This is a Validated Antibody Database (VAD) review about human WEE1, based on 27 published articles (read how Labome selects the articles), using WEE1 antibody in all methods. It is aimed to help Labome visitors find the most suited WEE1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WEE1 synonym: WEE1A; WEE1hu

Santa Cruz Biotechnology
mouse monoclonal (B-11)
  • western blot; human; 1:200; loading ...; fig 3a, 3b, 3c
Santa Cruz Biotechnology WEE1 antibody (Santa Cruz, sc-5285) was used in western blot on human samples at 1:200 (fig 3a, 3b, 3c). Ther Adv Hematol (2020) ncbi
mouse monoclonal (B-11)
  • western blot; human; fig 3
Santa Cruz Biotechnology WEE1 antibody (Santa Cruz, sc-5285) was used in western blot on human samples (fig 3). BMC Cancer (2016) ncbi
mouse monoclonal (B-11)
  • immunohistochemistry - paraffin section; human; loading ...; fig 6d
  • western blot; human; 1:1000
Santa Cruz Biotechnology WEE1 antibody (SantaCruz, B-11) was used in immunohistochemistry - paraffin section on human samples (fig 6d) and in western blot on human samples at 1:1000. Oncotarget (2016) ncbi
mouse monoclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 6d
  • western blot; human; 1:1000
Santa Cruz Biotechnology WEE1 antibody (SantaCruz, B-11) was used in immunohistochemistry - paraffin section on human samples (fig 6d) and in western blot on human samples at 1:1000. Oncotarget (2016) ncbi
mouse monoclonal (B-11)
  • western blot; human; 1:1000; fig 6
Santa Cruz Biotechnology WEE1 antibody (Santa Cruz, sc-5285) was used in western blot on human samples at 1:1000 (fig 6). Oncotarget (2016) ncbi
mouse monoclonal (B-11)
  • immunohistochemistry; mouse; 1:50
In order to study the role of Cdk1 signaling in the mechanism by which filamin B modulates the proliferation and differentiation of chondrocytes, Santa Cruz Biotechnology WEE1 antibody (Santa Cruz, sc-5285) was used in immunohistochemistry on mouse samples at 1:50. PLoS ONE (2014) ncbi
mouse monoclonal (B-11)
  • western blot; human; 1:200
In order to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism, Santa Cruz Biotechnology WEE1 antibody (Santa Cruz Biotechnology, sc-5285) was used in western blot on human samples at 1:200. Virology (2013) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; fig 5c
Abcam WEE1 antibody (Abcam, ab111820) was used in western blot on human samples (fig 5c). JCI Insight (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 1
Abcam WEE1 antibody (Abcam, ab137377) was used in western blot on mouse samples (fig 1). Cell Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D47G5)
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology WEE1 antibody (Cell Signaling, 4910) was used in western blot on human samples (fig 2c). Mol Ther Oncolytics (2022) ncbi
domestic rabbit monoclonal (D47G5)
  • western blot; human; 1:1000; loading ...; fig 1d
  • western blot; mouse; 1:1000; fig 1f
Cell Signaling Technology WEE1 antibody (Cell Signaling, 4910) was used in western blot on human samples at 1:1000 (fig 1d) and in western blot on mouse samples at 1:1000 (fig 1f). Cell Rep (2022) ncbi
domestic rabbit monoclonal (D10D2)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1e
  • western blot; human; 1:1000; loading ...; fig 2c, 2d, 2e
Cell Signaling Technology WEE1 antibody (CST, 13084) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1e) and in western blot on human samples at 1:1000 (fig 2c, 2d, 2e). J Exp Clin Cancer Res (2021) ncbi
domestic rabbit monoclonal (D10D2)
  • immunohistochemistry; human; 1:200; loading ...; fig 4i
  • western blot; mouse; 1:1000; loading ...; fig 3c
Cell Signaling Technology WEE1 antibody (Cell Signaling Technology, 13084) was used in immunohistochemistry on human samples at 1:200 (fig 4i) and in western blot on mouse samples at 1:1000 (fig 3c). J Biol Chem (2021) ncbi
domestic rabbit monoclonal (D47G5)
  • western blot; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology WEE1 antibody (Cell Signaling Technology, 4910) was used in western blot on human samples at 1:1000 (fig 3b). Clin Transl Med (2021) ncbi
domestic rabbit monoclonal (D10D2)
  • western blot; human; 1:1000; loading ...; fig s5c
Cell Signaling Technology WEE1 antibody (Cell Signaling, D10D2) was used in western blot on human samples at 1:1000 (fig s5c). Leukemia (2021) ncbi
domestic rabbit monoclonal (D10D2)
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology WEE1 antibody (CST, 13084) was used in western blot on human samples (fig 3d). Mol Cell Biol (2020) ncbi
domestic rabbit monoclonal (D10D2)
  • western blot; human; loading ...; fig 5e, s3b
Cell Signaling Technology WEE1 antibody (Cell Signaling, 13084) was used in western blot on human samples (fig 5e, s3b). Cell Death Dis (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology WEE1 antibody (Cell Signaling Technology, 4936) was used in western blot on human samples (fig 3c). Cells (2019) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology WEE1 antibody (Cell Signaling, 4936) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (D47G5)
  • other; human; loading ...; fig 4c
Cell Signaling Technology WEE1 antibody (Cell Signaling, 4910) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3g
Cell Signaling Technology WEE1 antibody (cell signalling, 4936) was used in western blot on human samples at 1:1000 (fig 3g). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2e
In order to identify and characterize an inhibitor of the COP9 signalosome, Cell Signaling Technology WEE1 antibody (Cell Signaling, 4936) was used in western blot on human samples at 1:1000 (fig 2e). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2c
In order to elucidate the upstream mechanisms of apoptosis triggered by an anti-microtubule drug, ABT-751, Cell Signaling Technology WEE1 antibody (cell signalling, 4936) was used in western blot on human samples at 1:1000 (fig 2c). Toxicol Appl Pharmacol (2016) ncbi
domestic rabbit monoclonal (D47G5)
  • western blot; human; fig 5
Cell Signaling Technology WEE1 antibody (Cell signaling, 4910) was used in western blot on human samples (fig 5). J Biol Chem (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology WEE1 antibody (Cell signaling, 4936s) was used in western blot on human samples (fig 1). EMBO Mol Med (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1c
In order to elucidate the roles of hRpn13 and Uch37 in cell cycle progression, Cell Signaling Technology WEE1 antibody (Cell Signaling, 4936) was used in western blot on human samples (fig 1c). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D10D2)
  • western blot; human; 1:1000; fig 4
Cell Signaling Technology WEE1 antibody (Cell Signaling, D10D2) was used in western blot on human samples at 1:1000 (fig 4). Cell Rep (2015) ncbi
domestic rabbit monoclonal (D47G5)
  • western blot; human; 1:1000
Cell Signaling Technology WEE1 antibody (Cell Signaling Technology, 4910) was used in western blot on human samples at 1:1000. Cancer Lett (2015) ncbi
domestic rabbit monoclonal (D47G5)
  • western blot; human
Cell Signaling Technology WEE1 antibody (Cell Signaling, 4910) was used in western blot on human samples . Oncogene (2013) ncbi
Articles Reviewed
  1. Fei X, Wu X, Dou Y, Sun K, Guo Q, Zhang L, et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics. 2022;26:413-428 pubmed publisher
  2. Taniguchi H, Caeser R, Chavan S, Zhan Y, Chow A, Manoj P, et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022;39:110814 pubmed publisher
  3. Li C, Shen Q, Zhang P, Wang T, Liu W, Li R, et al. Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells. J Exp Clin Cancer Res. 2021;40:315 pubmed publisher
  4. Jokoji G, Maeda S, Oishi K, Ijuin T, Nakajima M, Tawaratsumida H, et al. CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. J Biol Chem. 2021;297:100994 pubmed publisher
  5. Sakai H, Kawakami H, Teramura T, Onodera Y, Somers E, Furuuchi K, et al. Folate receptor α increases chemotherapy resistance through stabilizing MDM2 in cooperation with PHB2 that is overcome by MORAb-202 in gastric cancer. Clin Transl Med. 2021;11:e454 pubmed publisher
  6. Grandits A, Nguyen C, Schlerka A, Hackl H, Sill H, Etzler J, et al. Downregulation of MTSS1 in acute myeloid leukemia is associated with a poor prognosis, chemotherapy resistance, and disease aggressiveness. Leukemia. 2021;35:2827-2839 pubmed publisher
  7. Ye S, Sharipova D, Kozinova M, Klug L, D Souza J, Belinsky M, et al. Identification of Wee1 as a target in combination with avapritinib for gastrointestinal stromal tumor treatment. JCI Insight. 2021;6: pubmed publisher
  8. Osei Amponsa V, Sridharan V, Tandon M, Evans C, Klarmann K, Cheng K, et al. Impact of losing hRpn13 Pru or UCHL5 on proteasome clearance of ubiquitinated proteins and RA190 cytotoxicity. Mol Cell Biol. 2020;: pubmed publisher
  9. de Jong M, Langendonk M, Reitsma B, Herbers P, Lodewijk M, Nijland M, et al. WEE1 inhibition synergizes with CHOP chemotherapy and radiation therapy through induction of premature mitotic entry and DNA damage in diffuse large B-cell lymphoma. Ther Adv Hematol. 2020;11:2040620719898373 pubmed publisher
  10. Liu G, Zhang Q, Xia L, Shi M, Cai J, Zhang H, et al. RNA-binding protein CELF6 is cell cycle regulated and controls cancer cell proliferation by stabilizing p21. Cell Death Dis. 2019;10:688 pubmed publisher
  11. Lee J, Sung J, Choi E, Yoon H, Kang B, Hong E, et al. C/EBPβ Is a Transcriptional Regulator of Wee1 at the G₂/M Phase of the Cell Cycle. Cells. 2019;8: pubmed publisher
  12. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  13. Xu J, Zhou W, Yang F, Chen G, Li H, Zhao Y, et al. The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor. Nat Commun. 2017;8:14002 pubmed publisher
  14. Schlierf A, Altmann E, Quancard J, Jefferson A, Assenberg R, Renatus M, et al. Targeted inhibition of the COP9 signalosome for treatment of cancer. Nat Commun. 2016;7:13166 pubmed publisher
  15. Wei R, Lin S, Wu W, Chen L, Li C, Chen H, et al. A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells. Toxicol Appl Pharmacol. 2016;311:88-98 pubmed publisher
  16. Kim M, Caplen N, Chakka S, Hernandez L, House C, Pongas G, et al. Identification of therapeutic targets applicable to clinical strategies in ovarian cancer. BMC Cancer. 2016;16:678 pubmed publisher
  17. Chen X, Stauffer S, Chen Y, Dong J. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. J Biol Chem. 2016;291:14761-72 pubmed publisher
  18. Al Nakouzi N, Wang C, Beraldi E, Jäger W, Ettinger S, Fazli L, et al. Clusterin knockdown sensitizes prostate cancer cells to taxane by modulating mitosis. EMBO Mol Med. 2016;8:761-78 pubmed publisher
  19. Ho T, Guilbaud G, Blow J, Sale J, Watson C. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Rep. 2016;15:724-734 pubmed publisher
  20. Huang Y, Peng W, Furuuchi N, DuHadaway J, Jimbo M, Pirritano A, et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget. 2016;7:21812-24 pubmed publisher
  21. Randles L, Anchoori R, Roden R, Walters K. The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression. J Biol Chem. 2016;291:8773-83 pubmed publisher
  22. Soriano A, París Coderch L, Jubierre L, Martínez A, Zhou X, Piskareva O, et al. MicroRNA-497 impairs the growth of chemoresistant neuroblastoma cells by targeting cell cycle, survival and vascular permeability genes. Oncotarget. 2016;7:9271-87 pubmed publisher
  23. Toledo C, Ding Y, Hoellerbauer P, Davis R, Basom R, Girard E, et al. Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells. Cell Rep. 2015;13:2425-2439 pubmed publisher
  24. Zanotto Filho A, Braganhol E, Klafke K, Figueiró F, Terra S, Paludo F, et al. Autophagy inhibition improves the efficacy of curcumin/temozolomide combination therapy in glioblastomas. Cancer Lett. 2015;358:220-31 pubmed publisher
  25. Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher
  26. Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  27. Zhang J, Espinoza L, Kinders R, Lawrence S, Pfister T, Zhou M, et al. NANOG modulates stemness in human colorectal cancer. Oncogene. 2013;32:4397-405 pubmed publisher