This is a Validated Antibody Database (VAD) review about human alpha B-crystallin, based on 21 published articles (read how Labome selects the articles), using alpha B-crystallin antibody in all methods. It is aimed to help Labome visitors find the most suited alpha B-crystallin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
alpha B-crystallin synonym: CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; alpha-crystallin B chain; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; renal carcinoma antigen NY-REN-27; rosenthal fiber component

Abcam
mouse monoclonal (1B6.1-3G4)
  • immunocytochemistry; mouse; loading ...; fig 6c
In order to determine the role of CRYAB in primary demyelinating events, Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in immunocytochemistry on mouse samples (fig 6c). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 5b
In order to determine the role of CRYAB in primary demyelinating events, Abcam alpha B-crystallin antibody (Abcam, ab5598) was used in immunohistochemistry - free floating section on mouse samples at 1:500 (fig 5b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 5a
In order to determine the role of CRYAB in primary demyelinating events, Abcam alpha B-crystallin antibody (Abcam, ab5577) was used in immunohistochemistry - free floating section on mouse samples at 1:500 (fig 5a). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (1B6.1-3G4)
  • immunocytochemistry; human; fig 4
Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in immunocytochemistry on human samples (fig 4). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4
In order to investigate the modulator of skeletal muscle sarcomeric morphometry associated to modulation of protein-protein interactions by O-GlcNAcylation, Abcam alpha B-crystallin antibody (Abcam, ab13497) was used in western blot on mouse samples (fig 4). Biochim Biophys Acta (2016) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; rat; 1:1000; fig 1
Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in western blot on rat samples at 1:1000 (fig 1). Int J Mol Med (2016) ncbi
mouse monoclonal (1B6.1-3G4)
  • immunohistochemistry - frozen section; human; loading ...; fig 6a
In order to design Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells, Abcam alpha B-crystallin antibody (Abcam, 1B6.1-3G4) was used in immunohistochemistry - frozen section on human samples (fig 6a). Nat Methods (2016) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; rat; 1:1000; loading ...; tbl 1
Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in western blot on rat samples at 1:1000 (tbl 1). PLoS ONE (2015) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; rat; 1:5000; loading ...; fig 4a
In order to study the adaptive response of the retina to short-term high fructose treatment, Abcam alpha B-crystallin antibody (Abcam, 1B6.1-3G4) was used in western blot on rat samples at 1:5000 (fig 4a). Exp Eye Res (2015) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; human; fig 1
Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in western blot on human samples (fig 1). Mol Cell Proteomics (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Abcam alpha B-crystallin antibody (Santa Cruz, ab13497) was used in immunocytochemistry on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; cow
In order to study the role of small heat shock proteins in protecting bovine skeletal myofibrils from post-mortem proteolytic degradation, Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in western blot on cow samples . Meat Sci (2014) ncbi
mouse monoclonal (1B6.1-3G4)
  • western blot; mouse
Abcam alpha B-crystallin antibody (Abcam, ab13496) was used in western blot on mouse samples . Int J Mol Med (2013) ncbi
Invitrogen
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...
In order to determine the role of CRYAB in primary demyelinating events, Invitrogen alpha B-crystallin antibody (Thermo Fisher, PA1-011) was used in immunoprecipitation on mouse samples . Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...
In order to determine the role of CRYAB in primary demyelinating events, Invitrogen alpha B-crystallin antibody (Thermo Fisher, PA1-012) was used in immunoprecipitation on mouse samples . Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...
In order to determine the role of CRYAB in primary demyelinating events, Invitrogen alpha B-crystallin antibody (Thermo Fisher, PA1-010) was used in immunoprecipitation on mouse samples . Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; loading ...
In order to determine the role of CRYAB in primary demyelinating events, Invitrogen alpha B-crystallin antibody (Thermo Fisher, PA1-20795) was used in immunoprecipitation on mouse samples . Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (1D11C6E6)
  • immunocytochemistry; human; 1:200; fig 1a
In order to examine the involvement of melanopsin in melatonin regulation, Invitrogen alpha B-crystallin antibody (Thermo Fisher, 1D11C6E6) was used in immunocytochemistry on human samples at 1:200 (fig 1a). Exp Eye Res (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; fig 1
In order to study the composition of the protein aggregate hallmark of Alexander disease called rosenthal fibers, Invitrogen alpha B-crystallin antibody (Pierce, PA1-16950) was used in immunohistochemistry on mouse samples at 1:1000 (fig 1). J Proteome Res (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100
  • western blot; rat; 1:2000
In order to study the role of alphaBC under chronic hyperglycemia in rat skeletal muscle, Invitrogen alpha B-crystallin antibody (Thermo, PA1-010) was used in immunohistochemistry - paraffin section on rat samples at 1:100 and in western blot on rat samples at 1:2000. IUBMB Life (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100
  • western blot; rat; 1:2000
In order to study the role of alphaBC under chronic hyperglycemia in rat skeletal muscle, Invitrogen alpha B-crystallin antibody (Thermo, PA1-012) was used in immunohistochemistry - paraffin section on rat samples at 1:100 and in western blot on rat samples at 1:2000. IUBMB Life (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100
  • western blot; rat; 1:2000
In order to study the role of alphaBC under chronic hyperglycemia in rat skeletal muscle, Invitrogen alpha B-crystallin antibody (Thermo, PA1-011) was used in immunohistochemistry - paraffin section on rat samples at 1:100 and in western blot on rat samples at 1:2000. IUBMB Life (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
In order to examine the expression of sHsp under chronic hyperglycemic conditions in rat heart, Invitrogen alpha B-crystallin antibody (Thermo Scientific-Pierce, PA1-011) was used in western blot on rat samples at 1:2000. Arch Biochem Biophys (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
In order to examine the expression of sHsp under chronic hyperglycemic conditions in rat heart, Invitrogen alpha B-crystallin antibody (Thermo Scientific-Pierce, PA1-010) was used in western blot on rat samples at 1:2000. Arch Biochem Biophys (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:100
  • immunoprecipitation; rat; 20 ug
  • western blot; rat; 1:2000
In order to examine the expression of sHsp under chronic hyperglycemic conditions in rat heart, Invitrogen alpha B-crystallin antibody (Thermo Scientific-Pierce, PA1-012) was used in immunohistochemistry - paraffin section on rat samples at 1:100, in immunoprecipitation on rat samples at 20 ug and in western blot on rat samples at 1:2000. Arch Biochem Biophys (2014) ncbi
OriGene
mouse monoclonal (OTI5D8)
  • immunocytochemistry; human; loading ...; fig 2d
In order to research a paradigm for generation of all major ectodermal lineages from human pluripotent stem cells, OriGene alpha B-crystallin antibody (OriGene, CF500680) was used in immunocytochemistry on human samples (fig 2d). Cell Stem Cell (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-7)
  • western blot; mouse; 1:2000
Santa Cruz Biotechnology alpha B-crystallin antibody (Santa Cruz Biotechnology, sc-137143) was used in western blot on mouse samples at 1:2000. Muscle Nerve (2015) ncbi
EMD Millipore
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
  • western blot; mouse; 1:1000; loading ...; fig 1a
EMD Millipore alpha B-crystallin antibody (Millipore, ABN185) was used in immunohistochemistry on mouse samples at 1:200 (fig 1b) and in western blot on mouse samples at 1:1000 (fig 1a). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:2000; loading ...; fig 6
In order to evaluate chronic traumatic encephalopathy-like changes during routine neuropathology services, EMD Millipore alpha B-crystallin antibody (Millipore, ABN185) was used in immunohistochemistry - paraffin section on human samples at 1:2000 (fig 6). J Neuropathol Exp Neurol (2016) ncbi
Leica Biosystems
mouse monoclonal
  • immunohistochemistry - frozen section; mouse
Leica Biosystems alpha B-crystallin antibody (Novocastra, NCL-abCRYS-512) was used in immunohistochemistry - frozen section on mouse samples . Am J Pathol (2013) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (CPTC-CRYAB-3)
  • immunohistochemistry - frozen section; mouse
Developmental Studies Hybridoma Bank alpha B-crystallin antibody (DSHB, CPTC-CRYAB-3) was used in immunohistochemistry - frozen section on mouse samples . Proteome Sci (2014) ncbi
Articles Reviewed
  1. Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, et al. A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages. Cell Stem Cell. 2017;21:399-410.e7 pubmed publisher
  2. Kuipers H, Yoon J, van Horssen J, Han M, Bollyky P, Palmer T, et al. Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A. 2017;114:E1745-E1754 pubmed publisher
  3. Lim E, Nakanishi S, Hoghooghi V, Eaton S, Palmer A, Frederick A, et al. AlphaB-crystallin regulates remyelination after peripheral nerve injury. Proc Natl Acad Sci U S A. 2017;114:E1707-E1716 pubmed publisher
  4. Alkozi H, Wang X, Pérez de Lara M, Pintor J. Presence of melanopsin in human crystalline lens epithelial cells and its role in melatonin synthesis. Exp Eye Res. 2017;154:168-176 pubmed publisher
  5. Noy S, Krawitz S, Del Bigio M. Chronic Traumatic Encephalopathy-Like Abnormalities in a Routine Neuropathology Service. J Neuropathol Exp Neurol. 2016;75:1145-1154 pubmed publisher
  6. Zhang K, Zhu X, Lu Y. Effect of Mild Heating on Human Lens Epithelial Cells: A Possible Model of Lens Aging. Sci Rep. 2016;6:33917 pubmed publisher
  7. Lambert M, Richard E, Duban Deweer S, Krzewinski F, Deracinois B, Dupont E, et al. O-GlcNAcylation is a key modulator of skeletal muscle sarcomeric morphometry associated to modulation of protein-protein interactions. Biochim Biophys Acta. 2016;1860:2017-30 pubmed publisher
  8. Heaven M, Flint D, Randall S, Sosunov A, Wilson L, Barnes S, et al. Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease. J Proteome Res. 2016;15:2265-82 pubmed publisher
  9. Tang S, Chen H, Cheng Y, Nasir M, Kemper N, Bao E. The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress. Int J Mol Med. 2016;37:56-62 pubmed publisher
  10. Thomsen E, Mich J, Yao Z, Hodge R, Doyle A, Jang S, et al. Fixed single-cell transcriptomic characterization of human radial glial diversity. Nat Methods. 2016;13:87-93 pubmed publisher
  11. Herold S, Kumar P, Wichert S, Kretzschmar B, Bähr M, Rossner M, et al. Neurodegeneration in Autoimmune Optic Neuritis Is Associated with Altered APP Cleavage in Neurons and Up-Regulation of p53. PLoS ONE. 2015;10:e0138852 pubmed publisher
  12. Thierry M, Pasquis B, Buteau B, Fourgeux C, Dembele D, Leclère L, et al. Early adaptive response of the retina to a pro-diabetogenic diet: Impairment of cone response and gene expression changes in high-fructose fed rats. Exp Eye Res. 2015;135:37-46 pubmed publisher
  13. Gago Fuentes R, Fernández Puente P, Megias D, Carpintero Fernández P, Mateos J, Acea B, et al. Proteomic Analysis of Connexin 43 Reveals Novel Interactors Related to Osteoarthritis. Mol Cell Proteomics. 2015;14:1831-45 pubmed publisher
  14. Reddy V, Jakhotia S, Reddy P, Reddy G. Hyperglycemia induced expression, phosphorylation, and translocation of αB-crystallin in rat skeletal muscle. IUBMB Life. 2015;67:291-9 pubmed publisher
  15. Wang F, Zhang P, Liu H, Fan M, Chen X. Proteomic analysis of mouse soleus muscles affected by hindlimb unloading and reloading. Muscle Nerve. 2015;52:803-11 pubmed publisher
  16. Anchan R, Lachke S, Gerami Naini B, Lindsey J, Ng N, Naber C, et al. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS ONE. 2014;9:e115106 pubmed publisher
  17. Can T, Faas L, Ashford D, Dowle A, Thomas J, O Toole P, et al. Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections. Proteome Sci. 2014;12:25 pubmed publisher
  18. Reddy V, Kumar C, Raghu G, Reddy G. Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions. Arch Biochem Biophys. 2014;558:1-9 pubmed publisher
  19. Lomiwes D, Hurst S, Dobbie P, Frost D, Hurst R, Young O, et al. The protection of bovine skeletal myofibrils from proteolytic damage post mortem by small heat shock proteins. Meat Sci. 2014;97:548-57 pubmed publisher
  20. Freret M, Drouot L, Obry A, Ahmed Lacheheb S, Dauly C, Adriouch S, et al. Overexpression of MHC class I in muscle of lymphocyte-deficient mice causes a severe myopathy with induction of the unfolded protein response. Am J Pathol. 2013;183:893-904 pubmed publisher
  21. Carberry S, Brinkmeier H, Zhang Y, Winkler C, Ohlendieck K. Comparative proteomic profiling of soleus, extensor digitorum longus, flexor digitorum brevis and interosseus muscles from the mdx mouse model of Duchenne muscular dystrophy. Int J Mol Med. 2013;32:544-56 pubmed publisher