This is a Validated Antibody Database (VAD) review about human VHL, based on 15 published articles (read how Labome selects the articles), using VHL antibody in all methods. It is aimed to help Labome visitors find the most suited VHL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
VHL synonym: HRCA1; RCA1; VHL1; pVHL; von Hippel-Lindau disease tumor suppressor; elongin binding protein; protein G7; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase

Knockout validation
MyBioSource
rabbit polyclonal
  • western blot knockout validation; human; fig 1
In order to determine the regulation of von Hippel Lindau proteostasis and function by phosphorylation-dependent cleavage, MyBioSource VHL antibody (MyBioSource, MBS125962) was used in western blot knockout validation on human samples (fig 1). Oncogene (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (VHL40)
  • western blot; human; loading ...; fig 1d
Santa Cruz Biotechnology VHL antibody (Santa Cruz, sc-135657) was used in western blot on human samples (fig 1d). Am J Transl Res (2017) ncbi
mouse monoclonal (VHL40)
  • western blot; human; loading ...; fig 3b
In order to research the effect of VHL on immune response against renal cell carcinoma and its mechanism, Santa Cruz Biotechnology VHL antibody (Santa Cruz, sc-135657) was used in western blot on human samples (fig 3b). J Cell Biol (2017) ncbi
mouse monoclonal (D-7)
  • western blot; human; fig 4
Santa Cruz Biotechnology VHL antibody (Santa Cruz, D-7) was used in western blot on human samples (fig 4). J Cell Sci (2016) ncbi
mouse monoclonal (G-7)
  • western blot; human
Santa Cruz Biotechnology VHL antibody (Santa Cruz Biotechnology, SC-17780) was used in western blot on human samples . Br J Pharmacol (2015) ncbi
mouse monoclonal (D-7)
  • immunocytochemistry; human; fig 1
Santa Cruz Biotechnology VHL antibody (Santa Cruz, sc-55506) was used in immunocytochemistry on human samples (fig 1). Oncotarget (2015) ncbi
MyBioSource
rabbit polyclonal
  • western blot knockout validation; human; fig 1
In order to determine the regulation of von Hippel Lindau proteostasis and function by phosphorylation-dependent cleavage, MyBioSource VHL antibody (MyBioSource, MBS125962) was used in western blot knockout validation on human samples (fig 1). Oncogene (2016) ncbi
Invitrogen
mouse monoclonal (Ig33)
  • immunohistochemistry - paraffin section; human; 1:200
In order to study the effect of genetic alterations and protein expression of Von Hipple-Lindau and the C1772T SNP of HIF-1alpha gene on prognosis in early-stage clear cell renal cell carcinoma, Invitrogen VHL antibody (Lab Vision, Ig33) was used in immunohistochemistry - paraffin section on human samples at 1:200. Med Oncol (2014) ncbi
BD Biosciences
mouse monoclonal (Ig32)
  • other; human; loading ...; fig 4c
BD Biosciences VHL antibody (BD Biosciences, 556347) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
mouse monoclonal (Ig32)
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., BD Biosciences VHL antibody (BD Pharmingen, 556347) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
mouse monoclonal (Ig32)
  • reverse phase protein lysate microarray; human; loading ...; fig 3a
In order to describe the features of 228 primary cervical cancers, BD Biosciences VHL antibody (BD Pharmingen, 556347) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi
mouse monoclonal (S2-647)
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 1
BD Biosciences VHL antibody (BD Pharmingen, 564183) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples (fig 1). Int J Cancer (2016) ncbi
mouse monoclonal (Ig32)
  • western blot; human; fig 8
BD Biosciences VHL antibody (BD Biosciences, 556347) was used in western blot on human samples (fig 8). J Biol Chem (2016) ncbi
mouse monoclonal (Ig32)
  • immunoprecipitation; human; fig 4
In order to report the contribution of DYRK1A and DYRK1B in cancer stem cells, BD Biosciences VHL antibody (BD, 556347) was used in immunoprecipitation on human samples (fig 4). Nature (2016) ncbi
mouse monoclonal (Ig32)
  • immunohistochemistry; mouse; 1:100; fig 9a
In order to determine the role of Vhl in mesenchymal cells, BD Biosciences VHL antibody (BD Biosciences, BD556347) was used in immunohistochemistry on mouse samples at 1:100 (fig 9a). Am J Pathol (2015) ncbi
mouse monoclonal (Ig32)
  • western blot; mouse; 1:500; fig 5,6
BD Biosciences VHL antibody (BD Bioscience, Ig32) was used in western blot on mouse samples at 1:500 (fig 5,6). Nat Commun (2015) ncbi
mouse monoclonal (Ig32)
  • immunohistochemistry - paraffin section; human; 1:50
In order to study the effect of genetic alterations and protein expression of Von Hipple-Lindau and the C1772T SNP of HIF-1alpha gene on prognosis in early-stage clear cell renal cell carcinoma, BD Biosciences VHL antibody (BD-Bioscience, Ig32) was used in immunohistochemistry - paraffin section on human samples at 1:50. Med Oncol (2014) ncbi
Articles Reviewed
  1. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  2. Li S, Liu H, Tang S, Li X, Wang X. MicroRNA-150 regulates glycolysis by targeting von Hippel-Lindau in glioma cells. Am J Transl Res. 2017;9:1058-1066 pubmed
  3. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  4. Labrousse Arias D, Martínez Alonso E, Corral Escariz M, Bienes Martínez R, Berridy J, Serrano Oviedo L, et al. VHL promotes immune response against renal cell carcinoma via NF-κB-dependent regulation of VCAM-1. J Cell Biol. 2017;216:835-847 pubmed publisher
  5. . Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
  6. German P, Bai S, Liu X, Sun M, Zhou L, Kalra S, et al. Phosphorylation-dependent cleavage regulates von Hippel Lindau proteostasis and function. Oncogene. 2016;35:4973-80 pubmed publisher
  7. Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer. 2016;139:396-403 pubmed publisher
  8. Heir P, Srikumar T, Bikopoulos G, Bunda S, Poon B, Lee J, et al. Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling. J Biol Chem. 2016;291:7357-72 pubmed publisher
  9. Chen K, Zeng J, Xiao H, Huang C, Hu J, Yao W, et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1α. J Cell Sci. 2016;129:817-30 pubmed publisher
  10. Lee S, Frattini V, Bansal M, Castano A, Sherman D, Hutchinson K, et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016;529:172-7 pubmed publisher
  11. Mangiavini L, Merceron C, Araldi E, Khatri R, Gerard O Riley R, Wilson T, et al. Fibrosis and hypoxia-inducible factor-1α-dependent tumors of the soft tissue on loss of von Hippel-Lindau in mesenchymal progenitors. Am J Pathol. 2015;185:3090-101 pubmed publisher
  12. Kim B, Lee J, Choi J, Park D, Song H, Park T, et al. Imidazole-based alkaloid derivative LCB54-0009 suppresses ocular angiogenesis and lymphangiogenesis in models of experimental retinopathy and corneal neovascularization. Br J Pharmacol. 2015;172:3875-89 pubmed publisher
  13. Chen K, Yu G, Gumireddy K, Li A, Yao W, Gao L, et al. ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer. Oncotarget. 2015;6:6959-76 pubmed
  14. Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
  15. Lessi F, Mazzanti C, Tomei S, Di Cristofano C, Minervini A, Menicagli M, et al. VHL and HIF-1?: gene variations and prognosis in early-stage clear cell renal cell carcinoma. Med Oncol. 2014;31:840 pubmed publisher