brown J, Bill R, Conner A, et al. Hypothermia increases aquaporin 4 (AQP4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV4)- and calmodulin-mediated mechanism. Eur J Neurosci. 2017;46:2542-2547 pubmed publisher
  13. Kon T, Ueno T, Suzuki C, Nunomura J, Igarashi S, Sato T, et al. Aquaporin-4 antibody positive neuromyelitis optica spectrum disorder associated with esophageal cancer. J Neuroimmunol. 2017;309:38-40 pubmed publisher
  14. Puhakka N, Bot A, Vuokila N, Dębski K, Lukasiuk K, Pitkanen A. Chronically dysregulated NOTCH1 interactome in the dentate gyrus after traumatic brain injury. PLoS ONE. 2017;12:e0172521 pubmed publisher
  15. Cai L, Lei C, Li R, Chen W, Hu C, Chen X, et al. Overexpression of aquaporin 4 in articular chondrocytes exacerbates the severity of adjuvant-induced arthritis in rats: an in vivo and in vitro study. J Inflamm (Lond). 2017;14:6 pubmed publisher
  16. Liddelow S, Guttenplan K, Clarke L, Bennett F, Bohlen C, Schirmer L, et al. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017;541:481-487 pubmed publisher
  17. Hoddevik E, Khan F, Rahmani S, Ottersen O, Boldt H, Amiry Moghaddam M. Factors determining the density of AQP4 water channel molecules at the brain-blood interface. Brain Struct Funct. 2017;222:1753-1766 pubmed publisher
  18. Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed publisher
  19. Guo Y, Wang D, Qiao T, Yang C, Su Q, Gao G, et al. A Single Injection of Recombinant Adeno-Associated Virus into the Lumbar Cistern Delivers Transgene Expression Throughout the Whole Spinal Cord. Mol Neurobiol. 2016;53:3235-3248 pubmed publisher
  20. Oklinski M, Lim J, Choi H, Oklinska P, Skowronski M, Kwon T. Immunolocalization of Water Channel Proteins AQP1 and AQP4 in Rat Spinal Cord. J Histochem Cytochem. 2014;62:598-611 pubmed publisher