This is a Validated Antibody Database (VAD) review about human VAMP8, based on 10 published articles (read how Labome selects the articles), using VAMP8 antibody in all methods. It is aimed to help Labome visitors find the most suited VAMP8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
VAMP8 synonym: EDB; VAMP-8; vesicle-associated membrane protein 8; vesicle-associated membrane protein 8 (endobrevin)

Knockout validation
Synaptic Systems
rabbit polyclonal (/)
  • western blot knockout validation; mouse; fig 5a
In order to elucidate VAMP8- and VAMP2-mediated zymogen granules exocytosis, Synaptic Systems VAMP8 antibody (Synaptic Systems, 104303) was used in western blot knockout validation on mouse samples (fig 5a). J Biol Chem (2014) ncbi
Abcam
rabbit monoclonal (EP2629Y)
  • western blot; human; 1:1000; loading ...; fig 4b
Abcam VAMP8 antibody (Abcam, ab76021) was used in western blot on human samples at 1:1000 (fig 4b). Nat Commun (2017) ncbi
rabbit monoclonal (EP2629Y)
  • immunoprecipitation; human; loading ...; fig 6d
  • immunocytochemistry; human; loading ...; fig 6g
  • western blot; human; 1:1000; loading ...; fig 6d
In order to research the effect of methyl-beta-cyclodextrin on autophagy flux in Niemann-Pick C1-deficient cells, Abcam VAMP8 antibody (Abcam, ab76021) was used in immunoprecipitation on human samples (fig 6d), in immunocytochemistry on human samples (fig 6g) and in western blot on human samples at 1:1000 (fig 6d). Autophagy (2017) ncbi
rabbit monoclonal (EP2629Y)
  • western blot; human; 1:800; loading ...; fig 3a
Abcam VAMP8 antibody (Abcam, ab76021) was used in western blot on human samples at 1:800 (fig 3a). Exp Ther Med (2016) ncbi
rabbit monoclonal (EP2629Y)
  • western blot; human; fig 5e
In order to show that syntaxin-17 is required for the delivery of stress-induced PINK1/parkin-dependent mitochondrial-derived vesicles to the late endosome/lysosome, Abcam VAMP8 antibody (Abcam, ab76021) was used in western blot on human samples (fig 5e). J Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; rat
Abcam VAMP8 antibody (Abcam, ab6186) was used in western blot on rat samples . Biochem J (2013) ncbi
Synaptic Systems
rabbit polyclonal (/)
  • immunocytochemistry; rat; fig 1
  • western blot; rat; fig s5
Synaptic Systems VAMP8 antibody (Synaptic Systems, 104303) was used in immunocytochemistry on rat samples (fig 1) and in western blot on rat samples (fig s5). EMBO J (2016) ncbi
rabbit polyclonal (/)
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 2
In order to investigate a mechanism of late endosomal maturation, Synaptic Systems VAMP8 antibody (Synaptic Systems, 104302) was used in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 2). Mol Biol Cell (2016) ncbi
rabbit polyclonal (/)
  • western blot knockout validation; mouse; fig 5a
In order to elucidate VAMP8- and VAMP2-mediated zymogen granules exocytosis, Synaptic Systems VAMP8 antibody (Synaptic Systems, 104303) was used in western blot knockout validation on mouse samples (fig 5a). J Biol Chem (2014) ncbi
R&D Systems
goat polyclonal
  • western blot; human; 1 ug/ml; loading ...; fig 6g
R&D Systems VAMP8 antibody (R&D Systems, AF5354) was used in western blot on human samples at 1 ug/ml (fig 6g). Mol Biol Cell (2017) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Bethyl VAMP8 antibody (Bethyl Laboratories, A304-350A-T) was used in western blot on human samples at 1:1000 (fig 3b). Nat Commun (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-12)
  • western blot; human; 1:500; loading ...; fig 5-s1c
Santa Cruz Biotechnology VAMP8 antibody (SantaCruz, sc-166820) was used in western blot on human samples at 1:500 (fig 5-s1c). elife (2017) ncbi
Articles Reviewed
  1. Bhardwaj A, Yang Y, Ueberheide B, Smith S. Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation. Nat Commun. 2017;8:2214 pubmed publisher
  2. Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
  3. Verboogen D, González Mancha N, ter Beest M, van den Bogaart G. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. elife. 2017;6: pubmed publisher
  4. Woo S, James D, Martin T. Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles. Mol Biol Cell. 2017;28:792-808 pubmed publisher
  5. Xie X, Liu C, Lin W, Zhan B, Dong C, Song Z, et al. Deep vein thrombosis is accurately predicted by comprehensive analysis of the levels of microRNA-96 and plasma D-dimer. Exp Ther Med. 2016;12:1896-1900 pubmed
  6. McLelland G, Lee S, McBride H, Fon E. Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. J Cell Biol. 2016;214:275-91 pubmed publisher
  7. Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner R, Wang A, et al. Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion. EMBO J. 2016;35:1810-21 pubmed publisher
  8. He J, Johnson J, Monfregola J, Ramadass M, Pestonjamasp K, Napolitano G, et al. Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses. Mol Biol Cell. 2016;27:572-87 pubmed publisher
  9. Messenger S, Falkowski M, Thomas D, Jones E, Hong W, Gaisano H, et al. Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway. J Biol Chem. 2014;289:28040-53 pubmed publisher
  10. Yang Y, Oh J, Heo P, Shin J, Kong B, Shin J, et al. Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes. Biochem J. 2013;450:537-46 pubmed publisher