This is a Validated Antibody Database (VAD) review about human VAPB, based on 7 published articles (read how Labome selects the articles), using VAPB antibody in all methods. It is aimed to help Labome visitors find the most suited VAPB antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
VAPB synonym: ALS8; VAMP-B; VAP-B

Proteintech Group
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig 1a
  • western blot; human; loading ...; fig 3c
Proteintech Group VAPB antibody (Proteintech Group, 14477-1-AP) was used in immunocytochemistry on human samples at 1:1000 (fig 1a) and in western blot on human samples (fig 3c). J Biol Chem (2019) ncbi
mouse monoclonal (4F6A6)
  • proximity ligation assay; human; 1:200; loading ...; fig 2d
  • western blot; human; loading ...; fig 2f
  • proximity ligation assay; African green monkey; 1:200; loading ...; fig 1b
Proteintech Group VAPB antibody (Proteintech, 66191-1-Ig) was used in proximity ligation assay on human samples at 1:200 (fig 2d), in western blot on human samples (fig 2f) and in proximity ligation assay on African green monkey samples at 1:200 (fig 1b). Contact (Thousand Oaks) (2019) ncbi
mouse monoclonal (4F6A6)
  • immunocytochemistry; human; 1:200; loading ...; fig 3b
  • western blot; human; loading ...; fig 2d
Proteintech Group VAPB antibody (Proteintech, 66191-1) was used in immunocytochemistry on human samples at 1:200 (fig 3b) and in western blot on human samples (fig 2d). Cells (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 5e
  • western blot; human; loading ...; fig 5c
In order to demonstrate that RIDalpha utilizes ORP1L to fine-tune lipid raft cholesterol during adenovirus infection, Proteintech Group VAPB antibody (Proteintech, 14477-1-AP) was used in immunocytochemistry on human samples (fig 5e) and in western blot on human samples (fig 5c). J Virol (2017) ncbi
R&D Systems
mouse monoclonal (736904)
  • flow cytometry; mouse
R&D Systems VAPB antibody (R&D Systems, MAB58551) was used in flow cytometry on mouse samples . Nat Commun (2019) ncbi
mouse monoclonal (736904)
  • proximity ligation assay; mouse
In order to study the role of PTPIP51 in memory processing, R&D Systems VAPB antibody (r & D, mab58551) was used in proximity ligation assay on mouse samples . Mol Cell Neurosci (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4s3e
MilliporeSigma VAPB antibody (Sigma, HPA013144) was used in western blot on human samples at 1:1000 (fig 4s3e). elife (2019) ncbi
Articles Reviewed
  1. Höglinger D, Burgoyne T, Sanchez Heras E, Hartwig P, Colaco A, Newton J, et al. NPC1 regulates ER contacts with endocytic organelles to mediate cholesterol egress. Nat Commun. 2019;10:4276 pubmed publisher
  2. James C, Müller M, Goldberg M, Lenz C, Urlaub H, Kehlenbach R. Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane. J Biol Chem. 2019;294:16241-16254 pubmed publisher
  3. Bishop A, Kamoshita M, Passmore J, Hacker C, Schrader T, Waterham H, et al. Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells. Contact (Thousand Oaks). 2019;2: pubmed publisher
  4. Yeshaw W, van der Zwaag M, Pinto F, Lahaye L, Faber A, Gómez Sánchez R, et al. Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility. elife. 2019;8: pubmed publisher
  5. Saiz Ros N, Czapiewski R, Epifano I, Stevenson A, Swanson S, Dixon C, et al. Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication. Cells. 2019;8: pubmed publisher
  6. Cianciola N, Chung S, Manor D, Carlin C. Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum. J Virol. 2017;91: pubmed publisher
  7. Brobeil A, Viard M, Petri M, Steger K, Tag C, Wimmer M. Memory and PTPIP51--a new protein in hippocampus and cerebellum. Mol Cell Neurosci. 2015;64:61-73 pubmed publisher