This is a Validated Antibody Database (VAD) review about human annexin II, based on 32 published articles (read how Labome selects the articles), using annexin II antibody in all methods. It is aimed to help Labome visitors find the most suited annexin II antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
annexin II synonym: ANX2; ANX2L4; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV; annexin A2; annexin II; annexin-2; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; chromobindin 8; epididymis secretory protein Li 270; lipocortin II; placental anticoagulant protein IV; protein I

Knockout validation
BD Biosciences
mouse monoclonal (5/Annexin II)
  • immunocytochemistry knockout validation; mouse; fig 1
  • immunohistochemistry knockout validation; mouse; fig 1
BD Biosciences annexin II antibody (BD Pharmingen), 610069) was used in immunocytochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 1). Mol Cancer Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-10)
  • western blot; mouse; loading ...; fig 2i
Santa Cruz Biotechnology annexin II antibody (Santa Cruz Biotechnology Inc, sc-28385) was used in western blot on mouse samples (fig 2i). Cancer Res (2018) ncbi
mouse monoclonal (85.Tyr 24)
  • immunocytochemistry; rat; 1:20; loading ...; fig 3
In order to investigate the role of Ser25 phosphorylation in subcellular localization of Annexin A2 and its interaction with mRNP complexes, Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-135753) was used in immunocytochemistry on rat samples at 1:20 (fig 3). FEBS Open Bio (2017) ncbi
mouse monoclonal (H-5)
  • immunocytochemistry; human; fig 1a
  • western blot; human; loading ...; fig 3c
In order to present the role of CD147 in cancer migration through annexin A2 and DOCK3-catenin-WAVE2 signaling, Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-48397) was used in immunocytochemistry on human samples (fig 1a) and in western blot on human samples (fig 3c). Oncotarget (2016) ncbi
mouse monoclonal (85.Tyr 24)
  • western blot; human; loading ...; fig 2a
In order to present the role of CD147 in cancer migration through annexin A2 and DOCK3-catenin-WAVE2 signaling, Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-135753) was used in western blot on human samples (fig 2a). Oncotarget (2016) ncbi
mouse monoclonal (85.Tyr 24)
  • immunocytochemistry; rat; 1:20; fig 4
  • western blot; rat; 1:200; fig 5
In order to study how Tyr23 phosphorylation regulates AnxA2 function, Santa Cruz Biotechnology annexin II antibody (Santa Cruz Biotechnologies, sc-135753) was used in immunocytochemistry on rat samples at 1:20 (fig 4) and in western blot on rat samples at 1:200 (fig 5). J Cell Sci (2016) ncbi
mouse monoclonal (C-10)
  • western blot; human; 1:1000; fig 1
Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-28385) was used in western blot on human samples at 1:1000 (fig 1). Mol Psychiatry (2015) ncbi
mouse monoclonal (3D5)
  • chromatin immunoprecipitation; human; 1:100; fig 2
In order to determine how starvation-induced autophagy is promoted by transcriptional regulation of Annexin A2, Santa Cruz Biotechnology annexin II antibody (Santa Cruz, 3D5) was used in chromatin immunoprecipitation on human samples at 1:100 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (11.Tyr 24)
  • western blot; human
Santa Cruz Biotechnology annexin II antibody (Santa Cruz Biotechnology, sc-135752) was used in western blot on human samples . Transl Res (2015) ncbi
mouse monoclonal (C-10)
  • western blot; human; loading ...; fig 3d
In order to perform a proteomic analysis of HBV-infected HepG2 cells to identify changes in proteins involved in cell migration, Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-28385) was used in western blot on human samples (fig 3d). PLoS ONE (2014) ncbi
mouse monoclonal (3D5)
Santa Cruz Biotechnology annexin II antibody (Santa Cruz, sc-47696) was used . Obesity (Silver Spring) (2014) ncbi
Invitrogen
goat polyclonal
  • western blot; mouse; 1:1000; fig 2
In order to determine inhibition of NLRC4-mediated inflammasome activation and binding to annexin A2 by the tick protein sialostatin L2, Invitrogen annexin II antibody (Thermo Scientific, PA1-9006) was used in western blot on mouse samples at 1:1000 (fig 2). Infect Immun (2016) ncbi
mouse monoclonal (Z014)
  • immunohistochemistry - frozen section; mouse; loading ...
  • flow cytometry; mouse; 1:50; loading ...; fig 6a
In order to identify annexin A2 as a binding partner of factor H, Invitrogen annexin II antibody (Invitrogen, Z014) was used in immunohistochemistry - frozen section on mouse samples and in flow cytometry on mouse samples at 1:50 (fig 6a). J Immunol (2016) ncbi
mouse monoclonal (Z014)
  • blocking or activating experiments; human; 25 ug/ml
In order to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells, Invitrogen annexin II antibody (Zymed Laboratories, 034400) was used in blocking or activating experiments on human samples at 25 ug/ml. J Biol Chem (2008) ncbi
mouse monoclonal (Z014)
  • blocking or activating experiments; human; fig 1
In order to show that the alpha(M)beta(2) regulates neutrophil-dependent fibrinolysis, Invitrogen annexin II antibody (Zymed, Z014) was used in blocking or activating experiments on human samples (fig 1). J Biol Chem (2004) ncbi
mouse monoclonal (Z014)
  • western blot; human; 0.125 ug/ml
  • western blot; mouse; 0.125 ug/ml; fig 6
In order to assess mediation of plasminogen-dependent matrix invasion by human monocytes by annexin II as enhanced expression by macrophages, Invitrogen annexin II antibody (Zymed, noca) was used in western blot on human samples at 0.125 ug/ml and in western blot on mouse samples at 0.125 ug/ml (fig 6). Blood (2004) ncbi
mouse monoclonal (Z014)
  • western blot; cow
In order to examine the effect of nitric oxide donors on the activities of annexin II tetramer, Invitrogen annexin II antibody (Zymed, 034400) was used in western blot on cow samples . Eur J Biochem (2002) ncbi
mouse monoclonal (Z014)
  • western blot; human; fig 1
In order to test if annexin II is released from syncytiotrophoblast microvillous plasma membranes and if it has any immunosuppressive effect, Invitrogen annexin II antibody (Zymed Laboratories, 03-4400) was used in western blot on human samples (fig 1). Am J Reprod Immunol (1997) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 2b
In order to find autoantigens in the skin of the affected limb using a mouse model of complex regional pain syndrome, Abcam annexin II antibody (Abcam, ab41803) was used in western blot on mouse samples at 1:1000 (fig 2b). Exp Neurol (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:100; loading ...; fig 1c
In order to use data-independent acquisition-mass spectrometry to study two mouse models of chronic pain, Abcam annexin II antibody (Abcam, ab41803) was used in western blot on mouse samples at 1:100 (fig 1c). Mol Cell Proteomics (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:250; fig 1
In order to study how Tyr23 phosphorylation regulates AnxA2 function, Abcam annexin II antibody (Abcam, ab41803) was used in immunocytochemistry on rat samples at 1:250 (fig 1). J Cell Sci (2016) ncbi
rabbit monoclonal (EPR13052(B))
  • western blot; human; fig 4
Abcam annexin II antibody (Abcam, ab178677) was used in western blot on human samples (fig 4). Arthritis Rheumatol (2015) ncbi
rabbit polyclonal
  • western blot; African green monkey
In order to perform a proteomic analysis of host proteins in Rift Valley fever virus virions and to study the particular significance of Hsp90, Abcam annexin II antibody (Abcam, ab41803) was used in western blot on African green monkey samples . PLoS ONE (2014) ncbi
Proteintech Group
mouse monoclonal (3H1B11)
  • western blot; mouse; 1:1000; fig 2
In order to determine inhibition of NLRC4-mediated inflammasome activation and binding to annexin A2 by the tick protein sialostatin L2, Proteintech Group annexin II antibody (Proteintech, 60051-1-1g) was used in western blot on mouse samples at 1:1000 (fig 2). Infect Immun (2016) ncbi
GeneTex
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, GeneTex annexin II antibody (GeneTex, GTX100046) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D11G2)
  • immunoprecipitation; mouse; loading ...; fig s5a
  • western blot; mouse; loading ...; fig s5a
Cell Signaling Technology annexin II antibody (Cell Signaling, 8235) was used in immunoprecipitation on mouse samples (fig s5a) and in western blot on mouse samples (fig s5a). Sci Rep (2018) ncbi
rabbit monoclonal (D11G2)
  • immunocytochemistry; human
In order to study retinal degeneration caused by disruption of the microtubule-binding and membrane-binding domains of CEP290, Cell Signaling Technology annexin II antibody (Cell Signaling, 8235) was used in immunocytochemistry on human samples . J Clin Invest (2013) ncbi
BD Biosciences
mouse monoclonal (5/Annexin II)
  • western blot; mouse; fig 4a
In order to describe extracellular vesicle subpopulations secreted by fat cells, BD Biosciences annexin II antibody (BD Biosciences, 610068) was used in western blot on mouse samples (fig 4a). J Extracell Vesicles (2017) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; rat; 1:1000; loading ...; fig 4b
In order to investigate the role of Ser25 phosphorylation in subcellular localization of Annexin A2 and its interaction with mRNP complexes, BD Biosciences annexin II antibody (BD Biosciences, 610069) was used in western blot on rat samples at 1:1000 (fig 4b). FEBS Open Bio (2017) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; human; loading ...; fig 1a
BD Biosciences annexin II antibody (BD Transduction Laboratories, 610068) was used in western blot on human samples (fig 1a). PLoS ONE (2016) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; human; loading ...; fig 4
BD Biosciences annexin II antibody (BD Transduction lab, 610069) was used in western blot on human samples (fig 4). Oncotarget (2016) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; rat; 1:1000; fig 6
In order to study how Tyr23 phosphorylation regulates AnxA2 function, BD Biosciences annexin II antibody (BD Biosciences, 610069) was used in western blot on rat samples at 1:1000 (fig 6). J Cell Sci (2016) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry; human; fig 3
  • immunohistochemistry; human; fig 5
  • western blot; human; fig 4
In order to demonstrate the transcriptional repression of ANXA2 by ERG in prostate epithelial cells and discuss how this interaction relates to prostate cancer, BD Biosciences annexin II antibody (BD Biosciences, 610068) was used in immunocytochemistry on human samples (fig 3), in immunohistochemistry on human samples (fig 5) and in western blot on human samples (fig 4). Mol Cancer Res (2015) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry knockout validation; mouse; fig 1
  • immunohistochemistry knockout validation; mouse; fig 1
BD Biosciences annexin II antibody (BD Pharmingen), 610069) was used in immunocytochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 1). Mol Cancer Res (2015) ncbi
mouse monoclonal (5/Annexin II)
  • immunohistochemistry - paraffin section; human; 1:1,000
  • western blot; human; 1:5,000
BD Biosciences annexin II antibody (BD Biosciences, 610069) was used in immunohistochemistry - paraffin section on human samples at 1:1,000 and in western blot on human samples at 1:5,000. Biomed Res (2014) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry; human
In order to demonstrate that Listeria monocytogenes uses efferocytosis to promote cell-to-cell spread during infection, BD Biosciences annexin II antibody (BD Biosciences, 610068) was used in immunocytochemistry on human samples . Nature (2014) ncbi
mouse monoclonal (6/p36/MAT1)
  • western blot; human; 1:4000
BD Biosciences annexin II antibody (BD Biosciences, 610532) was used in western blot on human samples at 1:4000. Clin Exp Immunol (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; 1:3000; loading ...; fig 2a
In order to assess if Annexin A2 is a key regulatory factor in epidermal growth factor-induced epithelial-mesenchymal transition in CaSki cervical cancer cells, Sigma-Aldrich annexin II antibody (Sigma-Aldrich, A2485) was used in western blot on human samples at 1:3000 (fig 2a). Oncol Lett (2017) ncbi
Articles Reviewed
  1. Hakuno D, Kimura M, Ito S, Satoh J, Nakashima Y, Horie T, et al. Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction. Sci Rep. 2018;8:16749 pubmed publisher
  2. Wan Y, Wang L, Zhu C, Zheng Q, Wang G, Tong J, et al. Aptamer-Conjugated Extracellular Nanovesicles for Targeted Drug Delivery. Cancer Res. 2018;78:798-808 pubmed publisher
  3. Durcin M, Fleury A, Taillebois E, Hilairet G, Krupova Z, Henry C, et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles. 2017;6:1305677 pubmed publisher
  4. Aukrust I, Rosenberg L, Ankerud M, Bertelsen V, Hollås H, Saraste J, et al. Post-translational modifications of Annexin A2 are linked to its association with perinuclear nonpolysomal mRNP complexes. FEBS Open Bio. 2017;7:160-173 pubmed publisher
  5. Cui L, Song J, Wu L, Cheng L, Chen A, Wang Y, et al. Role of Annexin A2 in the EGF-induced epithelial-mesenchymal transition in human CaSki cells. Oncol Lett. 2017;13:377-383 pubmed publisher
  6. Dismuke W, Klingeborn M, Stamer W. Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone. PLoS ONE. 2016;11:e0165326 pubmed publisher
  7. Tajerian M, Hung V, Khan H, Lahey L, Sun Y, Birklein F, et al. Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome. Exp Neurol. 2017;287:14-20 pubmed publisher
  8. Madureira P, Bharadwaj A, Bydoun M, Garant K, O Connell P, Lee P, et al. Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10. Oncotarget. 2016;7:47720-47737 pubmed publisher
  9. Rouwette T, Sondermann J, Avenali L, Gomez Varela D, Schmidt M. Standardized Profiling of The Membrane-Enriched Proteome of Mouse Dorsal Root Ganglia (DRG) Provides Novel Insights Into Chronic Pain. Mol Cell Proteomics. 2016;15:2152-68 pubmed publisher
  10. Wang X, Shaw D, Sakhon O, Snyder G, Sundberg E, Santambrogio L, et al. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Infect Immun. 2016;84:1796-1805 pubmed publisher
  11. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  12. Renner B, Tong H, Laskowski J, Jonscher K, Goetz L, Woolaver R, et al. Annexin A2 Enhances Complement Activation by Inhibiting Factor H. J Immunol. 2016;196:1355-65 pubmed publisher
  13. Cui H, Wang S, Miao J, Fu Z, Feng F, Wu J, et al. CD147 regulates cancer migration via direct interaction with Annexin A2 and DOCK3-β-catenin-WAVE2 signaling. Oncotarget. 2016;7:5613-29 pubmed publisher
  14. Grindheim A, Hollås H, Raddum A, Saraste J, Vedeler A. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2. J Cell Sci. 2016;129:314-28 pubmed publisher
  15. Lee K, Westin L, Kim J, Chang J, Oh Y, Amreen B, et al. Alteration by p11 of mGluR5 localization regulates depression-like behaviors. Mol Psychiatry. 2015;20:1546-56 pubmed publisher
  16. Moreau K, Ghislat G, Hochfeld W, Renna M, Zavodszky E, Runwal G, et al. Transcriptional regulation of Annexin A2 promotes starvation-induced autophagy. Nat Commun. 2015;6:8045 pubmed publisher
  17. Yalavarthi S, Gould T, Rao A, Mazza L, Morris A, Nuñez Alvarez C, et al. Release of neutrophil extracellular traps by neutrophils stimulated with antiphospholipid antibodies: a newly identified mechanism of thrombosis in the antiphospholipid syndrome. Arthritis Rheumatol. 2015;67:2990-3003 pubmed publisher
  18. Tristante E, Martínez C, Jiménez S, Mora L, Carballo F, Martínez Lacaci I, et al. Association of a characteristic membrane pattern of annexin A2 with high invasiveness and nodal status in colon adenocarcinoma. Transl Res. 2015;166:196-206 pubmed publisher
  19. Griner N, Young D, Chaudhary P, Mohamed A, Huang W, Chen Y, et al. ERG oncoprotein inhibits ANXA2 expression and function in prostate cancer. Mol Cancer Res. 2015;13:368-79 pubmed publisher
  20. Jung Y, Wang J, Lee E, McGee S, Berry J, Yumoto K, et al. Annexin 2-CXCL12 interactions regulate metastatic cell targeting and growth in the bone marrow. Mol Cancer Res. 2015;13:197-207 pubmed publisher
  21. Nuss J, Kehn Hall K, Benedict A, Costantino J, Ward M, Peyser B, et al. Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors. PLoS ONE. 2014;9:e93483 pubmed publisher
  22. Feng H, Li X, Chan V, Chen W. Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells. PLoS ONE. 2014;9:e95621 pubmed publisher
  23. Kobayashi M, Nagashio R, Ryuge S, Murakami Y, Yanagita K, Nakashima H, et al. Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues. Biomed Res. 2014;35:133-43 pubmed
  24. Czuczman M, Fattouh R, van Rijn J, Canadien V, Osborne S, Muise A, et al. Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature. 2014;509:230-4 pubmed publisher
  25. Zouk H, d Hennezel E, Du X, Ounissi Benkalha H, Piccirillo C, Polychronakos C. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus. Clin Exp Immunol. 2014;175:485-97 pubmed publisher
  26. Drivas T, Holzbaur E, Bennett J. Disruption of CEP290 microtubule/membrane-binding domains causes retinal degeneration. J Clin Invest. 2013;123:4525-39 pubmed publisher
  27. Bouwman F, Wang P, van Baak M, Saris W, Mariman E. Increased ?-oxidation with improved glucose uptake capacity in adipose tissue from obese after weight loss and maintenance. Obesity (Silver Spring). 2014;22:819-27 pubmed publisher
  28. Hastie C, Masters J, Moss S, Naaby Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem. 2008;283:12595-603 pubmed publisher
  29. Pluskota E, Soloviev D, Bdeir K, Cines D, Plow E. Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils. J Biol Chem. 2004;279:18063-72 pubmed
  30. Brownstein C, Deora A, Jacovina A, Weintraub R, Gertler M, Khan K, et al. Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages. Blood. 2004;103:317-24 pubmed
  31. Liu L, Enright E, Sun P, Tsai S, Mehta P, Beckman D, et al. Inactivation of annexin II tetramer by S-nitrosoglutathione. Eur J Biochem. 2002;269:4277-86 pubmed
  32. Aarli A, Kristoffersen E, Jensen T, Ulvestad E, Matre R. Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes. Am J Reprod Immunol. 1997;38:313-9 pubmed