This is a Validated Antibody Database (VAD) review about human WWP1, based on 5 published articles (read how Labome selects the articles), using WWP1 antibody in all methods. It is aimed to help Labome visitors find the most suited WWP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WWP1 synonym: AIP5; Tiul1; hSDRP1

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunohistochemistry; rhesus macaque; 1:100; loading ...; fig 1f
  • western blot; rhesus macaque; 1:1000; loading ...; fig 1d
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 6a
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1c
Abcam WWP1 antibody (Abcam, ab43791) was used in immunohistochemistry on rhesus macaque samples at 1:100 (fig 1f), in western blot on rhesus macaque samples at 1:1000 (fig 1d), in western blot knockout validation on mouse samples at 1:1000 (fig 6a) and in immunohistochemistry on mouse samples at 1:100 (fig 1c). Front Cell Dev Biol (2021) ncbi
Abnova
mouse monoclonal (1A7)
  • western blot; human; 1:1000; fig 2
Abnova WWP1 antibody (Abnova, H00011059-M01) was used in western blot on human samples at 1:1000 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (1A7)
  • immunohistochemistry - paraffin section; human; 1:1000
In order to test if Wwp1 regulates connexin 43, Abnova WWP1 antibody (Abnova, 1A7) was used in immunohistochemistry - paraffin section on human samples at 1:1000. J Mol Cell Cardiol (2015) ncbi
mouse monoclonal (1A7)
  • western blot; human; fig 4
Abnova WWP1 antibody (Abnova, H00011059-M01) was used in western blot on human samples (fig 4). Oncotarget (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry; rhesus macaque; 1:100; loading ...; fig 1f
  • western blot; rhesus macaque; 1:1000; loading ...; fig 1d
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 6a
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1c
Abcam WWP1 antibody (Abcam, ab43791) was used in immunohistochemistry on rhesus macaque samples at 1:100 (fig 1f), in western blot on rhesus macaque samples at 1:1000 (fig 1d), in western blot knockout validation on mouse samples at 1:1000 (fig 6a) and in immunohistochemistry on mouse samples at 1:100 (fig 1c). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1h
  • western blot; mouse; 1:1000; loading ...; fig 1g
Abcam WWP1 antibody (Abcam, ab43791) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1h) and in western blot on mouse samples at 1:1000 (fig 1g). Circulation (2021) ncbi
Articles Reviewed
  1. Zhong G, Zhao D, Li J, Liu Z, Pan J, Yuan X, et al. WWP1 Deficiency Alleviates Cardiac Remodeling Induced by Simulated Microgravity. Front Cell Dev Biol. 2021;9:739944 pubmed publisher
  2. Zhao D, Zhong G, Li J, Pan J, Zhao Y, Song H, et al. Targeting E3 Ubiquitin-Ligase WWP1 Prevents Cardiac Hypertrophy Through Destabilizing DVL2 via Inhibition of K27-Linked Ubiquitination. Circulation. 2021;: pubmed publisher
  3. Qin J, Zhou Z, Chen W, Wang C, Zhang H, Ge G, et al. BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5. Nat Commun. 2015;6:8471 pubmed publisher
  4. Basheer W, Harris B, Mentrup H, Abreha M, Thames E, Lea J, et al. Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis. J Mol Cell Cardiol. 2015;88:1-13 pubmed publisher
  5. Ge F, Chen W, Qin J, Zhou Z, Liu R, Liu L, et al. Ataxin-3 like (ATXN3L), a member of the Josephin family of deubiquitinating enzymes, promotes breast cancer proliferation by deubiquitinating Krüppel-like factor 5 (KLF5). Oncotarget. 2015;6:21369-78 pubmed