This is a Validated Antibody Database (VAD) review about human WNT5A, based on 18 published articles (read how Labome selects the articles), using WNT5A antibody in all methods. It is aimed to help Labome visitors find the most suited WNT5A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WNT5A synonym: hWNT5A

Knockout validation
R&D Systems
rat monoclonal (442625)
  • western blot knockout validation; human; 1:300; loading ...; fig 1c
R&D Systems WNT5A antibody (R&D Systems, MAB645) was used in western blot knockout validation on human samples at 1:300 (fig 1c). Oncogene (2018) ncbi
R&D Systems
rat monoclonal (442625)
  • western blot knockout validation; human; 1:300; loading ...; fig 1c
R&D Systems WNT5A antibody (R&D Systems, MAB645) was used in western blot knockout validation on human samples at 1:300 (fig 1c). Oncogene (2018) ncbi
rat monoclonal (442625)
  • western blot; mouse; loading ...; fig 1a
  • western blot; human; loading ...; fig 2d, 3f
R&D Systems WNT5A antibody (R&D, MAB645) was used in western blot on mouse samples (fig 1a) and in western blot on human samples (fig 2d, 3f). J Exp Med (2017) ncbi
rat monoclonal (442625)
  • immunohistochemistry - frozen section; mouse; fig 1
R&D Systems WNT5A antibody (R&D Systems, MAB645) was used in immunohistochemistry - frozen section on mouse samples (fig 1). Dev Biol (2016) ncbi
rat monoclonal (442625)
  • western blot; human; fig 1
R&D Systems WNT5A antibody (R&D, MAB645) was used in western blot on human samples (fig 1). Clin Exp Metastasis (2016) ncbi
rat monoclonal (442625)
  • western blot; mouse; 1:250; fig 3
In order to assess MTV-Wnt1 tumors and how Wnt5a suppresses tumor formation and redirects tumor phenotype, R&D Systems WNT5A antibody (R&D Systems, MAB645) was used in western blot on mouse samples at 1:250 (fig 3). PLoS ONE (2014) ncbi
rat monoclonal (442625)
  • western blot; human; 1:5000; fig 2a
R&D Systems WNT5A antibody (R&D, Mab-645) was used in western blot on human samples at 1:5000 (fig 2a). Dev Biol (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-5)
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig 4c
Santa Cruz Biotechnology WNT5A antibody (Santa Cruz, sc-365370) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig 4c). Nat Neurosci (2019) ncbi
mouse monoclonal (A-5)
  • western blot; human; fig s1
Santa Cruz Biotechnology WNT5A antibody (Santa Cruz, sc-365370) was used in western blot on human samples (fig s1). Int J Mol Sci (2016) ncbi
mouse monoclonal (A-5)
  • western blot; human; fig 2
Santa Cruz Biotechnology WNT5A antibody (santa Cruz, sc-365370) was used in western blot on human samples (fig 2). BMC Res Notes (2015) ncbi
mouse monoclonal (A-5)
  • western blot; mouse; fig 4a
Santa Cruz Biotechnology WNT5A antibody (Santa Cruz Biotechnology, sc-365370) was used in western blot on mouse samples (fig 4a). PLoS ONE (2015) ncbi
mouse monoclonal (A-5)
  • western blot; mouse
Santa Cruz Biotechnology WNT5A antibody (Santa Cruz, sc-365370) was used in western blot on mouse samples . Exp Cell Res (2014) ncbi
LifeSpan Biosciences
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; human; 1:300; fig 1
  • ELISA; human
LifeSpan Biosciences WNT5A antibody (LSBio, 3A4) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 1) and in ELISA on human samples . Sci Rep (2016) ncbi
MilliporeSigma
mouse monoclonal (3A4)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig s1a
  • western blot; human; fig s3a
In order to discuss the impact of Wnt ligands on aortic valve calcification, MilliporeSigma WNT5A antibody (Sigma, SAB1402393) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig s1a) and in western blot on human samples (fig s3a). Arterioscler Thromb Vasc Biol (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C27E8)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology WNT5A antibody (CST, 2530) was used in western blot on mouse samples at 1:1000 (fig 4b). Sci Adv (2019) ncbi
domestic rabbit monoclonal (C27E8)
  • western blot; human; 1:1000; fig 3a
In order to investigate the effects of let-7 family of microRNAs on triple negative breast cancer cells, Cell Signaling Technology WNT5A antibody (Cell Signaling, 2530) was used in western blot on human samples at 1:1000 (fig 3a). Mol Med Rep (2016) ncbi
domestic rabbit monoclonal (C27E8)
  • western blot; mouse; 1:1000; fig 6
In order to assess the effects of Shenling Baizhu San on colitis-associated colorectal cancer, Cell Signaling Technology WNT5A antibody (CST, C27E8) was used in western blot on mouse samples at 1:1000 (fig 6). BMC Complement Altern Med (2015) ncbi
domestic rabbit monoclonal (C27E8)
  • western blot; human
Cell Signaling Technology WNT5A antibody (Cell Signaling, 2530) was used in western blot on human samples . Cell Tissue Res (2014) ncbi
domestic rabbit monoclonal (C27E8)
  • western blot; human; 1:1000
Cell Signaling Technology WNT5A antibody (Cell Signaling, C27E8) was used in western blot on human samples at 1:1000. BMC Cell Biol (2013) ncbi
Articles Reviewed
  1. van de Vlekkert D, Demmers J, Nguyen X, Campos Y, Machado E, Annunziata I, et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis. Sci Adv. 2019;5:eaav3270 pubmed publisher
  2. Giandomenico S, Mierau S, Gibbons G, Wenger L, Masullo L, Sit T, et al. Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output. Nat Neurosci. 2019;22:669-679 pubmed publisher
  3. Han B, Zhou B, Qu Y, Gao B, Xu Y, Chung S, et al. FOXC1-induced non-canonical WNT5A-MMP7 signaling regulates invasiveness in triple-negative breast cancer. Oncogene. 2018;37:1399-1408 pubmed publisher
  4. Baarsma H, Skronska Wasek W, Mutze K, Ciolek F, Wagner D, John Schuster G, et al. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD. J Exp Med. 2017;214:143-163 pubmed publisher
  5. Albanese I, Yu B, Al Kindi H, Barratt B, Ott L, Al Refai M, et al. Role of Noncanonical Wnt Signaling Pathway in Human Aortic Valve Calcification. Arterioscler Thromb Vasc Biol. 2017;37:543-552 pubmed publisher
  6. Sun H, Ding C, Zhang H, Gao J. Let?7 miRNAs sensitize breast cancer stem cells to radiation?induced repression through inhibition of the cyclin D1/Akt1/Wnt1 signaling pathway. Mol Med Rep. 2016;14:3285-92 pubmed publisher
  7. Meinhardt G, Saleh L, Otti G, Haider S, Velicky P, Fiala C, et al. Wingless ligand 5a is a critical regulator of placental growth and survival. Sci Rep. 2016;6:28127 pubmed publisher
  8. Li D, Sinha T, Ajima R, Seo H, Yamaguchi T, Wang J. Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment. Dev Biol. 2016;412:18-31 pubmed publisher
  9. Bleckmann A, Conradi L, Menck K, Schmick N, Schubert A, Rietkötter E, et al. β-catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases. Clin Exp Metastasis. 2016;33:309-23 pubmed publisher
  10. Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, et al. Wnt16 Signaling Is Required for IL-1β-Induced Matrix Metalloproteinase-13-Regulated Proliferation of Human Stem Cell-Derived Osteoblastic Cells. Int J Mol Sci. 2016;17:221 pubmed publisher
  11. Herzog J, Rid R, Wagner M, Hundsberger H, Eger A, Bauer J, et al. Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways. BMC Res Notes. 2015;8:785 pubmed publisher
  12. Lin X, Xu W, Shao M, Fan Q, Wen G, Li C, et al. Shenling Baizhu San supresses colitis associated colorectal cancer through inhibition of epithelial-mesenchymal transition and myeloid-derived suppressor infiltration. BMC Complement Altern Med. 2015;15:126 pubmed publisher
  13. Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, et al. Interleukin-1β-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells. PLoS ONE. 2015;10:e0124542 pubmed publisher
  14. Easter S, Mitchell E, Baxley S, Desmond R, Frost A, Serra R. Wnt5a suppresses tumor formation and redirects tumor phenotype in MMTV-Wnt1 tumors. PLoS ONE. 2014;9:e113247 pubmed publisher
  15. Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, et al. IL-1?-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway. Exp Cell Res. 2014;328:69-86 pubmed publisher
  16. Pei M, Li J, Zhang Y, Liu G, Wei L, Zhang Y. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res. 2014;356:391-403 pubmed publisher
  17. Ogunkolade B, Jones T, Aarum J, Szary J, Owen N, Ottaviani D, et al. BORIS/CTCFL is an RNA-binding protein that associates with polysomes. BMC Cell Biol. 2013;14:52 pubmed publisher
  18. Buttler K, Becker J, Pukrop T, Wilting J. Maldevelopment of dermal lymphatics in Wnt5a-knockout-mice. Dev Biol. 2013;381:365-76 pubmed publisher