This is a Validated Antibody Database (VAD) review about human WAS, based on 11 published articles (read how Labome selects the articles), using WAS antibody in all methods. It is aimed to help Labome visitors find the most suited WAS antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
WAS synonym: IMD2; SCNX; THC; THC1; WASP; WASPA

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (F-8)
  • western blot knockout validation; human; loading ...; fig 1a
  • western blot knockout validation; mouse; loading ...; fig 1b
Santa Cruz Biotechnology WAS antibody (SantaCruz, SC-365859) was used in western blot knockout validation on human samples (fig 1a) and in western blot knockout validation on mouse samples (fig 1b). J Clin Invest (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-9)
  • western blot knockout validation; mouse; 1:200; fig 4
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-13139) was used in western blot knockout validation on mouse samples at 1:200 (fig 4). Nat Commun (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-9)
  • western blot; mouse; loading ...; fig 4l
Santa Cruz Biotechnology WAS antibody (Santa Cruz Biotechnology, sc-13139) was used in western blot on mouse samples (fig 4l). Clin Transl Med (2022) ncbi
mouse monoclonal (F-8)
  • western blot; human; fig 6g
Santa Cruz Biotechnology WAS antibody (Santa Cruz Biotechnology Inc, F-8) was used in western blot on human samples (fig 6g). J Clin Invest (2018) ncbi
mouse monoclonal (D-1)
  • chromatin immunoprecipitation; human; loading ...; fig 2g
  • immunocytochemistry; human; 1:50; loading ...; fig 2c
  • western blot; human; loading ...; fig s4g
  • immunocytochemistry; mouse; 1:50; fig s1f
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-5300) was used in chromatin immunoprecipitation on human samples (fig 2g), in immunocytochemistry on human samples at 1:50 (fig 2c), in western blot on human samples (fig s4g) and in immunocytochemistry on mouse samples at 1:50 (fig s1f). Nature (2018) ncbi
mouse monoclonal (F-8)
  • western blot; human; loading ...; fig s2b
Santa Cruz Biotechnology WAS antibody (SantaCruz, sc-365859) was used in western blot on human samples (fig s2b). J Exp Med (2016) ncbi
mouse monoclonal (F-8)
  • western blot knockout validation; human; loading ...; fig 1a
  • western blot knockout validation; mouse; loading ...; fig 1b
Santa Cruz Biotechnology WAS antibody (SantaCruz, SC-365859) was used in western blot knockout validation on human samples (fig 1a) and in western blot knockout validation on mouse samples (fig 1b). J Clin Invest (2016) ncbi
mouse monoclonal (B-9)
  • western blot knockout validation; mouse; 1:200; fig 4
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-13139) was used in western blot knockout validation on mouse samples at 1:200 (fig 4). Nat Commun (2016) ncbi
mouse monoclonal (F-8)
  • immunocytochemistry; human; loading ...; fig 5b
  • western blot; human; loading ...; fig 5a
Santa Cruz Biotechnology WAS antibody (Santa Cruz, SC365859) was used in immunocytochemistry on human samples (fig 5b) and in western blot on human samples (fig 5a). Sci Rep (2015) ncbi
mouse monoclonal (B-9)
  • western blot; mouse; fig 6
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-13139) was used in western blot on mouse samples (fig 6). PLoS ONE (2015) ncbi
mouse monoclonal (B-9)
  • western blot; human; fig 5
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-13139) was used in western blot on human samples (fig 5). Mol Ther Methods Clin Dev (2015) ncbi
mouse monoclonal (B-9)
  • immunocytochemistry; human; fig 1
Santa Cruz Biotechnology WAS antibody (Santa Cruz, sc-13139) was used in immunocytochemistry on human samples (fig 1). Cancer Lett (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 10 ug/ml; fig s5
In order to determine the role of TRPM7 channel function defects and how they deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture, Cell Signaling Technology WAS antibody (CellSignaling, 4860) was used in immunocytochemistry on mouse samples at 10 ug/ml (fig s5). Nat Commun (2016) ncbi
Articles Reviewed
  1. Zhu Y, Gu H, Yang L, Li N, Chen Q, Kang D, et al. Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clin Transl Med. 2022;12:e887 pubmed publisher
  2. Keszei M, Record J, Kritikou J, Wurzer H, Geyer C, Thiemann M, et al. Constitutive activation of WASp in X-linked neutropenia renders neutrophils hyperactive. J Clin Invest. 2018;128:4115-4131 pubmed publisher
  3. Schrank B, Aparicio T, Li Y, Chang W, Chait B, Gundersen G, et al. Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature. 2018;559:61-66 pubmed publisher
  4. Baron L, Paatero A, Morel J, Impens F, Guenin Macé L, Saint Auret S, et al. Mycolactone subverts immunity by selectively blocking the Sec61 translocon. J Exp Med. 2016;213:2885-2896 pubmed
  5. Janssen E, Tohme M, Hedayat M, Leick M, Kumari S, Ramesh N, et al. A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton. J Clin Invest. 2016;126:3837-3851 pubmed publisher
  6. Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
  7. Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
  8. Shrivastava A, Prasad A, Kuzontkoski P, Yu J, Groopman J. Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements. Sci Rep. 2015;5:16833 pubmed publisher
  9. Silva O, Crocetti J, Humphries L, Burkhardt J, Miceli M. Discs Large Homolog 1 Splice Variants Regulate p38-Dependent and -Independent Effector Functions in CD8+ T Cells. PLoS ONE. 2015;10:e0133353 pubmed publisher
  10. Wielgosz M, Kim Y, Carney G, Zhan J, Reddivari M, Coop T, et al. Generation of a lentiviral vector producer cell clone for human Wiskott-Aldrich syndrome gene therapy. Mol Ther Methods Clin Dev. 2015;2:14063 pubmed publisher
  11. Ward J, Ha J, Jayaraman M, Dhanasekaran D. LPA-mediated migration of ovarian cancer cells involves translocalization of Gαi2 to invadopodia and association with Src and β-pix. Cancer Lett. 2015;356:382-91 pubmed publisher