This is a Validated Antibody Database (VAD) review about human XPF, based on 9 published articles (read how Labome selects the articles), using XPF antibody in all methods. It is aimed to help Labome visitors find the most suited XPF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
XPF synonym: ERCC11; FANCQ; RAD1; XFEPS; XPF; DNA repair endonuclease XPF; DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; excision-repair, complementing defective, in Chinese hamster; xeroderma pigmentosum group F-complementing protein; xeroderma pigmentosum, complementation group F

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (3F2/3)
  • immunocytochemistry knockout validation; human; loading ...; fig 1b
  • western blot knockout validation; human; loading ...; fig 1a
Santa Cruz Biotechnology XPF antibody (Santa Cruz, sc-136153) was used in immunocytochemistry knockout validation on human samples (fig 1b) and in western blot knockout validation on human samples (fig 1a). Nucleic Acids Res (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3F2/3)
  • immunocytochemistry knockout validation; human; loading ...; fig 1b
  • western blot knockout validation; human; loading ...; fig 1a
Santa Cruz Biotechnology XPF antibody (Santa Cruz, sc-136153) was used in immunocytochemistry knockout validation on human samples (fig 1b) and in western blot knockout validation on human samples (fig 1a). Nucleic Acids Res (2018) ncbi
mouse monoclonal (3F2/3)
  • western blot; human; 1:500; loading ...; fig 2a
Santa Cruz Biotechnology XPF antibody (Santa Cruz Biotechnology, sc-136153) was used in western blot on human samples at 1:500 (fig 2a). Nucleic Acids Res (2018) ncbi
mouse monoclonal (3F2/3)
  • immunocytochemistry; mouse; 1:100
In order to elucidate ubiquitylation of XPC, Santa Cruz Biotechnology XPF antibody (Santa Cruz, 3F2) was used in immunocytochemistry on mouse samples at 1:100. Nat Commun (2015) ncbi
Invitrogen
mouse monoclonal (51)
  • western blot; human; 1:500; fig 3ai
Invitrogen XPF antibody (Thermo Fisher, MA5-12060) was used in western blot on human samples at 1:500 (fig 3ai). Genome Biol (2018) ncbi
mouse monoclonal (219)
  • western blot; human; 1:500; fig 8
In order to study the role of Mus81 in DNA replication, Invitrogen XPF antibody (Lab vision, 219) was used in western blot on human samples at 1:500 (fig 8). Nat Commun (2015) ncbi
mouse monoclonal (219)
  • immunohistochemistry; human; 1:3000; fig s3
In order to characterize unresectable, locally advanced head and neck squamous cell carcinoma in an Easter cooperative oncology group trial E3303 phase II study of cetuximab in combination with radiation and cisplatin, Invitrogen XPF antibody (NeoMarkers, MS-1381-P) was used in immunohistochemistry on human samples at 1:3000 (fig s3). Clin Cancer Res (2014) ncbi
mouse monoclonal (219)
In order to study a novel Fanconi anemia subtype in which SLX4 is mutated, Invitrogen XPF antibody (Thermo, MS-1381) was used . Nat Genet (2011) ncbi
mouse monoclonal (51)
  • immunocytochemistry; human; 1:100
In order to study the repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells, Invitrogen XPF antibody (Neomarkers, ms-1385-P1ABX) was used in immunocytochemistry on human samples at 1:100. J Biol Chem (2009) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 1
Bethyl XPF antibody (Bethyl/Euromedex, A301- 315A) was used in western blot on human samples (fig 1). Cell (2014) ncbi
Articles Reviewed
  1. Sabatella M, Theil A, Ribeiro Silva C, Slyskova J, Thijssen K, Voskamp C, et al. Repair protein persistence at DNA lesions characterizes XPF defect with Cockayne syndrome features. Nucleic Acids Res. 2018;46:9563-9577 pubmed publisher
  2. Slyskova J, Sabatella M, Ribeiro Silva C, Stok C, Theil A, Vermeulen W, et al. Base and nucleotide excision repair facilitate resolution of platinum drugs-induced transcription blockage. Nucleic Acids Res. 2018;46:9537-9549 pubmed publisher
  3. Galanos P, Pappas G, Polyzos A, Kotsinas A, Svolaki I, Giakoumakis N, et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol. 2018;19:37 pubmed publisher
  4. van Cuijk L, van Belle G, Turkyilmaz Y, Poulsen S, Janssens R, Theil A, et al. SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair. Nat Commun. 2015;6:7499 pubmed publisher
  5. Fu H, Martin M, Regairaz M, Huang L, You Y, Lin C, et al. The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage. Nat Commun. 2015;6:6746 pubmed publisher
  6. Egloff A, Lee J, Langer C, Quon H, Vaezi A, Grandis J, et al. Phase II study of cetuximab in combination with cisplatin and radiation in unresectable, locally advanced head and neck squamous cell carcinoma: Eastern cooperative oncology group trial E3303. Clin Cancer Res. 2014;20:5041-51 pubmed publisher
  7. Laguette N, Brégnard C, Hue P, Basbous J, Yatim A, Larroque M, et al. Premature activation of the SLX4 complex by Vpr promotes G2/M arrest and escape from innate immune sensing. Cell. 2014;156:134-45 pubmed publisher
  8. Stoepker C, Hain K, Schuster B, Hilhorst Hofstee Y, Rooimans M, Steltenpool J, et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet. 2011;43:138-41 pubmed publisher
  9. Muniandy P, Thapa D, Thazhathveetil A, Liu S, Seidman M. Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells. J Biol Chem. 2009;284:27908-17 pubmed publisher