This is a Validated Antibody Database (VAD) review about human WASHC1, based on 4 published articles (read how Labome selects the articles), using WASHC1 antibody in all methods. It is aimed to help Labome visitors find the most suited WASHC1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WASHC1 synonym: FAM39E; WASH; WASH1; WASH complex subunit 1; CXYorf1-like protein on chromosome 9; FLJ00075 protein; WAS protein family homolog 1; actin nucleation promoting factor; family with sequence similarity 39, member E

Abcam
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s3d
In order to investigate the connection between the centrosome and actin filaments, Abcam WASHC1 antibody (abcam, ab157592) was used in western blot on human samples at 1:1000 (fig s3d). Nat Cell Biol (2016) ncbi
Sigma-Aldrich
monoclonal (WASH1-27)
  • western blot; mouse; loading ...; fig 7f
Sigma-Aldrich WASHC1 antibody (Sigma, SAB-4200552) was used in western blot on mouse samples (fig 7f). Cell Death Dis (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:500
In order to identify a hypomorphic variant of CCDC22 in patients with RSS/3C syndrome in an Austrian family, Sigma-Aldrich WASHC1 antibody (Sigma-Aldrich, SAB4200372) was used in western blot on human samples at 1:500. Eur J Hum Genet (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:300
In order to demonstrate that the Parkinson's disease-causing D620N mutation in VPS35 restricts WASH complex recruitment to endosomes and alters autophagosome formation, Sigma-Aldrich WASHC1 antibody (Sigma, SAB4200372) was used in western blot on human samples at 1:300. Nat Commun (2014) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; human; 1:200
In order to demonstrate that the Parkinson's disease-causing D620N mutation in VPS35 restricts WASH complex recruitment to endosomes and alters autophagosome formation, EMD Millipore WASHC1 antibody (Millipore, ABS72) was used in immunocytochemistry on human samples at 1:200. Nat Commun (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to demonstrate that the Parkinson's disease-causing D620N mutation in VPS35 restricts WASH complex recruitment to endosomes and alters autophagosome formation, EMD Millipore WASHC1 antibody (Millipore, ABS73) was used in western blot on human samples at 1:1000. Nat Commun (2014) ncbi
Articles Reviewed
  1. Su X, Wu C, Ye X, Zeng M, Zhang Z, Che Y, et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis. 2018;9:302 pubmed publisher
  2. Farina F, Gaillard J, Guérin C, Couté Y, Sillibourne J, Blanchoin L, et al. The centrosome is an actin-organizing centre. Nat Cell Biol. 2016;18:65-75 pubmed publisher
  3. Kolanczyk M, Krawitz P, Hecht J, Hupalowska A, Miaczynska M, Marschner K, et al. Missense variant in CCDC22 causes X-linked recessive intellectual disability with features of Ritscher-Schinzel/3C syndrome. Eur J Hum Genet. 2015;23:633-8 pubmed publisher
  4. Zavodszky E, Seaman M, Moreau K, Jimenez Sanchez M, Breusegem S, Harbour M, et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun. 2014;5:3828 pubmed publisher