This is a Validated Antibody Database (VAD) review about human XRN2, based on 8 published articles (read how Labome selects the articles), using XRN2 antibody in all methods. It is aimed to help Labome visitors find the most suited XRN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
XRN2 synonym: 5'-3' exoribonuclease 2; DHP protein; Dhm1-like protein

Bethyl
rabbit polyclonal
  • proximity ligation assay; human; 1:200; loading ...; fig 3c
  • immunocytochemistry; human; 1:200; loading ...; fig 3b
  • western blot; human; 1:5000; loading ...; fig s7b
Bethyl XRN2 antibody (Bethyl Laboratories, A301-103A) was used in proximity ligation assay on human samples at 1:200 (fig 3c), in immunocytochemistry on human samples at 1:200 (fig 3b) and in western blot on human samples at 1:5000 (fig s7b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:2000; fig s7
In order to study melanoma addiction to RNA SAMMSON long non-coding region, Bethyl XRN2 antibody (Bethyl Laboratories, A301-103A) was used in western blot on human samples at 1:2000 (fig s7). Nature (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl XRN2 antibody (Bethyl, A301-103A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2a
In order to identify residues of Y14 that are needed for binding to the mRNA cap, Bethyl XRN2 antibody (Bethyl Laboratories, A301-103A) was used in western blot on human samples (fig 2a). J Biol Chem (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
  • western blot; human; fig 2
Bethyl XRN2 antibody (Bethyl Laboratories, A301-103A) was used in chromatin immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 2). Genes Dev (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:5000; fig s6
Bethyl XRN2 antibody (Bethyl Laboratories, A301-102A) was used in western blot on human samples at 1:5000 (fig s6). Cell Rep (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5c
In order to study the regulation of premature cleavage and polyadenylation by TRAP150, Bethyl XRN2 antibody (Bethyl, A301-102A) was used in western blot on human samples (fig 5c). Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:10,000
Bethyl XRN2 antibody (Bethyl, A301-101A) was used in western blot on human samples at 1:10,000. Cell Host Microbe (2014) ncbi
Articles Reviewed
  1. Coccia M, Rossi A, Riccio A, Trotta E, Santoro M. Human NF-κB repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance. Proc Natl Acad Sci U S A. 2017;114:1045-1050 pubmed publisher
  2. Leucci E, Vendramin R, Spinazzi M, Laurette P, Fiers M, Wouters J, et al. Melanoma addiction to the long non-coding RNA SAMMSON. Nature. 2016;531:518-22 pubmed publisher
  3. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  4. Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
  5. Sansó M, Levin R, Lipp J, Wang V, Greifenberg A, Quezada E, et al. P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates. Genes Dev. 2016;30:117-31 pubmed publisher
  6. Lubas M, Andersen P, Schein A, Dziembowski A, Kudla G, Jensen T. The human nuclear exosome targeting complex is loaded onto newly synthesized RNA to direct early ribonucleolysis. Cell Rep. 2015;10:178-92 pubmed publisher
  7. Lee K, Tarn W. TRAP150 activates splicing in composite terminal exons. Nucleic Acids Res. 2014;42:12822-32 pubmed publisher
  8. Sedano C, Sarnow P. Hepatitis C virus subverts liver-specific miR-122 to protect the viral genome from exoribonuclease Xrn2. Cell Host Microbe. 2014;16:257-264 pubmed publisher