This is a Validated Antibody Database (VAD) review about human USP5, based on 4 published articles (read how Labome selects the articles), using USP5 antibody in all methods. It is aimed to help Labome visitors find the most suited USP5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
USP5 synonym: ISOT; ubiquitin carboxyl-terminal hydrolase 5; deubiquitinating enzyme 5; isopeptidase T; testicular tissue protein Li 218; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ubiquitin-specific-processing protease 5

Proteintech Group
mouse monoclonal (3F12C10)
  • other; mouse; 1:500; loading ...; fig 6c
Proteintech Group USP5 antibody (Proteintech, 66213-1-lg) was used in other on mouse samples at 1:500 (fig 6c). Sci Rep (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-11)
  • western blot; human; loading ...; fig s6r
Santa Cruz Biotechnology USP5 antibody (Santa Cruz Biotechnology Inc, sc-390943) was used in western blot on human samples (fig s6r). Sci Adv (2018) ncbi
Bethyl
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4b
In order to develop a method to assess deubiquitylating enzyme activity in mouse tissues and use it to evaluate deubiquitylating enzyme inhibitors, Bethyl USP5 antibody (Bethyl, A301-542A) was used in western blot on mouse samples at 1:1000 (fig 4b). Methods Mol Biol (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 7
Bethyl USP5 antibody (Bethyl, A301-542A) was used in immunoprecipitation on human samples (fig 7). Sci Rep (2015) ncbi
Articles Reviewed
  1. Liu Q, Wu Y, Qin Y, Hu J, Xie W, Qin F, et al. Broad and diverse mechanisms used by deubiquitinase family members in regulating the type I interferon signaling pathway during antiviral responses. Sci Adv. 2018;4:eaar2824 pubmed publisher
  2. Juenemann K, Jansen A, van Riel L, Merkx R, Mulder M, An H, et al. Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies. Sci Rep. 2018;8:1405 pubmed publisher
  3. McLellan L, Forder C, Cranston A, Harrigan J, Jacq X. Activity Based Profiling of Deubiquitylating Enzymes and Inhibitors in Animal Tissues. Methods Mol Biol. 2016;1449:411-9 pubmed publisher
  4. Grou C, Pinto M, Mendes A, Domingues P, Azevedo J. The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors. Sci Rep. 2015;5:12836 pubmed publisher