This is a Validated Antibody Database (VAD) review about human WRN, based on 9 published articles (read how Labome selects the articles), using WRN antibody in all methods. It is aimed to help Labome visitors find the most suited WRN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WRN synonym: RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; DNA helicase, RecQ-like type 3; Werner syndrome, RecQ helicase-like; exonuclease WRN; recQ protein-like 2

Novus Biologicals
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 1c
  • western blot; human; loading ...; fig 3c
Novus Biologicals WRN antibody (Novusbio, NB100-471) was used in immunoprecipitation on human samples (fig 1c) and in western blot on human samples (fig 3c). Oncogene (2019) ncbi
rabbit polyclonal
  • reverse phase protein lysate microarray; human; 1:1000; fig 5
In order to identify a novel DNA2- and WRN-dependent mechanism of reversed replication fork processing and restart after prolonged genotoxic stress, Novus Biologicals WRN antibody (Novus, NB100-471) was used in reverse phase protein lysate microarray on human samples at 1:1000 (fig 5). J Cell Biol (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Abcam WRN antibody (Abcam, ab200) was used in western blot on human samples (fig 2a). Genes Dev (2017) ncbi
MilliporeSigma
mouse monoclonal (195C)
  • western blot; human; loading ...; fig 2c
MilliporeSigma WRN antibody (Sigma, W0393) was used in western blot on human samples (fig 2c). Indian J Gastroenterol (2017) ncbi
mouse monoclonal (195C)
  • western blot; human; loading ...; fig 3f
In order to propose that WRN interacts with HDACs to facilitate activity of stalled replication forks under conditions of replication stress, MilliporeSigma WRN antibody (Sigma, W0393) was used in western blot on human samples (fig 3f). J Biol Chem (2016) ncbi
mouse monoclonal (195C)
  • western blot; human; loading ...; fig 2a
MilliporeSigma WRN antibody (Sigma, W0393) was used in western blot on human samples (fig 2a). Clin Cancer Res (2016) ncbi
Cell Signaling Technology
mouse monoclonal (8H3)
  • western blot; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology WRN antibody (Cell Signaling, 4666) was used in western blot on human samples at 1:1000 (fig 2a). elife (2019) ncbi
mouse monoclonal (8H3)
  • western blot; human; loading ...; fig 2s4
In order to investigate DNA repair in CD44+/CD24- cells, Cell Signaling Technology WRN antibody (cell signalling, 4666) was used in western blot on human samples (fig 2s4). elife (2017) ncbi
mouse monoclonal (8H3)
  • western blot; human; loading ...; fig 3a
In order to study quinacrine-induced apoptosis, Cell Signaling Technology WRN antibody (Cell Signaling, 4666) was used in western blot on human samples (fig 3a). Biochem Pharmacol (2016) ncbi
Articles Reviewed
  1. Lieb S, Blaha Ostermann S, Kamper E, Rippka J, Schwarz C, Ehrenhöfer Wölfer K, et al. Werner syndrome helicase is a selective vulnerability of microsatellite instability-high tumor cells. elife. 2019;8: pubmed publisher
  2. Liu B, Yi J, Yang X, Liu L, Lou X, Zhang Z, et al. MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner. Oncogene. 2019;38:2501-2515 pubmed publisher
  3. Amalnath S, Sargolzaeiaval F, Oshima J, Baskar D. Uncommon cause of cirrhosis-A case of Werner syndrome with a novel WRN mutation. Indian J Gastroenterol. 2017;36:323-325 pubmed publisher
  4. Gong Y, Handa N, Kowalczykowski S, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev. 2017;31:46-58 pubmed publisher
  5. Pal D, Pertot A, Shirole N, Yao Z, Anaparthy N, Garvin T, et al. TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells. elife. 2017;6: pubmed publisher
  6. Kehrli K, Phelps M, Lazarchuk P, Chen E, Monnat R, Sidorova J. Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest. J Biol Chem. 2016;291:24487-24503 pubmed
  7. Bosch L, Luo Y, Lao V, Snaebjornsson P, Trooskens G, Vlassenbroeck I, et al. WRN Promoter CpG Island Hypermethylation Does Not Predict More Favorable Outcomes for Patients with Metastatic Colorectal Cancer Treated with Irinotecan-Based Therapy. Clin Cancer Res. 2016;22:4612-22 pubmed publisher
  8. Preet R, Siddharth S, Satapathy S, Das S, Nayak A, Das D, et al. Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade. Biochem Pharmacol. 2016;105:23-33 pubmed publisher
  9. Thangavel S, Berti M, Levikova M, Pinto C, Gomathinayagam S, Vujanovic M, et al. DNA2 drives processing and restart of reversed replication forks in human cells. J Cell Biol. 2015;208:545-62 pubmed publisher