This is a Validated Antibody Database (VAD) review about human USP9X, based on 9 published articles (read how Labome selects the articles), using USP9X antibody in all methods. It is aimed to help Labome visitors find the most suited USP9X antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
USP9X synonym: DFFRX; FAF; FAM; MRX99; MRXS99F

Knockout validation
Abcam
domestic rabbit monoclonal (EPR13809(B))
  • western blot knockout validation; human; 1:1500; loading ...; fig s5f
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1c
Abcam USP9X antibody (Abcam, ab180191) was used in western blot knockout validation on human samples at 1:1500 (fig s5f) and in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1c). J Biomed Sci (2021) ncbi
Genentech
mouse monoclonal (4B3.1.1)
  • immunocytochemistry knockout validation; mouse; 0.2 ug/ml; loading ...; fig 1d
  • western blot knockout validation; mouse; loading ...; fig 3a
  • immunoprecipitation; mouse; loading ...; fig 3e
Genentech USP9X antibody (Genentech, 4B3.1.1) was used in immunocytochemistry knockout validation on mouse samples at 0.2 ug/ml (fig 1d), in western blot knockout validation on mouse samples (fig 3a) and in immunoprecipitation on mouse samples (fig 3e). J Immunol (2016) ncbi
Genentech
mouse monoclonal (4B3.1.1)
  • western blot knockout validation; mouse; fig 1
In order to analyze deubiquitinization Usp9X and its regulation of proximal T cell receptor signaling and tolerance induction, Genentech USP9X antibody (Genentech, 4B3.1.1) was used in western blot knockout validation on mouse samples (fig 1). J Exp Med (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR13809(B))
  • western blot knockout validation; human; 1:1500; loading ...; fig s5f
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1c
Abcam USP9X antibody (Abcam, ab180191) was used in western blot knockout validation on human samples at 1:1500 (fig s5f) and in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1c). J Biomed Sci (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
Abcam USP9X antibody (Abcam, ab19879) was used in western blot on human samples (fig 1c). J Clin Invest (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 3
Abcam USP9X antibody (Abcam, ab19879) was used in immunohistochemistry on mouse samples at 1:100 (fig 3). PLoS ONE (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-12)
  • western blot; human; loading ...; fig 3b
Santa Cruz Biotechnology USP9X antibody (Santa, sc-365353) was used in western blot on human samples (fig 3b). Cancer Cell Int (2019) ncbi
Abnova
mouse monoclonal (1C4)
  • western blot; human; fig 1
In order to study the activation mechanisms of core Hippo pathway components, Abnova USP9X antibody (Abnova, H00008239-M01) was used in western blot on human samples (fig 1). EMBO Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4Y7W)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology USP9X antibody (Cell Signaling, 14898) was used in western blot on human samples (fig 1d). Theranostics (2018) ncbi
domestic rabbit monoclonal (D4Y7W)
  • western blot; human; 1:1000; fig 1d
Cell Signaling Technology USP9X antibody (Cell Signaling, 14898) was used in western blot on human samples at 1:1000 (fig 1d). Nat Commun (2018) ncbi
Genentech
mouse monoclonal (4B3.1.1)
  • immunocytochemistry knockout validation; mouse; 0.2 ug/ml; loading ...; fig 1d
  • western blot knockout validation; mouse; loading ...; fig 3a
  • immunoprecipitation; mouse; loading ...; fig 3e
Genentech USP9X antibody (Genentech, 4B3.1.1) was used in immunocytochemistry knockout validation on mouse samples at 0.2 ug/ml (fig 1d), in western blot knockout validation on mouse samples (fig 3a) and in immunoprecipitation on mouse samples (fig 3e). J Immunol (2016) ncbi
mouse monoclonal (4B3.1.1)
  • western blot knockout validation; mouse; fig 1
In order to analyze deubiquitinization Usp9X and its regulation of proximal T cell receptor signaling and tolerance induction, Genentech USP9X antibody (Genentech, 4B3.1.1) was used in western blot knockout validation on mouse samples (fig 1). J Exp Med (2014) ncbi
Articles Reviewed
  1. Chen W, Song J, Liu S, Tang B, Shen L, Zhu J, et al. USP9X promotes apoptosis in cholangiocarcinoma by modulation expression of KIF1Bβ via deubiquitinating EGLN3. J Biomed Sci. 2021;28:44 pubmed publisher
  2. Chen Z, Wang H, Wang S, Fan L, Feng S, Cai X, et al. USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells. J Clin Invest. 2019;130:2043-2055 pubmed publisher
  3. Luo H, Jing B, Xia Y, Zhang Y, Hu M, Cai H, et al. WP1130 reveals USP24 as a novel target in T-cell acute lymphoblastic leukemia. Cancer Cell Int. 2019;19:56 pubmed publisher
  4. Chen X, Yu C, Gao J, Zhu H, Cui B, Zhang T, et al. A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer. Theranostics. 2018;8:2348-2360 pubmed publisher
  5. Zhang S, Zhang M, Jing Y, Yin X, Ma P, Zhang Z, et al. Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitors. Nat Commun. 2018;9:215 pubmed publisher
  6. Naik E, Dixit V. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKCβ Kinase Activity. J Immunol. 2016;196:3438-51 pubmed publisher
  7. Esquerdo M, Grau Bove X, Garanto A, Toulis V, Garcia Monclús S, Millo E, et al. Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles. PLoS ONE. 2016;11:e0150364 pubmed publisher
  8. Kim M, Kim M, Park S, Lee C, Lim D. Role of Angiomotin-like 2 mono-ubiquitination on YAP inhibition. EMBO Rep. 2016;17:64-78 pubmed publisher
  9. Naik E, Webster J, DeVoss J, Liu J, Suriben R, Dixit V. Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X. J Exp Med. 2014;211:1947-55 pubmed publisher