This is a Validated Antibody Database (VAD) review about human ZO 3, based on 10 published articles (read how Labome selects the articles), using ZO 3 antibody in all methods. It is aimed to help Labome visitors find the most suited ZO 3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ZO 3 synonym: ZO-3; ZO3; tight junction protein ZO-3; tight junction protein 3 (zona occludens 3); zona occludens protein 3; zonula occludens protein 3

Invitrogen
rabbit polyclonal
  • immunocytochemistry; dog
In order to characterize TALEN-mediated claudin-2 knockout in MDCK cells, Invitrogen ZO 3 antibody (Invitrogen, 36-4100) was used in immunocytochemistry on dog samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:500; fig 3
  • western blot; mouse; 1:500; fig 6
  • immunohistochemistry - paraffin section; rat; 1:500; fig 7
In order to study the contribution of ZO-1, -2, and -3 on collecting duct cell proliferation, Invitrogen ZO 3 antibody (Invitrogen, 36-4100) was used in immunocytochemistry on mouse samples at 1:500 (fig 3), in western blot on mouse samples at 1:500 (fig 6) and in immunohistochemistry - paraffin section on rat samples at 1:500 (fig 7). Cell Cycle (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; dog
  • western blot; dog
In order to characterize the TALEN-mediated ZO-1 knockout in MDCK cells, Invitrogen ZO 3 antibody (Invitrogen, 36-4100) was used in immunocytochemistry on dog samples and in western blot on dog samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; dog
In order to study the role of ZO proteins in the regulation of transcription factor DbpA/ZONAB, Invitrogen ZO 3 antibody (Zymed, 36-4100) was used in western blot on dog samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1.25 ug/ml; fig 3
  • western blot; human; 2.5 ug/ml; fig 3
In order to study the molecular processes involved in renal epithelial proximal-tubule monolayer maturation, Invitrogen ZO 3 antibody (Zymed, 36-4000) was used in immunocytochemistry on human samples at 1.25 ug/ml (fig 3) and in western blot on human samples at 2.5 ug/ml (fig 3). Mol Cell Biol (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:50; fig 7
In order to study barrier integrity and tight junction protein expression using in vitro lung epithelial barrier model, Invitrogen ZO 3 antibody (Invitrogen, ZMD.260) was used in immunocytochemistry on human samples at 1:50 (fig 7). Immunobiology (2011) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 4F
In order to develop an in vitro model of the respiratory tract wall, Invitrogen ZO 3 antibody (Zymed, 36-4100) was used in immunocytochemistry on human samples at 1:100 (fig 4F). J Aerosol Med (2006) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 3
In order to develop and characterize a triple co-culture model of the human airway barrier, Invitrogen ZO 3 antibody (Zymed, 36-4,100) was used in immunocytochemistry on human samples at 1:100 (fig 3). Am J Respir Cell Mol Biol (2005) ncbi
Cell Signaling Technology
rabbit monoclonal (D57G7)
  • western blot; pig; 1:2000; fig 6A
In order to assess the effects of deoxynivalenol and its derivatives on intestinal tight junction proteins, Cell Signaling Technology ZO 3 antibody (Cell Signaling, D57G7) was used in western blot on pig samples at 1:2000 (fig 6A). Toxins (Basel) (2016) ncbi
rabbit monoclonal (D57G7)
  • immunohistochemistry; human; fig 6
In order to elucidate the contributions of cell-cell adhesion proteins to intercellular force and epithelial tissue dynamics, Cell Signaling Technology ZO 3 antibody (Cell Signaling, 3704) was used in immunohistochemistry on human samples (fig 6). Nat Cell Biol (2015) ncbi
Articles Reviewed
  1. Springler A, Hessenberger S, Schatzmayr G, Mayer E. Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network. Toxins (Basel). 2016;8: pubmed publisher
  2. Bazellières E, Conte V, Elosegui Artola A, Serra Picamal X, Bintanel Morcillo M, Roca Cusachs P, et al. Control of cell-cell forces and collective cell dynamics by the intercellular adhesome. Nat Cell Biol. 2015;17:409-20 pubmed publisher
  3. Tokuda S, Furuse M. Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells. PLoS ONE. 2015;10:e0119869 pubmed publisher
  4. Qiao X, Roth I, Féraille E, Hasler U. Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion. Cell Cycle. 2014;13:3059-75 pubmed publisher
  5. Tokuda S, Higashi T, Furuse M. ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape. PLoS ONE. 2014;9:e104994 pubmed publisher
  6. Spadaro D, Tapia R, Jond L, Sudol M, Fanning A, Citi S. ZO proteins redundantly regulate the transcription factor DbpA/ZONAB. J Biol Chem. 2014;289:22500-11 pubmed publisher
  7. Aschauer L, Gruber L, Pfaller W, Limonciel A, Athersuch T, Cavill R, et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol. 2013;33:2535-50 pubmed publisher
  8. Blank F, Wehrli M, Lehmann A, Baum O, Gehr P, von Garnier C, et al. Macrophages and dendritic cells express tight junction proteins and exchange particles in an in vitro model of the human airway wall. Immunobiology. 2011;216:86-95 pubmed publisher
  9. Blank F, Rothen Rutishauser B, Schurch S, Gehr P. An optimized in vitro model of the respiratory tract wall to study particle cell interactions. J Aerosol Med. 2006;19:392-405 pubmed
  10. Rothen Rutishauser B, Kiama S, Gehr P. A three-dimensional cellular model of the human respiratory tract to study the interaction with particles. Am J Respir Cell Mol Biol. 2005;32:281-9 pubmed