This is a Validated Antibody Database (VAD) review about human WNT3A, based on 21 published articles (read how Labome selects the articles), using WNT3A antibody in all methods. It is aimed to help Labome visitors find the most suited WNT3A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Abcam
domestic rabbit monoclonal
  • western blot; rat; 1:1000; loading ...; fig 2j
Abcam WNT3A antibody (Abcam, ab219412) was used in western blot on rat samples at 1:1000 (fig 2j). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal
  • western blot; rat; 1:1000; loading ...; fig 7b
Abcam WNT3A antibody (Abcam, ab219412) was used in western blot on rat samples at 1:1000 (fig 7b). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (EPR21889)
  • western blot; human; loading ...; fig 5e, 5f
Abcam WNT3A antibody (Abcam, ab234099) was used in western blot on human samples (fig 5e, 5f). Aging (Albany NY) (2021) ncbi
mouse monoclonal (3A6)
  • western blot; human; 1:1000; fig 6f
Abcam WNT3A antibody (Abcam, ab81614) was used in western blot on human samples at 1:1000 (fig 6f). Nat Commun (2021) ncbi
domestic rabbit monoclonal
  • western blot; human; loading ...; fig 6a
Abcam WNT3A antibody (Abcam, ab219412) was used in western blot on human samples (fig 6a). Braz J Med Biol Res (2019) ncbi
mouse monoclonal (3A6)
  • western blot; human; loading ...; fig 4b
Abcam WNT3A antibody (Abcam, ab81614) was used in western blot on human samples (fig 4b). Cell Commun Signal (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 4b
Abcam WNT3A antibody (Abcam, ab28472) was used in western blot on mouse samples at 1:500 (fig 4b). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 6b
Abcam WNT3A antibody (Abcam, ab28472) was used in western blot on rat samples (fig 6b). Braz J Med Biol Res (2019) ncbi
domestic rabbit monoclonal
  • western blot; human; 1:3000; fig 3b
Abcam WNT3A antibody (Abcam, ab219412) was used in western blot on human samples at 1:3000 (fig 3b). Mol Med Rep (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig 7i
Abcam WNT3A antibody (Abcam, ab19925) was used in immunohistochemistry on mouse samples at 1:50 (fig 7i). Science (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3a
Abcam WNT3A antibody (Abcam, ab28472) was used in western blot on human samples (fig 3a). EMBO J (2018) ncbi
mouse monoclonal (3A6)
  • western blot; human; 1:2000; loading ...; fig 1c
Abcam WNT3A antibody (Abcam, ab81614) was used in western blot on human samples at 1:2000 (fig 1c). Biosci Rep (2018) ncbi
R&D Systems
rat monoclonal (930769)
  • flow cytometry; mouse
R&D Systems WNT3A antibody (R&D Systems, MAB9025) was used in flow cytometry on mouse samples . elife (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-9)
  • western blot; mouse; 1:1000; loading ...; fig 9b
Santa Cruz Biotechnology WNT3A antibody (Santa Cruz Biotechnology, sc-74537) was used in western blot on mouse samples at 1:1000 (fig 9b). Cell Mol Gastroenterol Hepatol (2020) ncbi
mouse monoclonal (3A6)
  • western blot; human; 1:200; fig 3
In order to assess the promotion of wnt3a stabilization through suppression of miR-128 by AurkA that controls self-renewal of breast cancer-initiating cells, Santa Cruz Biotechnology WNT3A antibody (Santa Cruz, sc-136163) was used in western blot on human samples at 1:200 (fig 3). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C64F2)
  • immunocytochemistry; human; 1:200; loading ...; fig 6e
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology WNT3A antibody (Cell Signaling Technology, 2721) was used in immunocytochemistry on human samples at 1:200 (fig 6e) and in western blot on human samples at 1:1000. Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (C64F2)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology WNT3A antibody (Cell signaling, 2721) was used in western blot on human samples (fig 5a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (C64F2)
  • western blot; human; loading ...; fig 5a
In order to describe the dual roles of TbetaRIII/betaglycan, Cell Signaling Technology WNT3A antibody (Cell signaling, C64F2) was used in western blot on human samples (fig 5a). J Biol Chem (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:50; fig 3
  • western blot; rat; 1:50; fig 2
Cell Signaling Technology WNT3A antibody (Cell Signaling Technology, 2391S) was used in immunohistochemistry - paraffin section on rat samples at 1:50 (fig 3) and in western blot on rat samples at 1:50 (fig 2). Mol Med Rep (2016) ncbi
domestic rabbit monoclonal (C64F2)
  • western blot; human
Cell Signaling Technology WNT3A antibody (Cell Signaling, 2721) was used in western blot on human samples . Cell Tissue Res (2014) ncbi
domestic rabbit monoclonal (C64F2)
  • western blot; human; 1:1000
Cell Signaling Technology WNT3A antibody (Cell Signaling, C64F2) was used in western blot on human samples at 1:1000. BMC Cell Biol (2013) ncbi
Articles Reviewed
  1. Han H, Tian T, Huang G, Li D, Yang S. The lncRNA H19/miR-541-3p/Wnt/β-catenin axis plays a vital role in melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells. Aging (Albany NY). 2021;13:18257-18273 pubmed publisher
  2. Sha S, Shen X, Cao Y, Qu L. Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer's disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway. Aging (Albany NY). 2021;13:15285-15306 pubmed publisher
  3. Yao J, Yang Z, Yang J, Wang Z, Zhang Z. Long non-coding RNA FEZF1-AS1 promotes the proliferation and metastasis of hepatocellular carcinoma via targeting miR-107/Wnt/β-catenin axis. Aging (Albany NY). 2021;13:13726-13738 pubmed publisher
  4. Weinstock A, RAHMAN K, Yaacov O, Nishi H, Menon P, Nikain C, et al. Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis. elife. 2021;10: pubmed publisher
  5. Xu J, Deng X, Wu X, Zhu H, Zhu Y, Liu J, et al. Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway. Aging (Albany NY). 2021;13:8989-9010 pubmed publisher
  6. Wu C, Dicks A, Steward N, Tang R, Katz D, Choi Y, et al. Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nat Commun. 2021;12:362 pubmed publisher
  7. Xing T, Benderman L, Sabu S, Parker J, Yang J, Lu Q, et al. Tight Junction Protein Claudin-7 Is Essential for Intestinal Epithelial Stem Cell Self-Renewal and Differentiation. Cell Mol Gastroenterol Hepatol. 2020;9:641-659 pubmed publisher
  8. Yang X, Jiang J, Zhang C, Li Y. Baicalein restrains proliferation, migration, and invasion of human malignant melanoma cells by down-regulating colon cancer associated transcript-1. Braz J Med Biol Res. 2019;52:e8934 pubmed publisher
  9. Jiang S, Zhang M, Zhang Y, Zhou W, Zhu T, Ruan Q, et al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal. 2019;17:109 pubmed publisher
  10. van de Vlekkert D, Demmers J, Nguyen X, Campos Y, Machado E, Annunziata I, et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis. Sci Adv. 2019;5:eaav3270 pubmed publisher
  11. Zhang X, Qin Q, Dai H, Cai S, Zhou C, Guan J. Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression. Braz J Med Biol Res. 2019;52:e7994 pubmed publisher
  12. Zhang C, Wang Y. Metformin attenuates cells stemness and epithelial‑mesenchymal transition in colorectal cancer cells by inhibiting the Wnt3a/β‑catenin pathway. Mol Med Rep. 2019;19:1203-1209 pubmed publisher
  13. Chakrabarti R, Celià Terrassa T, Kumar S, Hang X, Wei Y, Choudhury A, et al. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science. 2018;360: pubmed publisher
  14. Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  15. Xue C, Hong L, Lin J, Yao X, Wu D, Lin X, et al. β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway. Biosci Rep. 2018;38: pubmed publisher
  16. Tüysüz N, van Bloois L, van den Brink S, Begthel H, Verstegen M, Cruz L, et al. Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells. Nat Commun. 2017;8:14578 pubmed publisher
  17. JENKINS L, Singh P, Varadaraj A, Lee N, Shah S, Flores H, et al. Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling. J Biol Chem. 2016;291:25716-25728 pubmed
  18. Eterno V, Zambelli A, Villani L, Tuscano A, Manera S, Spitaleri A, et al. AurkA controls self-renewal of breast cancer-initiating cells promoting wnt3a stabilization through suppression of miR-128. Sci Rep. 2016;6:28436 pubmed publisher
  19. Liu S, Zhou P, Zhang Y. Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis. Mol Med Rep. 2016;13:1999-2006 pubmed publisher
  20. Pei M, Li J, Zhang Y, Liu G, Wei L, Zhang Y. Expansion on a matrix deposited by nonchondrogenic urine stem cells strengthens the chondrogenic capacity of repeated-passage bone marrow stromal cells. Cell Tissue Res. 2014;356:391-403 pubmed publisher
  21. Ogunkolade B, Jones T, Aarum J, Szary J, Owen N, Ottaviani D, et al. BORIS/CTCFL is an RNA-binding protein that associates with polysomes. BMC Cell Biol. 2013;14:52 pubmed publisher