This is a Validated Antibody Database (VAD) review about human alpha-adaptin, based on 28 published articles (read how Labome selects the articles), using alpha-adaptin antibody in all methods. It is aimed to help Labome visitors find the most suited alpha-adaptin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
alpha-adaptin synonym: ADTAA; AP2-ALPHA; CLAPA1

Invitrogen
mouse monoclonal (AC1-M11)
  • western blot; mouse; 1:500; loading ...; fig 5
Invitrogen alpha-adaptin antibody (ThermoFisher, MA3-061) was used in western blot on mouse samples at 1:500 (fig 5). elife (2020) ncbi
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 3a
Invitrogen alpha-adaptin antibody (Dianova, MA3-061) was used in western blot on mouse samples (fig 3a). Neuroscience (2018) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; loading ...; fig 3d
In order to investigate the adhesion structures in 3-D cell migration, Invitrogen alpha-adaptin antibody (ThermoFisher, AP6) was used in immunocytochemistry on human samples (fig 3d). Science (2017) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig 2b
  • western blot; human; fig 1b
  • immunocytochemistry; pigs ; loading ...; fig 5d
  • western blot; pigs ; loading ...; fig 5a
In order to study amyloid precursor protein Tyr phosphorylation in mouse model of Alzheimer's disease, Invitrogen alpha-adaptin antibody (Thermo Fisher, MA1-064) was used in immunocytochemistry on human samples (fig 2b), in western blot on human samples (fig 1b), in immunocytochemistry on pigs samples (fig 5d) and in western blot on pigs samples (fig 5a). Front Mol Neurosci (2017) ncbi
mouse monoclonal (AC1-M11)
  • immunoprecipitation; human; fig s1a
  • western blot; human; fig s1a
In order to elucidate how adaptor protein 2 complexes regulate clathrin-coated pit assembly, Invitrogen alpha-adaptin antibody (Thermo Fisher, AC1-M11) was used in immunoprecipitation on human samples (fig s1a) and in western blot on human samples (fig s1a). J Cell Biol (2017) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; loading ...; fig 2d
In order to assess the effect of three alpha-tubulin acetyltransferase 1-specific shRNAs on proliferation and morphology in HeLa and A549 cell lines, Invitrogen alpha-adaptin antibody (Thermo Fisher, MA1-064) was used in immunocytochemistry on human samples (fig 2d). Cell Death Discov (2016) ncbi
mouse monoclonal (AC1-M11)
  • immunocytochemistry; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 4b
In order to propose that ikarugamycin is a useful reagent to study routes of endocytic trafficking, Invitrogen alpha-adaptin antibody (Thermo Scientific, MA3-061) was used in immunocytochemistry on human samples (fig 5a) and in western blot on human samples (fig 4b). Traffic (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig s5
In order to monitor the interaction beteween integrin alpha chain and AP2 and how selective integrin endocytosis drives the interaction, Invitrogen alpha-adaptin antibody (Pierce, MA1-064) was used in immunocytochemistry on human samples (fig s5). Nat Struct Mol Biol (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; rat; fig 4
In order to characterize inhibition of clathrin-mediated and bulk endocytosis by synaptotagmin-11, Invitrogen alpha-adaptin antibody (Thermo Scientific, MA1-064) was used in immunocytochemistry on rat samples (fig 4). EMBO Rep (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; African green monkey; fig s2d
  • western blot; human; fig s1f
In order to identify and characterize a new endocytic route that is called fast endophilin-mediated endocytosis, Invitrogen alpha-adaptin antibody (Thermo, MA1-064) was used in immunocytochemistry on African green monkey samples (fig s2d) and in western blot on human samples (fig s1f). Nature (2015) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; mouse; fig 2
In order to study the off-target activity of dynamin inhibitors using cells deleted for dynamins 1-3, Invitrogen alpha-adaptin antibody (Affinity Bioreagents, AP6) was used in immunocytochemistry on mouse samples (fig 2). J Cell Sci (2013) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig 3
In order to develop an array-based multiplexed analysis of signaling pathways, Invitrogen alpha-adaptin antibody (Affinity BioReagents, MA1-064) was used in immunocytochemistry on human samples (fig 3). Mol Cell Proteomics (2007) ncbi
mouse monoclonal (AC1-M11)
  • western blot; rat; loading ...; fig 3a
In order to find factors that interact with N-myristoylated Arf6, Invitrogen alpha-adaptin antibody (Affinity Bioreagents, AC1-M11) was used in western blot on rat samples (fig 3a). Methods Enzymol (2005) ncbi
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 2c
In order to explore the effects of tensile stress on osteoblast differentiation and osteogenesis, Invitrogen alpha-adaptin antibody (Affinity Bioreagents, AC1-M11) was used in western blot on mouse samples (fig 2c). J Cell Physiol (2003) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-8)
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3e
Santa Cruz Biotechnology alpha-adaptin antibody (Santa Cruz, SC-17771) was used in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3e). Dev Cell (2019) ncbi
mouse monoclonal (C-8)
  • western blot; human; loading ...; fig sf2
In order to research the mechanisms of mitochondrial division, Santa Cruz Biotechnology alpha-adaptin antibody (Santa Cruz Biotechnology, sc-17771) was used in western blot on human samples (fig sf2). Nature (2016) ncbi
mouse monoclonal (C-8)
  • western blot; mouse; loading ...; fig 1b
In order to show that syntaxin-17 is required for the delivery of stress-induced PINK1/parkin-dependent mitochondrial-derived vesicles to the late endosome/lysosome, Santa Cruz Biotechnology alpha-adaptin antibody (Santa Cruz, sc-17771) was used in western blot on mouse samples (fig 1b). J Cell Biol (2016) ncbi
mouse monoclonal (C-8)
  • western blot; mouse; 1:1000; loading ...; fig 1a
Santa Cruz Biotechnology alpha-adaptin antibody (Santa cruz, sc-17771) was used in western blot on mouse samples at 1:1000 (fig 1a). Eneuro (2016) ncbi
mouse monoclonal (C-8)
  • western blot; human; 1:500; loading ...; fig 9e
Santa Cruz Biotechnology alpha-adaptin antibody (Santa Cruz Biotechnology, sc-17771) was used in western blot on human samples at 1:500 (fig 9e). elife (2014) ncbi
Abcam
mouse monoclonal (AP6)
  • immunohistochemistry; mouse; 1:300; loading ...; fig ev4p
Abcam alpha-adaptin antibody (Abcam, Ab2730) was used in immunohistochemistry on mouse samples at 1:300 (fig ev4p). EMBO Rep (2020) ncbi
mouse monoclonal (AP6)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4c
Abcam alpha-adaptin antibody (Abcam, ab2730) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 4c). Cell Rep (2019) ncbi
domestic goat polyclonal
  • immunoprecipitation; mouse; 1:500; loading ...; fig 2d
  • immunoprecipitation; human; 1:500; loading ...; fig s3e
Abcam alpha-adaptin antibody (Abcam, ab189995) was used in immunoprecipitation on mouse samples at 1:500 (fig 2d) and in immunoprecipitation on human samples at 1:500 (fig s3e). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C83E10)
  • western blot; mouse; 1:1000; loading ...; fig 6g
Cell Signaling Technology alpha-adaptin antibody (CST, 3215) was used in western blot on mouse samples at 1:1000 (fig 6g). Nat Commun (2022) ncbi
domestic rabbit monoclonal (C83E10)
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse; 1:1000
In order to study the development of fatal cardiac hypertrophy and arrrhythmia in mice overexpressing miRNA-17-92 in heart and smooth muscle, Cell Signaling Technology alpha-adaptin antibody (Cell Signaling Technology, 3215) was used in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples at 1:1000. FASEB J (2013) ncbi
MilliporeSigma
mouse monoclonal (100/2)
  • western blot; mouse; loading ...; fig 3a
In order to present the role of cathepsin D and its newly identified transport receptor SEZ6L2 on neurite outgrowth, MilliporeSigma alpha-adaptin antibody (Sigma, 100/2) was used in western blot on mouse samples (fig 3a). J Cell Sci (2016) ncbi
mouse monoclonal (100/2)
  • western blot; rat
MilliporeSigma alpha-adaptin antibody (Sigma, 100/2) was used in western blot on rat samples . J Comp Neurol (2007) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E4)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5i
In order to discuss the role of sonic hedgehog signaling and alcohol exposure to the development of septo-optic dysplasia, Developmental Studies Hybridoma Bank alpha-adaptin antibody (DSHB, 5E4) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5i). Dis Model Mech (2017) ncbi
mouse monoclonal (5E4)
  • immunohistochemistry; mouse; 1:200
Developmental Studies Hybridoma Bank alpha-adaptin antibody (DSHB, 5E4) was used in immunohistochemistry on mouse samples at 1:200. Mol Brain (2015) ncbi
Articles Reviewed
  1. Liu H, He J, Bagheri Yarmand R, Li Z, Liu R, Wang Z, et al. Osteocyte CIITA aggravates osteolytic bone lesions in myeloma. Nat Commun. 2022;13:3684 pubmed publisher
  2. Bera S, Camblor Perujo S, Calleja Barca E, Negrete Hurtado A, Racho J, de Bruyckere E, et al. AP-2 reduces amyloidogenesis by promoting BACE1 trafficking and degradation in neurons. EMBO Rep. 2020;21:e47954 pubmed publisher
  3. Evans H, Bodea L, Götz J. Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation. elife. 2020;9: pubmed publisher
  4. Gulbranson D, Crisman L, Lee M, Ouyang Y, Menasche B, Demmitt B, et al. AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis. Dev Cell. 2019;50:436-446.e5 pubmed publisher
  5. Wagner W, Lippmann K, Heisler F, Gromova K, Lombino F, Roesler M, et al. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression. Cell Rep. 2019;28:11-20.e9 pubmed publisher
  6. Richter K, Schmutz I, Darna M, Zander J, Chavan R, Albrecht U, et al. VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context. Neuroscience. 2018;371:29-37 pubmed publisher
  7. Elkhatib N, Bresteau E, Baschieri F, Rioja A, van Niel G, Vassilopoulos S, et al. Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science. 2017;356: pubmed publisher
  8. Poulsen E, Iannuzzi F, Rasmussen H, Maier T, Enghild J, Jørgensen A, et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci. 2017;10:59 pubmed publisher
  9. Bagh M, Peng S, Chandra G, Zhang Z, Singh S, Pattabiraman N, et al. Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model. Nat Commun. 2017;8:14612 pubmed publisher
  10. Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed publisher
  11. Kahn B, Corman T, Lovelace K, Hong M, Krauss R, Epstein D. Prenatal ethanol exposure in mice phenocopies Cdon mutation by impeding Shh function in the etiology of optic nerve hypoplasia. Dis Model Mech. 2017;10:29-37 pubmed publisher
  12. Lee J, Westrate L, Wu H, Page C, Voeltz G. Multiple dynamin family members collaborate to drive mitochondrial division. Nature. 2016;540:139-143 pubmed publisher
  13. Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
  14. McLelland G, Lee S, McBride H, Fon E. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol. 2016;214:275-91 pubmed publisher
  15. Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed publisher
  16. daSilva L, Wall M, P de Almeida L, Wauters S, Januário Y, Muller J, et al. Activity-Regulated Cytoskeleton-Associated Protein Controls AMPAR Endocytosis through a Direct Interaction with Clathrin-Adaptor Protein 2. Eneuro. 2016;3: pubmed publisher
  17. De Franceschi N, Arjonen A, Elkhatib N, Denessiouk K, Wrobel A, Wilson T, et al. Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2. Nat Struct Mol Biol. 2016;23:172-9 pubmed publisher
  18. Boonen M, Staudt C, Gilis F, Oorschot V, Klumperman J, Jadot M. Cathepsin D and its newly identified transport receptor SEZ6L2 can modulate neurite outgrowth. J Cell Sci. 2016;129:557-68 pubmed publisher
  19. Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
  20. Jin K, Jiang H, Xiao D, Zou M, Zhu J, Xiang M. Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis. Mol Brain. 2015;8:28 pubmed publisher
  21. Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
  22. Umasankar P, Ma L, Thieman J, Jha A, Doray B, Watkins S, et al. A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing. elife. 2014;3: pubmed publisher
  23. Park R, Shen H, Liu L, Liu X, Ferguson S, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. J Cell Sci. 2013;126:5305-12 pubmed publisher
  24. Danielson L, Park D, Rotllan N, Chamorro Jorganes A, Guijarro M, Fernandez Hernando C, et al. Cardiovascular dysregulation of miR-17-92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. FASEB J. 2013;27:1460-7 pubmed publisher
  25. Petralia R, Yao P. AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles. J Comp Neurol. 2007;504:314-27 pubmed
  26. Zanardi A, Giorgetti L, Botrugno O, Minucci S, Milani P, Pelicci P, et al. Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells. Mol Cell Proteomics. 2007;6:939-47 pubmed
  27. Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
  28. Shimomura J, Ishibashi O, Ikegame M, Yoshizawa T, Ejiri S, Noda T, et al. Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. J Cell Physiol. 2003;195:488-96 pubmed