This is a Validated Antibody Database (VAD) review about human VPS35, based on 26 published articles (read how Labome selects the articles), using VPS35 antibody in all methods. It is aimed to help Labome visitors find the most suited VPS35 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
VPS35 synonym: MEM3; PARK17

Knockout validation
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 4a
Abcam VPS35 antibody (Abcam, ab57632) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4a). Cell Rep (2022) ncbi
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2b
  • immunocytochemistry; human; 1:100; loading ...; fig s4a
Abcam VPS35 antibody (Abcam, ab97545) was used in western blot knockout validation on human samples at 1:1000 (fig 2b) and in immunocytochemistry on human samples at 1:100 (fig s4a). EMBO J (2018) ncbi
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 4a
Abcam VPS35 antibody (Abcam, ab57632) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4a). Cell Rep (2022) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; loading ...; fig s2a
Abcam VPS35 antibody (Abcam, ab57632) was used in immunocytochemistry on mouse samples (fig s2a). Cell Rep (2021) ncbi
domestic rabbit monoclonal (EPR11501(B))
  • western blot; mouse; loading ...; fig 5c
Abcam VPS35 antibody (Abcam, a157220) was used in western blot on mouse samples (fig 5c). Aging Cell (2021) ncbi
domestic goat polyclonal
  • western blot; mouse; loading ...; fig 1d
Abcam VPS35 antibody (Abcam, ab10099) was used in western blot on mouse samples (fig 1d). Cell Death Dis (2020) ncbi
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 2a
Abcam VPS35 antibody (AbCam, ab10099) was used in immunohistochemistry - paraffin section on mouse samples (fig 2c) and in western blot on mouse samples (fig 2a). Mol Neurodegener (2020) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:500; loading ...; fig 1d
Abcam VPS35 antibody (Abcam, ab97545) was used in western blot on rat samples at 1:500 (fig 1d). Cell Rep (2019) ncbi
domestic goat polyclonal
  • immunocytochemistry; human; 1:250; loading ...; fig 1d
Abcam VPS35 antibody (Abcam, ab10099) was used in immunocytochemistry on human samples at 1:250 (fig 1d). Nat Commun (2019) ncbi
domestic goat polyclonal
  • immunocytochemistry; human; 1:250; loading ...; fig s2
Abcam VPS35 antibody (Abcam, ab10099) was used in immunocytochemistry on human samples at 1:250 (fig s2). J Cell Sci (2019) ncbi
domestic goat polyclonal
  • immunocytochemistry; mouse; 1:150; fig 3b
Abcam VPS35 antibody (Abcam, ab10099) was used in immunocytochemistry on mouse samples at 1:150 (fig 3b). J Neurosci (2018) ncbi
domestic rabbit monoclonal (EPR11501(B))
  • western blot; mouse; 1:1000; loading ...; fig 4a
Abcam VPS35 antibody (Abcam, ab157220) was used in western blot on mouse samples at 1:1000 (fig 4a). Biochem J (2018) ncbi
domestic goat polyclonal
  • western blot; human; 1:1000; loading ...; fig 2b
Abcam VPS35 antibody (Abcam, ab10099) was used in western blot on human samples at 1:1000 (fig 2b). J Exp Med (2018) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 2b
  • immunocytochemistry; human; 1:100; loading ...; fig s4a
Abcam VPS35 antibody (Abcam, ab97545) was used in western blot knockout validation on human samples at 1:1000 (fig 2b) and in immunocytochemistry on human samples at 1:100 (fig s4a). EMBO J (2018) ncbi
domestic goat polyclonal
  • western blot; human; 1:1000; fig 5c
Abcam VPS35 antibody (Abcam, ab10099) was used in western blot on human samples at 1:1000 (fig 5c). Nat Commun (2017) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig s6a
Abcam VPS35 antibody (Abcam, ab57632) was used in western blot on human samples (fig s6a). Mol Biol Cell (2018) ncbi
domestic goat polyclonal
  • immunocytochemistry; human; loading ...; fig s17
In order to show that 2-hydroxyoleic acid treatment alters cell signaling and intracellular transport, Abcam VPS35 antibody (AbCam, ab10099) was used in immunocytochemistry on human samples (fig s17). Oncotarget (2016) ncbi
domestic goat polyclonal
  • immunocytochemistry; mouse; 1:150; fig 3
  • western blot; mouse; fig 3
In order to study a phosphoinositide-based mechanism that Arf6 uses to control retromer traffic and intracellular cholesterol distribution, Abcam VPS35 antibody (Abcam, ab10099) was used in immunocytochemistry on mouse samples at 1:150 (fig 3) and in western blot on mouse samples (fig 3). Nat Commun (2016) ncbi
domestic goat polyclonal
  • immunocytochemistry; mouse; 1:1000; fig 4
In order to analyze how WASH- and CCC-mediated endosomal sorting of LDLr is needed for regular clearance of circulating LDL, Abcam VPS35 antibody (Abcam, ab10099-100) was used in immunocytochemistry on mouse samples at 1:1000 (fig 4). Nat Commun (2016) ncbi
mouse monoclonal
  • proximity ligation assay; human; 1:500; fig 5
  • western blot; human
Abcam VPS35 antibody (Abcam, 57632) was used in proximity ligation assay on human samples at 1:500 (fig 5) and in western blot on human samples . PLoS Pathog (2015) ncbi
mouse monoclonal
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
In order to demonstrate that the Parkinson's disease-causing D620N mutation in VPS35 restricts WASH complex recruitment to endosomes and alters autophagosome formation, Abcam VPS35 antibody (Abcam, ab57632) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000. Nat Commun (2014) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000
Abcam VPS35 antibody (Abcam, ab57632) was used in western blot on mouse samples at 1:1000. Neurobiol Dis (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-5)
  • immunocytochemistry; human; loading ...; fig 7d
  • western blot; human; 1:1000; loading ...; fig 7c
Santa Cruz Biotechnology VPS35 antibody (Santa Cruz Biotechnology, sc-374372) was used in immunocytochemistry on human samples (fig 7d) and in western blot on human samples at 1:1000 (fig 7c). Nat Commun (2019) ncbi
mouse monoclonal (B-5)
  • immunocytochemistry; human; loading ...; fig 2c
Santa Cruz Biotechnology VPS35 antibody (Santa Cruz, SC-374372) was used in immunocytochemistry on human samples (fig 2c). Cell Mol Life Sci (2018) ncbi
mouse monoclonal (B-5)
  • western blot; human; fig 1c
Santa Cruz Biotechnology VPS35 antibody (SantaCruz, sc-374372) was used in western blot on human samples (fig 1c). Hum Mol Genet (2017) ncbi
mouse monoclonal (B-5)
  • western blot; human; 1:500
In order to demonstrate that the Parkinson's disease-causing D620N mutation in VPS35 restricts WASH complex recruitment to endosomes and alters autophagosome formation, Santa Cruz Biotechnology VPS35 antibody (Santa Cruz, sc-374372) was used in western blot on human samples at 1:500. Nat Commun (2014) ncbi
Abnova
mouse monoclonal (2D3)
  • western blot; human; fig e8a
In order to show the role of Pex3 and Pex14 in generating human peroxisomes, Abnova VPS35 antibody (Novus, 2D3) was used in western blot on human samples (fig e8a). Nature (2017) ncbi
domestic goat polyclonal
  • western blot; mouse; loading ...; fig 10s1
In order to find that CNS-specific snx6-knockout mice exhibit deficits in spatial learning and memory, accompanied with loss of spines from distal dendrites of hippocampal CA1 pyramidal cells, Abnova VPS35 antibody (Abnova, PAB7499) was used in western blot on mouse samples (fig 10s1). elife (2017) ncbi
mouse monoclonal (2D3)
  • immunocytochemistry; rat; 1:500; fig 3
In order to study how cellular localization affects DHHC5 activity, Abnova VPS35 antibody (Abnova, H000055737-M02) was used in immunocytochemistry on rat samples at 1:500 (fig 3). Nat Commun (2015) ncbi
Articles Reviewed
  1. Qureshi Y, Berman D, Marsh S, Klein R, Patel V, Simoes S, et al. The neuronal retromer can regulate both neuronal and microglial phenotypes of Alzheimer's disease. Cell Rep. 2022;38:110262 pubmed publisher
  2. Simoes S, Guo J, Buitrago L, Qureshi Y, Feng X, Kothiya M, et al. Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling. Cell Rep. 2021;37:110182 pubmed publisher
  3. Niu M, Zhao F, Bondelid K, Siedlak S, Torres S, Fujioka H, et al. VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease. Aging Cell. 2021;20:e13347 pubmed publisher
  4. Chiu C, Weng Y, Huang Y, Chen R, Liu Y, Yeh T, et al. (D620N) VPS35 causes the impairment of Wnt/β-catenin signaling cascade and mitochondrial dysfunction in a PARK17 knockin mouse model. Cell Death Dis. 2020;11:1018 pubmed publisher
  5. Li J, Chiu J, Ramanjulu M, Blass B, Pratico D. A pharmacological chaperone improves memory by reducing Aβ and tau neuropathology in a mouse model with plaques and tangles. Mol Neurodegener. 2020;15:1 pubmed publisher
  6. Halff E, Szulc B, Lesept F, Kittler J. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Rep. 2019;29:2599-2607.e6 pubmed publisher
  7. Pietila M, Sahgal P, Peuhu E, Jäntti N, Paatero I, Närvä E, et al. SORLA regulates endosomal trafficking and oncogenic fitness of HER2. Nat Commun. 2019;10:2340 pubmed publisher
  8. Sahgal P, Alanko J, Icha J, Paatero I, Hamidi H, Arjonen A, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. J Cell Sci. 2019;132: pubmed publisher
  9. Sapmaz A, Berlin I, Bos E, Wijdeven R, Janssen H, Konietzny R, et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun. 2019;10:1454 pubmed publisher
  10. Appel J, Ye S, Tang F, Sun D, Zhang H, Mei L, et al. Increased Microglial Activity, Impaired Adult Hippocampal Neurogenesis, and Depressive-like Behavior in Microglial VPS35-Depleted Mice. J Neurosci. 2018;38:5949-5968 pubmed publisher
  11. Mir R, Tonelli F, Lis P, MacArtney T, Polinski N, Martinez T, et al. The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human. Biochem J. 2018;475:1861-1883 pubmed publisher
  12. Carpier J, Zucchetti A, Bataille L, Dogniaux S, Shafaq Zadah M, Bardin S, et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med. 2018;215:1245-1265 pubmed publisher
  13. Mukadam A, Breusegem S, Seaman M. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing. Cell Mol Life Sci. 2018;75:2613-2625 pubmed publisher
  14. Jimenez Orgaz A, Kvainickas A, Nägele H, Denner J, Eimer S, Dengjel J, et al. Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy. EMBO J. 2018;37:235-254 pubmed publisher
  15. Bärlocher K, Hutter C, Swart A, Steiner B, Welin A, Hohl M, et al. Structural insights into Legionella RidL-Vps29 retromer subunit interaction reveal displacement of the regulator TBC1D5. Nat Commun. 2017;8:1543 pubmed publisher
  16. Toh W, Chia P, Hossain M, Gleeson P. GGA1 regulates signal-dependent sorting of BACE1 to recycling endosomes, which moderates Aβ production. Mol Biol Cell. 2018;29:191-208 pubmed publisher
  17. Sugiura A, Mattie S, Prudent J, McBride H. Newly born peroxisomes are a hybrid of mitochondrial and ER-derived pre-peroxisomes. Nature. 2017;542:251-254 pubmed publisher
  18. Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher
  19. Kim M, Deng H, Wong Y, Siddique T, Krainc D. The Parkinson's disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking. Hum Mol Genet. 2017;26:729-741 pubmed publisher
  20. Torgersen M, Klokk T, Kavaliauskiene S, Klose C, Simons K, Skotland T, et al. The anti-tumor drug 2-hydroxyoleic acid (Minerval) stimulates signaling and retrograde transport. Oncotarget. 2016;7:86871-86888 pubmed publisher
  21. Marquer C, Tian H, Yi J, Bastien J, Dall Armi C, Yang Klingler Y, et al. Arf6 controls retromer traffic and intracellular cholesterol distribution via a phosphoinositide-based mechanism. Nat Commun. 2016;7:11919 pubmed publisher
  22. Bartuzi P, Billadeau D, Favier R, Rong S, Dekker D, Fedoseienko A, et al. CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL. Nat Commun. 2016;7:10961 pubmed publisher
  23. Brigidi G, Santyr B, Shimell J, Jovellar B, Bamji S. Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5. Nat Commun. 2015;6:8200 pubmed publisher
  24. Popa A, Zhang W, Harrison M, Goodner K, Kazakov T, Goodwin E, et al. Direct binding of retromer to human papillomavirus type 16 minor capsid protein L2 mediates endosome exit during viral infection. PLoS Pathog. 2015;11:e1004699 pubmed publisher
  25. Zavodszky E, Seaman M, Moreau K, Jimenez Sanchez M, Breusegem S, Harbour M, et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun. 2014;5:3828 pubmed publisher
  26. Morabito M, Berman D, Schneider R, Zhang Y, Leibel R, Small S. Hyperleucinemia causes hippocampal retromer deficiency linking diabetes to Alzheimer's disease. Neurobiol Dis. 2014;65:188-92 pubmed publisher